Article
Spectroscopy
Yu Zhu Yang, Zi Yi Xu, Nian Bing Li, Hong Qun Luo
Summary: This study developed two new fluorescent probes for visual detection of esterase activity in cells and imaging in living cells. The probe VA showed higher sensitivity and lower detection limit. These probes have high biocompatibility and high spatial resolution, providing a reliable method for detecting endogenous esterase.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Spectroscopy
Bingpeng Guo, Tianjiao Shen, Yifan Liu, Jing Jing, Changxiang Shao, Xiaoling Zhang
Summary: Esterase is primarily distributed in the endoplasmic reticulum (ER) and often overexpressed in cancer cells. To solve the limitations of existing probes, an ER-specific ratiometric fluorescent probe (ER-EST) is designed for detecting esterase in living cells. The ER-EST shows a ratiometric and red-shifted emission from 435 to 560 nm after hydrolysis by esterase, and it has a low detection limit and good biocompatibility.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Chemistry, Multidisciplinary
Xiao Liu, Young-Tae Chang
Summary: This article outlines the concepts and mechanisms of different strategies for live cell discrimination and introduces their applications in cell-type discrimination. These strategies include protein-oriented, carbohydrate-oriented, DNA-oriented, gating-oriented, metabolism-oriented, and lipid-oriented approaches. Fluorescent probes can achieve live cell distinction by interacting with biomolecules, accumulating via transporters, and participating in metabolism.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Chemistry, Analytical
Wu-Yingzheng Guo, Yi-Xuan Fu, Shi-Yu Liu, Long-Can Mei, Yao Sun, Jun Yin, Wen-Chao Yang, Guang-Fu Yang
Summary: The study developed a strategy of multienzyme-targeted fluorescent probe design and obtained a novel fluorescent probe for detecting a wide variety of pesticides. The designed probe showed excellent sensitivity against organophosphorus and carbamate pesticides, with a detection limit of 1.14 pg/L for dichlorvos. It also allowed for the diffusion-resistant in situ visualization of pesticides in live cells and zebrafish, as well as the sensitive measurement of organophosphorus pesticides in fresh vegetables, demonstrating promising potential for tracking pesticide residues in environment and biological systems.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Multidisciplinary
Julia Ackermann, Justus T. Metternich, Svenja Herbertz, Sebastian Kruss
Summary: Biosensors are important tools in modern basic research and biomedical diagnostics. NIR fluorescent molecular sensors based on SWCNTs have great potential in analyte detection, and the research on chemical design strategies and molecular recognition contributes to their further development.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Xin Zhang, Ying Chen, Haisheng He, Shangfeng Wang, Zuhai Lei, Fan Zhang
Summary: This study introduces a dual activatable fluorescent probe PN910, which shows high selectivity toward H2O2 and OONO- at pH beyond 7.4 in alkaline tissue. It can be used for real-time, specific, and accurate monitoring of cystitis and colitis in living animals, presenting a unique approach for in vivo biosensing with a dual activatable probe.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Amanpreet Singh, Sanjeev Saini, Mayank, Navneet Kaur, Ajnesh Singh, Narinder Singh, Doo Ok Jang
Summary: An anion-mediated preorganization approach was used to design and synthesize a benzimidazolium-based calix compound R1.2 ClO4-, which was able to encapsulate DMF molecules. A nanoreactor was fabricated by using R1.2 ClO4- and l-histidine as components, showing detoxification ability for paraoxon within 30 minutes, with l-His playing a vital role in the process. The nanoreactor inhibited AChE activity, as determined by Ellman's reagent.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Xue Tian, Lloyd C. Murfin, Luling Wu, Simon E. Lewis, Tony D. James
Summary: Small-molecule based fluorescent probes play an increasingly important role in detecting and imaging biological signaling molecules due to their simplicity, high selectivity, and real-time analysis capabilities. Various sensing mechanisms, including FRET, ICT, PeT, ESIPT, AIE, as well as dual/triple sensing mechanisms, are highlighted, but challenges remain. Future development should focus on improving small-molecule fluorescent probes for biosensing applications.
Article
Chemistry, Analytical
Haifeng Yang, Chengmei Liu, Yu Mei, Peng Zhang, Qian Zhang, Caifeng Ding
Summary: A novel near-infrared fluorescent probe P1 was developed for ratiometric monitoring of esterase activity. It showed high sensitivity and selectivity for esterase, and achieved precise quantitative analysis and imaging in living cells and in vivo.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Qiang Liu, Mei Liu, Yan Jin, Baoxin Li
Summary: Fluorescence biosensing based on CRISPR/Cas12a system with ratiometric probe as the reporter exhibits higher sensitivity compared to traditional TaqMan probe, and it can distinguish and avoid false positive signals.
Article
Chemistry, Analytical
Shi-Yu Liu, Xiaoting Zou, Xing Gao, Yue-Yang Zhang
Summary: A novel nonpeptide-based fluorogenic probe named TMBIHF was designed and synthesized for specific chymotrypsin activity sensing. By substituting the ester-based linker with a heptafluorobutylamide moiety, the acquired probe showed sufficient stability and specificity for chymotrypsin detection. It exhibited high selectivity towards various enzymes and reactive chemicals, while maintaining high sensitivity and catalytic efficiency towards chymotrypsin. The probe was successfully applied for monitoring chymotrypsin activity and pancreas development in live zebrafish, specific sensing of exogenous and endogenous chymotrypsin in nude mice, and visualizing chymotrypsin-like activity-dependent cellular apoptosis.
ANALYTICAL CHEMISTRY
(2022)
Review
Polymer Science
Yanan Zhang, Dajun Hou, Zelong Wang, Ning Cai, Chaktong Au
Summary: This review systematically summarizes the recent progress in nanomaterial-based dual-emission ratiometric fluorescent biosensors, which play an essential role in biosensing and cell imaging applications. It introduces general design approaches and typical examples, while also discussing potential challenges and future outlook for dual-emission ratiometric fluorescent nanosensors.
Article
Chemistry, Multidisciplinary
Jie Li, Weize Yuan, Shao-Xiong Lennon Luo, Mate J. Bezdek, Alexander Peraire-Bueno, Timothy M. Swager
Summary: We report a wireless lateral flow device that converts enzyme-catalyzed reactions into changes in radio frequency identification (RFID) circuit resonance for detecting biological signals. This method enables rapid, portable, and quantitative detection of biological targets.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Yingcui Bu, Mengtao Rong, Junjun Wang, Xiaojiao Zhu, Jie Zhang, Lianke Wang, Zhipeng Yu, Yupeng Tian, Hongping Zhou, Yi Xie
Summary: This study developed a novel molecule MO, which can lastingly anchor onto the plasma membrane and reflect membrane damage and heterogeneity by secretion of extracellular vesicles.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yaguang Yin, Xiuqi Kong, Min Li, Jingchao Wang, Xiaoyu Dai, Yunyan Zhang, Weiying Lin
Summary: In this study, a novel fluorescent probe Y-1 was designed for monitoring esterase in living cells. Probe Y-1 showed distinct fluorescence when bound with esterase, demonstrating high sensitivity, fast response, and high selectivity. The application of this probe in living cells suggests its potential importance in detecting esterase.
ANALYTICA CHIMICA ACTA
(2022)
Review
Oncology
Marianna Aprile, Valerio Costa, Amelia Cimmino, George Adrian Calin
Summary: The view of long noncoding RNAs as nonfunctional garbage has been outdated, as evidence suggests their role as golden junk, particularly in precision oncology. They have potential use as diagnostic and/or prognostic biomarkers, and advancements in technology have made it easier to profile them in multiple tumors and large cohorts.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Allergy
Federica Pulvirenti, Eva Piano Mortari, Carolina Putotto, Sara Terreri, Ane Fernandez Salinas, Bianca Laura Cinicola, Eleonora Cimini, Giulia Di Napoli, Eleonora Sculco, Cinzia Milito, Paolo Versacci, Chiara Agrati, Bruno Marino, Rita Carsetti, Isabella Quinti
Summary: This study analyzed the relationship between immune defects and heart diseases in patients with 22q11.2 deletion syndrome (22q11.3DS) and the outcome of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The study found that most patients with 22q11.2DS had a mild course of SARS-CoV-2 infection, and vaccination induced specific antibody and memory cell responses.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Chemistry, Multidisciplinary
Fabio Pirro, Salvatore La Gatta, Federica Arrigoni, Antonino Famulari, Ornella Maglio, Pompea Del Vecchio, Mario Chiesa, Luca De Gioia, Luca Bertini, Marco Chino, Flavia Nastri, Angela Lombardi
Summary: De novo metalloprotein design is a remarkable method for shaping protein structures to achieve specific functions. In this study, a protein called DR1 was designed and characterized to harbor a di-copper site, mimicking copper-containing polyphenol oxidases. The di-copper site was engineered into a four-helix bundle scaffold through hierarchical design of metal coordination spheres. Spectroscopic, thermodynamic, and functional analyses demonstrated that DR1 exhibited the characteristics of a Type 3 copper site, supporting different copper redox states and catalyzing the oxygen-dependent oxidation of catechols. Careful design of substrate access site residues endowed DR1 with substrate recognition capability.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Michela Rossi, Viviana De Martino, Laura Di Giuseppe, Giulia Battafarano, Jacopo Di Gregorio, Sara Terreri, Francesco Marampon, Salvatore Minisola, Andrea Del Fattore
Summary: The effects of the histone deacetylase inhibitor PXD-101 on human osteosarcoma cells were investigated. The study found that PXD-101 inhibited cell proliferation, induced apoptosis, and inhibited cell migration. This study suggests that PXD-101 may be a potential new therapeutic approach for osteosarcoma patients.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Chemistry, Inorganic & Nuclear
Rosa Bellavita, Linda Leone, Angela Maione, Lucia Falcigno, Gabriella D'Auria, Francesco Merlino, Paolo Grieco, Flavia Nastri, Emilia Galdiero, Angela Lombardi, Stefania Galdiero, Annarita Falanga
Summary: Ferric iron is necessary for bacterial growth. Pathogenic bacteria use siderophores to chelate ferric iron and colonize host organisms. Conjugating antimicrobial peptides with iron chelators can reduce iron availability and enhance the efficacy of AMPs.
DALTON TRANSACTIONS
(2023)
Review
Biochemistry & Molecular Biology
Fabiana Tortora, Evelina La Civita, Pankaj Trivedi, Ferdinando Febbraio, Daniela Terracciano, Amelia Cimmino
Summary: Renal cell carcinoma, bladder cancer, and prostate cancer are the most common genitourinary tumors. Recent advancements in understanding oncogenic factors and molecular mechanisms have greatly improved their treatment and diagnosis. Non-coding RNAs, such as microRNAs, long non-coding RNAs, and circular RNAs, have been found to play important roles in the occurrence and progression of genitourinary cancers. Interactions of DNA, protein, and RNA with lncRNAs and other biological macromolecules contribute to cancer phenotypes. This review focuses on the mechanisms of abnormal lncRNA expression in genitourinary tumors and their potential applications in diagnostics, prognosis, and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Linda Leone, Gianmattia Sgueglia, Salvatore La Gatta, Marco Chino, Flavia Nastri, Angela Lombardi
Summary: The potential of hydrogen as a clean and sustainable fuel has led to the search for environmentally friendly methods of production, with biological catalysts being the most attractive solution. However, limitations in the production and stability of hydrogenases have hindered their use in scalable hydrogen production. Inspired by nature, significant efforts have been made in developing artificial systems that can mimic the catalytic function of hydrogenases. This review provides an overview of hydrogenases and their integration in devices for hydrogen and energy production, as well as the recent advances in the development of homogeneous hydrogen evolution catalysts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nursing
Annamaria D'Alessandro, Anna Pastore, Patrizia Amadio, Matteo D'Agostini, Sara Terreri, Rita Carsetti, Marta Argentieri, Paola Bernaschi, Andrea Onetti Muda, Ottavia Porzio, Andrea Dotta, Guglielmo Salvatori
Summary: This study aimed to reduce milk fat content without affecting other nutrients, oxidative stress, or introducing harmful microorganisms. The results showed that low-temperature centrifugation was very effective in reducing triglyceride concentration by 85% and cholesterol by 50%. Additionally, the procedure did not significantly affect other components, oxidative stress markers, or introduce harmful microorganisms.
JOURNAL OF HUMAN LACTATION
(2023)
Article
Biochemistry & Molecular Biology
Linda Leone, Ana Belen Munoz-Garcia, Daniele D'Alonzo, Vincenzo Pavone, Flavia Nastri, Angela Lombardi
Summary: In the past few decades, efforts have been made to construct protein and peptide-based metalloporphyrin catalysts for selective and difficult transformations. Mechanistic studies are crucial to understand the factors influencing catalytic performances and product selectivity. Our previous work demonstrated the efficiency of the synthetic peptide-porphyrin conjugate MnMC6*a as a catalyst for indole oxidation. In this study, we investigated the role of the metal ion by replacing manganese with iron and found that although product selectivity remained unchanged, FeMC6*a showed lower substrate conversion and prolonged reaction times compared to MnMC6*a.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Marco Chino, Luigi Franklin Di Costanzo, Linda Leone, Salvatore La Gatta, Antonino Famulari, Mario Chiesa, Angela Lombardi, Vincenzo Pavone
Summary: Designing metal sites into de novo proteins has made significant progress recently. However, identifying the minimal coordination spheres for metal binding and activity remains a challenge. This study successfully assembled essential elements into a small protein, allowing it to fold around a metal center and function in electron-transfer. The crystal structure of the designed metal-binding protein matched the intended design, enabling correlation between structure and properties.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Attila Tortorella, Linda Leone, Angelina Lombardi, Elio Pizzo, Andrea Bosso, Roland Winter, Luigi Petraccone, Pompea Del Vecchio, Rosario Oliva
Summary: The misuse of antibiotics has resulted in drug-resistant pathogens. Antimicrobial peptides (AMPs) could serve as alternatives to antibiotics, but their susceptibility to degradation restricts their usage. Different strategies have been developed to overcome this issue, and glycosylation of AMPs is a promising approach.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Valentina De Luca, Luigi Mandrich, Giuseppe Manco
Summary: In recent decades, the increased use of pesticides in agriculture has led to safety concerns for the environment and human health, necessitating the development of new detection and decontamination systems. A new qualitative test utilizing thermostable enzymes was discovered, allowing for the detection of pesticide residues on fruits and vegetables. By analyzing esterase activity, the presence of residual pesticides and their decontamination was successfully assessed.
Editorial Material
Biochemistry & Molecular Biology
Linda Leone, Flavia Nastri, Angela Lombardi
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)