Article
Engineering, Biomedical
Bin Jiang, Weijie Li, Samantha Stewart, Wenquan Ou, Baolin Liu, Pierre Comizzoli, Xiaoming He
Summary: The study found that natural sand can seed ice above -10 degrees C, allowing hiPSCs to be cryopreserved without serum and with reduced cryoprotectants, leading to high cell survival. The cryopreserved hiPSCs also retained high pluripotency and functions.
BIOACTIVE MATERIALS
(2021)
Article
Chemistry, Medicinal
Dilaware Khan, Ann-Christin Nickel, Sebastian Jeising, Constanze Uhlmann, Sajjad Muhammad, Daniel Haenggi, Igor Fischer, Ulf Dietrich Kahlert
Summary: The study demonstrates that frozen cells do not alter their drug response phenotypes, supporting the use of human induced pluripotent stem cells as a reliable platform for in vitro pharmacology. Even in frozen cells with stable overexpression of oncogenes, there were no relative changes observed in their drug response.
Article
Cell & Tissue Engineering
Renata Gilfanova, Kirsten M. Auclair, Alvin Hui, Philip J. Norris, Marcus O. Muench
Summary: The study investigated the cryopreservation of human bone marrow using reduced concentrations of DMSO and found that 5% DMSO was as effective as higher concentrations in preserving hematopoietic stem cells. Different methods of analyzing stem cell engraftment in mice showed consistent results in supporting the cryopreservation of HSCs with reduced concentrations of DMSO.
Article
Computer Science, Interdisciplinary Applications
Yusuke Hayashi, Ikki Horiguchi, Masahiro Kino-oka, Hirokazu Sugiyama
Summary: This study presents a model-based assessment of temperature profiles in slow freezing for human induced pluripotent stem cells, which can estimate cell survival rate and required freezing time based on given freezing temperature profiles to achieve quality and productivity objectives. Simulation results indicate different cooling rates in dehydration, nucleation-promoting, and further cooling zones.
COMPUTERS & CHEMICAL ENGINEERING
(2021)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Cell & Tissue Engineering
Sara Romanazzo, Chantal Kopecky, Shouyuan Jiang, Riddhesh Doshi, Vipul Mukund, Pallavi Srivastava, Jelena Rnjak-Kovacina, Kilian Kelly, Kristopher A. Kilian
Summary: This study demonstrates how matrix-conjugated hydrogel cell culture materials can normalize a population of induced pluripotent stem cell derived mesenchymal stem cells (iPSC-MSCs) to display a defined secretory profile that promotes enhanced neovascularization. Through a protein-conjugated biomaterials screen, two conditions were identified that promote reproducible secretion of pro-angiogenic and immunomodulatory cytokines. Furthermore, the study shows that secretory activity can be maintained after cryopreservation using defined culture substrates.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2022)
Article
Multidisciplinary Sciences
Mika Ishigaki, Hirofumi Hitomi, Yukihiro Ozaki, Akira Nishiyama
Summary: This study aimed to investigate the intracellular molecular changes during the differentiation of human-induced pluripotent stem cells (iPSCs) into erythropoietin (EPO)-producing cells using Raman spectroscopy and imaging. The results identified three biomarkers characterizing different cell phases and successfully visualized their chemical images in two dimensions. These findings demonstrate the potential application of this method in regenerative medicine for monitoring cell differentiation and discriminating cell maturation at the molecular level.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Review
Biochemistry & Molecular Biology
Tianyuan Shi, Martin Cheung
Summary: Using rodents for modeling neurological diseases has limitations due to differences in genetics, neural development, and physiology between humans and rodents. In the past decade, induced pluripotent stem cells and induced neural stem cells generated through reprogramming somatic cells have offered a powerful alternative for studying disease pathogenesis and testing regenerative medicines. Urine-derived stem cells are an ideal cell source for reprogramming due to their proliferation, multipotency, epithelial nature, and ease of reprogramming.
CELL AND BIOSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yue Su, Ling Wang, Zhiqiang Fan, Ying Liu, Jiaqi Zhu, Deborah Kaback, Julia Oudiz, Tayler Patrick, Siu Pok Yee, Xiuchun (Cindy) Tian, Irina Polejaeva, Young Tang
Summary: This study successfully generated biPSCs with long-term self-renewal capacity and pluripotent characteristics from bovine mesenchymal stem cells. The cells exhibited morphological changes under different culture conditions, and transcriptome analysis showed high similarity to bovine ESCs and embryos. The naive-like biPSCs also demonstrated the ability to integrate into extra-embryonic tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Hong Cao, Qian Zhou, Chungeng Liu, Yecen Zhang, Minghui Xie, Weihua Qiao, Nianguo Dong
Summary: This study investigates the effect of substrate stiffness on the differentiation of human-induced pluripotent stem cells (h-iPSCs) into valve endothelial-like cells (h-iVECs). The results show that moderate substrate stiffness can enhance the differentiation efficiency of h-iVECs and is regulated by the WNT/Calcineurin signaling pathway. This study emphasizes the importance of using substrate stiffness to achieve a more specific and mature differentiation of h-iVECs.
ACTA BIOMATERIALIA
(2022)
Article
Biology
Yu Zhang, Hongyan Liu, Zheng Liu, Ping Long, Xing Zhao, Qi Li, Yuanhua Huang, Yanlin Ma
Summary: A new CryoLogic Vitrification Method (CVM) was developed in this study, loading a small piece of hiPSCs colonies in the vitrification solution to the hook of Fiberplug and immersed directly in a 37 degrees C warming solution for warming. This method achieved high recovery rate and maintained the stemness and differentiation potential of hiPSCs.
Article
Neurosciences
Dwaipayan Adhya, Vivek Swarup, Roland Nagy, Lucia Dutan, Carole Shum, Eva P. Valencia-Alarcon, Kamila Maria Jozwik, Maria Andreina Mendez, Jamie Horder, Eva Loth, Paulina Nowosiad, Irene Lee, David Skuse, Frances A. Flinter, Declan Murphy, Grainne McAlonan, Daniel H. Geschwind, Jack Price, Jason Carroll, Deepak P. Srivastava, Simon Baron-Cohen
Summary: The study suggests that autism-iPSCs diverge from control-iPSCs at a cellular level during early neurodevelopment, indicating unique developmental differences associated with autism may be established at early prenatal stages.
BIOLOGICAL PSYCHIATRY
(2021)
Article
Biochemistry & Molecular Biology
Wang Wang, Ming Yan, Ghazal Aarabi, Ulrike Peters, Marcus Freytag, Martin Gosau, Ralf Smeets, Thomas Beikler
Summary: The study shows that the new cryopreservation method is effective in preserving hDPSCs without compromising their capabilities and stemness. The cells maintain excellent proliferation and differentiation abilities, which are crucial for cell therapy and dental bank establishment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Samantha E. Spellicy, David C. Hess
Summary: Inflammation is a key contributing factor to the pathogenesis of stroke, leading to increased oxidative stress and neuronal cell death. Recent research has shown that induced pluripotent stem cells have the potential to suppress neuroinflammation, mainly by modulating inflammatory signaling and endogenous inflammatory cell populations.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Chia-Hsing Pi, Guanglin Yu, Peter I. Dosa, Allison Hubel
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Chemistry, Multidisciplinary
Guanglin Yu, Rui Li, Allison Hubel
Review
Hematology
Kathlyn Hornberger, Guanglin Yu, David McKenna, Allison Hubel
TRANSFUSION MEDICINE AND HEMOTHERAPY
(2019)
Review
Cell & Tissue Engineering
Rui Li, Rachel Johnson, Guanglin Yu, David H. Mckenna, Allison Hubel
Article
Biophysics
Chia-Hsing Pi, Peter I. Dosa, Allison Hubel
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
(2020)
Article
Biotechnology & Applied Microbiology
Rui Li, Kathlyn Hornberger, James R. Dutton, Allison Hubel
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Cell & Tissue Engineering
Chia-Hsing Pi, Kathlyn Hornberger, Peter Dosa, Allison Hubel
Article
Engineering, Chemical
Kathlyn Hornberger, Rui Li, Ana Rita C. Duarte, Allison Hubel
Summary: This study investigates the potential of Natural deep eutectic systems (NADES) as cryoprotective agents (CPA) for cell preservation, and develops an optimized CPA formulation using trehalose-glycerol NADES (T:G) supplemented with isoleucine. Results show that while the thermophysical properties of CPA solutions alone cannot predict optimal cell survival, the combination of NADES with isoleucine significantly improves cell recovery and growth post-thaw, indicating the importance of stabilizing biological structures for successful cryopreservation.
Article
Cell & Tissue Engineering
Adam Joules, Julianne Connors, Rachel Johnson, Elizabeth A. Van Orsow, David H. McKenna, Sarah Nikiforow, Jerome Ritz, Adrian Gee, Allison Hubel
Summary: Cell therapies are a promising treatment option for various diseases, and standards for most aspects of the cell therapy lifecycle have been established, but there are limited standardized protocols regarding the day-of infusion. Variations were identified in facility layout, product thawing location, and cell therapy lab location, which influenced the time from thaw to infusion. Opportunities for standardizing and streamlining the infusion workflow were identified to facilitate adoption of cell therapies at more hospitals.
Article
Genetics & Heredity
Pierre Comizzoli, Pasqualino Loi, Pasquale Patrizio, Allison Hubel
Summary: Long-term preservation of high-quality sperm, eggs, embryos, and gonadal tissues is crucial for human reproductive medicine and population management. Research suggests that suspending gametes or gonadal tissues in trehalose glass after dehydration and storing them at supra-zero temperatures can be an alternative to freezing.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Cell Biology
Rui Li, Patrick Walsh, Vincent Truong, Ashley Petersen, James R. Dutton, Allison Hubel
Summary: This study investigated the challenges of cryopreservation during hiPSC differentiation to sensory neurons, revealing higher sensitivity to undercooling in hiPSC-derived neuronal cells and higher sensitivity to suboptimal cooling rates in stem cell developmental stages. Functional sensory neurons were successfully obtained following DMSO-free cryopreservation, highlighting the importance of dissociating adherent cultures in cell survival and functionality post-cryopreservation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Engineering, Biomedical
Guanglin Yu, Allison Hubel
REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE
(2019)
Article
Cell Biology
William Schleif, Rita T. Lawlor, Allison Hubel, Zisis Kozlakidis, Marianne K. Henderson
BIOPRESERVATION AND BIOBANKING
(2019)