Article
Immunology
Lydia Verlaat, Katarina Riesner, Martina Kalupa, Beate Jung, Sarah Mertlitz, Constanze Schwarz, Joerg Mengwasser, Claudine Fricke, Olaf Penack
Summary: Despite progress in allo-HCT, cGvHD remains a major hurdle, and improved pre-clinical models are needed. We developed two murine cGvHD models that resemble the clinical manifestations of cGvHD in patients. These models exhibited diverse signs of cGvHD in target organs and decelerated immune cell reconstitution. They can provide a deeper understanding of cGvHD pathology and facilitate the translation of experimental findings into human allo-HCT.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Physiology
Annette Robichaud, Liah Fereydoonzad, Samuel L. Collins, Jeffrey Martin Loube, Yumiko Ishii, Maureen R. Horton, James G. Martin, Wayne Mitzner
Summary: The quantification of airway compliance (C-aw) using the inflation limb of full-range pressure-volume (PV) curves was found to be feasible in three mouse models of respiratory diseases. Changes in C-aw were consistent with other respiratory metrics and results reported in literature, demonstrating the potential utility of direct C-aw measurements in small subjects like mice for characterizing respiratory disease models and treatment effects.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Microbiology
Efthymia Iliana Matthaiou, Wayland Chiu, Carol Conrad, Joe Hsu
Summary: Cystic fibrosis lung transplant recipients are at a higher risk of life-threatening invasive aspergillosis. Alkalization of macrophages lacking the CFTR gene is associated with this increased risk, and the presence of iron in the post-transplant microenvironment exacerbates this alkalization. The alkalization impairs the ability of macrophages to clear Aspergillus fumigatus conidia. pH modulation after transplantation may be a therapeutic strategy to reduce the risk of invasive aspergillosis.
Review
Physiology
Olivia R. Carroll, Amber L. Pillar, Alexandra C. Brown, Min Feng, Hui Chen, Chantal Donovan
Summary: Recent advances in mouse models and respiratory physiology assessments have improved the accuracy and human relevance of asthma studies. These models have become important testing platforms and have accelerated the discovery of asthma pathogenesis. Distinctions in respiratory physiology between asthma and severe asthma, as well as measurement techniques and exposure studies, have increased our understanding of lung physiology and identified new therapeutic targets.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hee Jin Park, Ok-Yi Jeong, Sung Hak Chun, Yun Hong Cheon, Mingyo Kim, Suhee Kim, Sang-Il Lee
Summary: The study found that butyrate has potential therapeutic effects in a mouse model of SSc and human dermal fibroblasts, by modulating intestinal microbiota, controlling macrophage differentiation, and inhibiting proinflammatory gene expression, thus improving fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dermatology
Amos Gilhar, Kristian Reich, Aviad Keren, Kenji Kabashima, Martin Steinhoff, Ralf Paus
Summary: Mouse models for atopic dermatitis have limitations in reflecting the clinical complexity of human AD, and it is suggested that valid models should meet specific criteria to be considered relevant for human AD research. Humanized AD mouse models offer a promising alternative to current mouse models for preclinical research.
EXPERIMENTAL DERMATOLOGY
(2021)
Article
Respiratory System
Mwelwa Chizinga, Tiago N. Machuca, Abbas Shahmohammadi, Divya C. Patel, Ayoub Innabi, Bashar Alzghoul, Vanessa Scheuble, Mauricio Pipkin, Borna Mehrad, Andres Pelaez, Christine Lin, Diana Gomez-Manjarres
Summary: This study found that there was no significant difference in 1-year survival between patients undergoing lung transplantation during AE-ILD and those transplanted with stable disease. Patients with AE-ILD who underwent transplantation had higher survival rates compared to those who were medically treated.
Article
Immunology
Mihaly Balogh, Jixiang Zhang, Caitlyn M. Gaffney, Neha Kalakuntla, Nicholas T. Nguyen, Ronnie T. Trinh, Clarissa Aguilar, Hoang Vu Pham, Bojana Milutinovic, James M. Nichols, Rajasekaran Mahalingam, Andrew J. Shepherd
Summary: This study demonstrates that colon cancer is associated with latent sensory neuron dysfunction and implicates cytokine/chemokine signaling in this process. These findings may have implications for determining risk factors and treatment responsiveness related to neuropathy in colorectal cancer.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Multidisciplinary Sciences
Aleksey M. Nagornykh, Marina A. Tyumentseva, Aleksandr I. Tyumentsev, Vasiliy G. Akimkin
Summary: This study aims to evaluate the current methods of isolating and culturing hepatocellular carcinoma cells and assess the efficacy of tumor engraftment in mice. It will be the first extensive comparative study in this field.
Article
Geriatrics & Gerontology
Wouter R. P. H. van de Worp, Jan Theys, Alba Sanz Gonzalez, Brent van der Heyden, Frank Verhaegen, Duncan Hauser, Florian Caiment, Hubertus J. M. Smeets, Annemie M. W. J. Schols, Ardy van Helvoort, Ramon C. J. Langen
Summary: A syngeneic, orthotopic lung cancer mouse model was evaluated to see if it replicates the systemic and muscle-specific alterations associated with human lung cancer cachexia.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2023)
Article
Multidisciplinary Sciences
Junli Chang, Fulai Zhao, Xingyuan Sun, Xiaoping Ma, Wenlan Zhi, Yanping Yang
Summary: Osteosarcoma, the most common primary bone cancer in children and adolescents, often metastasizes to the lungs with a low five-year survival rate of less than 30%. Mouse models mimicking osteosarcoma development in humans have been utilized to study the fundamental mechanisms of osteosarcoma and pulmonary metastasis, and to develop novel therapeutics. These mouse models, created through intratibial injection of osteosarcoma cells, allow for monitoring and quantifying the growth and metastasis of osteosarcoma, making them ideal for exploring specific factors in osteosarcoma carcinogenesis and pulmonary metastasis.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Immunology
Chih-Wen Wang, Hsin-Ying Clair Chiou, Szu-Chia Chen, Da-Wei Wu, Hung-Hsun Lin, Huang-Chi Chen, Wei-Ting Liao, Ming-Hong Lin, Chih-Hsing Hung, Chao-Hung Kuo
Summary: This study investigates the effects of arsenic exposure on lung fibrotic changes. The results show that prolonged exposure to arsenic causes lung fibrosis and inhibiting epithelial-mesenchymal transition (EMT) can prevent arsenic-induced pulmonary fibrosis and damage.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Respiratory System
Thomas Glaab, Armin Braun
Summary: Using noninvasive head-out body plethysmography for measuring lung function in mice allows accurate assessment of airway responsiveness without anesthesia and with easy repeatability. EF50 as a marker of airflow limitation shows good agreement with invasive measurements, suitable for simple screening in large-scale studies and investigating the impact of respiratory infections on lung physiology in laboratory mice.
Article
Medicine, Research & Experimental
Dongliang Zhang, Ying Wang, Lei Liu, Zeng Li, Shengke Yang, Weixin Zhao, Xiang Wang, Hong Liao, Shukui Zhou
Summary: This study established prostate cancer models using cell sheet technology and compared their efficiency of tumor formation with cell suspension injection. The results showed that the orthotopic tumor formation rate in the cell sheet injection group was significantly better than that in the cell suspension injection group. Furthermore, the tumors in the cell sheet injection group had a higher density of micro-vessels, more collagen deposition, and a lower apoptotic rate. Cell sheet technology has potential applications in preclinical drug development, drug resistance mechanisms, and individualized therapy for prostate cancer.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, Research & Experimental
Lei Han, Yuning Huang, Qiang Fu, Yishu Xue, Fengming Ding, Min Zhang
Summary: Pseudomonas aeruginosa infection contributes to the progression of COPD, as an early Th1-related inflammation gradually shifts to a later Th2-related inflammation, and small-airway function decline occurs earlier than that of large-airway function.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Pharmacology & Pharmacy
Birte Ohm, Isabelle Moneke, Wolfgang Jungraithmayr
Summary: CD26/DPP4 plays a significant role in fibroproliferative disorders, and inhibiting CD26/DPP4 can reduce fibrotic changes. CD26/DPP4 inhibitors have the potential to be an effective treatment for fibrosis and have been proven to be safe in clinical use.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Jan Van Slambrouck, Mona Khan, Erik Verbeken, Sumin Choi, Vincent Geudens, Cedric Vanluyten, Simon Feys, Emiel Vanhulle, Elke Wollants, Kurt Vermeire, Charlotte De Fays, Lucia Aversa, Janne Kaes, Dirk Van Raemdonck, Robin Vos, Bart Vanaudenaerde, Gert De Hertogh, Els Wauters, Joost Wauters, Laurens J. Ceulemans, Peter Mombaerts
Summary: A study introduces a methodology for visualizing the replication of SARS-CoV-2 in the lungs at single-cell resolution. The method utilizes fluorescence staining and confocal imaging to observe viral replication in histological sections. This technique can be valuable for studying SARS-CoV-2 variants and other respiratory viruses.
Article
Psychiatry
Margaux Evenepoel, Matthijs Moerkerke, Nicky Daniels, Viktoria Chubar, Stephan Claes, Jonathan Turner, Bart Vanaudenaerde, Lynn Willems, Johan Verhaeghe, Jellina Prinsen, Jean Steyaert, Bart Boets, Kaat Alaerts
Summary: This study found that alterations in the brain's oxytocinergic system play an important role in the pathophysiology of autism spectrum disorder (ASD). Children with ASD displayed diminished oxytocin levels in the morning and a rise in oxytocin levels in the afternoon, which was associated with higher stress-induced cortisol release. Additionally, the study found differences in DNA methylation related to cortisol regulation in children with ASD.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Respiratory System
Vincent Geudens, Jan Van Slambrouck, Gitte Aerts, Lynn Willems, Tinne Goos, Janne Kaes, Andrea Zajacova, Iwein Gyselinck, Celine Aelbrecht, Astrid Vermaut, Hanne Beeckmans, Marie Vermant, Charlotte De Fays, Annelore Sacreas, Lucia Aversa, Michaela Orlitova, Arno Vanstapel, Ivan Josipovic, Matthieu N. Boone, John E. McDonough, Birgit Weynand, Charles Pilette, Wim Janssens, Lieven Dupont, Wim A. Wuyts, Geert M. Verleden, Dirk E. van Raemdonck, Robin Vos, Ghislaine Gayan-Ramirez, Laurens J. Ceulemans, Bart M. Vanaudenaerde
Summary: This study assessed disease progression in COVID-19 patients using in vivo CT and ex vivo microCT, and described the changes in lung volume and density as well as airway morphology. Results showed that COVID-19 lung volumes decreased and densities increased over time, mainly in the lower lobes. Ex vivo microCT revealed a 60% reduction in lung volume compared to controls, and increased visibility of airways in COVID-19 lungs, possibly due to fibrosis and increased airway diameter. Small-partition microCT further showed deformations in airway morphology and heterogeneous fibrotic organization in severely affected regions, which was confirmed by histology.
JOURNAL OF THORACIC DISEASE
(2023)
Article
Respiratory System
Xin Jin, Cedric Vanluyten, Michaela Orlitova, Jan Van Slambrouck, Robin Vos, Geert M. Verleden, Laurent Godinas, Arne P. Neyrinck, Catherine Ingels, Bart M. Vanaudenaerde, Paul De Leyn, Hans Van Veer, Lieven Depypere, Yi Zhang, Dirk E. M. Van Raemdonck, Laurens J. Ceulemans
Summary: This study demonstrated the feasibility and safety of off-pump re-LTx avoiding clamshell incision in selected patients. Compared to the more invasive approaches, clamshell-avoiding off-pump re-LTx resulted in shorter operative time and lower incidence of grade 3 primary graft dysfunction.
JOURNAL OF THORACIC DISEASE
(2023)
Article
Immunology
Charlotte de Fays, Vincent Geudens, Iwein Gyselinck, Pieterjan Kerckhof, Astrid Vermaut, Tinne Goos, Marie Vermant, Hanne Beeckmans, Janne Kaes, Jan Van Slambrouck, Yousry Mohamady, Lynn Willems, Lucia Aversa, Emanuela E. Cortesi, Charlotte Hooft, Gitte Aerts, Celine Aelbrecht, Stephanie Everaerts, John E. McDonough, Laurens J. De Sadeleer, Sophie Gohy, Jerome Ambroise, Wim Janssens, Laurens J. Ceulemans, Dirk Van Raemdonck, Robin Vos, Tillie L. Hackett, James C. Hogg, Naftali Kaminski, Ghislaine Gayan-Ramirez, Charles Pilette, Bart M. Vanaudenaerde
Summary: This study investigated the mucosal immune defense in control and end-stage COPD lungs, and found dysregulation in mucosal defense, with decreased defensins and increased mucus and IgA responses. The study also observed CD8+ T-cell accumulation in mild emphysema zones and adaptive Th and B cell activation in areas with more advanced tissue destruction. The study suggests that innate immune alterations in COPD occur early in the tissue destruction process, affecting both the alveoli and the terminal bronchioles, before the onset of an adaptive immune response.
FRONTIERS IN IMMUNOLOGY
(2023)
Meeting Abstract
Critical Care Medicine
K. Wu, A. Moran-Macdonald, S. Ledoux, A. Hsieh, J. D. Cooper, W. L. Janssens, S. Everaerts, B. Vanaudenaerde, J. C. Hogg, D. M. Vasilescu, T. Hackett
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Meeting Abstract
Critical Care Medicine
C. De Fays, H. Beeckmans, P. Kerckhof, V. Geudens, A. Vermaut, I. Gyselinck, T. Goos, M. Vermant, J. Kaes, J. Van Slambrouck, Y. Mohamady, L. Willems, L. Aversa, K. Maes, C. Aelbrecht, S. Everaerts, J. E. Mcdonough, L. J. De Sadeleer, S. Gohy, J. Ambroise, W. A. Wuyts, W. L. Janssens, L. J. Ceulemans, D. E. M. Van Raemdonck, R. Vos, T. Hackett, J. C. Hogg, N. Kaminski, C. Pilette, G. Gayan-Ramirez, B. Vanaudenaerde
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Meeting Abstract
Critical Care Medicine
S. Gogaert, T. Goos, M. Vermant, M. S. Carlon, N. De Crem, L. De Sadeleer, E. De langhe, A. Dubbeldam, B. Vanaudenaerde, J. Verschakelen, B. Weynand, J. Yserbyt, A. Bertrand, M. De Vos, W. A. Wuyts
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Meeting Abstract
Critical Care Medicine
T. S. Adams, J. C. Schupp, A. Balayev, A. Justet, P. Sharma, G. DeIuliis, X. Yan, W. A. Wuyts, B. Vanaudenaerde, N. Kaminski
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
I. Moneke, J. Burkle, E. Pfaffendorf, P. Bronsert, G. Zissel, S. Faendrich, B. Passlick, S. Diederichs, W. Jungraithmayr
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
M. Orlitova, D. Van Beersel, A. E. Frick, K. Van de Voorde, K. Degezelle, J. Hellinck, M. Nolmans, B. M. Vanaudenaerde, G. M. Verleden, R. Vos, P. Claus, D. E. Van Raandonck, L. J. Ceulemans, T. Verbelen, A. P. Neyrinck
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
H. Beeckmans, P. Kerckhof, J. McDonough, L. De Sadeleer, J. Kaes, A. Sacreas, C. Aelbrecht, A. Vanstapel, K. Maes, H. Schoemans, E. Wauters, A. Neyrinck, G. Verleden, L. Dupont, L. Godinas, D. Van Raemdonck, B. Vanaudenaerde, R. Vos
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
C. Hooft, J. Kaes, T. Heigl, H. Beeckmans, P. Kerckhof, A. Vanstapel, X. Jin, J. Slambrouck, C. Vandervelde, D. Van Raemdonck, N. Kaminski, J. McDonough, L. Ceulemans, R. Vos, B. Vanaudenaerde
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2023)
Article
Biochemical Research Methods
Jan Van Slambrouck, Charlotte Schoenaers, Lies Laenen, Xin Jin, Kurt Beuselinck, Ann Verdonck, Joost Wauters, Geert Molenberghs, Bart M. Vanaudenaerde, Robin Vos, Peter Mombaerts, Katrien Lagrou, Laurens J. Ceulemans
Summary: The ability to detect SARS-CoV-2 RNA or antigen in BAL fluid samples using point-of-care tests (POCTs) can reduce the risk of developing severe COVID-19 after lung transplantation.
JOURNAL OF VIROLOGICAL METHODS
(2024)