Article
Genetics & Heredity
Alma Kokhmetova, Nagenahalli Dharmegowda Rathan, Deepmala Sehgal, Angelina Malysheva, Madina Kumarbayeva, Makpal Nurzhuma, Ardak Bolatbekova, Gopalareddy Krishnappa, Elena Gultyaeva, Asia Kokhmetova, Zhenis Keishilov, Kanat Bakhytuly
Summary: In this study, two RIL populations were used to detect novel genomic regions associated with rust resistance. Stable QTLs were identified for yellow rust (YR) and leaf rust (LR) resistance, and candidate genes involved in host response regulation were identified. The findings provide valuable information for marker-assisted selection in breeding rust-resistant varieties.
FRONTIERS IN GENETICS
(2023)
Article
Biochemistry & Molecular Biology
Yunlong Pang, Chunxia Liu, Meng Lin, Fei Ni, Wenhui Li, Jin Cai, Ziliang Zhang, Huaqiang Zhu, Jingxian Liu, Jiajie Wu, Guihua Bai, Shubing Liu
Summary: In this study, three QTLs for adult-plant resistance to stripe rust in wheat were identified, and a candidate gene for one of the QTLs was further located. The findings provide favorable genes and markers for breeding programs targeting stripe rust resistance in wheat.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
James A. Kolmer, Matthew N. Rouse
Summary: In this study, a winter wheat line with adult plant resistance to leaf rust was crossed with a susceptible spring wheat to develop recombinant inbred lines. The lines were assessed for leaf rust severity and genotyped for SNP markers to identify QTL associated with resistance. KASP markers were developed for the closely linked SNPs to facilitate marker-based selection of leaf rust resistance in breeding programs.
Article
Plant Sciences
Kali M. Brandt, Xianming Chen, Javier F. Tabima, Deven R. See, Kelly J. Vining, Robert S. Zemetra
Summary: Stripe rust, a worldwide wheat disease caused by Puccinia striiformis f. sp. tritici, results in devastating crop losses, but can be controlled through combining multiple resistance genes in new cultivars. A study identified two resistance QTLs on chromosomes 3B and 3D in winter wheat cultivars, showing their importance in future breeding efforts.
Article
Agronomy
Yahya Rauf, Caixia Lan, Mandeep Randhawa, Ravi P. Singh, Julio Huerta-Espino, James A. Anderson
Summary: The spring wheat line 'Copio' shows high level of resistance to leaf rust pathogen. Seven QTL for resistance to leaf rust were identified in 'Copio', with three QTL consistently expressed in all environments. Additionally, several recombinant inbred lines carrying a combination of resistance alleles were identified, which significantly reduced leaf rust infection.
Article
Agronomy
Yue Zhou, Pu Gao, Xiao-yan Miao, Takele-Weldu Gebrewahid, Yue Jiao, Pei-pei Zhang, Liu-qing Cao, Xin-le Zhang, Zai-feng Li
Summary: This study mapped quantitative trait loci (QTL) for leaf rust and stripe rust resistance using recombinant inbred lines (RILs) derived from the cross between wheat line L224-3 and Zhengzhou 5389. Four QTLs were identified, including a potentially novel QTL derived from the susceptible parent Zhengzhou 5389, a QTL possibly associated with known resistant gene Lr37/Yr17, a potential new locus for rust resistance, and a possible new QTL. These QTLs and their flanking markers could be utilized for candidate gene mining and marker-assisted selection in wheat breeding programs for rust resistance.
Article
Agronomy
Rohit Mago, Chunhong Chen, Xiaodi Xia, Alex Whan, Kerrie Forrest, Bhoja R. Basnet, Geetha Perera, Sutha Chandramohan, Mandeep Randhawa, Matthew Hayden, Urmil Bansal, Julio Huerta-Espino, Ravi P. Singh, Harbans Bariana, Evans Lagudah
Summary: The adult plant stem rust resistance gene Sr63 was identified in durum wheat Glossy Huguenot and molecular markers were developed for its detection. QTL for stem rust resistance were found on chromosomes 1B and 2A, and combinations of these QTL were effective in reducing stem rust severity.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Plant Sciences
Chen Dang, Junli Zhang, Jorge Dubcovsky
Summary: In this study, the high-resolution map of the wheat rust resistance gene Yr78 was generated, and the candidate region associated with this gene was identified. The HI haplotype associated with Yr78 was found in common wheat cultivars, and two molecular markers for this haplotype were developed for the deployment of Yr78 in wheat breeding programs.
Article
Agronomy
Sandra Rollar, Albrecht Serfling, Manuel Geyer, Lorenz Hartl, Volker Mohler, Frank Ordon
Summary: The Bavarian MAGIC Wheat population was used to map QTL for leaf rust resistance, revealing 19 QTL, with 6 potentially being new QTL. The strategy of using multiparent advanced generation intercross (MAGIC) populations for wheat breeding is significant for developing resistant varieties to combat wheat pathogens.
THEORETICAL AND APPLIED GENETICS
(2021)
Article
Genetics & Heredity
Muhammad Massub Tehseen, Fatma Aykut Tonk, Muzaffer Tosun, Harpinder Singh Randhawa, Ezgi Kurtulus, Izzet Ozseven, Behyan Akin, Ozge Nur Zulfuagaoglu, Kumarse Nazari
Summary: This study identified multiple QTLs controlling stripe rust resistance in wheat using an elite line with adult plant resistance. SNP markers flanking the QTL regions can aid in marker-assisted selection.
FRONTIERS IN GENETICS
(2022)
Article
Plant Sciences
Demei Liu, Chan Yuan, Ravi P. P. Singh, Mandeep S. S. Randhawa, Sridhar Bhavani, Uttam Kumar, Julio Huerta-Espino, Evans Lagudah, Caixia Lan
Summary: Developing wheat varieties with durable resistance is a key objective for breeding programs worldwide. This study identified genetic basis and resistance loci for stripe rust (YR) and leaf rust (LR) in wheat, and developed molecular markers for accelerated breeding.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Jingwei Zhou, Ravi P. Singh, Yong Ren, Bin Bai, Zhikang Li, Chan Yuan, Shunda Li, Julio Huerta-Espino, Demei Liu, Caixia Lan
Summary: The characterization of leaf rust and stripe rust resistance genes in wheat has identified key quantitative trait loci (QTLs) for resistance, including new loci such as QLr.hzau-3BL and QYr.hzau5AL. This provides important insights for wheat breeding and disease management.
Article
Plant Sciences
J. A. Kolmer, M. K. Turner, M. N. Rouse, J. A. Anderson
Summary: This study identified the chromosome locations of leaf rust resistance genes in the hard red spring wheat cultivar AC Taber, with significant QTL effects for resistance found on chromosomes 2BS and 3BS. The genes on these chromosomes were found to be associated with adult plant resistance genes Lr13 and Lr74. Newly developed KASP PCR markers linked to these regions can be used for marker-based selection of these resistance genes.
Article
Agronomy
Mei Deng, Li Long, Yukun Cheng, Fangjie Yao, Fangnian Guan, Yuqi Wang, Hao Li, Zhien Pu, Wei Li, Qiantao Jiang, Yuming Wei, Jian Ma, Houyang Kang, Pengfei Qi, Jirui Wang, Youliang Zheng, Yunfeng Jiang, Guoyue Chen
Summary: In this study, three quantitative trait loci (QTL) associated with adult-plant stripe rust resistance were identified from the Chinese wheat landrace Yibinzhuermai. Among them, QYr.YBZR-6AL and QYr.YBZR-7DS had major effects in different field environments. Furthermore, haplotype analysis revealed that the resistance allele at QYr.YBZR-6AL was present in a small proportion of Chinese wheat landraces.
Article
Plant Sciences
Mathieu Deblieck, Frank Ordon, Albrecht Serfling
Summary: Wheat leaf rust is a significant fungal disease of wheat, and its control measures often break down when the virulence pattern of the fungus changes. In contrast, einkorn wheat shows prehaustorial resistance, which is not dependent on the specific race of the pathogen. By analyzing the genetic population of einkorn wheat, a major QTL associated with resistance was identified, and a specific gene involved in a hypersensitive defense reaction was found within this QTL interval.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
J. A. Kolmer, Z. Su, A. Bernardo, G. Bai, S. Chao
Article
Plant Sciences
J. A. Kolmer
Article
Plant Sciences
J. A. Kolmer, M. E. Ordonez, S. German, A. Morgounov, Z. Pretorius, B. Visser, H. Goyeau, Y. Anikster, M. Acevedo
Article
Plant Sciences
Weizhen Liu, James Kolmer, Sheri Rynearson, Xianming Chen, Liangliang Gao, James A. Anderson, M. Kathryn Turner, Michael Pumphrey
Article
Agronomy
J. A. Kolmer, A. Bernardo, G. Bai, M. J. Hayden, J. A. Anderson
THEORETICAL AND APPLIED GENETICS
(2019)
Article
Plant Sciences
Meriem Aoun, James A. Kolmer, Matthew Breiland, Jonathan Richards, Robert S. Brueggeman, Les J. Szabo, Maricelis Acevedo
Article
Ecology
J. A. Kolmer, A. Herman, M. E. Ordonez, S. German, A. Morgounov, Z. Pretorius, B. Visser, Y. Anikster, M. Acevedo
Article
Plant Sciences
J. A. Kolmer, M. K. Turner, M. N. Rouse, J. A. Anderson
Summary: This study identified the chromosome locations of leaf rust resistance genes in the hard red spring wheat cultivar AC Taber, with significant QTL effects for resistance found on chromosomes 2BS and 3BS. The genes on these chromosomes were found to be associated with adult plant resistance genes Lr13 and Lr74. Newly developed KASP PCR markers linked to these regions can be used for marker-based selection of these resistance genes.
Article
Plant Sciences
J. A. Kolmer, A. Herman, J. P. Fellers
Summary: This study genotyped collections of P. triticina from 2011 to 2018 in the United States, identifying eight common genotype groups and two new genotype groups. The study also detected single nucleotide polymorphisms associated with resistance genes.
Article
Plant Sciences
James A. Kolmer, Oluseyi Fajolu
Summary: Collections of wheat leaves infected with Puccinia triticina obtained from different regions in the United States were analyzed to determine the prevalent virulence phenotypes. The results showed variations in phenotypes and the frequencies of isolates with virulence to different leaf rust resistance genes across different wheat-growing regions. The selection of virulence phenotypes by different Lr genes and factors such as clonal reproduction, overwintering, and limited migration have contributed to the maintenance of diverse P. triticina populations in the United States.
Article
Plant Sciences
James A. Kolmer, Matthew N. Rouse
Summary: In this study, a winter wheat line with adult plant resistance to leaf rust was crossed with a susceptible spring wheat to develop recombinant inbred lines. The lines were assessed for leaf rust severity and genotyped for SNP markers to identify QTL associated with resistance. KASP markers were developed for the closely linked SNPs to facilitate marker-based selection of leaf rust resistance in breeding programs.
Article
Biochemistry & Molecular Biology
Yunlong Pang, Chunxia Liu, Meng Lin, Fei Ni, Wenhui Li, Jin Cai, Ziliang Zhang, Huaqiang Zhu, Jingxian Liu, Jiajie Wu, Guihua Bai, Shubing Liu
Summary: In this study, three QTLs for adult-plant resistance to stripe rust in wheat were identified, and a candidate gene for one of the QTLs was further located. The findings provide favorable genes and markers for breeding programs targeting stripe rust resistance in wheat.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Shivreet Kaur, Taylor Pennington, Emily J. Conley, Andrew Green, James Kolmer, James Anderson, Rajeev Gupta, Upinder Gill
Summary: Deploying adult plant resistance (APR) is important for wheat-breeding programs to combat rust diseases. In this study, a high-resolution melting (HRM)-based diagnostic assay was developed for the Lr34 gene, which is a widely deployed and effective resistance gene against leaf rust fungus Puccinia triticina in wheat. The HRM assay showed high reproducibility in distinguishing between the susceptible and resistant cultivars, as well as differentiating between individual and combination copies of Lr34. While the HRM assay performed well in genotyping breeding lines, it was unable to detect heterozygotes.
Article
Agronomy
J. A. Kolmer, P. Bajgain, M. N. Rouse, J. Li, P. Zhang
Summary: In this study, the leaf rust resistance gene LrX in Thatcher wheat derivative 78-1 was mapped to the distal region of chromosome arm 1DS through hybridization and genotyping. It was found to be closely linked with Lr21, Lr42, and Lr60. Cytological examination of RL6149 showed no evidence of chromosome segment transfer from A- or B-genome to chromosome 1D.
THEORETICAL AND APPLIED GENETICS
(2023)
Article
Agronomy
Mehmet Dogan, Zhen Wang, Mustafa Cerit, Jorge L. L. Valenzuela-Antelo, Smit Dhakal, Chenggen Chu, Qingwu Xue, Amir M. H. Ibrahim, Jackie C. C. Rudd, Amy Bernardo, Paul St. Amand, Guihua Bai, Hongbin Zhang, Shuyu Liu
Summary: Genetic dissection of complex traits in wheat using QTL analysis identified 30 QTL regions associated with yield, end-use quality traits, and agronomic traits. Some of these QTLs were consistent across different chromosomes and showed pleiotropic effects. Three novel QTLs were discovered, along with three major QTLs in colocalization with known genes. Epistasis was also detected. This study provides valuable information for wheat breeding programs through marker-assisted selection.
