Journal
JOURNAL OF PLANT INTERACTIONS
Volume 13, Issue 1, Pages 213-220Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/17429145.2018.1471529
Keywords
MdNAS1; iron; strategy I plant; Malus domestica; transgenic tobacco; abnormal flower
Categories
Funding
- National Natural Science Foundation of China [31301757]
- Natural Science Foundation of Heilongjiang Province of China [C2015015]
- Academic Backbone Project of Northeast Agricultural University [15XG06]
- Postdoctoral Scientific Research Development Fund of Heilongjiang Province, China [LBH-Q16020]
Ask authors/readers for more resources
Metal elements are essential micronutrients required by all plants for natural physiological activities. Nicotianamine is considered as the chelate substance in the transport of metal ions. In the present study, a new gene encoding NA synthase was isolated from Malus domestica (L.) Borkh and designated as MdNAS1. The expression levels of MdNAS1 were enriched in leaf, and phloem which were highly affected by Fe stress, indoleacetic acid (IAA) and abscisic acid (ABA) treatments in M.domestica seedlings. Subcellular localization research revealed that MdNAS1 was localized in cytoplasmic membrane. Overexpression of MdNAS1 in transgenic tobaccos increased the tolerance to Fe stress, but also contributes to higher chlorophyll, NA, Fe, Mn, Cu and Zn contents and abnormal flowers. Moreover, the MdNAS1-OE tobaccos had the increased expression levels of Fe uptake and transport related genes (NtFRO, NtIRT1, NtVIT, NtNRAMP1, and NtYSL).
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available