Journal
XENOTRANSPLANTATION
Volume 25, Issue 2, Pages -Publisher
WILEY
DOI: 10.1111/xen.12378
Keywords
insulin secretion; macro-encapsulation; microfluidic system; neonatal pancreatic cell clusters; oxygen-generating scaffold
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Funding
- Ministry of Trade, Industry and Energy of Korea [10048948]
- Korean Government
- Ministry of Science, ICT & Future Planning [2014M3A9D3034034, 2011-0030075]
- Korean Government, MSIP
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BackgroundIslet encapsulation techniques have shown limited success in maintaining islet survival and function because encapsulation decreases oxygen supply. In this study, an oxygen-generating scaffold was fabricated to prevent hypoxic cell damage and improve the viability and insulin secretion of islets. MethodsWe fabricated an oxygen-generating scaffold by mixing calcium peroxide (CaO2) with polydimethylsiloxane (PDMS). We evaluated the effects of the oxygen-generating PDMS+CaO2 scaffold on viability, caspase-3 and caspase-7 activity, oxygen consumption rate (OCR), glucose-stimulated insulin secretion (GSIS), hypoxic cell marker expression, and reactive oxygen species (ROS) levels in porcine neonatal pancreatic cell clusters (NPCCs). We also fabricated a microfluidic device that allowed measuring the effects of the oxygen-generating scaffold on viability. ResultsOxygen generation by the PDMS+CaO2 scaffold was sustained for more than 24hours in vitro. NPCCs encapsulated in PDMS+CaO2 showed higher viability than NPCCs in PDMS scaffolds and control NPCCs grown without a scaffold. PDMS+CaO2-encapsulated NPCCs showed lower caspase-3 and caspase-7 activity, hypoxic cell expression, and ROS levels, and higher OCR and GSIS than those in PDMS or control cells. Using the microfluidic device, we observed that the viability of PDMS+CaO2-encapsulated NPCCs was higher than that of PDMS-encapsulated NPCCs. ConclusionsNPCCs in PDMS+CaO2 scaffolds show higher viability and insulin secretion than do NPCCs in PDMS scaffolds and control cells. Therefore, this oxygen-generating scaffold has potential for application in future islet transplantation studies.
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