Review
Biotechnology & Applied Microbiology
Yanxi Liu, Karan M. Shah, Jian Luo
Summary: Articular cartilage is avascular and has limited ability to repair itself, leading to degenerative joint diseases. Current clinical treatments often result in mechanically inferior cartilage. Understanding factors affecting cartilage homeostasis and exploring emerging regenerative approaches are crucial for future treatment options.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Endocrinology & Metabolism
Weirong Xing, Sheila Pourteymoor, Yian Chen, Subburaman Mohan
Summary: Studies show that targeted deletion of the Cldn12 gene in mice increases articular cartilage, potentially by promoting articular chondrocyte phenotype.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biology
Fan Zhang, Yuanyuan Wang, Ying Zhao, Manqi Wang, Bin Zhou, Xianpeng Ge, Di Chen
Summary: NFATc1 is a crucial regulator of articular chondrocyte differentiation, expressed in the early stage of joint development. It negatively regulates the transcriptional activity of the COL2A1 gene in articular cartilage progenitors, determining the differentiation of articular chondrocytes. These findings highlight the important role of NFATc1 in articular cartilage development.
Article
Cell & Tissue Engineering
Kaiwen Zheng, Yiyang Ma, Cheng Chiu, Yidan Pang, Junjie Gao, Changqing Zhang, Dajiang Du
Summary: The study showed that direct co-culture of rat costal chondrocytes and human Wharton's jelly mesenchymal stem cells can effectively promote chondrogenesis and reduce hypertrophic tendency, demonstrating promising results both in vitro and in vivo experiments.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Nanoscience & Nanotechnology
Zhiyao Liao, Liwei Fu, Pinxue Li, Jiang Wu, Xun Yuan, Chao Ning, Zhengang Ding, Xiang Sui, Shuyun Liu, Quanyi Guo
Summary: A bioactive multifunctional scaffold was developed using Mg2+ and aptamer Apt19S to promote articular cartilage regeneration and early inflammatory regulation, offering a promising strategy for AC defect treatment.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Xiang Li, Shiao Li, Jiatian Qian, Yancheng Chen, Yiqin Zhou, Peiliang Fu
Summary: The study demonstrated that type I collagen-based MACT is an effective treatment for improving functionality and pain levels. Histological evidence confirmed hyaline cartilage induction and showed that repaired cartilage tended to emerge from the deep to the superficial layer. The quantitative MRI T2 mapping test indicated that there still was a difference between the transplanted cartilage and the surrounding hyaline cartilage.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Po-Chih Shen, Cheng-Chang Lu, Shih-Hsiang Chou, Zi-Miao Liu, Shu-Jem Su, Yin-Chun Tien
Summary: This study compared stratified chondrocyte sheets (S-CS) and heterogeneous chondrocyte sheets (H-CS) in terms of expression and therapeutic effects, revealing that the S-CS group exhibited higher cell viability, proliferation rate, expression of chondrogenic markers, secretion of tissue inhibitor of metalloproteinase, and GAG production level. The mini-pigs articular cartilage defect model showed that the S-CS group had a higher International Cartilage Repair Society (ICRS) macroscopic score and displayed a zonal structure closer to native cartilage.
Review
Biochemistry & Molecular Biology
Darina Bacenkova, Marianna Trebunova, Jana Demeterova, Jozef Zivcak
Summary: Hyaline cartilage lacks self-repair potential, but tissue engineering methods can be used to address the issue of cartilage defect repair. Research shows that chondrocytes and mesenchymal stromal cells have favorable repair abilities in a three-dimensional culture environment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Zelong Dou, Daniel Muder, Marta Baroncelli, Ameya Bendre, Alexandra Gkourogianni, Lars Ottosson, Torbjorn Vedung, Ola Nilsson
Summary: The study found that perichondrium and periosteum transplanted into articular cartilage defects can be transformed into cartilaginous articular surfaces. Perichondrium transplants developed into an articular-like, hyaline cartilage, while periosteum transplants produced a less resilient fibro-cartilage.
Review
Medicine, Research & Experimental
Weichang Xu, Jing Zhu, Jiawei Hu, Lin Xiao
Summary: Tissue engineering holds great promise in cartilage injury repair and replacement. However, issues such as immunogenicity, stability, and mechanical strength have remained unsolved in articular cartilage tissue engineering. The lack of replicating the chondrocyte biomechanical microenvironment (BME) has been a notable factor. Recent studies have highlighted the crucial role of BME in chondrocyte phenotype and cartilage functions, leading to more precise research in engineering the chondrocyte BME for articular cartilage tissue engineering. This review discusses the effects of the chondrocyte BME and explores the strategies for engineering it in articular cartilage tissue engineering, with a focus on simulating key characteristics and providing dynamic mechanical stimulation.
Review
Biochemistry & Molecular Biology
Zhengjie Zhou, Jingtong Zheng, Xiaoting Meng, Fang Wang
Summary: There is increasing evidence that chondrocytes within articular cartilage are affected by endogenous force-related electrical potentials. Furthermore, electrical stimulation (ES) promotes the proliferation of chondrocytes and the synthesis of extracellular matrix (ECM) molecules, which accelerate the healing of cartilage defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Carlos Martinez-Armenta, Carlos Suarez-Ahedo, Anell Olivos-Meza, Maria C. Camacho-Rea, Laura E. Martinez-Gomez, Guadalupe Elizabeth Jimenez-Gutierrez, Gabriela A. Martinez-Nava, Luis E. Gomez-Quiroz, Carlos Pineda, Alberto Lopez-Reyes
Summary: In this study, a scaffold-free 3D culture system was developed to generate spheroids from human chondrocytes. The results showed that spheroids developed in a hypoxic environment exhibited better chondrogenesis characteristics compared to those developed under normoxic conditions. This novel culture system could be a valuable tool for cartilage biology research.
Article
Biochemical Research Methods
Dmitriy A. Nazarov, Georgy M. Denisenko, Gleb S. Budylin, Elizaveta A. Kozlova, Marina M. Lipina, Vladimir A. Lazarev, Evgeny A. Shirshin, Mikhail K. Tarabrin
Summary: Studies have shown that exploring the optical properties of biological tissues in the infrared range can be beneficial for diagnostic tasks. The short wavelength infrared region II (SWIR II) has not been sufficiently studied for diagnostic problems. A Cr2+:ZnSe laser with tuning capability was developed to investigate the possibilities in this region. The study demonstrated that analyzing water and collagen content in samples using diffuse reflectance spectroscopy can be correlated with the optical density spectra. This indicates the potential for using this spectral range to develop diagnostic methods for degenerative diseases like osteoarthritis.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Orthopedics
Zheng Wang, Bin Liu, Kaifeng Lin, Chunguang Duan, Chunmei Wang
Summary: The presence and change of nerve fibers and neuropeptide during early development of articular cartilage in neonatal rats were investigated. The results showed that nerve fibers exist in the articular cartilage matrix and form synaptic contacts with chondrocytes. The neuropeptides SP and NPY significantly increase in articular cartilage during this period.
JOURNAL OF ORTHOPAEDIC SURGERY AND RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Susumu Takemura, Yoichi Ohta, Ryo Sugama, Yukihide Minoda, Yohei Ohyama, Hiroaki Nakamura
Summary: This study found that eldecalcitol can upregulate articular cartilage markers in superficial zone chondrocytes (SZC) and suppress differentiation markers in deep zone chondrocytes (DZC). This regulation may have potential efficacy in inhibiting the progression of osteoarthritis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)