Article
Biochemistry & Molecular Biology
Dongyun Kim, Hanjun Hwangbo, GeunHyung Kim
Summary: By utilizing a submerged bioprinting process, aligned cell-laden filaments with high viability and gene expression levels were successfully fabricated, showing potential for obtaining bioengineered constructs for various tissues.
Article
Geriatrics & Gerontology
Patricia Sosa, Elena Alcalde-Estevez, Ana Asenjo-Bueno, Patricia Plaza, Natalia Carrillo-Lopez, Gemma Olmos, Susana Lopez-Ongil, Maria Piedad Ruiz-Torres
Summary: Hyperphosphatemia impairs myogenic differentiation and leads to muscle fibrosis, with studies in older mice demonstrating a close relationship between age-related hyperphosphatemia and the decrease in myogenic factors and increase in fibrotic factors.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2021)
Article
Cell Biology
Nasa Xu, Jianbo Wu, Jose L. Ortiz-Vitali, Yong Li, Radbod Darabi
Summary: Recent advancements in reprogramming somatic cells into induced pluripotent stem cells (iPSCs) have allowed for the efficient derivation of skeletal myogenic progenitors from human pluripotent stem cells (ESCs/iPSCs) using a directed differentiation method without the need for gene integration or modification. This method induces mesoderm and subsequently myotomal progenitors, enabling the generation of skeletal myogenic progenitors in a short time frame with high efficiency.
Article
Biochemistry & Molecular Biology
Aiping Lu, Ping Guo, Haiying Pan, Chieh Tseng, Krishna M. Sinha, Fan Yang, Alex Scibetta, Yan Cui, Matthieu Huard, Ling Zhong, Sudheer Ravuri, Johnny Huard
Summary: Research shows that pregnant mice demonstrate accelerated muscle healing, superior muscle regeneration, and reduced inflammation and necrosis following muscle injury. Additionally, MPCs from pregnant mice display improved myogenic differentiation capacity in vitro and muscle regeneration in vivo.
Article
Biology
Shufang Cai, Bin Hu, Xiaoyu Wang, Tongni Liu, Zhuhu Lin, Xian Tong, Rong Xu, Meilin Chen, Tianqi Duo, Qi Zhu, Ziyun Liang, Enru Li, Yaosheng Chen, Jianhao Li, Xiaohong Liu, Delin Mo
Summary: In this study, the molecular and cellular dynamics of pig embryonic myogenesis were studied through single-cell resolution analysis of gene expression and chromatin accessibility. The differentiation trajectory of pig skeletal muscle development was identified, and critical regulators such as EGR1 and RHOB were identified through integrative analysis.
Article
Biochemistry & Molecular Biology
Flavia Carton, Dalila Di Francesco, Luca Fusaro, Emma Zanella, Claudio Apostolo, Francesca Oltolina, Diego Cotella, Maria Prat, Francesca Boccafoschi
Summary: The study evaluated the myogenic potential of dECM substrate derived from decellularized bovine pericardium on murine muscle cells and found that dECM could support and enhance the myogenic potential of muscle cells compared to cells grown on a standard plastic surface. This suggests that extracellular matrix proteins could be a promising therapeutic strategy for skeletal muscle tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Olga G. Jaime, Jessica Arias, Shreya Pavani, April D. Pyle, Michael R. Hicks
Summary: The co-expression of SIX1 and PAX3 is the most indicative factor for skeletal muscle differentiation in human pluripotent stem cells (hPSCs). Manipulating seeding density, monitoring metabolic secretion, and altering the concentration of CHIR99021 can enhance the emergence of SIX1+PAX3+ precursors in hPSC differentiation. Inhibition of non-myogenic lineages can modulate PAX3 expression independently of SIX1.
Article
Chemistry, Multidisciplinary
Inseon Kim, Seunghun S. Lee, Adhideb Ghosh, Stephen J. Ferguson, Ori Bar-Nur
Summary: This study presents an engineered biomimetic muscle construct that can self-regenerate and produce aligned myotubes using induced myogenic progenitor cells. The construct faithfully recapitulates a step-wise myogenic differentiation program and can rapidly differentiate into aligned myotubes within the constructs when subjected to a damaging myonecrotic agent. This work showcases the potential for translational applicability in regenerative medicine.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Mengxun Li, Quan Liu, Su Xie, Chong Fu, Jiaxuan Li, Cheng Tian, Xin Li, Changchun Li
Summary: In this study, it was found that the lncRNA TCONS_00323213 is upregulated during porcine skeletal muscle satellite cell (PSC) differentiation and plays a role in the proliferation and differentiation of PSC. The interaction between TCONS_00323213 and PKNOX2 promotes the expression of MyoG and enhances PSC differentiation. This finding provides insights into the molecular mechanisms by which lncRNAs regulate porcine myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Denis Vecellio Reane, Cristina Cerqua, Sabrina Sacconi, Leonardo Salviati, Eva Trevisson, Anna Raffaello
Summary: Alternative splicing plays a crucial role in skeletal muscle, both during myogenesis and in post-natal life, by regulating the transcripts of contractile proteins, metabolic enzymes, and transcription factors. Aberrations in alternative splicing can lead to pathological conditions. In addition, many skeletal muscle Ca2+ homeostasis genes are also regulated by alternative splicing mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Yonggang Zhang, Pamela Habibovic
Summary: Biochemical signals and biophysical cues both play crucial roles in regulating cellular activities and maintaining tissue and organ function. This article provides an overview of the current state of the art in designing and developing materials and devices for delivering mechanical stimuli to cells and tissues, highlighting challenges and perspectives for future research.
ADVANCED MATERIALS
(2022)
Article
Multidisciplinary Sciences
Junio Dort, Zakaria Orfi, Paul Fabre, Thomas Molina, Talita C. Conte, Karine Greffard, Ornella Pellerito, Jean-Francois Bilodeau, Nicolas A. Dumont
Summary: Resolvin-D2 shows potential as a new therapeutic alternative for Duchenne Muscular Dystrophy, targeting inflammation and myogenic cells to enhance muscle regeneration. In mouse models, Resolvin-D2 is more effective than glucocorticoids in mitigating muscular dystrophy by dampening inflammation and promoting myogenesis.
NATURE COMMUNICATIONS
(2021)
Article
Cell & Tissue Engineering
Congshan Sun, Suraj Kannan, In Young Choi, HoTae Lim, Hao Zhang, Grace S. Chen, Nancy Zhang, Seong-Hyun Park, Carlo Serra, Shama R. Iyer, Thomas E. Lloyd, Richard M. Lovering, Su Bin Lim, Peter Andersen, Kathryn R. Wagner, Gabsang Lee, Chulan Kwon
Summary: Human pluripotent stem cell (hPSC)-derived myogenic progenitor cell (MPC) transplantation can contribute to muscle fiber regeneration in mice, providing a proof of principle for future hPSC-based cell therapy for muscle disorders.
Article
Biochemistry & Molecular Biology
Mai Thi Nguyen, Wan Lee
Summary: This study investigated the role of miR-320-3p in myogenic progenitor cell differentiation and found that miR-320-3p mediates muscle wasting by inhibiting CFL2 expression, activating YAP1, and suppressing myoblast differentiation. These findings provide important insights into muscle myogenesis and maintenance of muscle quality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Aleksandra Ulman, Marta Kot, Klaudia Skrzypek, Barbara Szewczyk, Marcin Majka
Summary: Induced pluripotent stem cells are a useful tool for studying human embryo development processes such as myogenesis due to their ability to differentiate into three germ layers. Different protocols for obtaining myogenic cells have been described, with Protocol III found to be the most efficient. CD56 was also found to not be a specific marker for evaluating myogenic differentiation.
Article
Oncology
Ki Cheon Kim, Madduma Hewage Susara Ruwan Kumara, Kyoung Ah Kang, Mei Jing Piao, Min Chang Oh, Yea Seong Ryu, Jin Oh Jo, Young Sun Mok, Jennifer H. Shin, Yeunsoo Park, Seong Bong Kim, Suk Jae Yoo, Jin Won Hyun
MOLECULAR MEDICINE REPORTS
(2017)
Article
Engineering, Civil
Min-Kyung Jeon, Tae-Hyuk Kwon, Jin-Sung Park, Jennifer H. Shin
GEOMECHANICS AND ENGINEERING
(2017)
Article
Biochemistry & Molecular Biology
Daniel A. Balikov, Spencer W. Crowder, Jung Bok Lee, Yunki Lee, Ung Hyun Ko, Mi-Lan Kang, Won Shik Kim, Jennifer H. Shin, Hak-Joon Sung
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Cell Biology
Youngbin Cho, Minjeong Son, Hyuntae Jeong, Jennifer H. Shin
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Surgery
Joon Seok Lee, Jong Seong Kim, Jeong Woo Lee, Kang Young Choi, Jung Dug Yang, Byung Chae Cho, Eun Jung Oh, Tae Jung Kim, Ung Hyun Ko, Jennifer H. Shin, Sewha Jeon, Yong Jig Lee, Ho Yun Chung
AESTHETIC PLASTIC SURGERY
(2019)
Article
Engineering, Biomedical
In Gul Kim, Mintai P. Hwang, Jin Sil Park, Su-Hyun Kim, Jung-Hyun Kim, Hyo Jin Kang, Ramesh Subbiah, Ung Hyun Ko, Jennifer H. Shin, Chong-Hyun Kim, Donghoon Choi, Kwideok Park
ADVANCED HEALTHCARE MATERIALS
(2019)
Article
Multidisciplinary Sciences
Ung Hyun Ko, Jongjin Choi, Jinseung Choung, Sunghwan Moon, Jennifer H. Shin
SCIENTIFIC REPORTS
(2019)
Article
Multidisciplinary Sciences
Hwanseok Jang, Jongseong Kim, Jennifer H. Shin, Jeffrey J. Fredberg, Chan Young Park, Yongdoo Park
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Chemistry, Analytical
Jin-Sung Park, Geunseob Oh, Jiwon Kim, Eun Young Park, Jennifer H. Shin
ANALYTICAL SCIENCES
(2019)
Article
Materials Science, Biomaterials
Seung Jung Yu, Goro Choi, Youngbin Cho, Minseok Lee, Younghak Cho, Jennifer H. Shin, Eunjung Lee, Sung Gap Im
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Article
Biochemical Research Methods
Hwanseok Jang, Jongseong Kim, Jennifer H. Shin, Jeffrey J. Fredberg, Chan Young Park, Yongdoo Park
Meeting Abstract
Biochemistry & Molecular Biology
Ung Hyun Ko, Jongjin Choi, Jinseung Choung, Sunghwan Moon, Jennifer Hyunjong Shin
Meeting Abstract
Biochemistry & Molecular Biology
Youngbin Cho, Minjeong Son, Ung Hyun Ko, Hyuntae Jeong, Jennifer Hyunjong Shin
Article
Chemistry, Physical
Jaehyun Lee, Sangyeon Hwang, Dae-Hyun Cho, Jungwoo Hong, Jennifer H. Shin, Doyoung Byun
APPLIED SURFACE SCIENCE
(2017)