Article
Biochemical Research Methods
Shuai Yuan, Yulin Zhang, Lang Nan, P. T. Lai, Tong Zhang, Philip W. T. Pong, Ho Cheung Shum
Summary: Microbes are widely present in our environment and have significant impacts on our society. The treatment of microorganisms has always been a complex problem due to a lack of efficient screening tools. In this study, a novel all-aqueous microfluidic workflow is proposed to overcome the limitation of conventional systems. This approach allows for cell-cell and cell-environment interactions, promoting the growth of bacteria and enabling on-chip manipulation of microcapsules. The feasibility of this approach is demonstrated by single bacterium culturing in droplet-templated alginate beads and it offers new opportunities for microbial applications.
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Zongjie Wang, Hansen Wang, Sichun Lin, Sharif Ahmed, Stephane Angers, Edward H. Sargent, Shana O. Kelley
Summary: Magnetic cell sorting is a crucial tool for isolating specific cellular subpopulations, and a new approach has been developed to efficiently amplify the magnetic labeling on cells, enabling its application in cancer diagnostics and immunotherapy.
Article
Materials Science, Multidisciplinary
Matthew Lindley, Julia Gala de Pablo, Jorgen Walker Peterson, Akihiro Isozaki, Kotaro Hiramatsu, Keisuke Goda
Summary: Cell sorting is an important tool in biological research and medicine. Raman-activated cell sorting (RACS) has gained attention for its ability to discriminate cells based on their intracellular chemical content without the need for labeling. However, the widespread use of RACS has been limited by a trade-off between throughput and measurement bandwidth. In this study, this trade-off is overcome and broadband RACS in the fingerprint region with a record high throughput is demonstrated. Real-time label-free sorting of microalgal cells based on their accumulation of carotenoids and polysaccharide granules is also showcased.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
Robert Salomon, Sajad Razavi Bazaz, Wenyan Li, David Gallego-Ortega, Dayong Jin, Majid Ebrahimi Warkiani
Summary: This paper presents a detailed method that utilizes flow cytometry for quantitatively characterizing the performance of continuous-flow microfluidic devices designed for particle separation. This method overcomes the limitations of current methods such as high-speed fluorescent imaging or cell counting, as it accurately assesses device performance in complex, high concentration mixtures. It uniquely takes advantage of pulse processing in flow cytometry to quantify cell separation efficiencies and resulting sample purities, including both single cells and cell clusters. Additionally, it can be combined with cell surface phenotyping to measure separation efficiencies and purities in complex cell mixtures.
Article
Chemistry, Analytical
Claudia Zielke, Adriana J. Gutierrez Ramirez, Kelsey Voss, Maya S. Ryan, Azam Gholizadeh, Jeffrey C. Rathmell, Paul Abbyad
Summary: This study presents a label-free, fixation-free and passive sorting method to isolate activated T-cells shortly after activation and prior to the display of activation surface markers. The method uses a sorting platform called Sorting by Interfacial Tension (SIFT) based on pH. It successfully isolates and enriches activated T-cells on chip, and allows for the testing of T-cell activation modulators and the determination of differentiation outcomes.
Article
Nanoscience & Nanotechnology
Meng-Jie Shen, Rene C. L. Olsthoorn, Ye Zeng, Thomas Bakkum, Alexander Kros, Aimee L. Boyle
Summary: The article presents a new MACS method based on coiled-coil peptide interactions, enabling efficient cell sorting of different cell lines without the need for antigen presentation, and allowing for easy detachment of magnetic particles from cells after sorting.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Chemistry, Analytical
Malihe Farasat, Ehsan Aalaei, Saeed Kheirati Ronizi, Atin Bakhshi, Shaghayegh Mirhosseini, Jun Zhang, Nam-Trung Nguyen, Navid Kashaninejad
Summary: Separation and detection of cells and particles in a suspension are essential for various applications, including biomedical investigations and clinical diagnostics. Microfluidics has enabled the miniaturization of analytical devices by controlling the motion of fluid in micrometer-sized channels and chambers. Among different microfluidic methods, dielectrophoresis (DEP) offers advantages for manipulating neutral bioparticles using unique dielectric characteristics. This review focuses on signal-based DEP methods that utilize signal parameters such as frequency, amplitude, phase, and shape for cell/particle separation and manipulation.
Review
Chemistry, Analytical
Kangfu Chen, Zongjie Wang
Summary: Rare cells are important in the development and progression of diseases, making their analysis of great interest. Micro-magnetofluidic systems have emerged as a rapidly developing platform for the sensitive and cost-effective analysis of rare cells. This mini review provides a timely summary of the progress in this field, discussing the concepts, realizations, and applications of micro-magnetofluidic devices in rare cell analysis. The challenges in the development and translation of these devices into the clinic are also highlighted.
Article
Engineering, Biomedical
Jianwei Zhong, Minhui Liang, Qiang Tang, Ye Ai
Summary: By introducing a label-free selectable cell quantity encapsulation in droplets sorting system, the purity and throughput of single-cell droplets can be improved. This system combines electrical impedance based screening with biocompatible acoustic sorting to achieve high efficiency and throughput while removing multi-cells and empty droplets.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Analytical
Huaying Chen, Haixu Meng, Zhenlin Chen, Tong Wang, Chuanpin Chen, Yonggang Zhu, Jing Jin
Summary: This paper presents a microfluidic device for size sorting and in situ clonal culture of single cells. Experimental results show that the device can successfully sort the cells into different size ranges and perform time-lapse imaging during in situ culture.
Article
Chemistry, Analytical
Sitian He, Jinlan Wei, Lihua Ding, Xiaonan Yang, Yongjun Wu
Summary: This review describes the isolation and detection strategies of circulating tumor cells (CTCs) and highlights potential directions for future research and development by analyzing the challenges faced by current strategies. With advances in CTCs analysis technologies in recent years, the yield and efficiency of CTCs enrichment have gradually improved, as well as detection sensitivity.
Article
Chemistry, Multidisciplinary
Fatima Ezahra Chrit, Abhishek Raj, Katherine M. Young, Nicholas E. Stone, Peter G. Shankles, Kesiharjun Lokireddy, Christopher Flowers, Edmund K. Waller, Alexander Alexeev, Todd Sulchek
Summary: The study utilized a microfluidic device as a high-sensitivity sensor to accurately detect cell viability by transducing cell biomechanics to separation. Different angles of the ridges were evaluated to optimize cell separation and identification. Furthermore, the use of principal component analysis improved the accuracy of cell state identification by clustering populations with little overlap between viable and nonviable cells, enabling efficient cell sorting and label-free viability sensing.
Article
Chemistry, Analytical
Rana Altay, Murat Kaya Yapici, Ali Kosar
Summary: This study combines spiral microchannels with surface acoustic waves technology to achieve the separation and enrichment of circulating tumor cells from red and white blood cells. By implementing a two-stage process, cells are focused and collected at different outlets based on their physical properties, resulting in efficient separation.
Article
Chemistry, Analytical
Peixian Li, Ye Ai
Summary: Biophysical markers of cells have shown promise in studying, characterizing, and classifying different cell types. The lack of cell sorting ability based on multivariate biophysical markers presents a challenge in isolating specific cell types. This study demonstrates label-free and high-throughput acoustic single-cell sorting activated by the characterization of multivariate biophysical phenotypes, providing a solution to the challenges of cell purification without specific molecular biomarkers.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Grant Korensky, Xinye Chen, Mengdi Bao, Abbi Miller, Blanca Lapizco-Encinas, Myeongkee Park, Ke Du
Summary: A planar, transparent, and adaptable nanosieve device has been developed for efficient separation of microalgae and bacteria. This device can trap almost all microalgae cells while allowing bacteria cells to pass through, and is capable of single-cell auto-fluorescence tracking. It is a valuable method for purifying microalgae samples containing contaminations and studying single-cell heterogeneity.
Review
Biochemical Research Methods
Joo H. Kang
Article
Optics
Taeseong Woo, Su Hyun Jung, Cheolwoo Ahn, Byungjae Hwang, Hyunggee Kim, Joo H. Kang, Jung-Hoon Park
Article
Multidisciplinary Sciences
Eujin Um, Minjun Kim, Hyoungsoo Kim, Joo H. Kang, Howard A. Stone, Joonwoo Jeong
NATURE COMMUNICATIONS
(2020)
Correction
Optics
Taeseong Woo, Su Hyun Jung, Cheolwoo Ahn, Byungjae Hwang, Hyunggee Kim, Joo H. Kang, Jung-Hoon Park
Article
Chemistry, Multidisciplinary
Seyong Kwon, Jieung Oh, Min Seok Lee, Eujin Um, Joonwoo Jeong, Joo H. Kang
Summary: A hemolysis-free and highly efficient plasma separation platform has been developed using enhanced diamagnetic repulsion of blood cells. By supplementing blood with superparamagnetic iron oxide nanoparticles, complete removal of blood cells from blood plasma is achieved, allowing for successful collection of plasma without losing plasma proteins, platelets, or exosomes.
Article
Biochemistry & Molecular Biology
Seon Yong Lee, Sang-Hun Choi, Min Seok Lee, Amanzhol Kurmashev, Hae Nim Lee, Young-Gyu Ko, Kanghun Lee, Sohee Jeong, Jihye Seong, Joo H. Kang, Hyunggee Kim
Summary: Glioblastoma (GBM) is a highly infiltrative and refractory disease, with the perivascular niche (PVN) being described as a route of dissemination. Retraction fibers (RFs) formed by perivascular extracellular matrix proteins play a crucial role in GBM cell motility, regulated by integrin alpha 5 beta 1. GBM cells utilize these structures for migration, with infiltrative characteristics potentially attributed to interactions between cells.
Article
Engineering, Biomedical
Brian Choi, Jeong-Won Choi, Hyungwon Jin, Hye-Rim Sim, Jung-Hoon Park, Tae-Eun Park, Joo H. Kang
Summary: This study presents a method to construct an MPS device by selectively condensing a submicrometer-thickness ECM fiber film on a large pore-sized TE membrane, achieving functional tissue barriers, optical transparency, intercellular interactions, and cell transmigration simultaneously. The cECMTE membrane allows for the formation of a monolayer of human brain endothelial cells in an MPS device and proficient maturation of the BBB. It also enables the study of intercellular communications and migration, mimicking physiological barriers of organ compartments.
Article
Immunology
Hwi Hyun, Min Seok Lee, Inwon Park, Hwa Soo Ko, Seongmin Yun, Dong-Hyun Jang, Seonghye Kim, Hajin Kim, Joo H. Kang, Jae Hyuk Lee, Taejoon Kwon
Summary: Recent studies have shown the presence of a blood microbiome in healthy hosts, with bacterial populations detected in the bloodstream even before infection in a porcine model of polymicrobial bacteremia. The native blood microbiome was different from the fecal microbiome, and new bacterial strains entered the bloodstream upon infection. Communication and stress management pathways are crucial for the survival of the blood microbiome.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Min Seok Lee, Hwi Hyun, Inwon Park, Sungho Kim, Dong-Hyun Jang, Seonghye Kim, Jae-Kyeong Im, Hajin Kim, Jae Hyuk Lee, Taejoon Kwon, Joo H. Kang
Summary: This study developed a novel technology called mu FISH, which uses mu FISH probes based on 16S rRNA sequences to quantitatively identify and detect bacteria in blood samples. It is a more sensitive method compared to traditional bacterial detection methods.
Article
Microbiology
Inwon Park, Dongsung Kim, Jae Hyuk Lee, Sung Jin Park, Hwain Jeong, Sumin Baek, Seonghye Kim, Serin Kim, Ji Eun Hwang, Hyuksool Kwon, Joo H. Kang, You Hwan Jo
Summary: Proper selection of susceptible antibiotics is critical in treating bloodstream infections caused by drug-resistant bacteria. This study aimed to evaluate the trends of hemodynamics and biomarkers in a porcine bacteremia model treated with insusceptible antibiotics compared to those in susceptible models. The results showed that the sTREM-1 level at 2 hours after antibiotics administration represented a significant difference in antibiotic susceptibility. Further clinical studies are needed for validation of sTREM-1 as a biomarker for in vivo antibiotic susceptibility.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Multidisciplinary
Su Hyun Jung, Bong Hwan Jang, Seyong Kwon, Sung Jin Park, Tae-Eun Park, Joo H. Kang
Summary: Autologous implantable scaffolds using autologous whole blood are effective in promoting skin wound healing by constructing robust microcapillary vessel networks. Microfluidic shear stresses enable the alignment of bundled fibrin fibers and the activation of platelets, facilitating endothelial cell maturation and vascularization. Rodent dorsal skin wounds patched with IVET exhibit superior wound closure rates, epidermis thickness, collagen deposition, hair follicle numbers, and neutrophil infiltration, attributed to enhanced microvascular circulation. Moreover, IVET treatment accelerates wound healing by recruiting M2 phenotype macrophages.
ADVANCED MATERIALS
(2023)
Proceedings Paper
Engineering, Electrical & Electronic
Taeseong Woo, Su Hyun Jung, Joo H. Kang, Jung-Hoon Park
2020 IEEE PHOTONICS CONFERENCE (IPC)
(2020)
Article
Biophysics
Seyong Kwon, Amanzhol Kurmashev, Min Seok Lee, Joo H. Kang
BIOSENSORS & BIOELECTRONICS
(2020)
Article
Biochemical Research Methods
Thao Nguyen, Su Hyun Jung, Min Seok Lee, Tae-Eun Park, Suk-kyun Ahn, Joo H. Kang
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)