Article
Chemistry, Analytical
Haojie Cai, Aikun Liu, Mingming Zhang, Jun Song, Pengju Zeng, Junle Qu, Jiaqing Guo, Hao Li
Summary: This study presents a simple strategy for preparing N-doped carbon dots (NCDs) using hydrothermal synthesis. These NCDs, as probes, exhibit high sensitivity and selectivity for detecting and visualizing HS-. Additionally, NCDs demonstrate good photostability, biocompatibility, and low cytotoxicity.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Hua He, Xiaoliang Chen, Zhenzhen Feng, Lihua Liu, Qian Wang, Simin Bi
Summary: The nucleolus is a central hub for coordinating cellular stress responses during cancer development and treatment. Accurate identification of nucleolar stress response is crucially desired for nucleolus-based diagnostics and therapeutics, which is technically challenging due to the need to address ultrastructural analysis. A protein-like CD has been reported with the integration of fluorescent blinking domains and RNA-binding motifs, offering enhanced super-resolution imaging of the nucleolar ultrastructure for extracting multidimensional information and accurate distinguishment of different cell types. This CD-depicted nucleolar ultrastructure serves as a sensitive hallmark to identify and discriminate subtle responses to various stressors and provide RNA-related information inaccessible by conventional immunofluorescence methods, potentially becoming a broadly useful probe for nucleolar stress studies in cell diagnostics and therapeutics.
Article
Chemistry, Physical
Jiahui Shi, Juan Ning, Guizhen Hu, Wenzheng Guo, Yanmei Zhou
Summary: In this study, a series of panchromatic carbon dots were synthesized by using hydroxyurea and o-phenylenediamine as precursors and varying the solvents. The pink carbon dots (p-CDs) with a quantum yield of 46% were chosen for cellular research. After modifying the p-CDs with mannose, they could selectively target liver cancer cells and achieve naked-eye discrimination.
APPLIED SURFACE SCIENCE
(2023)
Article
Oncology
Junting Tao, Hao Zou, Xiaokun Liao, Xinjian Lu, Jun Cao, Jiaqi Pan, Chaorong Li, Yingying Zheng
Summary: This study prepared green fluorescent carbon dots and modified them into folic acid functionalized carbon dots and hyaluronic acid functionalized carbon dots with targeted function to study their application in breast cancer cells imaging. The results showed that the functionalized carbon dots had good water solubility and cytocompatibility, as well as targeting properties, which could be used to accurately identify breast cancer cells and normal cells.
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
(2022)
Article
Polymer Science
Hassan Namazi, Malihe Pooresmaeil, Roya Salehi
Summary: In this study, blue-emissive carbon dots (CDs) supported glyco-copolymer brushes functionalized D-mannose were prepared for targeted co-anticancer drug delivery to breast cancer cells. The CDs-poly(NIPAM-co-MAH)-D-Man nanocomposite displayed controlled drug release behavior and good biocompatibility in vitro. The percentage of arrested cells in sub G1 phase for the treated cells with DOX@CUR@CDs-poly(NIPAM-co-MAH)-D-Man was 100%, indicating the high efficiency of the nanocomposite in cancer therapy.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Chemistry, Analytical
Hao Wu, Lan-Fang Pang, Na Wei, Xiao-Feng Guo, Hong Wang
Summary: The study presented N-doped CDs (MTCDs) with superb nucleus-targeted ability for selectively detecting ClO-. MTCDs exhibit high fluorescence quantum yields and excellent nuclear localization capabilities, making them a promising tool for cellular imaging.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Christian Mark Pelicano, Jiaxin Li, Maria Cabrero-Antonino, Ingrid F. Silva, Lu Peng, Nadezda V. Tarakina, Sergio Navalon, Hermenegildo Garcia, Markus Antonietti
Summary: We have successfully designed carbon/potassium poly(heptazine imide) heterostructures via a one-step salt-melt assisted condensation method for efficient photocatalysis. By hybridizing adenine-derived carbonaceous material (Ad-carbon) with KPHI, the photocatalytic activity for H-2 evolution and H2O2 production significantly improved. We found that Ad-carbon acts as both an electron acceptor and a photosensitizer, and the intimate heterojunction between Ad-carbon and KPHI promotes charge separation and extends carrier lifetime. Electrocatalysis experiments confirmed that coupling Ad-carbon with KPHI enhances surface reaction kinetics for H-2 evolution and O-2 reduction reactions. The visual evidence of superior charge transport in the hybrid photocatalyst is demonstrated by the uniform distribution of smaller Pt nanoparticles on the carbon regions, which contributes to the increased catalytic activity.
JOURNAL OF MATERIALS CHEMISTRY A
(2023)
Article
Chemistry, Analytical
Shaobing Zhang, Haidong Yan, Hongni Li, Tiantian Xu, Hui Li, Chengkun Wang, Zheng Yang, Xiaodan Jia, Xiangrong Liu
Summary: Nitrogen-doped carbon dots (NCDs) were synthesized using coal washing wastewater as the carbon source, tryptophan for nitrogen doping, and surface functionalization with polyethylene glycol. The NCDs exhibited excellent fluorescent properties and could be used as fluorescent ink for message encryption, as well as fluorescent sensors for Hg2+ and GSH detection in various forms. The NCDs also showed low cytotoxicity and good biocompatibility, making them suitable for imaging Hg2+ and GSH in living cells. This study demonstrates the potential applications of NCDs in anti-counterfeiting, message encryption, and environmental/biological sensing.
Article
Chemistry, Multidisciplinary
Ke Cheng, Henggang Wang, Shan Sun, Mingyu Wu, Hongzhe Shen, Ke Chen, Zhiyuan Zhang, Si Li, Hengwei Lin
Summary: This study develops a strategy for specific chemiluminescence (CL) imaging and enhanced photodynamic therapy (PDT) of bacterial infections using hemin-modified carbon dots (H-CDs). The H-CDs exhibit favorable water solubility, augmented photodynamic activity, and unique peroxidase-mimicking capacity. The H-CDs not only enable infection identification via CL imaging but also enhance bactericidal effects through oxygen self-supply and relief of hypoxia. This work provides an effective antibacterial platform for selective imaging-guided treatment of bacterial infections.
Correction
Chemistry, Multidisciplinary
E. Shuang, Quan-Xing Mao, Jian-Hua Wang, Xu-Wei Chen
Summary: This study corrected the research on carbon dots with tunable dual emissions and explored their application in specific imaging of endoplasmic reticulum polarity.
Article
Chemistry, Multidisciplinary
Ronghui Li, Nana Shi, Kaiyue Sun, Mei Fang, Ziyan Zhang, Lina Geng, Jianjun Zhang
Summary: Nitrogen-doped carbon dots (N-CDs) were synthesized via a one-step hydrothermal method using citric acid and hexamethylenete-tramine. The N-CDs exhibited both down- and up-conversion fluorescence properties with an average particle size of 4.28 nm. These N-CDs were used as a drug carrier for doxorubicin (DOX) with a drug-loading rate of 35.88%. The N-CDs-DOX system displayed sustained-release and pH-targeted properties.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yujia Zheng, Qianqian Li, Jing Wu, Ziyi Luo, Wenyi Zhou, Anguo Li, Yanling Chen, Tuerxunayi Rouzi, Tian Tian, Hui Zhou, Xiaodong Zeng, Yang Li, Xiaoding Cheng, Yongchang Wei, Zixin Deng, Fuling Zhou, Xuechuan Hong
Summary: Novel NIR-II small-molecule probe H4-PEG-Glu showed effective imaging and treatment targeting mitochondria in acute myeloid leukemia cells, inducing cell apoptosis and exhibiting moderate cytotoxicity without laser irradiation. Enhanced chemo- and photothermal therapy effects were observed with laser irradiation, presenting high synergistic effects in vivo and specific tumor imaging in AML patient-derived PDX mouse models.
Article
Materials Science, Multidisciplinary
Xiaoxiao Gao, Yan Zhang, Zheng Fu, Fengling Cui
Summary: Carbon dots synthesized through hydrothermal method have stable properties, small particle size and good water solubility, suitable for cell imaging and lysosome targeting. They exhibit different colors under different UV lights, which can be used for information encryption. Additionally, the fluorescence of CDs varies at different pH levels, making them useful for pH measurement in water.
Article
Chemistry, Analytical
Dan Chang, Zhonghua Zhao, Lihong Shi, Wenliang Liu, Yongxing Yang
Summary: A colorimetric and fluorescent dual mode sensor based on lysosome-targeted CDs has been successfully implemented to identify Fe3+ fluctuations in vitro and in vivo, providing rapid and reliable recognition with exceptional stability, biocompatibility, and low cytotoxicity. The CDs can target lysosomes in cells and respond to Fe3+ fluctuations, showing promising potential for disease detection in physiology and pathology.
Article
Chemistry, Analytical
Lan-Fang Pang, Yu-Cheng Sun, Xiao-Feng Guo, Hong Wang
Summary: In this study, carbon dots with near-infrared fluorescence were prepared using a hydrothermal method and successfully used for quantitative determination of H2S and imaging of endogenous H2S efflux from cell membrane. The NIR-CDs showed specific location on cell membrane and offered a new strategy for the design of targeted fluorescent probes in different functional regions of the cell membrane in the future.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).