Article
Biochemistry & Molecular Biology
Ramesh Kumar Saini, Ji-Woo Yu, Min-Ho Song, Hui-Yeon Ahn, Jung-Hoon Lee, Young-Soo Keum, Ji-Ho Lee
Summary: This research provides information on the contents and composition of important lipophilic constituents in leaf mustard, including carotenoids, tocols, phytosterols, and fatty acids. Among the four cultivars studied, Cheong had the highest levels of carotenoids and tocols, while Red frill contained the most n-3 PUFAs and antioxidants.
Article
Chemistry, Physical
Heber P. Cornelio-Santiago, Renata B. Bodini, Monica R. Mazalli, Cintia Bernardo Goncalves, Christianne E. C. Rodrigues, Alessandra Lopes de Oliveira
Summary: The aim of this study was to evaluate the effects of static time, contact time, and temperature on oil extraction yield, free fatty acids, total phenolic content, and beta-sitosterol content. The results showed that static time and temperature influenced extraction yield and total phenolic content, while beta-sitosterol content and fatty acids were not significantly affected.
JOURNAL OF SUPERCRITICAL FLUIDS
(2022)
Article
Agriculture, Multidisciplinary
Ge Bai, Chuan-Guo Ma, Xiao-Wei Chen
Summary: This study found that free fatty acids are the major provider in the esterification of phytosterols during deodorization process, with temperature and unsaturation playing a significant role. Saturated fatty acids and deodorization process with steam as stripping gas are beneficial in esterifying free phytosterols to phytosterol fatty acid esters.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Food Science & Technology
K. A. Woodling, P. Chitranshi, C. C. Jacob, L. Loukotkova, L. S. Von Tungeln, G. R. Olson, R. E. Patton, S. Francke, S. R. Mog, R. P. Felton, F. A. Beland, Y. Zang, G. Gamboa da Costa
Summary: This study found that long-term consumption of BVO may lead to thyroid follicular cell hypertrophy and changes in serum thyroid-related hormone levels. Additionally, a continuous accumulation of bromide was observed in the heart, liver, and inguinal fat, indicating potential toxic reactions.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Chemistry, Applied
Qin Guo, Tian Li, Yang Qu, Xinping Wang, Li Liu, Hongzhi Liu, Qiang Wang
Summary: The study revealed that plant sterols can effectively inhibit the formation of trans fatty acids in peanut oil, mainly due to their hydroxyl site activities. The differences in FMOE and BDE of plant sterol molecules are closely related to the rate of inhibition of isomerization.
Article
Horticulture
Elise Sipeniece, Inga Misina, Anna Grygier, Ying Qian, Magdalena Rudzinska, Dalija Seglina, Aleksander Siger, Pawel Gornas, Edite Kaufmane
Summary: The study investigated the impact of different harvest years on the seed oil content and composition of Japanese quince, finding significant effects of harvest year and cultivar on oil content and chemical composition. Main fatty acid levels were closely related to temperature in August, while squalene and phytosterols concentrations appeared strongly linked in subsequent harvest years for each cultivar.
SCIENTIA HORTICULTURAE
(2021)
Article
Food Science & Technology
Ge Bai, Chuan-Guo Ma, Xiao-Wei Chen, Lu-Lu Jing, Ya-Peng Yan
Summary: The study investigates the physical migration and chemical conversion of phytosterols during the alkali neutralisation process of corn oil, attributing the decrease in phytosterol content to migration to the soapstock and slight chemical conversions in the heterogeneous system. Additionally, the migration of phytosterols was found to be positively correlated with water content, possibly due to hydrogen bonding between free phytosterols and water. The research provides important insights for retaining phytosterols in the development of healthier and more nutritious edible oils.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Agricultural Engineering
G. Shailaja, T. Bhavya, K. Sandeepa, K. N. Prasanna Rani, T. Prathap Kumar
Summary: This study proposes a simple method for removing free fatty acids from unrefined vegetable oils using reactive extraction with monoethanolamine in methanol. The study investigates the kinetic parameters and establishes the reaction regime for the extraction process. The results show that reactive extraction is highly effective in reducing the free fatty acid content in jatropha oil. Biodiesel prepared from the deacidified oil meets the ASTM standards.
BIOMASS & BIOENERGY
(2023)
Article
Chemistry, Physical
Ivan Dominguez-Candela, Alejandro Lerma-Canto, Salvador Cayetano Cardona, Jaime Lora, Vicent Fombuena
Summary: In this study, a novel epoxidized vegetable oil from chia seed oil (CSO) has been obtained for use in the polymeric industry. The effects of key parameters on the epoxidation process were evaluated, and the best conditions were found to be at 75 degrees C and H2O2:DB (1.50:1).
Article
Chemistry, Applied
Carolina Medeiros Vicentini-Polette, Paulo Rodolfo Ramos, Cintia Bernardo Goncalves, Alessandra Lopes De Oliveira
Summary: This study analyzed the total acidity, fatty acids profile, and free fatty acids in sunflower and soybean oils obtained through green processes. Gas chromatography-mass spectrometry and titrimetric methods were used to determine the primary fatty acids responsible for product acidity. The research provided significant insights into the impact of main fatty acids on acidity in oils obtained by green processes.
Article
Biotechnology & Applied Microbiology
Alejandro Gonzalez-Benjumea, Gisela Marques, Owik M. Herold-Majumdar, Jan Kiebist, Katrin Scheibner, Jose C. del Rio, Angel T. Martinez, Ana Gutierrez
Summary: Enzymatic conversion of vegetable oils into epoxide derivatives shows potential for industrial applications, with different peroxygenases demonstrating varying oxygenation selectivities. The enzymes exhibit high selectivity towards epoxidation, forming pure epoxides, hydroxylated derivatives, and triepoxides depending on the specific fatty acids present in the oils. This environmentally friendly approach offers promising opportunities for the production of reactive fatty-acid epoxides.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Nurhamizah Nordin, Mohd Hafiz Ismail, Muhammad Zulhilmi Ramlee, Mohd Azlien A. Jalil, Fu-Siong Julius Yong, Yi Wang, Nadiah Sidek, Misni Misran, Ninie Suhana Abdul Manan, Poh Wai Chia
Summary: This study proposes a simple and effective method to prepare carboxylic acids from benzaldehydes using betainefatty acids ionic liquid ([Bet][FA] IL) derived from vegetable oil, without the use of chemical oxidants. The [Bet][FA] IL itself is found to be catalytically active and the desired product can be obtained with a good yield using the precipitation method. [Bet][FA] IL has a melting point below 100 degrees C and is thermally stable at high temperatures. This environment-friendly approach supports the use of sustainable catalysts and is expected to be beneficial in future production of chemical intermediates via oxidant-free methods.
Article
Energy & Fuels
Gabriela Polmann, Gerson Lopes Teixeira, Pedro Henrique Santos, Gerardo Alvarez Rivera, Elena Ibanez, Alejandro Cifuentes, Sandra Regina Salvador Ferreira, Jane Mara Block
Summary: The combination of hydraulic pressing and supercritical fluid extraction techniques was effective in maximizing the oil recovery from gurgueia nut. The resulting oil contained high levels of β-sitosterol, γ- and β-tocopherol, and exhibited significant antioxidant potential. This high-quality oil has potential applications in the food, cosmetic, and pharmaceutical industries.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Thermodynamics
Mychelle L. Andrade, Cristina F. Cavalcanti, Dayene L. L. Dantas, Tulio N. Silvestre, Neide Queiroz, Vicente C. Oliveira Costa, Antonia L. Souza
Summary: The blends prepared in this study had a healthier fatty acid composition and higher oxidative stability compared to pure palm oil. The amount of palm oil used in the blends did not affect the formation of trans fatty acids.
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
(2022)
Article
Biochemistry & Molecular Biology
Audrey Cassen, Jean-Francois Fabre, Eric Lacroux, Muriel Cerny, Guadalupe Vaca-Medina, Zephirin Mouloungui, Othmane Merah, Romain Valentin
Summary: An aqueous integrated process was developed to obtain valuable products from sunflower seeds. The process involves high-shear rate crushing, high-pressure homogenization, and centrifugation to extract cream phase rich in lipids and proteins. The addition of an enzymatic step allows for complete hydrolysis of triglycerides. The process offers potential for the valorization of the aqueous phases and pellets in food or technical applications.
Article
Chemistry, Analytical
E. M. Alghamdi, M. J. Whitcombe, S. A. Piletsky, E. V. Piletska
ANALYTICAL METHODS
(2018)
Article
Chemistry, Medicinal
Eman M. Alghamdi, Zahra M. Alamshany, Mohamed A. El Hamd, Ehab S. Taher, Mohammed Farrag El-Behairy, Philip L. Norcott, Adel A. Marzouk
Summary: A new series of tetrasubstituted imidazole derivatives carrying pyrimidine sulfonamide pharmacophores were synthesized and evaluated for their anticancer activities. In-vitro screening showed significant growth inhibition of up to 95% against a full 60-cell-line panel at a single dose of 10 μM. The most active compound exhibited in-vitro anticancer activities against abnormal HER2 and two mutants for EGFR. Apoptotic gene expression analysis revealed that lead compounds induced apoptosis in MCF-7 cell line and caused significant changes in the Bax/Bcl-2 expression ratio. One lead compound led to significant cell-cycle S-phase arrest, while another blocked the cell cycle at G1/S-phase causing the accumulation of cells. Docking analysis showed that these two hybrids had higher binding affinity and interaction with the active side pocket of HER2, L858R, and T790 M compared to co-crystallized ligands. Modeling simulation was consistent with the experimental evaluation.
Article
Biochemistry & Molecular Biology
Taghreed Alsufyani, Najwa Al-Otaibi, Noura J. Alotaibi, Nour Houda M'sakni, Eman M. Alghamdi
Summary: Ten endosymbiotic bacteria from aphids, aphid predators and ants were isolated. The study found that three of these bacterial strains exhibited strong antibacterial activity and showed cytotoxicity against various cancer cell lines.
Article
Entomology
Noura J. J. Alotaibi, Taghreed Alsufyani, Nour Houda M'sakni, Mona A. A. Almalki, Eman M. M. Alghamdi, Dieter Spiteller
Summary: A new method using chemical profiles to identify aphid species has been developed. This method is faster and less laborious compared to traditional DNA analysis.
Article
Chemistry, Multidisciplinary
Zahra M. Alamshany, Eman M. Algamdi, Ismail M. M. Othman, Manal M. Anwar, Eman S. Nossier
Summary: This work focuses on the design and synthesis of a new set of derivatives with pyrazolo[3,4-b]pyridine, pyrazolo[3,4-b]thieno[3,2-e]pyridine, and pyrazolo[3,4-d]thiazole-5-thione scaffolds. The compounds were evaluated for their antiproliferative activity against human cancer cell lines and showed promising cytotoxicity and selectivity towards c-Met.
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).
