Journal
TISSUE ENGINEERING PART C-METHODS
Volume 24, Issue 6, Pages 313-321Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.tec.2018.0031
Keywords
mesenchymal stem cells; marrow stromal cells; senescence; pediatric mesenchymal stem cells; differentiation; MSC immunophenotype; immunomodulation
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Funding
- AO Foundation, Switzerland [AOCMF-15-27F]
- VENI grant by STW [13659]
- Erasmus MC grant
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Mesenchymal stem cells/marrow stromal cells (MSCs) are attractive for applications ranging from research and development to use in clinical therapeutics. However, the most commonly studied MSCs, adult bone marrow MSCs (A-MSCs), are limited by significant donor variation resulting in inconsistent expansion rates and multilineage differentiation capabilities. We have recently obtained permission to isolate pediatric MSCs (P-MSCs) from surplus iliac crest bone chips. Here, we developed a simple and easily replicable isolation protocol yielding P-MSCs, which adhere to MSC defining guidelines. After confirming immunophenotypic marker expression, we compared expansion rates, senescence, morphology, and trilineage differentiation of P-MSCs to A-MSCs for multiple donors. We found P-MSCs have faster in vitro replication, consistently show significantly lower senescence, and are capable of more reproducible multilineage differentiation than A-MSCs. We, therefore, believe P-MSCs are a promising candidate for use in research applications and potentially as part of an allogeneic therapeutic treatment.
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