Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 262, Issue -, Pages 1024-1033Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.02.086
Keywords
Lab-on-chip; Loop-mediated isothermal amplification; LAMP; Bioluminescent assay in real time BART; Viral nucleic acids detection; Amorphous silicon sensors; Thin film technology
Funding
- Italian Ministry of Education, University and Research (MIUR) through PRIN [20108ZSRTR]
- MIUR through the University Research Project [prot. C26H15J3PX]
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The present paper describes the development of an integrated lab-on-chip, in which viral DNA amplification with real-time on-chip detection is carried out under constant temperature of 65 degrees C. The lab-on-chip is composed of a disposable 10-pi polydimethylsiloxane reaction chamber which is thermally and optically coupled to a glass substrate that hosts a thin-film metallic resistive heater and thin-film amorphous silicon diodes which act as temperature and radiation sensors. A loop-mediated isothermal amplification (LAMP) technique was optimized to specifically amplify parvovirus B19 DNA and coupled with Bioluminescent Assay in Real Time (BART) technology to provide real-time detection of target DNA. The experimental results demonstrate the ability of the proposed device to discriminate among different concentrations of viral DNA with an excellent agreement with standard off-chip methods. (C) 2018 Elsevier B.V. All rights reserved.
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