Article
Energy & Fuels
Zheyu Liu, Maojie Chai, Xin Chen, Seyed Hossein Hejazi, Yiqiang Li
Summary: Experimental investigation of flow regimes in a rectangular micmchannel using a water-oil system revealed four distinct flow regimes based on parameters such as viscosity ratios, velocity ratios, and capillary numbers. An exponential correlation was found between viscosity ratio and velocity ratio in transitioning flow regimes, with different effects on droplet size and slug length.
Article
Virology
Jialiang Du, Gang Wu, Chunbo Cui, Chuanfei Yu, Yongfei Cui, Luyun Guo, Yueyue Liu, Yan Liu, Wenbo Wang, Chunyu Liu, Zhihao Fu, Meng Li, Sha Guo, Xiaojuan Yu, Yalan Yang, Maoqin Duan, Gangling Xu, Lan Wang
Summary: In this study, HuNoV VLPs with different genogroups, genotypes, and sub-genotypes were biochemically fingerprinted using capillary isoelectric focusing with whole column imaging detection (cIEF-WCID). The results showed that these VLPs had different properties. This method holds great promise for evaluating the production consistency of HuNoV VLP vaccines.
Review
Chemistry, Analytical
Filip Dusa, Anna Kubesova, Jiri Salplachta, Dana Moravcova
Summary: Capillary isoelectric focusing (cIEF) is a powerful separation technique that requires the repeatable formation of a pH gradient and the use of internal standards to track its profile. This review provides an overview of available isoelectric point markers and their syntheses, as well as recent applications of cIEF in analyzing complex larger structures.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Filip Dusa, Dana Moravcova, Karel Slais
Summary: We present a set of novel low-molecular-mass compounds with ampholytic properties that can be used as markers of isoelectric point in different isoelectric focusing formats. These compounds have excellent focusing properties and stability, making them suitable for various isoelectric focusing methods.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Jialiang Du, Gang Wu, Quanyao Chen, Chuanfei Yu, Gangling Xu, Anhui Liu, Lan Wang
Summary: This study used capillary isoelectric focusing to investigate the charge heterogeneity of the SARS-CoV-2 RBD. The results showed that this method can be used for the identification, evaluation of batch-to-batch consistency, and stability study of the trimeric SARS-CoV-2 RBD as part of a quality control strategy during potential vaccine production.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Marta Napiorkowska, Luzius Pestalozzi, Sven Panke, Martin Held, Steven Schmitt
Summary: A high-throughput platform for optimizing microbial protein production is described, using droplet microfluidics and flow cytometry. The platform successfully cultures several industrially relevant bacterial and yeast strains and isolates strains with superior production titers by detecting peptides or proteins released from the cell via autolysis or secretion.
Article
Chemistry, Analytical
Ali M. Ibrahim, Jose I. Padovani, Roger T. Howe, Yasser H. Anis
Summary: This paper examines the parameters affecting droplet generation in a microfluidic system, develops a three-dimensional numerical model for simulation, and investigates the impact of different flow rates on droplet size.
Article
Chemistry, Analytical
Wensyang Hsu, Yu-Teng Shih, Meng-Shiue Lee, Hong-Yuan Huang, Wan-Ning Wu
Summary: This study investigated whether using a fixed number of beads in a bead-based digital microfluidic immunoassay could provide more stable results. The results showed that a fixed number of beads had smaller coefficient of variation compared to varying bead numbers, especially in the range of 1 pg/mL to 100 pg/mL. The use of a fixed number of beads significantly reduced the limit of detection.
Article
Engineering, Chemical
Wei Yu, Bo Li, Xiangdong Liu, Yongping Chen
Summary: A numerical research investigated the generation of triple emulsion droplets in a three-dimensional axisymmetric flow-focusing device. The study identified three flow regimes: dripping, jetting, and threading. The effects of operation conditions, such as flow rate and interfacial tension, on droplet generation were clarified, with viscosity also playing a significant role.
CHEMICAL ENGINEERING SCIENCE
(2021)
Review
Biochemical Research Methods
Gyula Vigh, Bohuslav Gas
Summary: In 1961, Svensson first described the principle of isoelectric focusing (IEF), which used a natural pH gradient formed by passing current through a sucrose density gradient-stabilized ampholyte mixture in a constant cross-section apparatus to separate ampholytic compounds. When polyacrylamide gel replaced the sucrose density gradient, the spatial and temporal stability of Svensson's pH gradient was lost, leading to a search for an explanation and improvement. It was later suggested that in the modern open-system IEF experiment, a two-stage process occurs, with a rapid first phase of linear pH gradient development followed by a slow second stage where isotachophoretic processes cause the pH gradient to decay as extreme pI CAs move into the electrode vessels.
Review
Chemistry, Analytical
Jiaqi Wu, Will McElroy, Janusz Pawliszyn, Christopher D. Heger
Summary: This article reviews the applications of icIEF technology in the pharmaceutical industry, highlighting the benefits of whole-column imaging detection for capillary isoelectric focusing. It also explores recent research using this technology to support advancements in the therapeutic landscape.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Gyula Vigh, Bohuslav Gas
Summary: In modern isoelectric focusing systems, the formation of stationary, steady-state pH gradients is prevented due to convective mixing prevention, varying current densities, and imbalanced electrophoretic and diffusive fluxes. Open-system IEF is described as a two-stage process involving rapid IEF and subsequent degradation of the pH gradient. The authors propose a single process, transient, bidirectional isotachophoresis (tbdITP) model for open-system IEF in their previous paper, and this paper demonstrates the operational features of the tbdITP model using Simul 6.
Article
Engineering, Chemical
Akepogu Venkateshwarlu, Ram Prakash Bharti
Summary: This study investigated the hydrodynamics of droplet generation in two-phase flow through a T-junction cross-flow microfluidic device by examining the influences of capillary number and flow rate ratio. It identified different flow regimes and presented correlations to guide the design of droplet microfluidics devices based on droplet size, detachment time, and generation frequency.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2021)
Article
Medical Laboratory Technology
Genhan Zha, Xuan Xiao, Youli Tian, Hengying Zhu, Ping Chen, Qiang Zhang, Changjie Yu, Honggen Li, Yuxing Wang, Chengxi Cao
Summary: A 30-mm pH 5.2-7.8 micro-column IEF array chip was developed for efficient screening of hemoglobinopathy, with higher resolution compared to traditional methods. Up to 24 samples can be simultaneously analyzed in a 12-minute run, and the method showed good sensitivity and specificity in comparison with LC and PCR.
CLINICA CHIMICA ACTA
(2023)
Article
Engineering, Electrical & Electronic
Yoshito Nozaki, Dong Hyun Yoon, Masahiro Furuya, Hiroyuki Fujita, Tetsushi Sekiguchi, Shuichi Shoji
Summary: A microfluidic device with a 3D structure was fabricated and tested for droplet generation, showing superior performance compared to conventional T-junction and cross-junction structures. The 3D device sheared the dispersed phase into droplets from multiple directions, leading to efficient droplet production across a wide range of fluid properties and flow conditions. Smaller droplets were produced using the 3D device, indicating its excellent droplet-generation performance.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Review
Chemistry, Analytical
Marco Morani, Thanh Duc Mai, Zuzana Krupova, Guillaume van Niel, Pierre Defrenaix, Myriam Taverna
Summary: Extracellular vesicles (EVs) are a family of cell-derived membrane vesicles present in almost all body fluids, playing important roles in various patho-physiological conditions. Knowledge about the features of individual EVs is still in its infancy, but electrokinetically driven methods provide new trends for isolation and characterization of EVs.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Ngoc Van Thanh Nguyen, Claire Smadja, Myriam Taverna, Sirine El Mousli, Emilie Secret, Jean-Michel Siaugue, Lac Thuy Huu Nguyen, Thanh Duc Mai
Summary: This study introduces a new approach for modulation and suppression of electroosmotic flow in capillary electrophoresis, achieving better sample preconcentration. By increasing buffer concentrations and utilizing large weakly charged molecules as background electrolyte constituents, the electroosmotic mobility can be modulated over a wide range without the drawbacks of high electric currents and Joule heating. The new method was successfully applied to sensitive determination of magnetic nanoparticles, offering significantly improved sensitivity compared to traditional methods.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Mihai Deleanu, Jean-Francois Hernandez, Luca Cipelletti, Jean-Philippe Biron, Emilie Rossi, Myriam Taverna, Herve Cottet, Joseph Chamieh
Summary: This study demonstrates the efficacy of modern Taylor dispersion analysis (TDA) in unraveling the speciation of beta-amyloid peptides in low-volume peptide samples with a short analysis time. TDA was able to provide a complete and quantitative picture of A beta speciation during the aggregation process, including the sizing of oligomers and protofibrils, the consumption of monomers, and the quantification of different early and late-formed aggregated species.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Etienne Laborie, Victor Le-Minh, Thanh Duc Mai, Mehdi Ammar, Myriam Taverna, Claire Smadja
Summary: This study presents a new analytical approach to assess antibody orientation and density on magnetic beads. Results showed that different functionalized beads have varying capture efficiency towards human TNF-alpha immunocapture, with Protein G beads providing the highest efficiency.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Alessio Meggiolaro, Sebastian Cremaschini, Davide Ferraro, Annamaria Zaltron, Mattia Carneri, Matteo Pierno, Cinzia Sada, Giampaolo Mistura
Summary: The actuation of droplets on a surface is crucial for microfluidic applications. One promising solution is to use iron-doped lithium niobate crystals to generate an evanescent electric field that controls the motion of water droplets. This study presents an experimental method to determine the attractive force exerted by the evanescent field and demonstrates the importance of these measurements for the design and characterization of optofluidic devices based on lithium niobate crystals.
Article
Chemistry, Analytical
Marco Morani, Myriam Taverna, Zuzana Krupova, Lucile Alexandre, Pierre Defrenaix, Thanh Duc Mai
Summary: This study presents a novel microfluidic droplet-based strategy for high-performance isolation of extracellular vesicles (EVs) using a magnetic bead-based approach combined with polyethylene glycol (PEG) without the use of antibodies. The reproducibility of EV recovery was improved, and isolated EVs identity was confirmed using capillary electrophoresis (CE) coupled with laser-induced fluorescent (LIF) detection. A purpose-made droplet platform was developed to further improve EVs capture efficiency and high throughput, achieving a significant improvement in recovery rate while reducing sample and reagent volumes and operation time.
Article
Chemistry, Analytical
Theo Lienard-Mayor, Camille Bricteux, Amel Bendali, Nguyet-Thuy Tran, Arnaud Bruneel, Myriam Taverna, Thanh Duc Mai
Summary: In this study, a new microfluidic droplet platform called Lab-in-Droplet is presented for multistep glycoprotein sample treatment. The platform enables full automation, droplet sensing and heating, allowing for the realization of several advanced functions such as heating and mixing with organic solvents. The Lab-in-Droplet platform demonstrated superiority over the conventional batchwise protocol, with reduced reagent consumption, shorter processing time, improved glycan labeling yield, and maintained glycan separation profile.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Bin Yang, Thanh Duc Mai, Nguyet Thuy Tran, Myriam Taverna
Summary: This study presents a new integrated approach for fluorescent labeling and analysis of N-glycans using capillary electrophoresis. The approach utilizes the capillary as both a microreactor for labeling and a separation channel for analysis, allowing for automation and improved efficiency. The method was successfully applied to analyze fluorescently labeled N-linked oligosaccharides from human immunoglobulin G and rituximab.
JOURNAL OF SEPARATION SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Caroline Twarog, Elias Fattal, Magali Noiray, Brigitte Illel, David J. Brayden, Myriam Taverna, Herve Hillaireau
Summary: This study compares the biophysical interactions between exenatide and C-10 or SNAC, finding that specific supramolecular structures are formed and non-specific interactions dominate the binding process. Bile salts in the gastrointestinal lumen inhibit the interaction between these permeation enhancers and exenatide.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Editorial Material
Biochemical Research Methods
Lucile Alexandre, Jiashu Sun, Myriam Taverna, Wenwan Zhong
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Alessio Meggiolaro, Valentina Moccia, Paola Brun, Matteo Pierno, Giampaolo Mistura, Valentina Zappulli, Davide Ferraro
Summary: Extracellular vesicles (EVs) are receiving considerable attention in the biological and medical fields due to their role as natural carriers of genetic information and their potential as diagnostic and prognostic biomarkers in various diseases. However, conventional methods for EV isolation have limitations, and microfluidic approaches have been proposed as a solution. This review summarizes the most important microfluidic-based devices for EV isolation and their advantages and disadvantages compared to existing technology.
Article
Chemistry, Analytical
Ngoc Van Thanh Nguyen, Claire Smadja, Myriam Taverna, Lac Thuy Huu Nguyen, Stephanie Descroix, Thanh Duc Mai
Summary: This study introduces a new concept of on-line dual preconcentration stages for capillary electrophoresis (CE), allowing two different preconcentration approaches to be used in the same capillary. The first stage involves dynamic magneto-extraction of target analytes using circulating magnetic beads, while the second stage utilizes electrokinetic preconcentration to focus the eluted analytes into a nano band before CE separation. A purpose-made instrument combining electrophoretic and microfluidic modules is used to control the movement of magnetic beads and analyte flow. The potential of this new enrichment principle and instrument is demonstrated for CE separation of target double-stranded DNA (ds-DNA) using light-emitting-diode-induced fluorescent (LEDIF) detection. The dual-stage preconcentration allows for in-capillary sample pretreatment and CE separation without mismatch of working volumes or waste of pretreated samples.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Joanie Otin, N. Thuy Tran, Aurelie Benoit, Corinne Buisson, Myriam Taverna
Summary: This study proposes a capillary electrophoresis method to analyze misused growth hormone-releasing hormone (GHRH) analogs. The use of dimethyl-beta-cyclodextrin as a chiral selector allows the separation of basic peptide analogs, including enantiopeptides that differ by one amino acid chirality. Electrokinetic preconcentration methods were investigated to increase sensitivity. The combination of preconcentration with chiral capillary zone electrophoresis successfully separates GHRH analogs with detection limits ranging from 75 to 200 ng/mL.
Article
Chemistry, Analytical
Maria Poles, Alessio Meggiolaro, Sebastian Cremaschini, Filippo Marinello, Daniele Filippi, Matteo Pierno, Giampaolo Mistura, Davide Ferraro
Summary: Magnetic beads are widely used in biochemical assays for purification and quantification of cells, nucleic acids, or proteins. However, their use in microfluidic devices is limited by precipitation due to their size and density. In this study, a shaking device is developed to prevent the sedimentation of magnetic beads, allowing for equal distribution in droplets without affecting droplet generation.
Article
Chemistry, Analytical
Ngoc Van Thanh Nguyen, Claire Smadja, Myriam Taverna, Trong Khoa Mai, Frederic Halgand, Thanh Duc Mai
Summary: A new method for studying the orientation and density of mouse antibody grafting on magnetic beads, which can be used in immunoassays and biosensors with fluorescent detection of biomolecules, is reported in this study. The method involves selective enzymatic digestion to obtain F(ab')2 and Fc fragments, followed by separation and determination of these fragments using size exclusion chromatography (SEC) coupled with fluorescence detection (FLD). The developed method was used to evaluate the grafting capacity of immunoglobulin (IgG2a) on different biofunctionalized magnetic beads, and the results show that tosyl-activated and protein G beads have superior grafting capacity compared to carboxylic beads at the optimal IgG:bead ratios. Under optimized conditions, more than 70% of antibodies were grafted onto tosyl-activated and protein G beads in the correct orientation. The method was then demonstrated with commercially available antibodies specific to amyloid-beta peptide 1-42 (A beta 1-42) for magneto-immunoassays and fluorescent detection of this peptide, which is an established biomarker for the molecular diagnosis of Alzheimer's disease.
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
