Article
Chemistry, Applied
Chuanyun Peng, Shaowen Zhang, Chunlai Wu, Yong Feng, Dan Zhao, Xiaoqing Wang, Zhengyu Bai
Summary: A facile in-tube solid phase microextraction procedure was developed to enrich ractopamine before HPLC-UV analysis, using an amide groups modified polysaccharide-silica hybrid monolith as an efficient sorbent. The method showed ideal selectivity for ractopamine, with enrichment factors ranging from 50.5 to 1.8 and recoveries from 85.2% to 108.1%.
Article
Biochemical Research Methods
Lubei Dong, Yihui Chen, Shubing Chen, Chunyan Hou, Shangyou Wang, Rongrong Xuan, Keyi Fang, Tingting Wang, Lihua Zhang, Yukui Zhang
Summary: A novel method involving in-tube solid-phase microextraction (SPME) using attapulgite nanoparticles-based hydrophobic monolithic column was successfully developed for the determination of three phosphodiesterase-5 (PDE-5) inhibitors in functional foods. The method showed good reproducibility and low limits of detection, making it a promising alternative for daily monitoring of PDE-5 inhibitors in functional foods.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Chemical
Jiangyi Wu, Youfang Huang, Xiaojia Huang
Summary: An efficient method using electroenhanced solid phase microextraction (EE-SPME) with porous monolith doped with carboxylic carbon nanotubes (M@CCNs) was developed to trap polar aromatic amines (AAs) in water samples. The introduction of M@CCNs and application of electric field during the extraction process significantly improved the trap performance, with extraction efficiencies increasing by 36-48% compared to traditional methods. The method was demonstrated to be practical and reliable for quantifying AAs in water samples, with recoveries ranging from 83.1% to 118%.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Engineering, Environmental
Xiaochong Song, Jiangyi Wu, Jinling Pang, Yuanfei Wu, Xiaojia Huang
Summary: A novel sorbent based on task specific monolith doped with Fe3O4 was developed for the in-tube solid phase microextraction (IT-SPME) of mercury species, with the addition of a magnetic field enhancement. This method proved effective in quantifying mercury at ultra-trace levels in various water samples, with high precision and reliability.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemical Research Methods
Xiaochong Song, Xin Meng, Minshi Chen, Lei Wang, Xiaojing Li, Xiaojia Huang
Summary: A new procedure using magnetism-enhanced in-tube solid phase microextraction (ME/IT-SPME) coupled with HPLC with diode array detector (DAD) has been developed for the online measurement of tetraethyllead (TEL) in aqueous samples. The ME/IT-SPME column, made of porous monolith mixed with Fe3O4 nanoparticles, was designed to incorporate a magnetic coil for the application of variable magnetic fields. The results showed a 52% improvement in extraction efficiency when a magnetic field was applied. The developed method demonstrated good precision, with a low limit of detection and high recovery rates.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Qianchun Zhang, Xiaolan Zhang, Bingnian Yang, Shan Liu, Ming Wen, Linchun Bao, Li Jiang
Summary: A novel method for the analysis of trace hydroxyl polycyclic aromatic hydrocarbons in biological samples was developed, which successfully detected several hydroxyl polycyclic aromatic hydrocarbons and achieved satisfactory recoveries in urine and whole blood samples. The use of a macroporous monolith proved to be an efficient approach for enriching trace hydroxyl polycyclic aromatic hydrocarbons in biological samples.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Chemistry, Analytical
Hexun Chen, Jiangyi Wu, Qingbiao Xiong, Xiaojing Li, Xiaojia Huang
Summary: In order to efficiently capture fluoroquinolones (FQs) from complex samples, a hybrid metal-organic framework (MOF)@monolith material (HMM) was developed and used as the extraction medium for multi-monolith fibers solid phase microextraction (MMF-SPME). The HMM/MMF-SPME exhibited satisfactory capture performance with high sensitivity and good precision. The developed technique can be a prominent method for capturing trace FQs in complex samples.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Jinling Pang, Hexun Chen, Xiaojia Huang
Summary: A new method for chromium speciation was proposed by combining magnetism-assisted in-tube solid phase microextraction with high-performance liquid chromatography. The method showed high extraction efficiency for Cr(III) and Cr(VI) complexes, with detection limits of 0.0059 ?g/L and 0.0020 ?g/L in water samples, and 0.47 ?g/kg and 0.057 ?g/kg in soil samples. The successful quantification of trace levels of Cr(III) and Cr(VI) in environmental samples demonstrated the reliability and practicality of the approach.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Hexun Chen, Xiaochong Song, Xiaojia Huang
Summary: A magnetism-assisted in-tube solid phase microextraction method was developed for capturing phenolic acids in fruit juices, with the application of magnetic fields enhancing extraction efficiency. This approach showed promising potential for highly sensitive quantification of trace phenolic acids in complex samples.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Chemistry, Applied
Jie Liu, Qian Liu, Liulin Wei, Xiaomei Chen, Zhiqiang Li, Yidong Xu, Xueyun Gao, Xiaoxiao Lu, Jingchan Zhao
Summary: This study proposed an on-line in-tube solid-phase microextraction coupled with high-performance liquid chromatography method for the determination of bisphenols in milk, using a polyhedral oligomeric silsesquioxane (POSS)-hybrid monolith. The synthetic monolith displayed satisfying extraction performance for target BPs, with low limits of detection and good spiked recoveries. This method provides an alternative for the analysis of BPs in complex samples.
Article
Chemistry, Analytical
Ruichen Yang, Jiangyi Wu, Guoqing Lu, Xiaojia Huang
Summary: The study developed a functional groups-rich monolithic adsorbent for efficient capture of carbamate and triazole pesticides. The combination of FRMA/MF-SPME with HPLC-DAD provided a sensitive and reliable method for trace analysis of pesticides in water samples, showing good efficiency, accuracy, and reproducibility.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Tiantian Pang, Xiaohui Chen, Kai Hu, Yongxia Cui, Wenjie Zhao, Huahui Zeng, Zhenqiang Zhang, Shusheng Zhang
Summary: A new Ti3C2Tx-coated fiber was synthesized and utilized for solid-phase microextraction of polychlorinated biphenyls. The method showed good linearity, high enrichment factors, low limits of detection, and excellent recoveries in river water, lake water, and tap water samples, indicating its promising potential as an alternative analysis method for polychlorinated biphenyls.
JOURNAL OF SEPARATION SCIENCE
(2021)
Article
Biochemical Research Methods
Jiangyi Wu, Hexun Chen, Xiaojia Huang
Summary: In this study, a novel solid-phase microextraction technique was developed using a composite material to efficiently extract trace PCAHs by applying electric fields. The method demonstrated wide linear ranges, low detection limits, and good precision for quantifying PCAHs, and the extraction mechanism was further elucidated. The approach was successfully applied in environmental monitoring of PCAHs with confirmed accuracy.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Biochemical Research Methods
Jiangyi Wu, Xiaojia Huang
Summary: A new strategy based on electric field-reinforced solid phase microextraction (ER-SPME) was proposed for the analysis of trace inorganic anions in aqueous and wine samples. Anion-exchange monolith (AEM) was synthesized on the surface of stainless steel wire and used as the adsorbent of ER-SPME. Electric fields were applied during the adsorption and desorption durations, resulting in accelerated extraction process and enhanced extraction efficiencies. The developed method, combining AEM/ER-SPME with ion chromatography and conductivity detector, exhibited high sensitivity, wide linear ranges, low limits of detection, and satisfactory precision for the quantification of trace investigated anions in various samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Analytical
Xiaochong Song, Xiaojing Li, Jingjuan Wang, Xiaojia Huang
Summary: In this study, a new strategy was introduced to improve the extraction efficiency and recognition performance of MIPs-based IT-SPME by implementing magnetic fields during the extraction stage. The application of magnetic field improved the specific extraction performance towards the template, resulting in increased extraction efficiency and imprinting factor. The magnetism-assisted IT-SPME technique based on MIP showed high sensitivity, good precision, and satisfactory recoveries when coupled with HPLC/DAD for quantifying trace analytes.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).