Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41467-018-04286-6
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [17H06025, 17H06309, 16H01492, 18H05059, 16H01624, 15H05873, 15H02350, 26250009, 17H04982, 17K13277, 16K18370]
- AMED [JP17dm0207050, JP16dm0207046, JP17dm0107051, JP17dm0207001, JP17dm0107053, JP17dm0207027, JP17dm0107150]
- Nakajima Foundation
- Konica Minolta Science and Technology Foundation
- Tokyo Society of Medical Sciences
- Grants-in-Aid for Scientific Research [15H02350, 17K13277, 17H06309, 16K18370, 16H01492, 26250009, 17H06025, 17H04982, 16H01624, 18H05059] Funding Source: KAKEN
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Two-photon imaging in behaving animals has revealed neuronal activities related to behavioral and cognitive function at single-cell resolution. However, marmosets have posed a challenge due to limited success in training on motor tasks. Here we report the development of protocols to train head-fixed common marmosets to perform upper-limb movement tasks and simultaneously perform two-photon imaging. After 2-5 months of training sessions, head-fixed marmosets can control a manipulandum to move a cursor to a target on a screen. We conduct two-photon calcium imaging of layer 2/3 neurons in the motor cortex during this motor task performance, and detect task-relevant activity from multiple neurons at cellular and subcellular resolutions. In a two-target reaching task, some neurons show direction-selective activity over the training days. In a short-term force-field adaptation task, some neurons change their activity when the force field is on. Two-photon calcium imaging in behaving marmosets may become a fundamental technique for determining the spatial organization of the cortical dynamics underlying action and cognition.
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