Journal
SYNAPSE
Volume 72, Issue 12, Pages -Publisher
WILEY
DOI: 10.1002/syn.22060
Keywords
[C-11](R)PK11195; adeno-associated viral vectors; alpha-synuclein; animal model; inflammation; minipig; Parkinson's disease
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Funding
- Lundbeck Foundation [2013-16034]
- Bjarne Saxhof Fund
- European Union's Seventh Framework Programme grant (FP7) [603646]
- EU FP7 (2007-2013) [HEALTH-F2-2011-278850]
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Parkinson's disease is characterized by a progressive loss of substantia nigra (SN) dopaminergic neurons and the formation of Lewy bodies containing accumulated alpha-synuclein (alpha-syn). The pathology of Parkinson's disease is associated with neuroinflammatory microglial activation, which may contribute to the ongoing neurodegeneration. This study investigates the in vivo microglial and dopaminergic response to overexpression of alpha-syn. We used positron emission tomography (PET) and the 18 kDa translocator protein radioligand, [C-11](R)PK11195, to image brain microglial activation and (+)-alpha-[C-11]dihydrotetrabenazine ([C-11]DTBZ), to measure vesicular monoamine transporter 2 (VMAT2) availability in Gottingen minipigs following injection with recombinant adeno-associated virus (rAAV) vectors expressing either mutant A53T alpha-syn or green fluorescent protein (GFP) into the SN (4 rAAV-alpha-syn, 4 rAAV-GFP, 5 non-injected control minipigs). We performed motor symptom assessment and immunohistochemical examination of tyrosine hydroxylase (TH) and transgene expression. Expression of GFP and alpha-syn was observed at the SN injection site and in the striatum. We observed no motor symptoms or changes in striatal [C-11]DTBZ binding potential in vivo or striatal or SN TH staining in vitro between the groups. The mean [C-11](R)PK11195 total volume of distribution was significantly higher in the basal ganglia and cortical areas of the alpha-syn group than the control animals. We conclude that mutant alpha-syn expression in the SN resulted in microglial activation in multiple sub- and cortical regions, while it did not affect TH stains or VMAT2 availability. Our data suggest that microglial activation constitutes an early response to accumulation of alpha-syn in the absence of dopamine neuron degeneration.
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