Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 267, Issue -, Pages 357-365Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.03.185
Keywords
Cell apoptosis; Caspese-3; Calixarenes; Electrochemical detection; Graphene oxide
Funding
- National Natural Science Foundation of China [61275085]
- State Key Laboratory of Dairy Biotechnology of China [SKLDB2016-001]
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Apoptosis is a crucial event for the efficacy of anticancer drug. Caspase-3 plays a critical role in cell signaling pathways of apoptosis. The calixarene derivatives display high affinity for various kinds of biomolecules by host-guest recognition own to their unique cavity structure. The calixarene functionalized reduction of graphene oxide exhibits stronger molecular recognition ability and a higher electrochemical response to biomolecules than unmodified graphene oxide. In this work, caspase-3 recognizes and cleaves N-terminal blocked peptide containing tetra-peptide substrate Asp-Glu-Val-Asp. p-sulfonatocalix[6]arenes sodium modified graphene oxide (pSC(6)-rGO) recognizes exposed N-terminal amine group assembled on the electrode. Due to the large surface area to volume ratio of rGO, numerous electrochemical active methylene blues (MB) can be absorbed through host-guest recognition. As a result, sensitive caspase-3 detection was achieved with a low detection limit of 0.0167 pg/mL by this electrochemical signal amplification strategy. This approach was also applied to measure apoptosis in the practical cell samples and shows good performance. (C) 2018 Elsevier B.V. All rights reserved.
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