Review
Zoology
Sergey Karakashev, Ru-Gang Zhang
Summary: Epithelial ovarian cancer is the leading cause of gynecological cancer-related mortality in developed countries, with multiple histological and molecular subtypes. Research focusing on animal models that consider the heterogeneity of the disease is crucial for studying novel therapies.
ZOOLOGICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Fabian Stahl, Ina Schmitt, Philip Denner, Laura de Boni, Ullrich Wuellner, Peter Breuer
Summary: The study suggests that simvastatin may be a potential treatment for SCA3 and increases ATXN3 protein levels by directly binding to the ATXN3 promoter.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Biomedical
I. Deniz Derman, Miji Yeo, Diana Cadena Castaneda, Megan Callender, Mian Horvath, Zengshuo Mo, Ruoyun Xiong, Elizabeth Fleming, Phylip Chen, Mark E. Peeples, Karolina Palucka, Julia Oh, Ibrahim T. Ozbolat
Summary: This study demonstrates the use of 3D bioprinting for the reconstruction of nasal epithelium, allowing high-throughput fabrication of tissue models. The progenitor human nasal epithelial cells were deposited on Transwell inserts using droplet-based bioprinting, resulting in the presence of cilia and tight-junctions, and recapitulating the pseudostratified columnar epithelial architecture.
Article
Cell & Tissue Engineering
Carlos A. Tristan, Pinar Ormanoglu, Jaroslav Slamecka, Claire Malley, Pei-Hsuan Chu, Vukasin M. Jovanovic, Yeliz Gedik, Yogita Jethmalani, Charles Bonney, Elena Barnaeva, John Braisted, Sunil K. Mallanna, Dorjbal Dorjsuren, Michael J. Iannotti, Ty C. Voss, Sam Michael, Anton Simeonov, Ilyas Singec
Summary: Efficient translation of human induced pluripotent stem cells (hiPSCs) into scalable cell manufacturing strategies was achieved through the establishment of a robotic platform. Automated essential steps of hiPSC culture and differentiation were conducted under chemically defined conditions. The approach enabled rapid and standardized production of billions of hiPSCs from various patient-and disease-specific cell lines, as well as automated multi-lineage differentiation into functional neurons, cardiomyocytes, and hepatocytes. Comprehensive molecular and cellular characterizations were performed to validate the robotic cell culture operations and benchmark industrial-scale cell culture for disease modeling, drug screening, and cell therapy.
Article
Chemistry, Analytical
Sang-Yun Lee, Hyun Ju Hwang, Bosung Ku, Dong Woo Lee
Summary: The use of the CPRE 3D-HTS model improves the correlation of EGFR-targeting drugs compared to 2D cell-based HTS. In breast cancer cell lines, the CPRE 3D-HTS model shows a greater difference in the expression of HER2 protein between HER2-positive and HER2-negative cells. Alginate was selected as the extracellular matrix to enhance cell proliferation receptor expression in the CPRE 3D-HTS model.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Mae W. Healy, Shelley N. Dolitsky, Maria Villancio-Wolter, Meera Raghavan, Alexandra R. Tillman, Nicole Y. Morgan, Alan H. DeCherney, Solji Park, Erin E. Wolff
Summary: The study successfully constructed 3-dimensional ovarian follicles using a microfluidic system, mimicking the necessary environment for early oocyte development. Somatic cell viability tests and hormonal assessments confirmed the cells' viability and endocrine function. The novel system can be easily adapted for sterile, high throughput applications.
Article
Chemistry, Analytical
Chunyang Wei, Chengzhuang Yu, Shanshan Li, Tiejun Li, Jiyu Meng, Junwei Li
Summary: The presented microfluidic chip provides a possible solution for achieving 3D cell culture and enabling scalable and high-throughput cell culture, with great potential for application in biological fields.
Review
Biochemistry & Molecular Biology
Alessandra Ciucci, Marianna Buttarelli, Anna Fagotti, Giovanni Scambia, Daniela Gallo
Summary: This review discusses various experimental models in ovarian cancer research, including cell line cultures, organoids, animal models, and tumor-on-a-chip platforms. Evaluating the strengths and weaknesses of each model will contribute to optimizing their translational value.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Chemistry, Physical
Vei Wang, Gang Tang, Ya-Chao Liu, Ren-Tao Wang, Hiroshi Mizuseki, Yoshiyuki Kawazoe, Jun Nara, Wen Tong Geng
Summary: Two-dimensional materials have attracted attention for their unique physical properties and their ability to meet the demands of nanoscale devices. In this study, we conducted high-throughput calculations and screening to identify 73 direct-gap and 183 indirect-gap 2D nonmagnetic semiconductors based on criteria for thermodynamic, mechanical, dynamic, and thermal stabilities, as well as conductivity type. A database of these materials was created, providing a platform for computational modeling and design of new 2D semiconductors and heterostructures in applications such as photocatalysis and nanoscale devices. Additionally, a linear fitting model was proposed to accurately evaluate band gap, ionization energy, and electron affinity of 2D semiconductors with lower computational cost compared to hybrid DFT methods.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Physical
Mads Kruse, Urko Petralanda, Morten N. Gjerding, Karsten W. Jacobsen, Kristian S. Thygesen, Thomas Olsen
Summary: We performed a high throughput computational search in the C2DB and identified 63 ferroelectric materials, including 49 with in-plane polarization, 8 with out-of-plane polarization, and 6 with coupled in-plane and out-of-plane polarization. Most known 2D ferroelectrics were recovered and the predicted ones are accessible by direct exfoliation. About 25% of the materials showed a metastable state in the non-polar structure. We also discussed the case of VAgP2Se6, a magnetic pyroelectric material with switchable polarization coupled to magnetic excitation spectrum.
NPJ COMPUTATIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Maobi Zhu, Na Wang, Sha Wang, Yao Wang, Xiawen Yang, Jianglin Fan, Yajie Chen
Summary: This study examined the injury and repair mechanism of the fallopian tube, the original site of high-grade serous ovarian carcinoma. The results showed that follicular fluid stimulation promotes cell differentiation and organoid formation, but also causes cell injury and apoptosis at high concentrations. These findings contribute to the investigation of the initiation mechanisms of high-grade serous ovarian carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Chunqi Chang, Haoyi Yang, Wenchuan Bi, Cuier Huang, Zhen Xu
Summary: We developed a simple colorimetric assay for studying cancer cell viability and migration. The assay involves automatic liquid sample loading, high-throughput generation of chemical concentration gradient, three-dimensional cell culture, and smartphone-based colorimetric readouts. This assay allows for the study of cell viability and migration using one device, and it is easy to use in common laboratories or clinical settings.
Article
Chemistry, Multidisciplinary
Bryan Queme, John C. Braisted, Patricia Dranchak, James Inglese
Summary: High throughput screening (HTS) is widely used in drug discovery and chemical biology to identify and characterize agents. Standard HTS data is usually represented as an x-y graph, while quantitative HTS (qHTS) data incorporates a third axis represented by concentration. qHTS data is challenging to display on a single graph due to the additional data points and logistic fit parameters.
JOURNAL OF CHEMINFORMATICS
(2023)
Review
Biotechnology & Applied Microbiology
Manman Sun, Alex Xiong Gao, Xiuxia Liu, Yankun Yang, Rodrigo Ledesma-Amaro, Zhonghu Bai
Summary: The demand for faster and more efficient protein production has increased in the post-genomic era. This review presents a holistic high-throughput process development strategy and discusses the challenges, limitations, and future research directions in recombinant protein production.
MICROBIAL CELL FACTORIES
(2023)
Article
Reproductive Biology
Li Dong, Xin Xin, Hsun-Ming Chang, Peter C. K. Leung, Chen Yu, Fang Lian, Haicui Wu
Summary: This study compared the expression profiles of lncRNA and mRNA in ovarian granulosa cells (OGCs) between patients with normal ovarian reserve (NOR) and diminished ovarian reserve (DOR) using RNA sequencing. The identified lncRNAs and mRNAs may serve as novel diagnostic biomarkers for patients with DOR.
JOURNAL OF OVARIAN RESEARCH
(2022)