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Geriatrics & Gerontology
Rui Liang, Xiaopeng Shen, Fan Wang, Xin Wang, Alex DesJarlais, Anam Syed, Raymond Saba, Zhi Tan, Fang Yu, Xuan Ji, Shreesti Shrestha, Yinghong Ren, Jin Yang, Yoonjung Park, Robert J. Schwartz, Benjamin Soibam, Bradley K. McConnell, M. David Stewart, Ashok Kumar, Yu Liu
Summary: The study reveals that H19X microRNAs regulate skeletal muscle growth and atrophy by controlling the abundance of translation initiation factors, affecting protein synthesis. It highlights the crucial role of translation initiation factors in multiple signaling pathways that control skeletal muscle mass.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Cell Biology
Jiaqing Zhang, Qiaoling Ren, Junfeng Chen, Lingyan Lv, Jing Wang, Ming Shen, Baosong Xing, Xianwei Wang
Summary: This study investigated the impact of oxidative stress on porcine granulosa cells (PGCs) through miRNA-Seq analysis. The findings revealed that miR-192 was the most induced miRNA during H2O2-mediated oxidative stress, and downregulation of miR-192 alleviated oxidative injury. Additionally, treatment with grape seed procyanidin B2 (GSPB2) reduced oxidative damage by downregulating miR-192, upregulating Acvr2a expression, and suppressing the caspase-3 apoptotic signaling pathway.
Article
Biochemistry & Molecular Biology
Erin A. Nekritz, Ruth Rodriguez-Barrueco, Koon-Kiu Yan, Meredith L. Davis, Rachel L. Werner, Laura Devis-Jauregui, Partha Mukhopadhyay, Jiyang Yu, David Llobet-Navas, Jose Silva
Summary: The miR-424/503 cluster plays a crucial role in regulating Wnt signaling, MaSC fate, and breast cancer development, with its function involving targeting the LRP6 co-receptor to reduce its expression.
Article
Cell Biology
Victoria Zyulina, Koon-Kiu Yan, Bensheng Ju, Elke Schwarzenberger, Christina Passegger, Carmen Tam-Amersdorfer, Qingfei Pan, Tommaso Sconocchia, Christian Pollack, Bridget Shaner, Armin Zebisch, John Easton, Jiyang Yu, Jose M. Silva, Herbert Strobl
Summary: The TGF-beta family ligands play a crucial role in dendritic cell differentiation and activation. Through investigating differentially expressed microRNAs, it was found that miR-424/503 is significantly inversely regulated between moDCs and LCs. Further study revealed that miR-424/503 induction promotes moDC differentiation, while its repression facilitates TGF-beta 1-dependent LC differentiation from monocytes.
Article
Biotechnology & Applied Microbiology
Jiewen Su, Dawei Chen, Yi Ruan, Yuan Tian, Kaiji Lv, Xinhua Zhou, Dongjian Ying, Yeting Lu
Summary: Studies have shown that lncRNA MBNL1-AS1 participates in the progression of gastric cancer by regulating the miR-424-5p/Smad7 axis, thus activating the TGF-beta/EMT pathways. This finding may provide a potential marker for gastric cancer patients.
Article
Reproductive Biology
Shen Tian, Han Zhang, Hsun-Ming Chang, Christian Klausen, He-Feng Huang, Min Jin, Peter C. K. Leung
Summary: Activin A increases the production of hyaluronan and the expression of versican in human granulosa-lutein cells, possibly through the mediation of the ALK4/SMAD2/SMAD3-SMAD4 signaling pathway.
BIOLOGY OF REPRODUCTION
(2022)
Article
Oncology
Xiao-Song Wang, Xiao-Jun Yu, Kang Wei, Shan-Xi Wang, Qi-Kun Liu, Ying-Guang Wang, Han Li, Cheng Huang
Summary: This study discovered that miR-503 incorporated in MSC-EVs acts as a regulator in controlling immune escape in glioma. These findings provide a novel molecular mechanism for developing therapeutic strategies against glioma.
Article
Biochemistry & Molecular Biology
Reem Sabry, Makenna Williams, Nicholas Werry, Jonathan LaMarre, Laura A. Favetta
Summary: This study investigates the regulation of miR-21 in bovine granulosa cells and its potential mechanisms. The research shows that miR-21 has anti-apoptotic effects and explains its impact on specific apoptotic targets. Additionally, the study confirms the influence of BPA on miR-21 expression and explores the temporal effects of BPA on miR-21 regulation. Furthermore, the research reveals that BPA induces upregulation of miR-21 expression by modulating upstream regulators. Overall, this study enhances our understanding of the function of miR-21 in granulosa cells and the mechanisms underlying BPA-induced reproductive impairment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Shuo Wang, Weicheng Tang, Lanfang Ma, Jun Yang, Kecheng Huang, Xiaofang Du, Aiyue Luo, Wei Shen, Ting Ding, Shuangmei Ye, Su Zhou, Shuhong Yang, Shixuan Wang
Summary: The study demonstrated that miR-145 targets Crkl and promotes mouse granulosa cells proliferation, differentiation, and steroidogenesis through the JNK/p38 MAPK signaling pathway. MiR-145 may play a significant role in ovarian physiology and pathology.
Article
Reproductive Biology
Yongjin Bao, Xiaodan Li, M. A. El-Samahy, Hua Yang, Zhibo Wang, Fan Yang, Xiaolei Yao, Feng Wang
Summary: Activin/inhibin is important for Hu sheep fecundity, involved in ovarian follicular development. INHBA participates in activin A and inhibin A synthesis. Positive correlation noted between INHBA level and activin A/inhibin A secretion. INHBA knockdown/overexpression downregulated INHA expression. RNA-Sequencing revealed 1,687 DEGs (Fold change >= 2, FDR < 0.01), including upregulated and downregulated genes in INHBA interference group. GO enrichment showed involvement in cell cycle regulation, protein serine/threonine kinase activity, and actin cytoskeleton reorganization. DEGs significantly enriched in 40 KEGG pathways, including P53, progesterone-mediated oocyte maturation, and PI3K-AKT signaling pathways. Positive correlation between INHBA level and PI3K/Akt/mTOR pathway-related genes/proteins. Study enhances understanding of INHBA roles in prolific sheep GCs and molecular effects of low INHBA expression on GCs, clarifying reproductive failures.
Article
Cell Biology
Shengjie Shi, Yamei Hu, Xiangrong Song, Liang Huang, Lutong Zhang, Xiaoge Zhou, Lei Gao, Weijun Pang, Gongshe Yang, Guiyan Chu
Summary: miR-184 plays a key role in the physiological functions of granulosa cells by promoting E-2 synthesis and cell proliferation, and inhibiting apoptosis through targeting three genes.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Genetics & Heredity
Anwar Abdurahman, Wusimanjiang Aierken, Fei Zhang, Rahmantay Obulkasim, Jueken Aniwashi, Ablat Sulayman
Summary: In this study, it was shown that miR-1306 directly inhibits the BMPR1B gene in ovine granulosa cells, leading to increased cell apoptosis. This finding suggests that miR-1306 could be a potential target for improving sheep prolificacy.
FRONTIERS IN GENETICS
(2022)
Article
Oncology
Reem Sabry, Makenna Williams, Jonathan LaMarre, Laura A. Favetta
Summary: This study found that Bisphenol A (BPA) induces apoptosis in bovine granulosa cells (GCs) through the activation of several intrinsic factors, but miR-21 is not involved in the toxic effects of BPA.
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Cell Biology
Reem Sabry, Diana R. May, Laura A. Favetta
Summary: This study reveals a strong correlation between miR-21 signaling and the regulation of DNA methylation in bovine Cumulus-Oocyte-Complexes (COCs) and granulosa cells under BPA exposure.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Dong Yuan, Jing Luo, Yixuan Sun, Lijuan Hao, Jing Zheng, Zhu Yang
Summary: Polycystic ovary syndrome (PCOS) is characterized by increased ovarian androgen biosynthesis, chronic anovulation, and polycystic ovaries. MiR-424-5p expression is significantly decreased in PCOS exosomes and primary granulosa cells, leading to inhibition of granulosa cells proliferation and induction of cellular senescence. Overexpression of CDCA4 reverses the effects of miR-424-5p by activating the Rb/E2F1 signaling pathway.
CELLULAR SIGNALLING
(2021)
Article
Cell Biology
Hoda Samir Aglan, Samuel Gebremedhn, Dessie Salilew-Wondim, Christiane Neuhof, Ernst Tholen, Michael Holker, Karl Schellander, Dawit Tesfaye
CELL AND TISSUE RESEARCH
(2020)
Article
Agriculture, Dairy & Animal Science
Omar Khadrawy, Samuel Gebremedhn, Dessie Salilew-Wondim, Franca Rings, Christiane Neuhoff, Michael Hoelker, Karl Schellander, Dawit Tesfaye
REPRODUCTION IN DOMESTIC ANIMALS
(2020)
Article
Cell Biology
A. M. Zolini, J. Block, M. B. Rabaglino, G. Rincon, M. Hoelker, J. J. Bromfield, D. Salilew-Wondim, P. J. Hansen
CELL AND TISSUE RESEARCH
(2020)
Article
Multidisciplinary Sciences
Samuel Gebremedhn, Ahmed Gad, Hoda Samir Aglan, Jozef Laurincik, Radek Prochazka, Dessie Salilew-Wondim, Michael Hoelker, Karl Schellander, Dawit Tesfaye
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Mohamed Omar Taqi, Mohammed Saeed-Zidane, Samuel Gebremedhn, Dessie Salilew-Wondim, Ernst Tholen, Christiane Neuhoff, Michael Hoelker, Karl Schellander, Dawit Tesfaye
Summary: This study found that bovine granulosa cells exposed to oxidative stress induced by H2O2 exhibited differential expression of various transcription factors, which are mediated by the NRF2 signaling pathway.
CELL AND TISSUE RESEARCH
(2021)
Article
Cell Biology
Eryk Andreas, Hari Om Pandey, Michael Hoelker, Dessie Salilew-Wondim, Samuel Gebremedhn, Karl Schellander, Dawit Tesfaye
Summary: Dynamic changes in microRNAs in oocyte and cumulus cells during bovine follicular cells' maturation process, particularly miR-20a, play a crucial role in regulating proliferation, differentiation, and progesterone synthesis. Increasing miR-20a expression in cumulus cells, while decreasing it in oocytes, enhances oocyte maturation. Additionally, miR-20a regulates oocyte maturation by suppressing BMPR2 expression and increasing cumulus cell progesterone synthesis.
Article
Biotechnology & Applied Microbiology
Dessie Salilew-Wondim, Dawit Tesfaye, Franca Rings, Eva Held-Hoelker, Dennis Miskel, Marc-Andre Sirard, Ernst Tholen, Karl Schellander, Michael Hoelker
Summary: The study compared the transcriptome profiles of in vivo and in vitro derived bovine blastocysts, revealing the effects of developmental competence and environmental conditions on gene expression outlines. It also identified differentially expressed genes and pathways reflective of developmental capacities, suggesting that transcriptome activity in blastocysts may indicate future success in pregnancy and adaptation to culture environment.
Article
Biochemistry & Molecular Biology
Hoda Samir Aglan, Marwa M. Safar, Afaf Abdel-Moniem Ain-Shoka, Asmaa Munir Kandil, Samuel Gebremedhn, Dessie Salilew-Wondim, Karl Schellander, Dawit Tesfaye
Summary: Exposure to lead resulted in various adverse effects on pregnant rats and their fetuses, including decreased maternal body weight, placental weight, and fetal abnormalities. However, taurine showed partial protective effects against these impacts.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2021)
Article
Multidisciplinary Sciences
Dennis Miskel, Mikhael Poirier, Luisa Beunink, Franca Rings, Eva Held, Ernst Tholen, Dawit Tesfaye, Karl Schellander, Dessie Salilew-Wondim, Carina Blaschka, Christine Grosse-Brinkhaus, Brenig Bertram, Michael Hoelker
Summary: This study reveals that adjusting RNP-electroporation relative to the zygote cell cycle significantly affects mosaicism in Full-Edit embryos. A considerable proportion of Full Edit embryos demonstrated loss-of-heterozygosity after RNP-electroporation, which may be a consequence of chromosomal disruptions. However, not all loss-of-heterozygosity events are associated with loss of an entire chromosome or chromosomal sections.
SCIENTIFIC REPORTS
(2022)
Correction
Multidisciplinary Sciences
Dennis Miskel, Mikhael Poirier, Luisa Beunink, Franca Rings, Eva Held, Ernst Tholen, Dawit Tesfaye, Karl Schellander, Dessie Salilew-Wondim, Carina Blaschka, Christine Groe-Brinkhaus, Bertram Brenig, Michael Hoelker
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Maria Belen Rabaglino, Dessie Salilew-Wondim, Adriana Zolini, Dawit Tesfaye, Michael Hoelker, Pat Lonergan, Peter J. J. Hansen
Summary: This study developed a gene signature model to predict embryonic competence for survival by integrating transcriptomic data from blastocysts and elongating conceptuses. The potential biomarkers were validated with independent embryonic data sets using machine-learning algorithms. The results showed that the predictions of embryonic development fate based on these biomarkers were highly accurate.
Article
Multidisciplinary Sciences
Bekalu Muluneh, Mengistie Taye, Tadelle Dessie, Dessie Salilew Wondim, Damitie Kebede, Andualem Tenagne
Summary: This study characterized and analyzed the morphological and biometric traits of indigenous chickens in northwestern Ethiopia, finding that plumage color and shank color were the most significant traits. Gender, agro-ecology, location, and the interaction of gender and location had a significant impact on body measurements. Cluster analysis classified the indigenous chickens into four ecotypes in the study area.
Correction
Cell Biology
A. M. Zolini, J. Block, M. B. Rabaglino, G. Rincon, M. Hoelker, J. J. Bromfield, D. Salilew-Wondim, P. J. Hansen
CELL AND TISSUE RESEARCH
(2020)