Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 263, Issue -, Pages 417-425Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.02.095
Keywords
Unlabeled detection; Living cell responses; Graphene; Biosensor; Whole cells
Funding
- Natural Science Foundation of China [61505109, 81472729, 81672426]
- National Basic Research Program of China [2016YFC0102404]
- Foundation of Committee on Science and Technology of Tianjin [15JCQNJC42200]
- Science and Technology Plan Program of Beijing [Z17010101247]
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It has been challenging to measure responses of unlabeled, live whole cells. In this work, we designed a graphene-based optical biosensor with a detection depth of greater than 2 mm, a high sensitivity of 1.2 x 10(8) mV/RIU, and a fast response time of 260 ns. This novel biosensor enables fast and highly accurate label-free detection of drug responses in live cells at the cell height level, and can even sense cancer cell responses to anti-cancer drugs. Unexpected early responses to paclitaxel were observed in cancer cells using this biosensor. Two colorectal cancer cell lines, HCT116 and LoVo, were used for measurement. By combining the results from conventional biological assays, including MTT, western blot and immunofluorescence assays, these novel responses were examined in detail. This facile biosensor shows promise to be widely used as a sensor of unlabeled live cell responses for cancer therapy, anti-cancer drug screening and other cell-based research. (C) 2018 Elsevier B.V. All rights reserved.
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