Article
Biophysics
Min Qing, Yi Fan, Sheng Liang Chen, Hong Qun Luo, Nian Bing Li
Summary: The study developed a novel microelectrode for detecting DNA methyltransferase activity, which features rapid response time, high sensitivity, and reusability, and can be applied to research on genetic disorders and tumors.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Danxia Song, Deyu Yuan, Xuemei Tan, Ling Li, Huan He, Liang Zhao, Gang Yang, Sirui Pan, Hongyuan Dai, Xu Song, Yongyun Zhao
Summary: A label-free aptamer-based amplification assay, called the ATC-TA system, that can detect very low concentrations of proteins without time-consuming washing steps and pre-treatment, has been developed. The system exhibits ultrahigh sensitivity and comparable accuracy to conventional ELISA, and has potential application in early clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Qian Zhao, Guangyin Zhang, Dai Lu, Kejun Feng, Xingbo Shi
Summary: A highly sensitive aptasensor based on dual-enzyme mediated cascade-signal amplification was developed for the detection of ampicillin. The sensor exhibited excellent detection performance and versatility, paving the way for efficient sensors for detecting other trace food hazards.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Haobo Sun, Lisheng Qian, Jinming Kong, Xueji Zhang
Summary: A novel electrochemical biosensor platform with triple helix structure switch and double signal amplification strategy was proposed for nucleic acid detection, achieving ultra-sensitivity and high selectivity. The biosensor showed a detection limit as low as 1.954 aM and a linear range of 7 orders of magnitude. Additionally, it demonstrated excellent anti-interference ability when analyzing DNA in serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Xiaofan Zhang, Shunjun Xie, Xue Chen, Li Wang, Fang Li, Shufeng Liu
Summary: A new catalytic DNA circuit reaction strategy has been proposed for sensitive nucleic acid detection, utilizing DNA switch-mediated reactions to achieve signal amplification and ratiometric signal response mode, enabling detection limits as low as 0.1 pM and selective discrimination towards different mismatched targets.
ANALYTICAL METHODS
(2022)
Article
Biophysics
Jun-Tao Cao, Yi-Zhuo Fu, Yu-Ling Wang, Hong-Ding Zhang, Xiang-Mei Liu, Shu-Wei Ren, Yan-Ming Liu
Summary: This study presents a novel signal amplification strategy for electrochemiluminescence (ECL) biosensors based on liposome-assisted chemical redox cycling. The strategy involves the in situ formation of gold nanoparticles (Au NPs) on titanium dioxide nanotubes (TiO2 NTs) electrode. The detection of prostate specific antigen (PSA) was demonstrated using ascorbic acid (AA)-loaded liposome and Au nanoclusters (Au NCs)/TiO2 NTs as working electrodes. The multiple signal amplification strategy achieved ultrasensitive detection of PSA with a wide linear concentration range.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yuqi Huang, Wenxiu Zhang, Shuhui Zhao, Zuowei Xie, Siyi Chen, Gang Yi
Summary: DNA methylation is crucial in genetics, and a novel fluorescent biosensor was developed for detecting DNA methyltransferase. The method showed good sensitivity, specificity, and applicability for early cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Tatjana Safarik, Aleksandar Karajic, Stephane Reculusa, Philip N. Bartlett, Nicolas Mano, Alexander Kuhn
Summary: This study reports the electrochemical elaboration and characterization of a 3D coaxial macroporous twin-electrode, which is obtained by a low-cost bottom-up approach using nanoengineered highly organized porous material. The resulting electrode architecture allows for signal amplification in electroanalytical measurements and has potential applications in various (bio)electroanalytical fields.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Guanli Fan, Xia Li, Shuling Xu, Caifeng Dai, Qingwang Xue, Huaisheng Wang
Summary: A novel strategy based on DNA-biobarcode and SERS technology was developed for sensitive detection of telomerase activity. The strategy achieved measurement of telomerase activity down to 3 HeLa cells through copper-mediated signal amplification. It was successfully applied for analysis of telomerase activities in different cell lines and screening of telomerase inhibitors.
Article
Engineering, Environmental
Feng Hong, Rui Chen, Peng Lu, Letian Li, Ruiheng Xiao, Yiping Chen, Hong Yang
Summary: Inspired by the stability and biocompatibility of polydopamine, a universal, portable, and ultra-sensitive pipet immunoassay platform was developed for the detection of the highly toxic mycotoxin deoxynivalenol (DON). The platform showed high sensitivity, reduced detection cost, and simplified experimental steps.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Analytical
Yayun Yang, Xiaoting Liu, Nan Zhang, Wei Jiang
Summary: In this study, a dumbbell probe-based triple cascade signal amplification strategy was proposed for sensitive and specific detection of UDG activity. The method significantly improved sensitivity, ensured specificity, and could also be used for inhibitor screening.
Article
Chemistry, Multidisciplinary
Jintao Yi, Xianqin Han, Fengying Gao, Le Cai, Ying Chen, Xiulong Deng, Xun Li, Jun Xue, Hui Zhou
Summary: Researchers have developed a novel Ba-hemin@GOX biosensor for glucose detection using a simple biomineralization method, and have verified its operability and accuracy in real samples. The sensor exhibits a low detection limit, good selectivity, storage stability, and can be reused multiple times.
Article
Chemistry, Physical
Andrey N. Pravdivtsev, Kai Buckenmaier, Nicolas Kempf, Gabriele Stevanato, Klaus Scheffler, Joern Engelmann, Markus Plaumann, Rainer Koerber, Jan-Bernd Hoevener, Thomas Theis
Summary: Nuclear spin hyperpolarization allows for real-time observation of metabolism and intermolecular interactions in vivo. The use of 1-13C-pyruvate as a hyperpolarized tracer is being evaluated in clinical trials and shows promise as a molecular imaging agent. However, the search for a simple, fast, and efficient hyperpolarization technique is ongoing.
JOURNAL OF PHYSICAL CHEMISTRY C
(2023)
Article
Pharmacology & Pharmacy
Jun Xu, Qing Tang, Runhui Zhang, Haoyi Chen, Bee Luan Khoo, Xinguo Zhang, Yue Chen, Hong Yan, Jincheng Li, Huaze Shao, Lihong Liu
Summary: The study presents the development of a sensitive and efficient biosensor for miRNA analysis using polyadenine-mediated fluorescent spherical nucleic acid. The method exhibits high selectivity and sensitivity and can be used for the detection of miRNA-21 in human samples.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Guoliang Zheng, Jiangshan Dai, Haiyan Wang, Ling Li, Deyu Yuan, Shujun Bai, Xu Song, Yongyun Zhao
Summary: HNESA is a novel signal amplification technique for nucleic acid detection which overcomes the limitations of traditional methods, demonstrates high sensitivity, can distinguish mismatched sequences, and has potential applications in complex biological samples.
Article
Chemistry, Multidisciplinary
Alexander M. Prophet, Kritanjan Polley, Gary J. Van Berkel, David T. Limmer, Kevin R. Wilson
Summary: The oxidation kinetics of iodide by ozone at the air-water interface is studied in single microdroplets. Molecular simulations and kinetic modeling are used to understand the underlying multiphase mechanism.
Article
Chemistry, Multidisciplinary
Rong Zhang, Jiajing Lan, Fei Wang, Shumei Chen, Jian Zhang
Summary: By utilizing 1,1'-ferrocene dicarboxylic acid as a chelating and surface protection ligand, we have synthesized multi-nuclear indium oxide clusters with varying nuclear sizes, including heptanuclear and thirteen-nuclear clusters. These clusters possess labile coordination sites, allowing for structural modification and self-assembly, resulting in the formation of various cluster structures.
Article
Chemistry, Multidisciplinary
Hui Wang, Hui Liu, Mingsen Wang, Jiaheng Hou, Yongjun Li, Yuancheng Wang, Yingjie Zhao
Summary: Two supramolecular complexes were prepared using CBs and M1, and their single-crystal structures were analyzed by SCXRD. The unexpected 1:2 self-assembly structure between M1 and CB[8] was discovered for the first time. These complexes exhibit unique photophysical properties and provide valuable information about the structure and photophysical properties of supramolecular complexes.
Article
Chemistry, Multidisciplinary
Chang-Hee Lee, Sookil Park, Sanggil Kim, Ji Young Hyun, Hyun Soo Lee, Injae Shin
Summary: The epidermal growth factor receptor (EGFR) is a cell-surface glycoprotein involved in cell proliferation and tumor development. This study used a fluorescently labeled EGFR to investigate its time-dependent endocytosis in live cells and found that appended glycans affect EGFR internalization. Additionally, the study detected sialic acid residues attached to EGFR on the live cell surface using FRET-based imaging. This research provides valuable insights into the cellular functions of EGFR.
Article
Chemistry, Multidisciplinary
Subhankar Sahu, Lokesh Kumar, Sumita Das, Dipti Gupta, Ruchi Anand
Summary: This study proposes a strategy that combines organic electronics with biosensor scaffolds to create a compact device for monitoring environmental aromatic pollution. By coupling biosensing protein MopR with an organic electrochemical transistor (OECT), a sensor module capable of efficient detection of phenol was designed. Exclusive phenol detection with minimal loss of sensitivity could be achieved in complex pollutant mixtures and real environmental samples.
Review
Chemistry, Multidisciplinary
Changseok Lee, Hyung-Joon Kang, Sungwoo Hong
Summary: The formation of C-N bonds through hydroamination reactions catalyzed by nickel hydrides has been a topic of recent interest. This approach offers a way to efficiently transform a variety of alkene and alkyne substrates into compounds enriched with C-N bonds. The review provides a concise overview of the underlying reaction mechanisms and aims to stimulate further progress in NiH-catalytic techniques and catalyst design.
Article
Chemistry, Multidisciplinary
Yueci Wu, Lu-Lu Sun, Hai-Hao Han, Xiao-Peng He, Weiguo Cao, Tony D. James
Summary: Drug-induced liver injury (DILI) is a common cause of acute liver failure in the USA and Europe, but most cases can be recovered or prevented by discontinuing the offending drug. Recent research has found that peroxynitrite (ONOO-) can be used as a potential indicator for early diagnosis of DILI, and there is an urgent need to establish a method to detect and track peroxynitrite in DILI cases. In this study, a FRET-based nano fluorescent probe CD-N-I was developed, which showed high selectivity and sensitivity in detecting peroxynitrite. The probe successfully detected exogenous peroxynitrite in live cells and endogenous peroxynitrite in APAP-induced liver injury of HepG2 cells.
Article
Chemistry, Multidisciplinary
Dmitry L. Lipilin, Mikhail O. Zubkov, Mikhail D. Kosobokov, Alexander D. Dilman
Summary: This article describes a direct photocatalytic method for the thiolation of unprotected acids, which was previously challenging. By using a thionocarbonate reagent with an N-O bond, the efficient conversion of carboxylic acids to thiols is achieved.
Article
Chemistry, Multidisciplinary
Jason Malenfant, Lucille Kuster, Yohann Gagne, Kouassi Signo, Maxime Denis, Sylvain Canesi, Mathieu Frenette
Summary: Raman microscopy can reveal compound-specific vibrational fingerprints without sample preparation. The combination of efficient theoretical calculations and a user-friendly software can accurately predict peak positions and provide match scores to assist with structure determination.
Article
Chemistry, Multidisciplinary
Jayoh A. Hernandez, Paul S. Micus, Sean Alec Lois Sunga, Luca Mazzei, Stefano Ciurli, Gabriele Meloni
Summary: Essential trace metals play crucial roles in the survival and virulence of bacterial pathogens. Helicobacter pylori requires nickel for colonization and persistence in the stomach, and NixA is an essential nickel transporter in this process. This study characterizes the selectivity and electrogenic nature of NixA-mediated nickel transport.
Article
Chemistry, Multidisciplinary
Tarali Devi, Kuheli Dutta, Jennifer Deutscher, Stefan Mebs, Uwe Kuhlmann, Michael Haumann, Beatrice Cula, Holger Dau, Peter Hildebrandt, Kallol Ray
Summary: This study emphasizes the importance of subtle electronic changes and secondary interactions in the stability of biologically relevant metal-dioxygen intermediates. It also shows that the role of the chloride ligand in stabilizing the Fe-III-(OOBu)-Bu-t moiety can extend to other anions, including the thiolate ligand.
Article
Chemistry, Multidisciplinary
Jacqueline R. Santhouse, Jeremy M. G. Leung, Lillian T. Chong, W. Seth Horne
Summary: By studying the folding kinetics and mechanism of the BdpA sequence, researchers found that altering the backbone connectivity can affect protein folding. This suggests that protein mimetic chains have a significant degree of plasticity in transitioning between unfolded and folded states.
Article
Chemistry, Multidisciplinary
Divanshu Gupta, Ralf Einholz, Holger F. Bettinger
Summary: This study presents the first direct spectroscopic evidence of a cyclic seven-membered iminoborane. Compared to linear amino-iminoboranes, this cyclic iminoborane exhibits weakened bond strength and lower Lewis acidity value. The study suggests that the reduced ring strain of cyclic iminoborane prevents nitrogen fixation but allows facile (2 + 2) cycloaddition reaction with C2H4.
Article
Chemistry, Multidisciplinary
Renny Mathew, Aniruddha Mazumder, Praveen Kumar, Julie Matula, Sharmarke Mohamed, Petr Brazda, Mahesh Hariharan, Brijith Thomas
Summary: This study reveals the packing arrangement of partially disordered nitro-perylenediimide (NO2-PDI) using a synergistic approach that combines 3D ED, ssNMR, and DFT techniques. By overcoming these challenges, this methodology opens up new avenues for material characterization, driving exciting advancements in the field.