Article
Cell Biology
Wenbo Dai, Yifu He, Kai Li
Summary: AS fibroblasts exhibited enhanced osteogenic differentiation potentials, with elevated expression of SPI1. Silencing of SPI1 inhibited osteogenic differentiation, while overexpression of TLR5 reversed this inhibition via NF-κB signaling. SPI1 regulated AS progression by modulating TLR5 through NF-κB signaling.
Article
Biochemistry & Molecular Biology
Ainsley Mike Antao, Kamini Kaushal, Soumyadip Das, Vijai Singh, Bharathi Suresh, Kye-Seong Kim, Suresh Ramakrishna
Summary: USP48 interacts with and stabilizes the Aurora B protein, regulating its levels during the cell cycle. Knockout of USP48 leads to accumulation of mitotic defects and cytokinesis failure, delaying cell cycle progression. This study highlights the important role of USP48 in cell cycle regulation and maintenance of genomic stability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Xinyu Mei, Yuan Guo, Zhangdan Xie, Yedan Zhong, Xiaofen Wu, Daichao Xu, Ying Li, Nan Liu, Zheng-Jiang Zhu
Summary: RIPK1 serves as a critical metabolic regulator that affects neonatal survival under severe starvation conditions, by modulating aspartate levels and autophagy.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yukihiro Sera, Tsuneo Imanaka, Masafumi Yamaguchi
Summary: SDS is a genetic disorder caused by mutations in the SBDS gene, which is involved in ribosome biogenesis. The interaction between SBDS and RNF2 at different stages of the cell cycle may play a role in mitotic progression.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
An-da Shi, Li-ming Zhao, Guo-li Sheng, Ge-ning Zhang, Yong-chang Tang, Kang-shuai Li, Zong-li Zhang
Summary: SMAD4 is downregulated in cholangiocarcinoma (CCA) and is involved in the regulation of tumour immune escape. The correlation between SMAD4 and STING1 and their role in CCA progression were investigated. SMAD4 overexpression suppressed CCA cell migration, invasion, and proliferation, while knockdown enhanced these abilities. Low expression of STING1 and SMAD4 indicated poor prognosis and simultaneous low expression predicted even poorer survival. SMAD4 regulates STING1 expression through transcriptional regulation and dual low expression has a stronger predictive power for patient survival.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Fiorella Faienza, Federica Polverino, Girish Rajendraprasad, Giacomo Milletti, Zehan Hu, Barbara Colella, Deborah Gargano, Flavie Strappazzon, Salvatore Rizza, Mette Vixo Vistesen, Yonglun Luo, Manuela Antonioli, Valentina Cianfanelli, Caterina Ferraina, Gian Maria Fimia, Giuseppe Filomeni, Daniela De Zio, Joern Dengjel, Marin Barisic, Giulia Guarguaglini, Sabrina Di Bartolomeo, Francesco Cecconi
Summary: AMBRA1 is a key factor for nervous system development, primarily associated with autophagy and cell proliferation control. This study reveals that AMBRA1 is phosphorylated during mitosis and is critical for spindle function and orientation, driven by NUMA1 protein. The localization and dynamics of NUMA1 are dependent on AMBRA1 presence, phosphorylation, and binding ability. These findings suggest an additional role of AMBRA1 in tissue morphogenesis and differentiation, which could have implications for development and cancer oncogenesis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Shrividya Sana, Ashwathi Rajeevan, Sachin Kotak
Summary: The exclusive localization of NuMA and Ect2/Cyk4/Mklp1 ensures the coordination of spindle elongation and cleavage furrow formation. This coordination is achieved by restricting dynein/dynactin and RhoA to distinct membrane zones.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hyunmi Kim, Da Som Lee, Tae Hyeon An, Tae-Jun Park, Eun-Woo Lee, Baek Soo Han, Won Kon Kim, Chul-Ho Lee, Sang Chul Lee, Kyoung-Jin Oh, Kwang-Hee Bae
Summary: GADD45 beta plays a crucial role in hepatic gluconeogenesis, with its overexpression increasing glucose output and deficiency leading to downregulation of gluconeogenic gene expression. GADD45 beta regulates FoxO1 transcriptional activity by enhancing its protein stability, thus controlling hepatic gluconeogenesis.
Article
Chemistry, Multidisciplinary
Qizhi Geng, Yue-Yu Kong, Weizhe Li, Jianhao Zhang, Haoli Ma, Yuhang Zhang, Lin-Tai Da, Yan Zhao, Hai-Ning Du
Summary: This study uncovers that, during mitosis, the kinase Plk1 and the phosphatase PPP2CB dynamically phosphorylates/dephosphorylates the methyltransferase G9a to regulate H3K9me2 levels, chromatin accessibility, and mitotic progression, which highlights the importance of the crosstalk in chromatin organization and mitotic events.
Article
Biochemistry & Molecular Biology
Kai Li, Shuyang Sun, Yixun Lu, Wenquan Liang, Xinxin Xu, Huan Zhang, Zhengyao Chang, Chuang Wang, Yunhe Gao, Lin Chen
Summary: In this study, the expression and function of MT1M in gastric cancer (GC) were investigated. It was found that MT1M expression was significantly downregulated in GC tissues and cell lines. Decreased expression of MT1M was associated with worse clinical prognosis, and overexpression of MT1M inhibited cell proliferation and induced apoptosis in GC cells. Furthermore, MT1M overexpression could inhibit stem cell characteristics by targeting GLI1 and affecting GLI1 ubiquitination.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Zhihao Yuan, Guancheng Guo, Guifang Sun, Qi Li, Lihui Wang, Baoping Qiao
Summary: This study found that Magnesium isoglycyrrhizinate (MI) has significant anti-tumor effects against bladder cancer, inhibiting cell proliferation, invasion, migration, and inducing apoptosis. MI increases the expression of miR-26b and Nox4 is identified as its direct target. Furthermore, MI downregulates the expression of Nox4, NF-kappa B, and HIF-1 alpha, exerting its anti-tumor effects through modulating the miR-26b/Nox4 axis.
Article
Biochemistry & Molecular Biology
Hengqing Zhu, Qing Li, Yulan Zhao, Hong Peng, Liangyun Guo, Jing Zhu, Zi Jiang, Zhaoxia Zeng, Bin Xu, Sisi Chen
Summary: The study revealed the mechanism of overexpression of Plk1 in pancreatic cancer (PC) and explored the potential therapeutic strategy of targeting vaccinia-related kinase 2 (VRK2) as a treatment target.
Article
Endocrinology & Metabolism
Xuan Shen, Yajun Zhang, Xuetao Ji, Bo Li, Yuzhu Wang, Yun Huang, Xu Zhang, Jingxian Yu, Ruihan Zou, Dongdong Qin, Hongwen Zhou, Qian Wang, John Zhong Li
Summary: A novel lncRNA regulator of hyperlipidemia (lncRHL) was identified in this study, showing differential expression in the liver under high-fat diet and oleic acid treatment, and regulating hepatic VLDL secretion. This lncRNA maintains intrahepatic and plasma lipid homeostasis by modulating the hnRNPU/BMAL1/MTTP axis.
Article
Chemistry, Physical
Qianqian Wang, Qinggao Zhang, Elaine Lai Han Leung, Yingqing Chen, Xiaojun Yao
Summary: This study applied integrated molecular dynamics techniques to investigate the characteristics of 5-iTU and its derivatives targeting haspin, revealing the crucial role of 5-iTU in binding with haspin and the importance of gatekeeper Phe605.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Luis Cedeno-Rosario, David Honda, Autumn M. Sunderland, Mark D. Lewandowski, William R. Taylor, Deborah N. Chadee
Summary: MLK3 plays a crucial role in the cell cycle of ovarian cancer cells, with its activity being regulated by phosphorylation by CDK1 and CDK2, affecting the activities of both MLK3 and c-Jun N-terminal kinase in G1/S, G2, and M phases.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Oncology
Jinhong Cho, Jinyoung Park, Sang Chul Shin, Mihue Jang, Jae-Hong Kim, Eunice EunKyeong Kim, Eun Joo Song
Review
Biochemistry & Molecular Biology
Tanuza Das, Sang Chul Shin, Eun Joo Song, Eunice EunKyeong Kim
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Review
Biochemistry & Molecular Biology
Jinhong Cho, Jinyoung Park, Eunice EunKyeong Kim, Eun Joo Song
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Jiho Park, Song Y. Yeu, Sangjin Paik, Hyungmin Kim, Si-Young Choi, Junyeop Lee, Jinho Jang, Semin Lee, Youngil Koh, Hyunsook Lee
Summary: Loss of BubR1 acetylation leads to chromosomal rearrangement and genetic instability in mice, with replication stress playing a key role in these processes. Moreover, defects in BubR1 acetylation in mitosis contribute to tumorigenesis, as observed in human cancer cells with whole-arm translocations demonstrating similar defects.
Review
Biochemistry & Molecular Biology
Tanuza Das, Eun Joo Song, Eunice EunKyeong Kim
Summary: This review discusses the important role of deubiquitinase USP15 in various signaling pathways, highlighting its mechanisms in regulating protein stability and signal transduction processes. Understanding the regulatory mechanisms of USP15 can provide important insights into possible disease markers and clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Eunmi Ban, Haejin Kwon, Eun Joo Song
Summary: A sensitive and specific capillary electrophoresis with laser-induced fluorescence (CE-LIF) method was developed for simultaneous detection of G3139 and its metabolites, with evaluation of pharmacokinetics in rat plasma. The method showed excellent linearity, detection limit, and recovery rate, allowing for effective analysis and providing valuable information for ASOs therapeutic research.
NUCLEIC ACID THERAPEUTICS
(2021)
Article
Biotechnology & Applied Microbiology
Eunmi Ban, Haejin Kwon, Hong Seog Seo, Young Sook Yoo, Eun Joo Song
Summary: This study successfully optimized the conditions for extraction and qRT-PCR, improving miRNA yields while minimizing interference and loss. The research aimed to achieve reliable results by reducing contaminants and maximizing product yields for miRNA analysis in plasma samples.
Review
Neurosciences
Seung Jae Hyeon, Jinyoung Park, Junsang Yoo, Su-Hyun Kim, Yu Jin Hwang, Seung-Chan Kim, Tian Liu, Hyun Soo Shim, Yunha Kim, Yakdol Cho, Jiwan Woo, Key-Sun Kim, Richard H. Myers, Hannah L. Ryu, Neil W. Kowall, Eun Joo Song, Eun Mi Hwang, Hyemyung Seo, Junghee Lee, Hoon Ryu
Summary: Mitochondrial dysfunction in Huntington's disease is associated with a decrease in XIAP-p53 colocalization and a reduction in XIAP levels due to mutant Huntingtin overexpression, leading to mitochondrial localization of p53. XIAP interacts with p53 C-terminal domain and decreases its stability via autophagy, preventing mitochondrial oxidative stress and cell death. Dysregulation of XIAP triggers mitochondrial dysfunction and neuropathological processes, while XIAP overexpression improves neuropathology in mouse models of HD.
PROGRESS IN NEUROBIOLOGY
(2021)
Correction
Multidisciplinary Sciences
Manivannan Subramanian, Seung Jae Hyeon, Tanuza Das, Yoon Seok Suh, Yun Kyung Kim, Jeong-Soo Lee, Eun Joo Song, Hoon Ryu, Kweon Yu
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Manivannan Subramanian, Seung Jae Hyeon, Tanuza Das, Yoon Seok Suh, Yun Kyung Kim, Jeong-Soo Lee, Eun Joo Song, Hoon Ryu, Kweon Yu
Summary: The study identifies the miR-9 family as suppressors of Tau-mediated neurodegeneration in Alzheimer's disease. Through experiments in Drosophila and mammalian cells, it is revealed that UBE4B and STUB1 promote autophagic degradation of Tau through ubiquitination, suggesting a potential therapeutic target for AD.
NATURE COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Eunmi Ban, Eun Joo Song
Summary: MicroRNAs (miRNAs) play an important role in the early diagnosis, targeted therapy, and prognosis of diseases, and their detection in plasma or serum is useful in the discovery of biomarkers and disease diagnosis. However, the application of quantitative reverse-transcription polymerase chain reaction (qRT-PCR) in miRNA analysis is limited by contaminants. This review highlights the factors influencing the qualification and quantification of miRNAs in plasma and the importance of evaluating extraction and normalization methods for reliable miRNA analysis.
Article
Oncology
Jinyoung Park, Moonsoo Cho, Jinhong Cho, Eunice EunKyeong Kim, Eun Joo Song
Summary: Abnormal expression of microRNA is common in cancer, and it is important to understand the exact mechanism for the regulation of cancer growth by microRNAs. In this study, we demonstrate that miR-101-3p, which is downregulated in several cancers, regulates the RPL11-MDM2-p53 pathway by targeting USP47, thereby inhibiting cancer cell proliferation. Our findings suggest that miR-101-3p functions as a tumor suppressor by targeting USP47 and could be a potential therapeutic target for cancers.
Article
Cell Biology
Tanuza Das, Eun-Young Lee, Hye Jin You, Eunice EunKyeong Kim, Eun Joo Song
Summary: The deubiquitinating enzyme USP15 and its close paralog USP4 regulate the alternative splicing of SRSF1, promoting lung cancer cell proliferation. The depletion of USP15 and USP4 leads to impairment of SRSF1 splicing, resulting in an alternative isoform SRSF1-3. The increased expression of USP15 contributes to the development of lung adenocarcinoma and is associated with lower disease-specific survival in patients.
CELL DEATH DISCOVERY
(2022)
Article
Multidisciplinary Sciences
Junyeop Lee, Keewon Sung, So Young Joo, Jun-Hyeon Jeong, Seong Keun Kim, Hyunsook Lee
Summary: The dynamic interaction between BRCA2 and telomeric G-quadruplex (G4) plays a crucial role in telomere replication. Binding of BRCA2 remodels telomeric G4 structure, allowing the restart of stalled replication forks driven by G4 and preventing telomere breakdown.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Medicinal
Jinyoung Park, Jinhong Cho, Eun Joo Song
ARCHIVES OF PHARMACAL RESEARCH
(2020)
