Article
Engineering, Biomedical
Weijing Han, Rania El Botty, Elodie Montaudon, Laurent Malaquin, Frederic Deschaseaux, Nicolas Espagnolle, Elisabetta Marangoni, Paul Cottu, Gerard Zalcman, Maria Carla Parrini, Franck Assayag, Luc Sensebe, Pascal Silberzan, Anne Vincent-Salomon, Guillaume Dutertre, Sergio Roman-Roman, Stephanie Descroix, Jacques Camonis
Summary: Researchers have successfully developed an in vitro breast cancer bone-metastasis model using 3D printing technology to print a bone scaffold and successfully implanting patient-derived breast cancer bone metastatic cells. This experimental system can mimic the characteristics of the bone microenvironment and cancer bone metastasis, which has implications for drug testing.
Article
Engineering, Biomedical
Mansoor Chaaban, Adrien Moya, Andres Garcia-Garcia, Robert Paillaud, Romain Schaller, Thibaut Klein, Laura Power, Katarzyna Buczak, Alexander Schmidt, Elisabeth Kappos, Tarek Ismail, Dirk J. Schaefer, Ivan Martin, Arnaud Scherberich
Summary: This study investigates the effects of cell expansion and chondrogenic maturation degree on the process of endochondral ossification (ECO). The results show that the maturation degree of adipose-derived stromal cells (ASC) is closely related to their ability to form bone in vivo, with ECO achieved only for HCTs of intermediate maturation. Meanwhile, the expansion of ASC also results in the enrichment of the stromal fraction, and inducing quiescence can rescue their chondrogenic potential.
Article
Engineering, Biomedical
Bregje W. M. de Wildt, Lizzy A. B. Cuypers, Esther E. A. Cramer, Annelieke S. Wentzel, Keita Ito, Sandra Hofmann
Summary: In vitro bone remodeling models using osteoclast-osteoblast cocultures can be used to study human bone remodeling and reduce the need for animal experiments. However, the optimal culture conditions for supporting both cell types are still unknown. This study evaluated the impact of culture variables on bone turnover outcomes using a human bone remodeling model. The results provide insights into the translation between in vitro and in vivo studies for better drug development.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Multidisciplinary Sciences
Wojciech J. Szlachcic, Agnieszka Dabrowska, Aleksandra Milewska, Natalia Ziojla, Katarzyna Blaszczyk, Emilia Barreto-Duran, Marek Sanak, Marcin Surmiak, Katarzyna Owczarek, Dariusz Grzanka, Julia Durzynska, Krzysztof Pyrc, Malgorzata Borowiak
Summary: Recent studies have found that SARS-CoV-2 can infect the adult human pancreas and cause damage. In this study, it was discovered that the receptors ACE2, TMPRSS2, and NRP1, which are necessary for SARS-CoV-2 infection, are expressed in the precursor cells of insulin-producing pancreatic beta-cells. The study also revealed that SARS-CoV-2 can efficiently enter and replicate in human multipotent pancreatic and endocrine progenitors. Interestingly, the mechanism of viral entry in pancreatic cells involves cathepsin-dependent endocytosis, which differs from the entry route in the respiratory tract. These findings have implications for studying potential drugs to inhibit viral entry and understanding the impact of SARS-CoV-2 infection on pancreas development.
Article
Endocrinology & Metabolism
Francesca Salamanna, Veronica Borsari, Stefania Pagani, Giovanni Barbanti Brodano, Alessandro Gasbarrini, Milena Fini
Summary: A novel and advanced model of breast cancer bone metastasis was developed using fresh tissue from patients, maintaining tissue viability and molecular markers long-term. This model has the potential to be used as an alternative for cancer research and drug testing, predicting patient-specific responses.
Article
Multidisciplinary Sciences
Maaike V. J. Braham, Rob S. van Binnendijk, Anne-Marie M. Buisman, Reina E. Mebius, Jelle de Wit, Cecile A. C. M. van Els
Summary: This study aimed to improve in vitro germinal center (GC) simulation by using 3D culture techniques. Human B-cells were incorporated into PEG-4MAL hydrogels, supported by CD40L cells, human tonsil-derived stromal cells, and cytokines. The 3D culture significantly increased plasmablast and plasma cell numbers, promoted antibody production, and enabled class switching of naive B-cells. Formation of large B-cell clusters resembling GC-like structures was also observed. In conclusion, this well-characterized and controllable hydrogel-based 3D lymphoid model supports enhanced B-cell survival, proliferation, differentiation, and antibody production.
Review
Pharmacology & Pharmacy
I Yuste, F. C. Luciano, E. Gonzalez-Burgos, A. Lalatsa, D. R. Serrano
Summary: Understanding the biology and behavior of human osteoblasts in vitro is crucial for developing research models that replicate bone structure and interactions accurately. Mimicking the bone microenvironment is challenging yet essential for clinical translation of novel medicines. Advanced technologies like 3D bioprinting and microfluidics bridge the gap between in vitro and in vivo models.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Immunology
Rene Reitermaier, Thomas Krausgruber, Nikolaus Fortelny, Tanya Ayub, Pablo Augusto Vieyra-Garcia, Philip Kienzl, Peter Wolf, Anke Scharrer, Christian Fiala, Marita Kolz, Manuela Hiess, Martin Vierhapper, Christopher Schuster, Andreas Spittler, Christof Worda, Wolfgang Weninger, Christoph Bock, Rene Reitermaier, Adelheid Elbe-Buerger
Summary: Single-cell analyses identified a naive T cell population expressing specific T cell receptors enriched in fetal skin and intestine. These cells may contribute to early skin development and fetal immune defense, showing fundamental differences in immune surveillance between fetal and adult human skin.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biology
Aref Saberi, Albert P. Aldenkamp, Nicholas A. Kurniawan, Carlijn V. C. Bouten
Summary: This research developed a method to engineer cerebral tissues with intact neuronal networks, mimicking neuropathological signatures such as epileptiform discharges. By promoting cell reaggregation and using a multi-chambered tissue culture chip, the study successfully simulated brain network disorders.
COMMUNICATIONS BIOLOGY
(2022)
Review
Immunology
Nicoletta Manduca, Ester Maccafeo, Ruggero De Maria, Antonella Sistigu, Martina Musella
Summary: Cancer immunotherapy, despite showing extended progression-free survival in cancer treatment compared to conventional therapies, is effective in only a minority of patients. To improve its applicability, it is crucial to overcome obstacles such as the lack of preclinical models that accurately depict the local tumor microenvironment (TME), which significantly influences disease onset, progression, and response to therapy. This review provides a comprehensive overview of current 3D models, including tumor spheroids, organoids, and immune Tumor-on-a-Chip models, that mimic the complexity and dynamics of the TME. It emphasizes the importance of understanding the TME as a major target in anticancer therapy and highlights the advantages, translational potentials, challenges, and limitations of these models.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Lena-Christin Ingwersen, Marcus Frank, Hendrik Naujokat, Klaas Loger, Rainer Bader, Anika Jonitz-Heincke
Summary: Long-term exposure to BMP-2 promotes osteogenic differentiation and adipogenesis, and regulates mediators involved in osteoclast activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Erik van der Wal, Alessandro Iuliano, Stijn L. M. in't Groen, Anjali P. Bholasing, Dominik Priesmann, Preeti Sharma, Bianca den Hamer, Vittorio Saggiomo, Marcus Kruger, W. W. M. Pim Pijnappel, Jessica C. de Greef
Summary: Researchers have successfully differentiated purified myogenic progenitor cells with high contractile properties from feeder-free hiPSCs, resulting in tissue engineered skeletal muscles with forces similar to primary myoblast-derived tissues. Gene expression and proteomic analysis showed a similar profile of proteins involved in myogenic differentiation and sarcomere function between hiPSC-derived and primary myoblast-derived 3D tissues. This protocol could be used for studying personalized human skeletal muscle tissue in health and disease.
Article
Engineering, Biomedical
Byoung Soo Kim, Minjun Ahn, Won-Woo Cho, Ge Gao, Jinah Jang, Dong-Woo Cho
Summary: The study successfully developed a 3D diseased skin tissue model with pathophysiological hallmarks of type 2 diabetes using 3D cell printing technique. By incorporating a perfusable vascularized diabetic hypodermis, the structural similarities and diabetic properties of the model were enhanced. The feasibility of using this new disease model for drug development was demonstrated through the application of test drugs.
Review
Engineering, Biomedical
Minjun Ahn, Won-Woo Cho, Wonbin Park, Jae-Seong Lee, Min-Ju Choi, Qiqi Gao, Ge Gao, Dong-Woo Cho, Byoung Soo Kim
Summary: Human skin, as the outermost organ of the body, shows visible signs of physiological health. The need for alternative in vitro platforms that mimic the structural and functional characteristics of natural skin has increased due to ethical concerns and genetic differences in conventional animal studies. Different reproducible biofabrication strategies are required to reproduce the pathological features of diseased human skin compared to those used for healthy-skin models.
BIOMATERIALS RESEARCH
(2023)
Article
Cell Biology
Carlo Brighi, Federico Salaris, Alessandro Soloperto, Federica Cordella, Silvia Ghirga, Valeria de Turris, Maria Rosito, Pier Francesca Porceddu, Chiara D'Antoni, Angelo Reggiani, Alessandro Rosa, Silvia Di Angelantonio
Summary: The study reveals that the requirement for the FMRP protein is to correctly support neuronal and glial cell proliferation, and to set the correct excitation/inhibition ratio in human brain development.
CELL DEATH & DISEASE
(2021)
Review
Chemistry, Multidisciplinary
Miriam Filippi, Francesca Garello, Oncay Yasa, Jesil Kasamkattil, Arnaud Scherberich, Robert K. Katzschmann
Summary: The article discusses the applications of magnetic nanoparticles in biomedicine and their combination with polymers to form magnetic nanocomposites. These composites have the potential to modulate various cellular functions and show promise in regenerative medicine and the development of biomedical robots.
Article
Biochemistry & Molecular Biology
Zihe Huo, Remo Bilang, Claudiu T. Supuran, Nicolas von Der Weid, Elisabeth Bruder, Stefan Holland-Cunz, Ivan Martin, Manuele G. Muraro, Stephanie J. Gros
Summary: This study proposes a new patient-derived 3D slice-culture perfusion-based model for neuroblastoma treatment evaluation, combined with isothermal microcalorimetry. The use of the novel carbonic anhydrase IX and XII (CAIX/CAXII) inhibitor SLC-0111 showed promising results in reducing cell viability and thermogenesis in neuroblastoma cells. The perfusion-based bioreactor system allowed for the evaluation of drug response with intact tumor cells and stromal structure for up to 7 days. This approach can facilitate the development of personalized treatment strategies for neuroblastoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Stefania Scialla, Fabiana Gullotta, Daniela Izzo, Barbara Palazzo, Francesca Scalera, Ivan Martin, Alessandro Sannino, Francesca Gervaso
Summary: Articular cartilage degeneration is a challenging issue with limited repair capabilities. Tissue engineering strategies using appropriate scaffolds for chondrogenic differentiation of stem cells can be a promising solution. This study investigated the influence of two genipin crosslinking routes on collagen-based scaffolds and found that bulk crosslinking with genipin resulted in a more uniform and homogeneous deposition of chondral-like matrix.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Cell & Tissue Engineering
Julien Guerrero, Boris Dasen, Agne Frismantiene, Sebastien Pigeot, Tarek Ismail, Dirk J. Schaefer, Maria Philippova, Therese J. Resink, Ivan Martin, Arnaud Scherberich
Summary: The presence of T-cadherin-positive cells derived from the stromal vascular fraction (SVF) is essential for the osteogenic and vasculogenic properties of SVF cells. The crosstalk between stromal cells and T-cadherin-expressing endothelial cells within adipose tissue critically regulates osteogenesis.
STEM CELLS TRANSLATIONAL MEDICINE
(2022)
Article
Biochemical Research Methods
Gordian Born, Evelia Plantier, Guido Nannini, Alessandro Caimi, Andrea Mazzoleni, M. Adelaide Asnaghi, Manuele G. Muraro, Arnaud Scherberich, Ivan Martin, Andres Garcia-Garcia
Summary: Bioreactors that allow direct perfusion of cell suspensions or culture media through 3D scaffolds are widely used in tissue engineering to improve cell seeding efficiency, uniformity of cell distribution, and tissue development. A macro-scale U-shaped bioreactor has been previously developed, but the perfusion flow in the peripheral regions was not uniform. In this study, the design of the perfusion chamber has been optimized to provide more homogeneous perfusion flow through the scaffold. Furthermore, a miniaturized version of the flow-optimized bioreactor has been proposed for medium-throughput assays.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Clinical Neurology
Brian A. Karamian, Gregory D. Schroeder, Mark J. Lambrechts, Jose A. Canseco, Cumhur Oner, Emiliano Vialle, Shanmuganathan Rajasekaran, Marcel R. Dvorak, Lorin M. Benneker, Frank Kandziora, Klaus Schnake, Christopher K. Kepler, Alexander R. Vaccaro
Summary: The purpose of this study was to validate the AO Spine Subaxial Injury Classification System, and the results showed that the system had excellent interobserver reliability and intraobserver reproducibility for fracture morphology, substantial reliability and reproducibility for facet injuries, and excellent reproducibility with substantial reliability for injury subtype. In conclusion, this classification system is accurate and reliable in assessing and describing subaxial cervical spine injuries.
EUROPEAN SPINE JOURNAL
(2023)
Article
Engineering, Biomedical
Yawei Gu, Sebastien Pigeot, Lucas Ahrens, Fabian Tribukait-Riemenschneider, Melika Sarem, Francine Wolf, Andres Garcia-Garcia, Andrea Barbero, Ivan Martin, V. Prasad Shastri
Summary: By developing a bioink system with appropriate rheological properties and using 3D bioprinting technology, it is possible to create high cell density structures of predefined geometry, which can then be implanted into nude mice to generate bone tissue with neovascularization. This method demonstrates the potential for fabricating complex bone tissue for clinical applications.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell Biology
Boris Dasen, Sebastien Pigeot, Gordian Manfred Born, Sophie Verrier, Olga Rivero, Petra S. Dittrich, Ivan Martin, Maria Filippova
Summary: This study found that pericytes express not only the classical N-cadherin, but also the atypical T-cadherin. T-cadherin plays an important role in regulating the proliferation, migration, invasion, and interactions of pericytes with endothelial cells.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell & Tissue Engineering
Sowmya Viswanathan, Katarina Le Blanc, Rachele Ciccocioppo, Georges Dagher, Anthony J. Filiano, Jacques Galipeau, Mauro Krampera, Lena Krieger, Manoj M. Lalu, Jan Nolta, Viviana Marcela Rodriguez Pardo, Yufang Shi, Karin Tarte, Daniel J. Weiss, Ivan Martin
Summary: The International Standards Organization (ISO) has recently published ISO standardization documents for MSC(WJ) and MSC(M) biobanking, which standardize the terminology and functional characterization of MSCs. These documents, developed with input from ISCT, provide requirements and recommendations for functional characterization of MSC(WJ) and MSC(M) and represent an international consensus on MSC identity and characterization. They are an important first step in standardizing MSC biobanking and characterization for research and development.
Article
Cell & Tissue Engineering
Anke Wixmerten, Sylvie Miot, Patrick Bittorf, Francine Wolf, Sandra Feliciano, Stephan Hackenberg, Sebastian Hasner, Werner Krenger, Martin Haug, Ivan Martin, Oliver Pullig, Andrea Barbero
Summary: This article describes the regulatory compliant change of a raw material in the development of a medicinal product. The substitution of autologous serum with clinical-grade human platelet lysate (hPL) in the manufacturing process was shown to enhance cell proliferation and gene expression without significant changes in product quality. The modified process was accepted by national competent authorities and applied in ongoing clinical tests of N-TEC.
Article
Biotechnology & Applied Microbiology
Jesil Kasamkattil, Anna Gryadunova, Raphael Schmid, Max Hans Peter Gay-Dujak, Boris Dasen, Morgane Hilpert, Karoliina Pelttari, Ivan Martin, Stefan Scharen, Andrea Barbero, Olga Krupkova, Arne Mehrkens
Summary: An advanced 3D microtissues model containing human degenerating nucleus pulposus tissue was developed to mimic the microenvironment of early-stage degenerative disc disease. Nasal chondrocytes in spheroidal organization showed superior regenerative performance compared to cell suspension, and pre-conditioning with IL-1Ra further improved their ability to counteract inflammation and support new matrix production. This study is of great importance for therapeutic strategies in degenerative disc disease.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Andres Garcia-Garcia, Sebastien Pigeot, Ivan Martin
Summary: The contribution of the immune system to tissue regeneration is increasingly recognized, suggesting that tissue engineering strategies and biomaterials design should focus on regulating early immunological processes. This study aimed to enhance the osteoinductivity of engineered extracellular matrices (ECMs) by enriching their repertoire of immunomodulatory factors. The results showed that priming with IL1 beta and overexpressing BMP-2 enabled the engineering of ECMs with preserved osteoinductive signals and increased levels of angiogenic and pro-inflammatory molecules. These IL1 beta-induced ECMs enhanced the inflammatory phase and ultimately led to faster and more efficient bone formation.
BIOACTIVE MATERIALS
(2023)
Article
Orthopedics
M. Kamenova, E. Li, J. Soleman, O. Fiebig, A. Mehrkens, S. Schaeren
Summary: The aim of this study was to compare reoperation rates and clinical outcomes between TLIF and posterior stabilization with PEEK rods in elderly patients with one-level lumbar spine degenerative disease. The results showed similar reoperation rates and outcomes between the TLIF and PEEK groups, but the TLIF group had higher intraoperative blood loss, administration of tranexamic acid, and operation time. In patients above 80 years of age, the TLIF group had higher reoperation rates as well.
ARCHIVES OF ORTHOPAEDIC AND TRAUMA SURGERY
(2023)
Article
Surgery
Joel Buergin, Lucas Werth, Rene Largo, Arnaud Scherberich, Dirk J. Schaefer, Alexandre Kaempfen
Summary: The study explored the vascularization pattern of human adipofascial flaps and identified a high density of blood vessels on the superficial and near fascia, as well as a secondary plexus. It is recommended to maintain at least 2-3 mm of subcutaneous fatty tissue on the fascia to benefit from both plexuses.
PLASTIC AND RECONSTRUCTIVE SURGERY-GLOBAL OPEN
(2022)
