4.7 Article

BODIPY-based fluorescent sensor for imaging of endogenous formaldehyde in living cells

Journal

TALANTA
Volume 189, Issue -, Pages 274-280

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.07.001

Keywords

BODIPY; Formaldehyde; Fluorescent sensor; Endogenous and exogenous; Gaseous detection

Funding

  1. Ministry of Science and Technology of China [2014DFA31890]
  2. Natural Science Foundation of China [21771092]
  3. State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, China

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A new highly selective fluorescent chemosensor for formaldehyde (FA) has been synthesized based on boron dipyrromethene as fluorophore and o-phenylenediamine (OPDA) as reaction group. When FA is added, the fluorescence emission band of the chemosensor red shift (from 525 nm to 548 nm) accompanied by an increase in intensity with strong green fluorescence was observed. This chemosensor also exhibited the lowest detection limit (0.104 mu M) distinguished with other articles that have been reported. The application to cellular fluorescence imaging or test papers detection both indicated that the probe was highly responsive to the FA in endogenous cells and in the gaseous environment.

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