Review
Cell Biology
Kyu Hwan Kwack, Hyeon-Woo Lee
Summary: Dental caries is a common disease that causes damage to teeth and pulp tissue. Dental pulp stem cells have multipotency, high proliferation rate, and immunosuppressive properties, making them ideal for regenerating damaged pulp tissue.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Mohammed Zayed, Koichiro Iohara, Hideto Watanabe, Mami Ishikawa, Michiyo Tominaga, Misako Nakashima
Summary: The study found that hypoxia-preconditioned DPSCs (hpDPSCs) have advantages in proliferation rate and stemness, making them a potential effective source for pulp regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Multidisciplinary Sciences
Yuyang Chen, Xiayi Wang, Zhuoxuan Wu, Shiyu Jia, Mian Wan
Summary: Dental-derived stem cells, such as DPSCs, SHEDs, SCAPs, DFPCs, and PDLSCs, have excellent proliferation ability and multi-directional differentiation potential. They have significant application prospects in tissue regeneration due to their abundance, biocompatibility, and effectiveness. Epigenetic regulation plays a crucial role in the biological functions of dental-derived stem cells, and their potential for pulp and periodontal regeneration is highly promising.
Review
Biotechnology & Applied Microbiology
Lina Yu, Guanxiong Zhu, Zeyu Zhang, Yang Yu, Liting Zeng, Zidan Xu, Jinlong Weng, Junyi Xia, Jiang Li, Janak L. L. Pathak
Summary: Apoptotic bodies (ApoBDs) are extracellular vesicles released by dying cells that play an important role in intercellular communications and have potential applications in disease diagnosis and treatment. Recent studies have shown that defective clearance of ApoBDs and infected-cells-derived ApoBDs can contribute to certain diseases. ApoBDs have demonstrated immunomodulatory, virus removal, vascular protection, tissue regenerative, and disease diagnostic potential, and can also enhance drug stability and targeted therapy efficacy. This review summarizes the recent advances in ApoBDs-related research and discusses their role in health and diseases, as well as the challenges and prospects of their diagnostic and therapeutic applications.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Immunology
Fenyao Li, Xinxin Wang, Jin Shi, Shuting Wu, Wenbo Xing, Yan He
Summary: Dental pulp stem cells have strong immunomodulatory capabilities and can be used to treat inflammation-related diseases and autoimmune disorders. The mechanism of action is complex and may involve the regulation of immune cells through inflammatory immune-related signaling pathways.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Applied
Davide Porrelli, Martina Gruppuso, Federica Vecchies, Eleonora Marsich, Gianluca Turco
Summary: The study demonstrated that porous alginate/hydroxyapatite scaffolds with a bioactive coating can enhance the healing of bone defects and improve the osteoinductive and osteoconductive properties of the scaffolds. The bioactive coating can enhance the adhesion of human dental pulp stem cells on the scaffolds, while also stimulating the osteogenic differentiation of the cells.
CARBOHYDRATE POLYMERS
(2021)
Review
Pharmacology & Pharmacy
Nan Hu, Weiping Li, Wentao Jiang, Jin Wen, Shensheng Gu
Summary: Dental pulp and periapical diseases cause pain and economic loss for patients. Although root canal therapies provide relief, restoring the nutrition, sensory, and immune functions of dental pulp remains difficult. Tissue engineering and bioactive scaffolds have made significant progress in creating a suitable microenvironment for dental pulp regeneration. This article discusses current challenges and novel perspectives in using bioactive scaffolds to promote dental pulp regeneration.
Review
Cell Biology
Wei Xiong, Ye Liu, Heng Zhou, Shuili Jing, Yan He, Qingsong Ye
Summary: Alzheimer's disease is a destructive neurodegenerative disease with a lack of effective drug candidates. Dental pulp stem cells have the potential for neuronal differentiation and neuroprotection, making them a promising approach for the treatment and protection of damaged neurons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Lihua Luo, Yan He, Ling Jin, Yanni Zhang, Fernando P. Guastaldi, Abdullkhaleg A. Albashari, Fengting Hu, Xiaoyan Wang, Lei Wang, Jian Xiao, Lingli Li, Jianming Wang, Akon Higuchi, Qingsong Ye
Summary: By optimizing the scaffold material synthesis protocol, a third-generation nerve regeneration conduit CSM-GFD was constructed and applied for repairing a 15-mm long defect of the sciatic nerve in a rat model. At histologic and physical levels, the conduit regenerated nerve tissue and achieved functional recovery, showing promising potential as a tissue engineering approach for treating large gap defects in peripheral nerve injuries.
BIOACTIVE MATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Diana B. Sequeira, Ana Rafaela Oliveira, Catarina M. Seabra, Paulo J. Palma, Carlos Ramos, Maria H. Figueiredo, Ana C. Santos, Ana L. Cardoso, Joao Peca, Joao Miguel Santos
Summary: The findings of this study demonstrate that SCAPs combined with PRP scaffold can induce the formation of dentin-like and pulp-like tissues in root segments. The presence of barriers such as ProRoot MTA or Biodentine did not significantly affect the deposition of hard material in the root segments, but the area of newly deposited dentin was significantly greater in segments with a Biodentine barrier compared to a ProRoot MTA barrier.
CLINICAL ORAL INVESTIGATIONS
(2021)
Review
Cell & Tissue Engineering
Liangrui Chen, Shasha Zhu, Shujuan Guo, Weidong Tian
Summary: Periodontitis is a common oral disease that causes damage to both hard and soft tissues of the periodontium, leading to tooth mobility and loss. Mesenchymal stem cells (MSCs) have emerged as a promising therapy for periodontitis due to their unique characteristics. Accumulating evidence suggests that the regenerative effects of MSCs are mainly mediated through paracrine signaling, including the release of extracellular vesicles (EVs) that contain bioactive substances. These MSC-EVs play a crucial role in regulating the biological functions of periodontal tissue cells, immune cells, and the local microenvironment, promoting tissue regeneration and repair. As a cell-free therapy, MSC-EVs offer advantages in terms of stability, immune rejection, and ethical concerns, making them potentially valuable for clinical applications in periodontal regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Biochemistry & Molecular Biology
Sahng G. Kim
Summary: Despite the recent surge in studies on dental pulp regeneration using tissue engineering strategies, the translation of these findings into therapeutic applications remains a challenge. This scoping review systematically reviewed the efficacy of mesenchymal stem cell transplantation for dental pulp regeneration, showing potential for vascularized pulp-like tissue regeneration and successful regeneration of vital tissue in clinical trials. More high-quality studies are needed to further evaluate the safety and efficacy of stem cell transplantation for dental pulp regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Lulu Liu, Lu Qiu, Yaqian Zhu, Lei Luo, Xinpei Han, Mingwu Man, Fuguang Li, Maozhi Ren, Yadi Xing
Summary: Regeneration is the process of repairing and replacing lost tissues and organs in organisms. Stem cells play a crucial role in animal and plant regeneration, with developmental processes involving totipotent, pluripotent, and unipotent stem cells. Stem cells and their products have diverse applications in agriculture, animal husbandry, environmental protection, and regenerative medicine. This review explores the similarities and differences in animal and plant tissue regeneration, as well as the regulation of regeneration through signaling pathways and key genes, aiming to provide insights for practical applications in agriculture and human organ regeneration, and to expand the future application of regeneration technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Qiannan Sun, Zimeng Zhuang, Rushui Bai, Jie Deng, Tianyi Xin, Yunfan Zhang, Qian Li, Bing Han
Summary: Dental pulp stem cells (DPSCs) are ideal seed cells for cartilage tissue engineering due to their easy availability, multi-lineage differentiation ability, and high proliferation ability. This study demonstrates that the histone-modifying enzymes KDM3A and G9A regulate the chondrogenic differentiation of DPSCs by controlling the degradation of SOX9 protein through lysine methylation. KDM3A promotes chondrogenesis by boosting the protein level of SOX9, while G9A hinders chondrogenic differentiation by reducing the protein level of SOX9. The findings provide a theoretical basis to enhance the clinical use of DPSCs in cartilage tissue-engineering therapies.
Review
Biochemistry & Molecular Biology
Zhuo Xie, Zongshan Shen, Peimeng Zhan, Jiayu Yang, Qiting Huang, Shuheng Huang, Lingling Chen, Zhengmei Lin
Summary: Treatment of pulpal and periapical diseases faces challenges, but significant progress has been made in achieving true pulp tissue regeneration through technologies such as stem cells, biomaterials, and growth factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell & Tissue Engineering
Batoul Chouaib, Frederic Cuisinier, Pierre-Yves Collart-Dutilleul
Summary: Conditioned medium (CM) produced from dental mesenchymal stem cells (DMSC-CM) is emerging as a potential alternative to cell therapy. However, there is a lack of standardized and optimized manufacturing procedures due to variable protocols, leading to inconsistent results.
WORLD JOURNAL OF STEM CELLS
(2022)
Article
Pharmacology & Pharmacy
Afreen Jailani, Shanthini Kalimuthu, Vidhyashree Rajasekar, Sumanta Ghosh, Pierre-Yves Collart-Dutilleul, Naveen Fatima, Hyun Koo, Adline Princy Solomon, Frederic Cuisinier, Prasanna Neelakantan
Summary: In this study, a novel platform using porous silicon microparticles loaded with natural compound trans-cinnamaldehyde (TC) was developed to inhibit biofilm formation of dental caries pathogens. The results showed that pSi-TC significantly inhibited biofilm formation, reduced adhesion to the substrate, and hindered acid production. This study highlights the potential of using pSi-TC as a dental caries-preventive material.
Article
Chemistry, Multidisciplinary
Hamideh Salehi, Anuradha Ramoji, Said Mougari, Peggy Merida, Aymeric Neyret, Jurgen Popp, Branka Horvat, Delphine Muriaux, Frederic Cuisinier
Summary: This study demonstrates the use of confocal Raman microspectroscopy to analyze biochemical modifications in cells caused by SARS-CoV-2 infection. The results show a virus-specific Raman molecular signature and highlight the potential of Raman spectroscopy in studying virus-host interactions and developing new COVID-19 biomarkers.
COMMUNICATIONS CHEMISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
A. Desoutter, I. Panayotov, F. Cuisinier, D. Carayon
Summary: This study used high-resolution microcomputed tomography to reveal the three-dimensional structure and characteristics of the tufts in human dental enamel, shedding light on their distribution, dynamics, and the connection between their undulations and the dentin surface. Further research is needed to explore the possible connection between these tufts and Hunter-Schreger bands.
ARCHIVES OF ORAL BIOLOGY
(2022)
Review
Engineering, Biomedical
Mahmoud Sedky Adly, Afnan Sedky Adly, Richard Younes, Marwan El Helou, Ivan Panayotov, Frederic Cuisinier, Delphine Carayon, Elias Estephan
Summary: This review summarizes recent literature on the mechanisms and parameters of pulsed ultrasound used during orthodontic treatments to prevent and repair root resorption. The most effective parameters were found to be a frequency of 1.5 MHz, pulse repetition frequency of 1000 Hz, output intensity of 30 mW/cm2, application duration of 20 min, and a total of 14 sessions with a repetition interval of 1 day. Pulsed ultrasound was found to induce changes in various cellular factors and proteins related to bone remodeling.
EXPERT REVIEW OF MEDICAL DEVICES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Margaux Vignon, Amandine Bastide, Aurore Attina, Alexandre David, Philippe Bousquet, Valerie Orti, Jerome Vialaret, Sylvain Lehmann, Dominique DevilleDe Periere, Christophe Hirtz
Summary: This study used multiplexed mass spectrometry (MS) to analyze the salivary epitranscriptomic profiles as periodontitis biomarkers. Some nucleosides were found to be significantly altered in periodontitis patients, which may serve as promising biomarkers. This research provides new directions and insights for the discovery of diagnostic biomarkers for periodontitis.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Ivan V. Panayotov, Attila G. Vegh, Marta Martin, Boyan Vladimirov, Christian Larroque, Csilla Gergely, Frederic J. G. Cuisinier, Elias Estephan
Summary: The functionalization of titanium and titanium alloys implant surfaces via material-specific peptides influences host/biomaterial interaction. The use of peptides as molecular linkers between cells and implant material to improve keratinocyte adhesion is reported. In vivo and in vitro experiments demonstrate the promising performance of bioengineered peptides in improving epithelial cell adhesion to titanium-based implants.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Batoul Chouaib, Mandana Haack-Sorensen, Franck Chaubron, Frederic Cuisinier, Pierre-Yves Collart-Dutilleul
Summary: Mesenchymal stem cell secretome or conditioned medium (MSC-CM) is a crucial starting point for various derived products and can be used for regenerative medicine. However, issues such as manufacturing protocols and quality control must be addressed before their clinical application. This review highlights the importance of standardized procedures in conditioned medium production and the influence of various factors on the quality of the secretome and its derivatives.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Hematology
Afnan Sedky Adly, Mahmoud Sedky Adly, Frederic Cuisinier, Jean-Christophe Egea, Ivan Panayotov, Aya Sedky Adly, Eve Malthiery
Summary: This study reviews the existing literature and finds that laser irradiation is an effective method for inducing blood coagulation in various medical fields. Different laser parameters are required for different conditions.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2023)
Article
Engineering, Biomedical
Naveen Fatima, Hamideh Salehi, Eduardo J. Cueto-Diaz, Alban Desoutter, Frederic Cuisinier, Frederique Cunin, Pierre-Yves Collart-Dutilleul
Summary: Nanostructured porous silicon is a biocompatible and bioresorbable material with potential applications in tissue engineering as a biomaterial scaffold for bone regeneration. Its resorption kinetics and silicon release have been investigated to understand its behavior in vivo and promote bone formation.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Biotechnology & Applied Microbiology
Matthieu Renaud, Philippe Bousquet, Gerard Macias, Gael Y. Rochefort, Jean-Olivier Durand, Lluis F. Marsal, Frederic Cuisinier, Frederique Cunin, Pierre-Yves Collart-Dutilleul
Summary: Currently, bone regeneration techniques using biomaterials have limited efficiency. Tissue engineering combining biomaterials and stem cells is an important avenue of development to improve bone regeneration. In this study, the use of porous silicon (pSi) particles carrying dental pulp stem cells (DPSC) was found to promote greater bone formation in vivo.
BIOENGINEERING-BASEL
(2023)
Review
Engineering, Biomedical
Sofia Silvia Piglionico, Coline Pons, Olivier Romieu, Frederic Cuisinier, Bernard Levallois, Ivan Vladislavov Panayotov
Summary: Based on the concept of tissue engineering, regenerative endodontics aims to preserve dental pulp vitality or regenerate pulp-like tissue in necrotic root canals. Various in vitro, ex vivo, and in vivo models have been used to improve tissue engineering methods for pulp regeneration. This review explores the evolution of these laboratory models and discusses the challenges in establishing reproducible models.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2023)
Article
Instruments & Instrumentation
Alban Desoutter, Gerard Subsol, Eric Fargier, Alexandre Sorgius, Herve Tassery, Michel Fages, Frederic Cuisinier
Summary: This study provides a simple assessment of the resolution of an IOS by measuring a reference sample and highlights factors that may influence the resolution. The results show a clear loss of information for objects smaller than 100 micrometers. As there is no standardized procedure to evaluate the resolution of IOSs, the method developed in this study can serve as a positive parameter for practitioners to control the performance of IOSs.
METROLOGY AND MEASUREMENT SYSTEMS
(2022)
Article
Nanoscience & Nanotechnology
Habib Belaid, Carole Barou, Pierre-Yves Collart-Dutilleul, Alban Desoutter, Marilyn Kajdan, Florence Bernex, Raphael Tetreau, Frederic Cuisinier, Jonathan Bares, Vincent Huon, Catherine Teyssier, David Cornu, Vincent Cavailles, Mikhael Bechelany
Summary: The aim of this study was to develop an injectable radio-opaque and macroporous calcium phosphate cement (CPC) as a bone substitute for the treatment of pathologic vertebral fractures. The CPC/PLGA exhibited good mechanical properties and biocompatibility, with satisfactory injectability and degradation performance.
ACS APPLIED BIO MATERIALS
(2022)