Journal
TALANTA
Volume 186, Issue -, Pages 406-412Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.04.080
Keywords
MiRNA imaging; Activatable fluorescence probes; Cancer cells; Cancer diagnosis
Categories
Funding
- NSFC [21335004, 21675088]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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This work reports an approach for imaging the localization, distribution, and level of miRNA in different or-ganelles based on an activated fluorescence signal triggered by an alteration of the specific binding-induced conformation of the designed activatable probe. We selected miR-150 as an miRNA example to image its localization, distribution, and level in human cervical cancer cells (HeLa cells). The results indicate that miR-150 is localized and distributed in different subcellular organelles (mainly in mitochondria and lysosomes) and that its levels (actually its concentrations) in lysosomes are higher than those in mitochondria in both HeLa and MCF-7 cells. Moreover, the level of miRNA in cells is displayed in a height-dependent (in z-direction) manner. This approach can also be used to image the localization and distribution of various miRNAs (such as miR-150 and miR-214) in different organelles in cancer cells simultaneously. The probes exhibit high resistance to cellular endo- and exonucleases, with high specificity; the capability of avoiding false signals, with a high signal-to background ratio; and a good ability to operate in complicated environments. The developed approach may provide a useful tool for studying the localization and distribution and evaluating the level of multiple tumor related miRNAs in cells.
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