History of genome editing in yeast

A synthetic construct for genetic engineering of the emerging pathogenic yeast Candida auris

(2018) Tatiana A. Defosse et al.   PLASMID

Systematic testing of enzyme perturbation sensitivities via graded dCas9 modulation in Saccharomyces cerevisiae

(2017) Matthew Deaner et al.   METABOLIC ENGINEERING

A resource for functional profiling of noncoding RNA in the yeast Saccharomyces cerevisiae

(2017) Steven Parker et al.   RNA

Beyond Native Cas9: Manipulating Genomic Information and Function

(2017) Hitoshi Mitsunobu et al.   TRENDS IN BIOTECHNOLOGY

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

(2016) Astrid Weninger et al.   JOURNAL OF BIOTECHNOLOGY

Multiplex gene editing of the Yarrowia lipolytica genome using the CRISPR-Cas9 system

(2016) Shuliang Gao et al.   JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae

(2016) Wesley Cardoso Generoso et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Widespread Impact of Chromosomal Inversions on Gene Expression Uncovers Robustness via Phenotypic Buffering

(2016) Samina Naseeb et al.   MOLECULAR BIOLOGY AND EVOLUTION

Genome engineering in the yeast pathogen Candida glabrata using the CRISPR-Cas9 system

(2016) Ludovic Enkler et al.   Scientific Reports

Synthetic RNA Polymerase III Promoters Facilitate High-Efficiency CRISPR–Cas9-Mediated Genome Editing in Yarrowia lipolytica

(2016) Cory M. Schwartz et al.   ACS Synthetic Biology

Targeted Genome Editing via CRISPR in the Pathogen Cryptococcus neoformans

(2016) Samantha D. M. Arras et al.   PLoS One

A ‘suicide’ CRISPR-Cas9 system to promote gene deletion and restoration by electroporation in Cryptococcus neoformans

(2016) Yu Wang et al.   Scientific Reports

CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae

(2015) Robert Mans et al.   FEMS YEAST RESEARCH

SCRaMbLE generates designed combinatorial stochastic diversity in synthetic chromosomes

(2015) Yue Shen et al.   GENOME RESEARCH

Multiplex metabolic pathway engineering using CRISPR/Cas9 in Saccharomyces cerevisiae

(2015) Tadas Jakočiūnas et al.   METABOLIC ENGINEERING

Single-Molecule Imaging Reveals a Switch between Spurious and Functional ncRNA Transcription

(2015) Tineke L. Lenstra et al.   MOLECULAR CELL

Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas

(2015) Andrew A. Horwitz et al.   Cell Systems

A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families

(2015) V. K. Vyas et al.   Science Advances

Construction of a Quadruple Auxotrophic Mutant of an Industrial Polyploid Saccharomyces cerevisiae Strain by Using RNA-Guided Cas9 Nuclease

(2014) Guo-Chang Zhang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Total Synthesis of a Functional Designer Eukaryotic Chromosome

(2014) N. Annaluru et al.   SCIENCE

Yarrowia lipolyticaand Its Multiple Applications in the Biotechnological Industry

(2014) F. A. G. Gonçalves et al.   TheScientificWorldJOURNAL

Implementation of the CRISPR-Cas9 system in fission yeast

(2014) Jake Z. Jacobs et al.   Nature Communications

Homology-Integrated CRISPR–Cas (HI-CRISPR) System for One-Step Multigene Disruption in Saccharomyces cerevisiae

(2014) Zehua Bao et al.   ACS Synthetic Biology

Selection of chromosomal DNA libraries using a multiplex CRISPR system

(2014) Owen W Ryan et al.   eLife

CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes

(2013) Luke A. Gilbert et al.   CELL

Increased homologous integration frequency in Yarrowia lipolytica strains defective in non-homologous end-joining

(2013) Anne Kretzschmar et al.   CURRENT GENETICS

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems

(2013) James E. DiCarlo et al.   NUCLEIC ACIDS RESEARCH

Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas

(2013) Fahim Farzadfard et al.   ACS Synthetic Biology

Deletion of the Pichia pastoris KU70 Homologue Facilitates Platform Strain Generation for Gene Expression and Synthetic Biology

(2012) Laura Näätsaari et al.   PLoS One

Impact of Chromosomal Inversions on the Yeast DAL Cluster

(2012) Samina Naseeb et al.   PLoS One

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Novel genetic tools for Hansenula polymorpha

(2011) Ruchi Saraya et al.   FEMS YEAST RESEARCH

Synthetic chromosome arms function in yeast and generate phenotypic diversity by design

(2011) Jessica S. Dymond et al.   NATURE

Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe

(2010) Dong-Uk Kim et al.   NATURE BIOTECHNOLOGY

New generation of loxP-mutated deletion cassettes for the genetic manipulation of yeast natural isolates

(2010) Zorana Carter et al.   YEAST

Ku70orKu80deficiencies in the fungusBotrytis cinereafacilitate targeting of genes that are hard to knock out in a wild-type context

(2008) Mathias Choquer et al.   FEMS MICROBIOLOGY LETTERS

Nonhomologous end joining and homologous recombination DNA repair pathways in integration mutagenesis in the xylose-fermenting yeastPichia stipitis

(2008) Nicole Maassen et al.   FEMS YEAST RESEARCH

Overexpression of the riboflavin biosynthetic pathway in Pichia pastoris

(2008) Hans Marx et al.   Microbial Cell Factories