Article
Microbiology
Hee-seung Han, Haeji Yum, Young-Dan Cho, Sungtae Kim
Summary: This study aimed to examine the effects of Weissella cibaria Chonnam Medical University (CMU)-containing tablets on halitosis. The results showed that the use of tablets containing W. cibaria CMU can effectively reduce the concentration of volatile sulfur compounds, improve bad breath scores, and improve psychological indicators in patients with halitosis.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Immunology
Cho Eun Kang, Yeong Jin Park, Ji Hun Kim, Na-Kyoung Lee, Hyun-Dong Paik
Summary: This study evaluated the antibacterial and antibiofilm effects of four Weissella cibaria strains on three Streptococcus mutans strains. The results showed that W. cibaria reduced exopolysaccharides production and auto-aggregation, increased co-aggregation, and downregulated virulence factors, leading to the inhibition of bacterial growth and biofilm formation. These findings were confirmed using scanning electron microscopy and confocal laser scanning microscopy. This study suggests that W. cibaria has the potential to improve oral health.
MICROBIAL PATHOGENESIS
(2023)
Article
Microbiology
Yuqi Gao, Mingze Niu, Xiaohui Yu, Tingting Bao, Zhaowei Wu, Xin Zhao
Summary: A newly identified EPS-synthetic gene cluster in Lactiplantibacillus plantarum plays a crucial role in enhancing biofilm formation ability, tolerance to harsh environmental stresses, adhesion to intestinal epithelial cells, as well as attenuating inflammation and inhibiting pathogenic bacteria adhesion. This finding highlights the significance of mobile EPS-synthetic elements in the probiotic activity of L. plantarum and provides insights for probiotic bacteria engineering.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Annel M. Hernandez-Alcantara, Rosana Chiva, Maria Luz Mohedano, Pasquale Russo, Jose Angel Ruiz-Maso, Gloria Del Solar, Giuseppe Spano, Mercedes Tamame, Paloma Lopez
Summary: This work describes a method for obtaining riboflavin overproducing strains of Weissella cibaria through exposure to increasing concentrations of roseoflavin. Mutations in the rib operons of the mutant strains were identified as the molecular basis for riboflavin overproduction. The production of dextran, another product of W. cibaria, was not affected by these mutations. The mutant strains were capable of producing biofortified breads with both riboflavin and dextran during experimental bread making.
FRONTIERS IN NUTRITION
(2022)
Article
Biotechnology & Applied Microbiology
Mi-Sun Kang, Geun-Yeong Park
Summary: This study evaluated the efficacy of oraCMS1's cell-free supernatant (CFS) in inactivating RSV, H1N1, and RVA. The results demonstrated that the CFS of oraCMS1 can effectively inactivate these viruses, thereby reducing the risk of respiratory tract and intestinal infections.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2022)
Article
Microbiology
Seoyoung Jeong, Ayeon Kwon, Huijin Jeong, Young-Seo Park
Summary: This study found that the synbiotic combinations of probiotics and prebiotics have higher immunostimulatory activities on macrophages compared to the corresponding probiotic strains and oligosaccharides alone. The synergistic effect of probiotics and the prebiotics in the studied synbiotic preparations is due to the activation of the mitogen-activated protein-kinase-signaling pathway. This suggests the potential use of synbiotic preparations as health supplements.
Article
Microbiology
Inaki Diez-Ozaeta, Lucia Martin-Loarte, Mari Luz Mohedano, Mercedes Tamame, Jose Angel Ruiz-Maso, Gloria del Solar, Maria Teresa Duenas, Paloma Lopez
Summary: This study developed a new method to select riboflavin-overproducing strains of Weissella cibaria using resistance to roseoflavin as a marker. DNA sequencing revealed mutations in the FMN riboswitch, potentially deregulating riboflavin synthesis. The selected mutants showed promising riboflavin production and potential applications in the food and health industry.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Soyoung Park, Kandasamy Saravanakumar, Anbazhagan Sathiyaseelan, Ki-Seok Han, Jooeun Lee, Myeong-Hyeon Wang
Summary: Supplementation of Weissella cibaria EPS promotes the growth and antibacterial properties of Lactobacillus rhamnosus, increasing colony-forming unit count without affecting morphology.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Francesca Fanelli, Marco Montemurro, Michela Verni, Antonella Garbetta, Anna Rita Bavaro, Daniele Chieffi, Gyu-Sung Cho, Charles M. A. P. Franz, Carlo Giuseppe Rizzello, Vincenzina Fusco
Summary: The study analyzed the safety and functional features of six Weissella and Periweissella strains, finding that the P. beninensis strain has the highest potential as a probiotic based on its features and safety assessment.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Mi-Sun Kang, Geun-Yeong Park
Summary: Oral probiotics, specifically W. cibaria CMU and CMS1, were found to inhibit the formation of multi-species biofilms on titanium implant surfaces. This suggests that these probiotics could be a promising approach for preventing peri-implant mucositis.
Article
Food Science & Technology
Camelia Filofteia Diguta, Constanta Mihai, Radu Cristian Toma, Carmen Cimpeanu, Florentina Matei
Summary: This study aimed to investigate the probiotic potential of three yeast strains isolated from agro-food natural sources, comparing them with a reference Saccharomyces boulardii. The yeast strains showed tolerance to different temperatures, salt concentrations, and pH ranges. S. cerevisiae BB06 exhibited strong antagonistic activity, high auto-aggregation ability, good surface hydrophobicity, and excellent antioxidant properties. The yeast strains also demonstrated resistance to antibacterial antibiotics and high survival rates during freeze-drying. Among them, S. cerevisiae BB06 was found to have valuable probiotic traits.
Article
Food Science & Technology
Ewelina Maslak, Michal Zloch, Adrian Arendowski, Mateusz Sugajski, Izabela Janczura, Joanna Rudnicka, Justyna Walczak-Skierska, Magdalena Buszewska-Forajta, Katarzyna Rafinska, Pawel Pomastowski, Dorota Bialczak, Boguslaw Buszewski
Summary: This study isolated two strains of lactic acid bacteria from a pickled beetroot sample, which showed potential to be considered as probiotics in the future. The strains were tested for their growth conditions, proteolytic properties, lipid profile, ability to synthesize beneficial metabolites, and inhibition of pathogenic strains. The results showed that these strains have the desired properties for use as starter cultures in functional food production.
Article
Multidisciplinary Sciences
Diletta Mazzantini, Marco Calvigioni, Francesco Celandroni, Antonella Lupetti, Emilia Ghelardi
Summary: The study found that different strains have different in vitro biological properties, highlighting the usefulness of in vitro tests as a prelude for clinical research.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Pochanart Kanjan, Apisit Kimtun, Saweit Chaimongkol, Phat Sakpetch
Summary: The antibacterial activity of Weissella cibaria KY10 derived from the shrimp digestive tract against Vibrio parahaemolyticus T.11 causing AHPND was investigated. The study found that W. cibaria KY10 completely inhibited the growth of V. parahaemolyticus T.11 and produced antibacterial substances, mainly consisting of higher-pKa acids. In addition, this strain showed strong auto-aggregation, hydrophobicity, and tolerance to gastric juice and bile salt. Importantly, it had no haemolysis or pathogenic effects in vivo. These findings suggest that W. cibaria KY10 has potential as an autochthonous probiotic for controlling V. parahaemolyticus causing AHPND in shrimp aquaculture.
AQUACULTURE RESEARCH
(2022)
Article
Microbiology
V. B. Chandana Kumari, Sujay S. Huligere, Ramith Ramu, Shrisha Naik Bajpe, M. Y. Sreenivasa, Ekaterina Silina, Victor Stupin, Raghu Ram Achar
Summary: Fermented foods contain beneficial microbes that can regulate biomolecule metabolism, offering various health benefits. This study characterized and validated lactic acid bacteria species isolated from fermented beetroot, confirming their probiotic activity. The isolates exhibited high survival rates, adherence capability, colonization, antibacterial and antibiotic activity, and potential as functional food and health-related products. The findings also highlighted their exceptional antidiabetic potential, making them valuable for further research in this field.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Pavan Thapak, Pragyanshu Khare, Mahendra Bishnoi, Shyam Sunder Sharma
Summary: The study suggests that 2-APB has a neuroprotective effect in A beta-induced cognitive impairment. It decreases the expression of TRPM2, improves cognitive function, and regulates calcium and memory-related proteins.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Immunology
Priyanka Saroj, Yashika Bansal, Raghunath Singh, Ansab Akhtar, Rupinder Kaur Sodhi, Mahendra Bishnoi, Sangeeta Pilkhwal Sah, Anurag Kuhad
Summary: The study demonstrated that roflumilast treatment significantly improved locomotor activity, motor coordination, and gait performance in rats. It also reduced oxidative and nitrosative stress, decreased pro-inflammatory cytokines, and increased BDNF levels in the striatum and cortex of the rat brain. Furthermore, roflumilast effectively modulated the gene expression of cAMP, CREB, and PDE4, suggesting its potential as a therapeutic agent for Huntington's disease management through the cAMP/CREB/BDNF signaling pathway.
INFLAMMOPHARMACOLOGY
(2021)
Article
Neurosciences
Pavan Thapak, Mahendra Bishnoi, Shyam Sunder Sharma
Summary: Research has shown that the expression of TRPV2 channels is upregulated in Alzheimer's disease models, and is associated with cognitive impairment. Treatment with tranilast can improve cognitive parameters by reducing TRPV2 expression and AChE activity.
NEUROMOLECULAR MEDICINE
(2022)
Article
Medicine, Research & Experimental
Vijay Kumar, Vibhu Kumar, Neha Mahajan, Jasleen Kaur, Kirti Devi, Ravinder Naik Dharavath, Ravindra Pal Singh, Kanthi Kiran Kondepudi, Mahendra Bishnoi
Summary: The study found that taking capsaicin and mucin had similar anti-obesity effects, indicating that mucin modulation might play a crucial role in the anti-obesity effects of capsaicin. 16S rDNA sequencing and in vitro Ca2+ measurement experiments suggested that bacteria, in addition to capsaicin, are involved in TRPV1 modulation.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Food Science & Technology
Ravindra Pal Singh, Jayashree Niharika, Kanthi Kiran Kondepudi, Mahendra Bishnoi, Jagan Mohan Rao Tingirikari
Summary: Human milk oligosaccharides (HMOs) are complex sugars present in breast milk, playing crucial roles in infant development by providing energy for gut microbes and directly modulating gut immune responses. Additionally, HMOs protect infants from infectious diseases by interacting with intestinal cells and inhibiting the binding of pathogenic bacteria and viruses.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Endocrinology & Metabolism
Jasleen Kaur, Vijay Kumar, Vibhu Kumar, Sadiah Shafi, Pragyanshu Khare, Neha Mahajan, Sanjay K. Bhadada, Kanthi Kiran Kondepudi, Rupam Kumar Bhunia, Anurag Kuhad, Mahendra Bishnoi
Summary: The study demonstrated that supplementation of a tri-agonist consisting of capsaicin, menthol, and cinnamaldehyde at sub-effective doses prevented weight gain, improved glucose homeostasis, and inhibited adipose tissue hypertrophy induced by a high-fat diet in mice. This approach may be used to develop functional foods for therapeutic and preventive strategies against complications associated with high-fat diet consumption.
INTERNATIONAL JOURNAL OF OBESITY
(2022)
Review
Biotechnology & Applied Microbiology
Prakriti Vats, Ujjwal Jit Kaur, Praveen Rishi
Summary: Antibiotic resistance is a global threat, driven not only by the improper use of antibiotics but also by the selection pressure from other antimicrobial agents like heavy metals. Heavy metal contamination contributes to the development of antibiotic and metal resistance through mechanisms such as co-resistance and cross-resistance. However, there is a scarcity of studies investigating the process of co-selection in clinical settings. Prospective strategies are needed to manage heavy metal-induced antibiotic resistance, including the search for specific inhibitors and the addition of alternative medicinal combinations to existing treatment options.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Nutrition & Dietetics
Dharmisthaben Patel, Amar Sakure, Dikshita Lodha, Bethsheba Basaiawmoit, Ruchika Maurya, Sujit Das, Mahendra Bishnoi, Kanthi Kiran Kondepudi, Subrota Hati
Summary: This study aimed to assess the bio-functional properties of camel milk, including its antioxidative and anti-inflammatory activities, through fermentation with Lactobacillus fermentum strain KGL4. The study also identified and characterized the bioactive peptides released during fermentation. The results showed that the fermented camel milk exhibited high antioxidant activity and the KGL4 strain produced novel antioxidative peptides.
JOURNAL OF THE AMERICAN NUTRITION ASSOCIATION
(2023)
Review
Biotechnology & Applied Microbiology
Harmanpreet Kaur, Arashdeep Kaur, Sanjeev Kumar Soni, Praveen Rishi
Summary: Enzymes, known as biocatalysts, have properties like high substrate specificity and eco-friendliness, and are increasingly investigated for their anti-biofouling action in healthcare, food manufacturing, and environmental applications. Carbohydrase consortiums offer an effective alternative to conventional chemical anti-biofouling methods by targeting heterogeneous polysaccharides in the EPS matrix for complete biofilm eradication.
Article
Biotechnology & Applied Microbiology
Khem Raj, Praveen Rishi, Geeta Shukla, Shivaprakash M. Rudramurhty, Dattatray S. Mongad, Amrita Kaur
Summary: This study investigates the expression and usage of alternative transcript isoforms in Candida glabrata, a eukaryotic opportunistic pathogen. The analysis reveals significant differences in transcript isoform expression and usage during mature biofilm growth. These findings suggest that alternative transcript isoforms play an important role in biofilm formation in C. glabrata.
INDIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vibhu Kumar, Vijay Kumar, Kirti Devi, Ajay Kumar, Rehan Khan, Ravindra Pal Singh, Sivasubramanian Rajarammohan, Kanthi Kiran Kondepudi, Kanwaljit Chopra, Mahendra Bishnoi
Summary: The study investigated the effects of TRPA1/TRPV1 modulation on colonic mucus production and gut health. It found that modulation of TRPA1/TRPV1 resulted in damaged mucosal lining, increased gut permeability, and altered transcriptional profile of related genes. These results suggest caution should be exercised when considering TRPA1/TRPV1 modulation as a therapeutic option for pain caused by IBD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kirti Devi, Vibhu Kumar, Vijay Kumar, Neha Mahajan, Jasleen Kaur, Shikha Sharma, Ajay Kumar, Rehan Khan, Mahendra Bishnoi, Kanthi Kiran Kondepudi
Summary: Chemical-enzymatic processing of finger millet, kodo millet, and rice bran removes lipids and antinutrients, making them effective in preventing high fat diet-induced obesity and improving glucose homeostasis and gut health.
Article
Immunology
Anayata Sharma, Praveen Rishi, Rachna Singh
Summary: This study evaluated the efficacy of DNase I in improving the activity of cefotaxime, amikacin, and ciprofloxacin against biofilms formed by Klebsiella pneumoniae. The results showed that DNase I enhanced the biofilm-eradicating capacity of ciprofloxacin and significantly reduced the presence of biofilms.
PATHOGENS AND DISEASE
(2023)
Article
Chemistry, Medicinal
Vibhu Kumar, Vijay Kumar, Kanthi Kiran Kondepudi, Kanwaljit Chopra, Mahendra Bishnoi
Summary: The study aimed to investigate the effects of altered colonic mucosal health and high-fat diet in mice. It also examined whether this combination prevented the protective actions of dietary prebiotic, isomaltooligosaccharides. The study found that the combination of altered colonic mucosal health and high-fat diet exacerbated the damage to mucosal lining, intestinal permeability, tight junction protein expression, SCFA levels, and gut bacterial abundances. The findings suggest that colonic precondition is crucial in determining the impact of high-fat diet and the success of diet-associated interventions.
ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Arif Hassan Dar, Anas Ahmad, Ajay Kumar, Vijayendran Gowri, Chandrashekhar Jori, Shaifali Sartaliya, K. M. Neethu, Nemat Ali, Mahendra Bishnoi, Rehan Khan, Govindasamy Jayamurugan
Summary: This study focuses on the development of luminescent dyes based on TCBDs and their transformation into water-soluble nanoaggregates. These nanoaggregates exhibit high emissivity and have potential applications in bioimaging and photodynamic therapy, specifically targeting cancer cells and increasing cell death activity.
Article
Biochemistry & Molecular Biology
Davide Risso, Gillian Dunngalvin, Sameer Saxena, Andrea Doolan, Lisa Spence, Kavita Karnik
Summary: This study investigated the gastrointestinal tolerance of low-calorie sugar D-allulose in young children and found that it was well tolerated. It can be considered as a candidate for replacing added sugars with lower caloric content in commercially produced goods.
Article
Biochemistry & Molecular Biology
Xinying Suo, Anna Baggio, Nicoletta Pellegrini, Silvia Vincenzetti, Elena Vittadini
Summary: This study investigated the effects of shape, gluten, and structural breakdown on the starch digestibility and glycemic index of gluten-containing and gluten-free pasta. The results showed that mastication effort, shape, and gluten content had significant impacts on starch digestion and glycemic index, with gluten being the major factor affecting the amount of digested starch.
Article
Biochemistry & Molecular Biology
Xinnuo Hu, Yanjun Yang, Cuihua Chang, Junhua Li, Yujie Su, Luping Gu
Summary: Fish collagen was enzymatically hydrolyzed using alkaline, papain, and ginger protease. The addition of ginger protease increased the degree of hydrolysis and resulted in changes in the amino acid composition and molecular weight distribution of the peptides. Combined hydrolysis with ginger protease improved the ACE inhibitory activity of the sample but affected fibroblast proliferation activity.
Article
Biochemistry & Molecular Biology
Tong Nie, Xin Wang, Aqun Li, Anshan Shan, Jun Ma
Summary: This study investigated the mechanism by which hesperidin prevents nonalcoholic fatty liver disease (NAFLD) by modulating fatty acid beta oxidation. The results suggest that hesperidin promotes fatty acid beta oxidation by activating SIRT1/PGC1 alpha, thus improving NAFLD.
Article
Biochemistry & Molecular Biology
Li Li, Jing Zhao, Jinxue Wang, Qianqian Xiong, Xuechun Lin, Xiaolei Guo, Fan Peng, Wangqun Liang, Xuezhi Zuo, Chenjiang Ying
Summary: The daily administration of 10 g d-1 inulin-type fructans significantly reduced the circulating arsenic retention in end-stage renal disease by increasing the arsenic clearance rate through urine and dialysis. However, it was found to be ineffective for other trace elements.
Article
Biochemistry & Molecular Biology
Chuanchuan Wang, Weixuan Chen, Yishan Jiang, Xiao Xiao, Qianhui Zou, Jiarui Liang, Yu Zhao, Qianxu Wang, Tian Yuan, Rui Guo, Xuebo Liu, Zhigang Liu
Summary: Autism Spectrum Disorder (ASD) symptoms may be improved through modulation of gut microbiota. This study investigated the effects of a synbiotic treatment on an ASD-like mouse model, and found that it rectified social impairments, attenuated inflammatory cytokine expressions, protected gut barrier integrity, and altered gut microbiota composition. The synbiotic treatment elevated beneficial metabolites and upregulated genes associated with their synthesis. Overall, the synbiotic combination mitigated ASD-related social impairments through regulation of the gut-brain axis.
Review
Biochemistry & Molecular Biology
Chunli Kong, Caiping Duan, Yixuan Zhang, Yiying Wang, Zheng Yan, Sumei Zhou
Summary: This review critically discusses the physicochemical properties of bioactive molecules in kidney beans and focuses on the current knowledge of non-starch polysaccharide (NSP) structure and function on intestinal health and metabolism. It also provides suggestions for further investigation and development of NSPs as novel plant carbohydrate resources.
Article
Biochemistry & Molecular Biology
Fei Shen, Qianqian Wang, Sami Ullah, Ya Pan, Minjie Zhao, Jing Wang, Ming Chen, Fengqin Feng, Hao Zhong
Summary: This study investigated the peristalsis-promoting potential of Ligilactobacillus acidipiscis YJ5 and its underlying molecular mechanism in relieving constipation. The results showed that L. acidipiscis YJ5 intervention increased intestinal peristalsis in zebrafish and mouse models, reduced colonic aquaporin expression, repaired intestinal morphological damage, enhanced the colonic mucosal barrier, and reversed the gut microbiota structure. The positive effects of L. acidipiscis YJ5 were attributed to its metabolites such as malic acid and heliangin.
Article
Biochemistry & Molecular Biology
Zhuo Qu, Jiahui Tian, Jiachen Sun, Ying Shi, Jianqiang Yu, Wannian Zhang, Chunlin Zhuang
Summary: Smoking is the main risk factor for lung cancer, but garlic oil compound DATS shows potential as a chemopreventive agent. DATS can inhibit the carcinogenic effects of tobacco and regulate gut microbiota, as well as activate the PPAR-gamma pathway to suppress tumor growth.
Article
Biochemistry & Molecular Biology
Yanli Zhang, Jielun Hu, Yadong Zhong, Shuai Liu, Liandi Liu, Xinyi Mu, Chunhua Chen, Shenji Yang, Guohao Li, Duoduo Zhang, Xinru Huang, Jinrui Yang, Xiaojun Huang, Shuigen Bian, Shaoping Nie
Summary: The study found that different ratios of insoluble and soluble dietary fiber have distinct effects on gut microbiota and serum metabolites. Increasing the ratio of insoluble dietary fiber increases the relative abundance and acid production of specific bacterial genera, while increasing the ratio of soluble dietary fiber increases the diversity and relative abundance of other bacterial genera and regulates metabolic pathways.
Article
Biochemistry & Molecular Biology
Enhui Tang, Tong Hu, Zhaokang Jiang, Xiaojun Shen, Huan Lin, Haiyan Xian, Xinlan Wu
Summary: Isoquercitrin alleviates lipopolysaccharide-induced intestinal mucosal barrier damage in mice by inhibiting the TLR4/MyD88/NF-kappa B signaling pathway and modulating the intestinal flora.
Article
Biochemistry & Molecular Biology
Nesma A. Abd Elrazik, Sahar A. Helmy
Summary: This study found that betanin, the major pigment in red beetroot, has a protective effect against bleomycin-induced pulmonary fibrosis in rats. The protective effect may be attributed to the attenuation of the NLRP3/IL-1 beta/TGF-beta 1 signaling pathway and the suppression of the EMT process.
Article
Biochemistry & Molecular Biology
Sha Li, Longchen Shang, Yuanyuan Chen, Rong Song, Jing Li, Bin Li
Summary: A new form of konjac fiber was prepared that could instantly expand in contact with digestive fluid, inhibiting the digestion of food. The digestion regulation ability of the fiber was influenced by the freezing temperature used during preparation, with the fiber prepared at -20 degrees C showing the best performance in delaying gastric emptying and preventing intestinal starch hydrolysis.
Article
Biochemistry & Molecular Biology
Xian Xia, Jiangdong Li, Rui Liang, Yi Li, Xiaojuan Ma, Ying Yang, Daniel Lozano-Ojalvo
Summary: This study aimed to reduce the allergenicity of ovomucoid through unfolding-assisted glycation. The results showed that maltose glycation effectively reduced the IgG-binding capacity of the allergen. Unfolded and glycated ovomucoid exhibited enhanced antioxidant activity and metal ion chelating ability.
