Article
Multidisciplinary Sciences
Joshua Kim, Krista Chun, Jenna McGowan, Youjie Zhang, Piotr J. Czernik, Blair Mell, Bina Joe, Saurabh Chattopadhyay, Joseph Holoshitz, Ritu Chakravarti
Summary: Inflammatory arthritis (IA) is a common disease affecting millions worldwide. 14-3-3 zeta protein plays a role in immune suppression and extracellular remodeling, leading to previously unrecognized IA-suppressive function. Immunization with recombinant 14-3-3 zeta protein can suppress IL-1 beta levels and inhibit the progression of IA.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Pia Niemann, Miriam Schiffer, Daniela Malan, Sabine Gruenberg, Wilhelm Roell, Caroline Geisen, Bernd K. Fleischmann
Summary: This study reports the establishment of a Cre-inducible Cx43 overexpression system in murine embryonic stem cells, which enables the investigation of the impact of Cx43 overexpression in somatic cells. The analysis reveals the formation of functional gap junctions and the differentiation of Cx43-overexpressing cells into viable cardiomyocytes.
Article
Cell Biology
Peipei Wu, Bin Zhang, Xinye Han, Yaoxiang Sun, Zixuan Sun, Linli Li, Xinru Zhou, Qian Jin, Peiwen Fu, Wenrong Xu, Hui Qian
Summary: Research has found that exosomes derived from human umbilical cord mesenchymal stem cells (hucMSC-ex) have antioxidant and anti-inflammatory effects against UV radiation-induced skin photodamage by activating SIRT1 to reduce oxidative stress and promote autophagy, thereby alleviating cytotoxic damage. The delivery of 14-3-3 zeta protein by hucMSC-ex plays a cytoprotective role through modulation of a SIRT1-dependent antioxidant pathway. Overall, hucMSC-ex may serve as a potential agent for preventing or treating UV radiation-induced skin photodamage and aging.
Article
Neurosciences
Cuixian Li, Shen Huang, Jin Peng, Tianguo Hong, Chun Zhou, Jie Tang
Summary: In this study, a new cellular protein, 14-3-3 zeta, was found to interact with the beta subunit of GABA(A)Rs and BIG1, and co-localize with them in hippocampal neurons. Overexpression of 14-3-3 zeta increased the surface expression of BIG1 and its binding with GABA(A)R. Depletion of 14-3-3 zeta or BIG1 significantly decreased GABA(A)R expression at the cell surface and inhibited GABA-gated influx of chloride ions.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Pathology
Dan Wan, Yutao Zhang, Qin Yu, Feng Li, Junju Zhuo
Summary: The study revealed that the co-overexpression of 14-3-3 and HER2 in breast cancer could enhance the occurrence, development, and lymph node metastasis of the cancer, suggesting it may impact the treatment decisions and prognosis of breast cancer.
PATHOLOGY RESEARCH AND PRACTICE
(2021)
Article
Cell Biology
Lingyun Wei, Nan Hu, Mingxiang Ye, Zhilong Xi, Zhen Wang, Lei Xiong, Nan Yang, Yi Shen
Summary: This study found that high expression of 14-3-3ζ in NSCLC tissues is associated with tumor recurrence. High expression of 14-3-3ζ in NSCLC cells was found to enhance motility and invasive capacity, and induce epithelial-mesenchymal transition. Additionally, high expression of 14-3-3ζ was also found to promote proliferation and resistance to chemotherapy-induced apoptosis in NSCLC cells.
Article
Neurosciences
Yue Chen, Jiagui Huang, Jie Liu, Huimin Zhu, Xuemei Li, Jun Wen, Mingfen Tian, Jiangxia Ren, Li Zhou, Qin Yang
Summary: This study investigates the role of Sirt1 in the formation of fibrotic scar after cerebral ischemic stroke. The results show that Sirt1 can reduce the formation of fibrotic scar and promote neurological function recovery, possibly through regulating the deacetylation of 14-3-3 zeta.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Fisheries
Rui Xue, Dinglong Yang, Yijing Han, Qinyou Deng, Xin Wang, Xiangquan Liu, Jianmin Zhao
Summary: The 14-3-3 zeta and 14-3-3 epsilon proteins play important roles in innate immunity against V. harveyi infection in mollusks, with their differential expression leading to different immune responses.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yue Han, Haiqiong Ye, Ping Li, Yifan Zeng, Jing Yang, Meng Gao, Zhengding Su, Yongqi Huang
Summary: Tau, a major microtubule-associated protein, can form liquid droplets. 14-3-3 zeta protein can be recruited into tau droplets and regulate their formation through electrostatic and hydrophobic interactions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Multidisciplinary Sciences
Jacinth Rajendra, Atanu Ghorai, Shilpee Dutt
Summary: The study identified significant up-regulation of 14-3-3 zeta in residual resistant GBM cells, and knocking down this protein sensitized GBM cells to radiation therapy. Additionally, reduction of 14-3-3 zeta resulted in increased mitochondrial content in residual cells.
Article
Biochemistry & Molecular Biology
Sunayana Dagar, Kumari Pushpa, Diksha Pathak, Sarbani Samaddar, Anjana Saxena, Sourav Banerjee, Sivaram V. S. Mylavarapu
Summary: This study demonstrates a novel mRNA-guided mechanism of TNT formation through the maintenance of cellular 14-3-3 zeta mRNA levels by the RBP nucleolin.
Article
Oncology
Mingda Zhao, Yibing Zhang, Longfei Li, Xiaobin Liu, Wenping Zhou, Chunhui Wang, Yufu Tang
Summary: Our study found that KHDRBS3 is highly expressed in human HCC tissues and predicts poor prognosis. Knockdown of KHDRBS3 inhibits tumor growth, reduces glycolysis, enhances sensitivity to doxorubicin, and induces apoptosis in HCC cells. On the contrary, overexpression of KHDRBS3 promotes malignant behavior. KHDRBS3 interacts with YWHAZ, and the promotion of proliferation and glycolysis and the inhibition of apoptosis caused by KHDRBS3 overexpression can be reversed by silencing 14-3-3ζ.
CANCER CELL INTERNATIONAL
(2023)
Article
Oncology
Tahereh Kashkoulinejad-Kouhi, Shahrokh Safarian, Blanca Arnaiz, Laura Saa
Summary: Cisplatin resistance in MCF-7 cells is enhanced by downregulating BiP and 14-3-3 zeta proteins to promote apoptosis, activating UPR to counteract apoptotic pathways and increase sensitivity to cisplatin.
Article
Chemistry, Multidisciplinary
Phillip Zhu, Stanislau Stanisheuski, Rachel Franklin, Amber Vogel, Cat Hoang Vesely, Patrick Reardon, Nikolai N. Sluchanko, Joseph S. Beckman, P. Andrew Karplus, Ryan A. Mehl, Richard B. Cooley
Summary: 14-3-3 proteins dimerize and bind phosphorylated clients to regulate their function. By introducing stable mimics of phosphorylated amino acids, researchers can study 14-3-3 function effectively. In this study, the authors improved a previous genetic code expansion system to incorporate nonhydrolyzable phosphoserine into proteins. They successfully produced biologically relevant proteins with the modified amino acid and investigated the effects of phosphorylation on protein interactions and function.
ACS CENTRAL SCIENCE
(2023)
Article
Cell Biology
Yuan Huang, Shi Li, Qinfeng Liu, Zhijie Wang, Shunyao Li, Lei Liu, Weiwei Zhao, Kai Wang, Rui Zhang, Longfei Wang, Ming Wang, Declan William Ali, Marek Michalak, Xing-Zhen Chen, Cefan Zhou, Jingfeng Tang
Summary: This study reveals that lymphocyte-specific protein tyrosine kinase (LCK) directly interacts with transient receptor potential melastatin 8 (TRPM8) and enhances its phosphorylation at Y1022. LCK positively regulates TRPM8 channel function by increasing TRPM8 multimerization. Additionally, the interaction between TRPM8 and 14-3-3 zeta positively modulates channel multimerization. The phosphorylation of TRPM8 at Y1022 plays a crucial role in the proliferation, migration, and tumorigenesis of pancreatic cancer cells.
CELL DEATH & DISEASE
(2022)
Article
Genetics & Heredity
Qianqian Sha, Wei Zheng, Xie Feng, Ruiying Yuan, Huiling Hu, Fei Gong, Liang Hu, Ge Lin, Xianghong Ou
Summary: Mutations in TUBB8 gene may lead to the formation of MPN, resulting in infertility cases. These new findings may be helpful for genetic counseling of infertile patients with MPN.
Article
Genetics & Heredity
Dehua Cheng, Liang Hu, Fei Gong, Shimin Yuan, Keli Luo, Xianhong Wu, Pingyuan Xie, Changfu Lu, Guangxiu Lu, Yue-Qiu Tan, Ge Lin
Summary: This study evaluated the clinical outcomes of ABCR carrier couples undergoing the MicroSeq-PGT technique, showing its powerful ability to distinguish between carrier and non-carrier balanced embryos. The study involved 762 ABCR carrier couples and demonstrated successful transfer of both carrier and normal embryos, resulting in healthy babies with no developmental issues.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Genetics & Heredity
Jing Dai, Tianlei Zhang, Jing Guo, Qinwei Zhou, Yifan Gu, Jue Zhang, Liang Hu, Yurong Zong, Juan Song, Shuoping Zhang, Can Dai, Fei Gong, Guangxiu Lu, Wei Zheng, Ge Lin
Summary: Genetic variations in the ACTL9 gene in male individuals have been found to cause total fertilization failure (TFF). These variations disrupt the perinuclear theca structure and affect the localization of PLCz in sperm, leading to abnormal calcium oscillations in oocytes and subsequent TFF. Overcoming TFF can be achieved through assisted oocyte activation with calcium ionophore exposure.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Physiology
Mengge Li, Yaxing Ji, Dongmei Wang, Yanliang Zhang, Huan Zhang, Yi Tang, Ge Lin, Liang Hu
Summary: This study investigated the efficiency of Raman spectroscopy in detecting sperm chromosome balance state by analyzing DNA content differences. The results showed that different regions of sperm have different chemical components and DNA content, allowing for preliminary classification based on DNA content. X sperm were found to have significantly higher DNA backbone peaks and regional peaks compared to Y sperm, confirming the different sperm types.
FRONTIERS IN PHYSIOLOGY
(2022)
Correction
Oncology
Chunyu Zhang, Li Fu, Jianhua Fu, Liang Hu, Hong Yang, Tie-Hua Rong, Yan Li, Haibo Liu, Song-Bin Fu, Yi-Xin Zeng, Xin-Yuan Guan
CLINICAL CANCER RESEARCH
(2022)
Article
Multidisciplinary Sciences
Jian Li, Jinhua Shen, Xiaoli Zhang, Yangqin Peng, Qin Zhang, Liang Hu, Christoph Reichetzeder, Suimin Zeng, Jing Li, Mei Tian, Fei Gong, Ge Lin, Berthold Hocher
Summary: In vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) is associated with an increased risk of preterm and early preterm birth. Older or younger maternal age, multiple pregnancy, placenta previa, and embryo reduction are independent risk factors for preterm birth and early preterm birth. Gestational hypertension, elevated triglycerides, and shorter activated partial thromboplastin time are only associated with preterm birth. Only embryo reduction represents an IVF/ICSI-specific risk factor.
SCIENTIFIC REPORTS
(2022)
Article
Genetics & Heredity
Pingyuan Xie, Liang Hu, Yangqin Peng, Yue-qiu Tan, Keli Luo, Fei Gong, Guangxiu Lu, Ge Lin
Summary: This study analyzed factors influencing meiotic segregation patterns in blastocysts for reciprocal translocation carriers. The results showed that an increase in TAR1 was associated with an increase in alternate segregation, while female sex and involvement of an acrocentric chromosome reduced the proportion of alternate segregation. These findings are important for providing more appropriate genetic counseling to couples regarding their chances of producing normal/balanced blastocysts.
FRONTIERS IN GENETICS
(2022)
Article
Medicine, General & Internal
Jin Huang, Li Rong, Lin Zeng, Liang Hu, Juanzi Shi, Liyi Cai, Bing Yao, Xiu-Xia Wang, Yanwen Xu, Yuanqing Yao, Yan Wang, Junzhao Zhao, Yichun Guan, Weiping Qian, Guimin Hao, Sijia Lu, Ping Liu, Jie Qiao
Summary: This study aims to evaluate the non-invasive genetic testing method for embryonic aneuploidy and determine its clinical effectiveness in evaluating embryos.
Article
Genetics & Heredity
Pingyuan Xie, Ping Liu, Shuoping Zhang, Dehua Cheng, Dayang Chen, Yue-Qiu Tan, Liang Hu, Yong Qiu, Shuang Zhou, Qi Ou-Yang, Keli Luo, Guangxiu Lu, Shaohong Zhang, Fei Gong, Ge Lin
Summary: This study investigated the spectrum and characteristics of segmental aneuploidies (SAs) of 10 Mb length in human preimplantation blastocysts. The results showed that 1 to 10 Mb SAs are common in blastocysts, and some of them have potential pathogenicity. Inheritance pattern analysis and clinical interpretation contribute to determining the pathogenic or likely pathogenic SAs.
GENETICS IN MEDICINE
(2022)
Article
Obstetrics & Gynecology
Pingyuan Xie, Xiao Hu, Lingyin Kong, Yan Mao, Dehua Cheng, Kai Kang, Jing Dai, Dingding Zhao, Yi Zhang, Naru Lu, Zhenxing Wan, Renqian Du, Bo Xiong, Jun Zhang, Yueqiu Tan, Guangxiu Lu, Fei Gong, Ge Lin, Bo Liang, Juan Du, Liang Hu
Summary: A multifunctional haplotyping-based preimplantation genetic testing platform was provided for detecting different genetic conditions.
HUMAN REPRODUCTION
(2022)
Article
Genetics & Heredity
Keya Tong, Wenbin He, Yao He, Xiurong Li, Liang Hu, Hao Hu, Guangxiu Lu, Ge Lin, Chang Dong, Victor Wei Zhang, Juan Du, Dongyun Liu
Summary: Medical exome sequencing was used to identify carrier spectrum in the Chinese population, showing that 94.5% of individuals carried at least one disease-causing variant. This study highlights the importance of Expanded Carrier Screening in identifying at-risk couples.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Tongyuan Dang, Pingyuan Xie, Zhiqi Zhang, Liang Hu, Yi Tang, Yueqiu Tan, Keli Luo, Fei Gong, Guangxiu Lu, Ge Lin
Summary: This study analyzed the factors influencing segregation and ploidy results in Robertsonian carriers and found that the segregation patterns were influenced by the carrier's sex and independent of their age. Additionally, advanced maternal age decreased the likelihood of obtaining normal/balanced embryos, and Robertsonian translocation chromosomes increased the possibility of chromosome mosaic during blastocyst mitosis.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Genetics & Heredity
Shuoping Zhang, Pingyuan Xie, Fang Lan, Yaxin Yao, Shujuan Ma, Liang Hu, Yueqiu Tan, Bo Jiang, Anqi Wan, Dunmei Zhao, Fei Gong, Sijia Lu, Ge Lin
Summary: This study aimed to investigate the feasibility of applying conventional in vitro fertilization (cIVF) for couples undergoing preimplantation genetic testing for aneuploidies (PGT-A) with non-male factor infertility. The results showed that under the Picoplex and ChromInst protocols, sperm DNA failed to amplify, but could be amplified using multiple displacement amplification (MDA). The study concluded that applying cIVF to couples with non-male factor infertility undergoing PGT-A is feasible.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Article
Andrology
Yuze Pi, Zhuo Huang, Xilin Xu, Huan Zhang, Miao Jin, Shuoping Zhang, Ge Lin, Liang Hu
Summary: This study found that patients' sperm mitochondrial DNA was more likely to carry pathogenic variants or variants of unknown significance and had higher heteroplasmy levels. Some patients lacked unique variants in their sperm but had exceptionally high mtDNA copy numbers. The heteroplasmy level of common mtDNA variants decreased, while a large number of novel mtDNA variants with low-level heteroplasmy emerged in sperm.
Article
Cell & Tissue Engineering
XiangWang Xu, JunRu Chen, Hao Zhao, YuZe Pi, Ge Lin, Liang Hu
Summary: This study used single-cell RNA sequencing to investigate the transcriptional landscape of a high-efficiency hESC-EC induction system. The FLI1-PKC induction system can differentiate hESCs into mature endothelial cells with all requisite functions, possibly due to the elevated number of activated transcription factors.
STEM CELL REVIEWS AND REPORTS
(2023)