Journal
TALANTA
Volume 189, Issue -, Pages 308-314Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.07.012
Keywords
Potentilla; Polyphenols; Tannins, Vibrational spectroscopy; Quantitative analysis; Multivariate analysis
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Funding
- Wroclaw Centre of Biotechnology, program The Leading National Research Centre (KNOW)
- Wroclaw Medical University [ST.D110.17.027]
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The most important active compounds present in Potentilla tormentilla rhizomes were quantitatively determined on the basis of Raman, attenuated total reflection (ATR) and diffuse reflectance mid- and near-infrared spectra (DRIFTS) collected for the untreated plant material. Partial least-squares (PIS) models were constructed utilizing vibrational spectra and the results of reference high-performance liquid chromatography analyses. Applying Raman spectroscopy, total polyphenols, tannins, ellagitannins, procyanidins, agrimoniin, 3-O-galloylquininc acid and catechin were simultaneously quantified in tormentil rhizomes, with the relative standard errors of prediction in the 2.0-4.9% range for both calibration and validation sets. These error ranges were found to be slightly higher for infrared techniques and amounted to 2.7-6.5%.
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