Review
Cell Biology
Tae-Kyung Hong, Jae-Hoon Song, So-Been Lee, Jeong-Tae Do
Summary: Assisted reproductive technologies (ARTs) have limitations in rectifying germ cell aplasia such as non-obstructive azoospermia (NOA) and oocyte maturation failure syndrome. In vitro gametogenesis shows promise in overcoming infertility, with research focusing on deriving male and female germ cells from pluripotent stem cells (PSCs). This technique not only helps understand the biological mechanism of gamete development but also offers insights into its application in infertility treatments.
Article
Cell & Tissue Engineering
Xia Gao, Xin Liao, Jing Zhang, Jinai Lin, Mei Tan
Summary: A stem cell line GWCMCi001-A was established from PBMCs of a 4-year-old male patient with IgAN using episomal plasmids, serving as an ideal in vitro model to study the pathogenesis of IgAN.
STEM CELL RESEARCH
(2021)
Article
Cell Biology
Shuo Cao, Xinyue Gao, Fangyuan Liu, Yanglin Chen, Qin Na, Qiaoqiao Meng, Peng Shao, Chen Chen, Yongli Song, Baojiang Wu, Xihe Li, Siqin Bao
Summary: This study derived iPSCs from fibroblasts of a patient with acute myelitis and compared them to iPSCs from normal individuals. The patient-derived iPSCs showed attenuated potential for ectoderm differentiation, which was found to be related to the Wnt signaling pathway. This study provides a novel approach to investigating the pathogenesis of acute myelitis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell & Tissue Engineering
Yuqing Zhu, Li Zhang, Gang Wang, Jing Zhao, Xiaolin Hou, Hangdi Wu, Yuming Xu, Jianhua Mao, Zhihong Liu, Jin Zhang
Summary: In this study, PBMCs were collected from a patient with Fabry disease caused by a novel GLA gene mutation. An iPSC line was derived using an episomal reprogramming method, confirming the expected mutation and pluripotency status through various validation methods.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Ahmed K. Elsayed, Salam Salloum-Asfar, Sara A. Abdulla
Summary: A cell line, QBRIi013-A, derived from a 6-year-old female with ASD and intellectual disability, has been successfully generated using a non-integrating Sendai virus. This iPSC line exhibits pluripotency and differentiation potential, serving as a valuable cell model for understanding the molecular mechanisms underlying ASD pathogenesis.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Hui Li, Bei Zhang, Zhiqiang Shi, Hui Wu, Shuya Mei, Tianyi Ai, An Shi, Lihui Wang, Mingli Zhu, Jiajia Tang
Summary: In this study, an iPSC line was derived from a 69-year-old female patient with sporadic AD who carried APOE3/4 allele. This iPSC line will be valuable for investigating the underlying mechanisms of AD and for drug screening purposes.
STEM CELL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Zhuoya Wang, Huifang Sun, Qi Zhang, Jing Yang, Yutao Liu, Han Liu, Changhe Shi, Yanlin Wang, Yuming Xu
Summary: Through successful iPSC construction and validation from a female with an APP gene mutation, this study provides new prospects for Alzheimer's disease treatment and research.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Wei Dong, Dandan Shi, Lihua Zhang, Mingwei Huang, Qiang Luo
Summary: In this study, an iPSC line was successfully established from PBMCs of a five-year-old female patient with EIG15, showing stable expression of pluripotent genes, normal karyotype, and full potential for differentiation into three germ layers.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Long Wang, Beibei Gao, Xuming Mo, Xiaoling Guo, Jinyu Huang
Summary: By reprogramming human urine cells from an X-ALD patient, a stable iPSC line was established with pluripotent marker expression, differentiation potential, and normal karyotype.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Hong Wu, Yan Cheng, Zhenshan Lv, Lidi Liu, Weiquan Gong, Shaokun Zhang
Summary: Peripheral blood from a 48-year old healthy male was used to extract induced pluripotent stem cells (iPSC) reprogrammed with Sendai virus. The iPSC line exhibited pluripotency, normal karyotype, and the ability to differentiate into the 3 germ layers, making it a valuable tool for studying inherited diseases with a healthy control reference.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Morad Kamand, Sheena Louise Forsberg, Mads Thomassen, Mirolyuba Ilieva, Morten Meyer, Asa Fex Svenningsen, Tanja Maria Michel
Summary: Autism, characterized by communication challenges, social behavior issues, and repetitive conduct, has been studied using stem cell technologies to model underlying disease mechanisms. Researchers successfully generated a new iPS cell line from a patient diagnosed with atypical autism, which validated pluripotency marker expression, differentiation into three germ layers, and the absence of chromosomal abnormalities.
STEM CELL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Lin Zhang, Qiao Ren, Xiaohui Liu, Gang Liu, Shulin Meng, Kang Xiahou, Yongxiang Zhang, Ning Jiang, Wenxia Zhou
Summary: In this study, human induced pluripotent stem cells (iPSCs) were successfully generated from peripheral blood mononuclear cells (PBMCs) of an 87-year-old female patient with sporadic Alzheimer's disease (sAD) and APOE3 genotype. The pluripotency of the iPSC line was confirmed through embryoid body formation and flow cytometry analyses. The iPSC line exhibited a normal karyotype and could serve as a valuable tool for studying sAD pathogenesis.
STEM CELL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Eleanor Luce, Clara Steichen, Soumeya Abed, Anne Weber, Philippe Leboulch, Leila Maouche-Chretien, Anne Dubart-Kupperschmitt
Summary: The study demonstrates that siPSCs can differentiate into liver cells and self-organize into 3D structures, facilitating research on liver development and the development of co-culture systems.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Engineering, Biomedical
Camilla Luni, Onelia Gagliano, Nicola Elvassore
Summary: An integrative approach combining microfluidic design and stem cell biology allows for the capture of spatial-temporal environmental changes related to epigenetic remodeling and morphogenetic processes, contributing to significant advances in stem cell engineering.
ANNUAL REVIEW OF BIOMEDICAL ENGINEERING
(2022)
Article
Cell & Tissue Engineering
Ruiyun Guo, Weihong Mu, Xin Liu, Jing Zhang, Boxin Liu, Xiaofeng Du, Jingjing He, Jun Ma, Huixian Cui
Summary: A human induced pluripotent stem cell (iPSC) line was successfully derived from skin cells of a 48-year old male donor, which was integration-free, had a normal karyotype, expressed pluripotency markers, and showed potential for trilineage differentiation in vitro. This iPSC model will be valuable for studying physiological processes, drug validation, and serving as a control in pathological mechanistic studies.
STEM CELL RESEARCH
(2021)
