Journal
TALANTA
Volume 182, Issue -, Pages 279-284Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.01.076
Keywords
Laser induced fluorescence; Compact; Low-cost; Single cell
Categories
Funding
- Young Elite Scientists Sponsorship Program by China Association for Science and Technology [2016QNRC001]
- DICP [DICP ZZBS201609]
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A compact and low-cost laser induced fluorescence (LIF) detector based on confocal structure for capillary flow systems was developed and applied for analysis of Her2 protein on single Hela cells. A low-power and low-cost 450 nm laser diode (LD) instead of a high quality laser was used as excitation light source. A compact optical design together with shortened optical path length improved the optical efficiency and detection sensitivity. A superior silicon based photodetector assembly was used for fluorescence detection instead of a photomultiplier (PMT). The limit of detection (LOD) for fluorescein sodium was 3 x 10(-12) M or 165 fluorescein molecules in detection volume measured on a homemade capillary electroosmotic driven (EOD)-LIF system, which was similar to commercial LIFs. Compared to commercial LIFs, the whole volume of our LIF was reduced to 1/2-1/3, and the cost was less than 1/3 of them.
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