Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 34, Issue 5, Pages -Publisher
SPRINGER
DOI: 10.1007/s11274-018-2450-7
Keywords
Bacillus licheniformis; Optical purity; D-2,3-Butanediol; meso-2,3-Butanediol; Miscanthus floridulus
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2,3-Butanediol (2,3-BD) can be produced by fermentation of natural resources like Miscanthus. Bacillus licheniformis mutants, WX-02 Delta budC and WX-02 Delta gldA, were elucidated for the potential to use Miscanthus as a cost-effective biomass to produce optically pure 2,3-BD. Both WX-02 Delta budC and WX-02 Delta gldA could efficiently use xylose as well as mixed sugars of glucose and xylose to produce optically pure 2,3-BD. Batch fermentation of M. floridulus hydrolysate could produce 21.6 g/L d-2,3-BD and 23.9 g/L meso-2,3-BD in flask, and 13.8 g/L d-2,3-BD and 13.2 g/L meso-2,3-BD in bioreactor for WX-02 Delta budC and WX-02 Delta gldA, respectively. Further fed-batch fermentation of hydrolysate in bioreactor showed both of two strains could produce optically pure 2,3-BD, with 32.2 g/L d-2,3-BD for WX-02 Delta budC and 48.5 g/L meso-2,3-BD for WX-02 Delta gldA, respectively. Collectively, WX-02 Delta budC and WX-02 Delta gldA can efficiently produce optically pure 2,3-BD with M. floridulus hydrolysate, and these two strains are candidates for industrial production of optical purity of 2,3-BD with M. floridulus hydrolysate.
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