Article
Pharmacology & Pharmacy
Ana Sofia Agonia, Ana Palmeira-de-Oliveira, Catarina Cardoso, Catia Augusto, Christian Pellevoisin, Christelle Videau, Ricardo Jorge Dinis-Oliveira, Rita Palmeira-de-Oliveira
Summary: The use of in vitro human skin permeation tests is valuable for evaluating the quality and equivalence of topical drug products. In this study, the use of reconstructed human epidermis (RhE) as a biological membrane for bioequivalence testing of clotrimazole creams demonstrated its discriminatory power and higher permeability compared to human skin.
Article
Toxicology
Christian Pellevoisin, Kelly P. Coleman, Sebastian Hoffmann
Summary: Irritation testing is a crucial part of biocompatibility assessment for medical devices, historically conducted on animals, but now reconstituted human epidermis-based assays are preferred for evaluating mild irritants in medical device extracts, as demonstrated in a 2018 study.
TOXICOLOGY IN VITRO
(2022)
Article
Environmental Sciences
Jee-hyun Hwang, Seungmi Lee, Ho Geon Lee, Dalwoong Choi, Kyung-Min Lim
Summary: The effects of acidic pH on skin need to consider both pH and concentration. Organic and inorganic acids can be irritants to the skin at certain concentrations. Alkaline agents also cause damage to the skin.
Article
Toxicology
Chih-Ching Liao, Cheng-Yi Wu, Meng-Hsueh Lin, Feng-Koo Hsieh, Lih-Tao Hsu, Shiun-Yin Chang, Kuan-Jen Chen, Hui-Ting Huang, Hui-Chun Hsu, Chiu-Hsing Lin, Pei-Ju Lin, Huey-Min Lai, Hajime Kojima, Hiroaki Todo, Sung-Jan Lin, Jih-Heng Li, Wannhsin Chen
Summary: A new reconstructed human epidermis model EPiTRI was developed for skin irritation test, showing good predictive capacity with 100% sensitivity, 70% specificity and 85% accuracy in testing 20 reference chemicals. An international validation study confirmed the reproducibility and predictive capacity of EPiTRI-SIT, with overall sensitivity, specificity and accuracy of 96%, 70% and 83% respectively.
TOXICOLOGY IN VITRO
(2021)
Article
Chemistry, Analytical
Manuel Chacon, Natalia Vazquez, Sergio Alonso-Alonso, Mairobi Persinal-Medina, Sara Llames, Marta Pevida, Ignacio Alcalde, Jesus Merayo-Lloves, Alvaro Meana
Summary: The rabbit skin irritation test has been the standard for evaluating chemical irritation potential, but alternative methods are encouraged. Reconstructed human epidermis (RhE) models mimic human epidermis and can be used as an alternative. As the use of RhE models increases, new challenges have emerged, such as the need for non-invasive and marker-free methodologies. Electrochemical impedance spectroscopy (EIS) is a promising method to meet these requirements.
Article
Environmental Sciences
Yang-Jee Kim, Dong-Hyuk Seo, Il-Soo Kim, Mi-Sook Jung, Jin-Young Bae, Moon-Yong Song, Kyung-Seuk Song, Jin-Sik Kim
Summary: The demand for alternatives to animal testing in evaluating eye irritation from ophthalmic medical devices has increased. This study evaluated the applicability of a human corneal model as an alternative method to test the safety of these devices. The proposed method using the MCTT HCETM model exhibited excellent reproducibility and predictive capacity, making it suitable for evaluating eye irritation caused by ophthalmic medical devices.
Article
Toxicology
C. Pellevoisin, F. Cottrez, J. Johansson, E. Pedersen, K. Coleman, H. Groux
Summary: The study found that the SENS-IS assay can accurately identify sensitizers in medical device extracts, potentially replacing current in vivo assays.
TOXICOLOGY IN VITRO
(2021)
Article
Medicine, Research & Experimental
Patricia Zoio, Sara Lopes-Ventura, Joana Marto, Abel Oliva
Summary: The open-source full-thickness skin model (FTSm) developed in this study exhibited comparable structural and barrier properties to native human skin, and outperformed commercial models. These models are of great importance in accelerating the development and dissemination of alternatives to animal testing.
ALTEX-ALTERNATIVES TO ANIMAL EXPERIMENTATION
(2022)
Article
Environmental Sciences
Joyce V. B. Borba, Vinicius M. Alves, Rodolpho C. Braga, Daniel R. Korn, Kirsten Overdahl, Arthur C. Silva, Steven U. S. Hall, Erik Overdahl, Nicole Kleinstreuer, Judy Strickland, David Allen, Carolina Horta Andrade, Eugene N. Muratov, Alexander Tropsha
Summary: Modern chemical toxicology is in need of reducing, refining, and replacing animal tests. In this study, a collection of computational models called STopTox was developed to predict the toxicity hazard of small organic molecules. The models were validated and shown to have high accuracy. A web portal was also established to assist scientists and regulators in identifying potential toxicants or non-toxicants in chemical libraries of interest.
ENVIRONMENTAL HEALTH PERSPECTIVES
(2022)
Article
Dermatology
Xiao Ma, Feifei Wang, Bo Wang
Summary: The use of 3D skin models for skin irritation testing of cosmetic products provides a more accurate evaluation of the safety and efficacy of the products. The study found that certain cosmetic products can cause skin irritation based on tissue activity and IL-1 alpha expression, highlighting the importance of using 3D skin models for such evaluations.
JOURNAL OF COSMETIC DERMATOLOGY
(2021)
Article
Geriatrics & Gerontology
Julia T. Oliveira, Vanja Dakic, Gabriela Vitoria, Carolina da S. G. Pedrosa, Mayara Mendes, Luiz Guilherme H. S. Aragao, Thyago R. Cardim-Pires, Damien Lelievre, Daniel Rodrigues Furtado, Roberta O. Pinheiro, Debora Foguel, Lionel Breton, Charbel Bouez, Rodrigo De Vecchi, Marilia Zaluar P. Guimaraes, Stevens Rehen
Summary: This study reveals that oligomeric alpha-Synuclein (O alpha-Syn) induces inflammation and degeneration in the skin, leading to a decrease in keratinocyte proliferation and thickness of the epidermis. These findings shed light on the potential role of O alpha-Syn in the aging process of the skin.
NEUROBIOLOGY OF AGING
(2022)
Article
Toxicology
Roland Wedekind, Anja Fischer, Karsten R. Mewes, Peter Brenneisen, Olaf Holtkotter
Summary: Under the current EU chemicals legislation, in vitro skin models are widely used to determine the skin irritation potential of chemicals and mixtures. For surfactants, it is known that the irritation potential of a mixture is usually lower than that of the individual surfactants, which is called surfactant antagonism. In this study, the irritation potential of surfactants and their mixtures were evaluated using the Open Source Reconstructed Epidermis models, and it was found that the mixtures showed reduced irritation potential due to their lower ability to damage the skin model's barrier.
TOXICOLOGY IN VITRO
(2023)
Article
Toxicology
Takami Akagi, Tomomi Yamada, Hiromi Miyazaki, Hiroyuki Taguchi, Hidefumi Ikeda, Masakazu Katoh, Simona Mura, Patrick Couvreur, Paninee Chetprayoon, Rawiwan Maniratanachote, Hiroaki Yoshida, Hiroharu Ajiro, Koji Hashimoto, Takao Ashikaga, Hajime Kojima, Mitsuru Akashi
Summary: The aim of this study is to validate a skin irritation test using three-dimensional reconstructed human epidermal skin equivalents prepared by a layer-by-layer method. The validation study showed that the developed skin model has similar characteristics to validated reference methods and native human skin. The results obtained from the study demonstrated the reproducibility and accuracy of the test in distinguishing skin irritants from nonirritants.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Elisabeth Hofmann, Anna Schwarz, Julia Fink, Lars-Peter Kamolz, Petra Kotzbeck
Summary: The skin acts as a protective barrier and controls the transport of water, ions, and nutrients. Animal experiments are commonly used to study skin structure and function, but anatomical and physiological differences between species can limit the translatability of data to humans. In vitro models, such as reconstructed human skin, provide valuable alternatives to animal experiments. This review highlights the progress made in establishing complex in vitro skin models.
Article
Dermatology
Romana Sinova, Vojtech Pavlik, Matej Simek, Martin Cepa, Martin Ondrej, Kristina Nesporova, Vladimir Velebny
Summary: Exposure to the sun can lead to UV-induced skin damage, and this study investigated the changes in hyaluronan (HA) metabolism in the skin after UV exposure. The results showed that UV irradiation increased the amount of HA in the skin and altered the expression of related genes and proteins. These findings suggest that UV exposure affects HA metabolism in the skin and that this response may be modulated by the underlying dermis.
EXPERIMENTAL DERMATOLOGY
(2023)
Article
Toxicology
Kathryn Page, Walter Westerink, Kristie Sullivan, Thomas McDonald, Clive Roper
Summary: This study developed a new method to assess the skin irritation of antimicrobial cleaning products. The method utilized a more human-like model and demonstrated its effectiveness through comparison with in vivo rabbit skin irritation data.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Arupam Patra, Thirukumaran Kandasamy, Siddhartha Sankar Ghosh, Gurvinder Kaur Saini
Summary: This study successfully produced recombinant anisoplin and demonstrated its significant anti-cancer effect and ability to induce apoptosis in breast cancer cells. The activation of related signaling pathways may be the key to cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Thomas Haller, Alexander Jesacher, Alberto Hidalgo, Christina Schmidt
Summary: This study used live cell imaging to observe the accumulation of amiodarone in primary rat alveolar type II cells, and found that it specifically accumulates in lamellar bodies. The uptake is rapid, while storage is persistent. The main mechanisms for intracellular bioaccumulation of amiodarone are proposed to be passive diffusion, ion-trapping, and lipophilic interactions.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Filiz Bakar-Ates, Erva Ozkan
Summary: This study investigated the ferroptotic effect of CuB in breast cancer cells and evaluated its combination with erastin, a ferroptosis inducer. The results showed that the combination treatment significantly activated the ferroptotic pathways and altered the expression of iron-related proteins in breast cancer cells.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Rachel Z. Bennie, Ian C. Shaw
Summary: Estrogen mimics are synthetic and naturally occurring compounds that can interact with estrogen receptors in animals. In vitro transactivation reporter gene assay and in silico molecular modelling can be used to predict the mimicry of these compounds, reducing reliance on animal studies.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Sevda Isik, Semra Cicek
Summary: This study found that high doses of riboflavin can cause cytotoxicity in rainbow trout gonad cells and affect the transcriptional expressions of antioxidant enzymes and growth and reproductive genes, potentially leading to DNA damage and cell death.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Ying Wang, Jie Wu, Mengqi Wan, Dou Yang, Fasheng Liu, Kehao Li, Manxin Hu, Yuanyuan Tang, Huiqiang Lu, Shouhua Zhang, Yuanzhen Xiong
Summary: m-Cresol is commonly used as an intermediate for pesticides and other industrial applications. This study investigated the hepatotoxicity of m-cresol using zebrafish larvae and explored its molecular mechanisms. The results suggest that m-cresol may induce liver damage in zebrafish larvae through oxidative stress and cell apoptosis pathways.
TOXICOLOGY IN VITRO
(2024)
Article
Toxicology
Vincent J. Manna, Shannon Dwyer, Vanessa Pizutelli, Salvatore J. Caradonna
Summary: The widespread use of electronic cigarettes and the emergence of a new illness have raised concerns about the effects of e-cigarette vapor on respiratory tissues. Researchers have developed a simple device to mimic the response of human airway tissue after long-term exposure to e-cigarette vapor, and have identified differences in the effects of different vapor compositions on airway tissue.
TOXICOLOGY IN VITRO
(2024)