Article
Computer Science, Interdisciplinary Applications
P. Perez, J. A. Serrano, M. E. Martin, P. Daza, G. Huertas, A. Yufera
Summary: A computer program is developed for the system design of biosensors applied to monitoring cell culture dynamics. The program allows for the confident obtaining of system information through electrical stimulation, accurately predicting the output frequency and amplitude ranges of the voltage response. Deep insight information on cell size, number, and time-division can be extracted from comparing with real cell culture assays in the future.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Article
Chemistry, Analytical
Juan Alfonso Serrano, Pablo Perez, Paula Daza, Gloria Huertas, Alberto Yufera
Summary: Obtaining cell concentration measurements from a culture assay using bioimpedance is a useful method. This study aimed to find a method to obtain real-time cell concentration values by using an oscillator as the measurement circuit. Models were derived to estimate cell concentration in real time by using the oscillation frequency and amplitude. Real-time data of the cell concentration were obtained and compared to concentration data obtained by traditional optical methods.
Article
Physiology
Lin Yang, Meng Dai, Qinglin Cao, Shuai Ding, Zhanqi Zhao, Xinsheng Cao, Zhihong Wen, Hang Wang, Manjiang Xie, Feng Fu
Summary: This study demonstrated that electrical bioimpedance (EBI) can sensitively and accurately detect the onset of hypoxia in real time, showing a significant correlation and high concordance with blood oxygen saturation (Sp(O2)).
JOURNAL OF APPLIED PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nadja Engel, Michael Dau, Vivien Engel, Denise Franz, Fabian Klemmstein, Christiane Thanisch, Juergen F. Kolb, Marcus Frank, Armin Springer, Ruediger Koehling, Rainer Bader, Bernhard Frerich, Nadine Wiesmann, Diana Heimes, Peer W. Kaemmerer
Summary: By combining electrical stimulation of a commercial titanium implant with real-time impedance sensing, osteogenesis progress can be monitored and electrical stimulation can be adjusted accordingly for individual fracture healing.
Article
Cell Biology
Ineke D. C. Jansen, Thijs van Velzen, Teun J. de Vries, Robert Szulcek, Jack J. W. A. van Loon
Summary: In this study, an impedimetric bioassay was introduced for real-time monitoring of osteoclast resorption. The method involves culturing osteoclasts on a calcium phosphate substrate with electrodes and applying different chemical stimuli to measure their activity continuously. The results showed that inflammatory factors and bisphosphonates significantly affected osteoclastic activity. This method is of great importance for studying osteoclast function and diseases, as well as for high-throughput drug testing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Andrzej Kociubinski, Aleksandra Wilczynska, Pawel A. Mazurek, Dominika Pigon-Zajac, Teresa Malecka-Massalska, Monika Prendecka-Wrobel
Summary: This paper explores the possibility of using impedance measurement to monitor morphological changes in cell culture in the presence of an electromagnetic field. The study used Electric Cell-Substrate Impedance Sensing (ECIS) method to study cell behaviors. A device that could be connected in a climatic chamber was used to test mouse fibroblast culture. The results showed little effect of the electromagnetic field on cell culture, and the observed changes in impedance, resistance, and capacitance were likely due to the separation of positive and negative ions in the field.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Analytical
Fuentes-Velez Susana, Fagoonee Sharmila, Sanginario Alessandro, Gallo Valentina, Riganti Chiara, Pizzi Marco, Altruda Fiorella, Demarchi Danilo
Summary: Interest in impedance-based cellular assays is growing due to their multiple advantages, and our study demonstrates the potential of ECIS in cancer treatment decisions and early detection of chemoresistance. The analysis of impedance-based curves distinguishes between chemo-sensitive and chemo-resistant cells, and promises the study of time-dependent factors related to resistance acquisition in cells. The continuous monitoring and highthroughput early detection of drug resistance using the ECIS device presents new possibilities for cancer treatment and drug screening.
Article
Chemistry, Multidisciplinary
Yu Qin, Xue-Bo Hu, Wen-Ting Fan, Jing Yan, Shi-Bo Cheng, Yan-Ling Liu, Wei-Hua Huang
Summary: A stretchable and multifunctional platform integrating 3D cell culture, mechanical loading, and electrochemical sensing has been developed for the first time, showing promising potential in exploring mechanotransduction in 3D cellular systems and engineered tissues. The platform utilizes biomimetic peptide linked gold nanotubes on porous and elastic polydimethylsiloxane to mimic articular cartilage and investigate mechanotransduction pathways in chondrocytes. The results reveal previously unclear mechanisms, such as the rapid activation of nitric oxide signaling by mechanical loading in chondrocytes.
Review
Pediatrics
Roisin O'Neill, Eugene M. Dempsey, Aisling A. Garvey, Christoph E. Schwarz
Summary: Circulatory monitoring in neonatal care is currently limited to heart rate and blood pressure assessment, but non-invasive cardiac output monitoring (NiCO) is gaining popularity in term and preterm neonates. This narrative review summarized 33 studies involving nearly 2,000 neonates and focused on the interchangeability with echocardiography. The review also discussed the application of NiCO in various clinical settings and highlighted the need for future research goals.
FRONTIERS IN PEDIATRICS
(2021)
Article
Engineering, Biomedical
Adnan Albaba, Ivan Castro, Pascal Borzee, Bertien Buyse, Dries Testelmans, Carolina Varon, Sabine Van Huffel, Tom Torfs
Summary: The objective of this study was to design an algorithm for classifying ccBioZ segments based on signal quality to enhance confidence in extracted respiratory activity monitoring information, such as respiration rate. This was achieved through extracting and selecting features to find the best balance between classifier performance and the number of features used. Testing the algorithm on three datasets from 12 subjects showed high accuracy, sensitivity, specificity, and balanced accuracy, indicating the reliability and robustness of the approach.
BIOMEDICAL SIGNAL PROCESSING AND CONTROL
(2021)
Article
Engineering, Biomedical
Kristine L. Richardson, Caitlin N. Teague, Samer Mabrouk, Brandi N. Nevius, Goktug C. Ozmen, Rachel S. Graham, Daniel P. Zachs, Adam Tuma, Erik J. Peterson, Hubert H. Lim, Omer T. Inan
Summary: This study demonstrates the ability to detect RA disease activity using noninvasive sensing, providing objective data for treating clinicians to improve assessment of disease activity without the need for face-to-face clinic visits.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2022)
Article
Horticulture
David Romero Fogue, Rafael Masot Peris, Javier Ibanez Civera, Laura Contat Rodrigo, Nicolas Laguarda-Miro
Summary: This study developed a fast, economic and easy-to-implement method for early freeze-damage detection in grapefruit using electric impedance spectroscopy and neural network models. The research analyzed different impedance responses and simulated electrical behavior to understand the biological changes caused by freezing.
Article
Computer Science, Information Systems
Alexia Bailleul, Florence Poulletier De Gannes, Antoine Pirog, Gilles N'kaoua, Adrien D'Hollande, Aurelian Houe, Fabien Soulier, Serge Bernard, Sylvie Renaud
Summary: This study presents a novel biological-hardware-software tool for evaluating the physiological condition of in vitro myotubes in response to electrical stimulation. The tool utilizes impedance spectroscopy, electrical stimulation, and optical microscopy to measure physiological changes and contractions of muscle cells. Through experiments with a muscle cell model, the functionality of the platform was validated, and a bioimpedance-based metric was defined to evaluate changes in myotubes' physiology with electrical stimulation.
Article
Urology & Nephrology
Rafael Edgardo Maldonado-Valadez, Erika Beatriz Falcon-Sandoval, Francisco Miguel Vargas-Luna, Svetlana Kashina, Jose Marco Balleza-Ordaz
Summary: This study evaluated the relationship between electrical bioimpedance and detrusor bladder pressure, and found a significantly high correlation between the two. The results need to be confirmed in a larger population.
INTERNATIONAL UROLOGY AND NEPHROLOGY
(2022)
Review
Chemistry, Physical
Haocheng Deng, Song Xiao, Aijun Yang, Haoying Wu, Ju Tang, Xiaoxing Zhang, Yi Li
Summary: The state perception of electrical power transmission and distribution equipment is crucial for the stability, digitalization, and intelligence of the power grid. Nanogenerators, with their environmental friendliness, flexible design, and high fusion properties, have shown outstanding performance in ambient micro-nano energy harvesting and self-powered online monitoring. They offer potential applications in the power IoT, particularly in transmission lines, transformers, and gas-insulated equipment.
Article
Computer Science, Interdisciplinary Applications
P. Perez, J. A. Serrano, M. E. Martin, P. Daza, G. Huertas, A. Yufera
Summary: A computer program is developed for the system design of biosensors applied to monitoring cell culture dynamics. The program allows for the confident obtaining of system information through electrical stimulation, accurately predicting the output frequency and amplitude ranges of the voltage response. Deep insight information on cell size, number, and time-division can be extracted from comparing with real cell culture assays in the future.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Review
Nutrition & Dietetics
Elena Grao-Cruces, Lourdes M. Varela, Maria E. Martin, Beatriz Bermudez, Sergio Montserrat-de la Paz
Summary: The Mediterranean diet has favorable effects on HDL functionality, improving HDL cholesterol efflux capacity and decreasing HDL oxidation. Additionally, the diet influences HDL composition and size, making it a protective factor against cardiovascular disease by improving HDL quality and preventing HDL dysfunctionality.
Review
Biochemistry & Molecular Biology
Elena Grao-Cruces, Soledad Lopez-Enriquez, Maria E. Martin, Sergio Montserrat-de la Paz
Summary: High-density lipoproteins (HDLs) are heterogeneous lipoproteins that can modify their composition and functions depending on physiological or pathological conditions. Their main roles include cholesterol efflux, anti-inflammatory, and antioxidant functions, but may be compromised under pathological conditions. HDLs play a role in the immune system as anti-inflammatory molecules, but their composition changes when inflammation occurs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Physical
Paula Navarro, Alberto Olmo, Merce Giner, Marleny Rodriguez-Albelo, Angel Rodriguez, Yadir Torres
Summary: This study investigates a non-invasive alternative method, electrical impedance spectroscopy, for characterizing the chemical composition and surface topography of titanium implants. The effects of femtosecond laser treatment and cell culture on titanium discs are also examined. Results show that this measurement technique has the potential to quantitatively analyze porosity and surface modification effects.
Review
Food Science & Technology
Elena M. Grao-Cruces, Sergio Montserrat de la Paz, Maria E. Martin
Summary: Moderate beer consumption is associated with beneficial effects on cardiovascular health, while excessive intake should be avoided. The components in beer, such as alcohol and phenolics, influence lipid profile in blood, modulating cardiovascular risk.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Food Science & Technology
Carmen M. Claro-Cala, Elena Grao-Cruces, Rocio Toscano, Maria C. Millan-Linares, Sergio Montserrat-de la Paz, Maria E. Martin
Summary: Phytol from hemp seed oil has anti-inflammatory activity and can alter the plasticity of human monocyte-macrophages, reducing inflammatory response.
Article
Chemistry, Analytical
Antonio Rodriguez, Pablo Barroso, Alberto Olmo, Alberto Yufera
Summary: Assessing the viability of bioimpedance sensing to detect atheromatous plaque formation in implantable stents was the focus of this study. Simulations in COMSOL Multiphysics were performed using both non-pathological and pathological models, taking into account the flow of blood. The results showed that the proposed bioimpedance sensing system could successfully detect the presence of atheromatous plaque based on the measured current intensity in the electrodes. This simple algorithm approach eliminates the need for complex image reconstruction and computational processing.
Article
Biochemical Research Methods
Daniel Martin, J. Bocio-Nunez, Santiago F. Scagliusi, Pablo Perez, Gloria Huertas, Alberto Yufera, Merce Giner, Paula Daza
Summary: This study investigated the effects of direct current electrical stimulation on neuroblastoma and osteoblast cell lines and explored the potential applications of electrical stimulation in tissue engineering and regenerative medicine. The results showed that electrical stimulation promoted cell differentiation and proliferation, indicating its therapeutic potential for neural and bone tissue regeneration.
JOURNAL OF BIOLOGICAL ENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Elena Grao-Cruces, Alejandro Santos-Mejias, Ignacio Ortea, Elvira Marquez-Paradas, Maria E. Martin, Sergio Barrientos-Trigo, Beatriz Bermudez, Sergio Montserrat-de la Paz
Summary: This research aimed to study the protein composition of high-density lipoproteins (HDL) associated with postprandial hyperlipidemia. The results showed that compared to baseline, the expression of APOM, APOE, APOB, and APOA2 in HDL proteins was significantly altered in postprandial hyperlipidemia.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Computer Science, Information Systems
Santiago F. Scaliusi, Luis Gimenez, Pablo Perez, Daniel Martin, Alberto Olmo, Gloria Huertas, F. Javier Medrano, Alberto Yufera
Summary: Heart failure (HF) is a severe disease and a leading cause of death in society. Many HF patients are readmitted to the hospital after discharge due to lack of real-time supervision. This study proposes an electric model based on bioimpedance testing to assist with acute HF patient's supervision. The model calculates leg volume and can be measured using a wearable device. The results show that this method can effectively discriminate between healthy and unhealthy individuals.
Article
Chemistry, Analytical
Juan Alfonso Serrano, Pablo Perez, Paula Daza, Gloria Huertas, Alberto Yufera
Summary: Obtaining cell concentration measurements from a culture assay using bioimpedance is a useful method. This study aimed to find a method to obtain real-time cell concentration values by using an oscillator as the measurement circuit. Models were derived to estimate cell concentration in real time by using the oscillation frequency and amplitude. Real-time data of the cell concentration were obtained and compared to concentration data obtained by traditional optical methods.
Article
Engineering, Electrical & Electronic
Santiago F. Scagliusi, Luis Gimenez-Miranda, Pablo Perez-Garcia, Daniel Martin Fernandez, Francisco J. Medrano, Gloria Huertas, Alberto Yufera
Summary: Heart failure (HF) is a complex syndrome characterized by the inability of the heart to pump sufficient oxygenated and nutrient-rich blood to meet the body's needs. HF is the primary reason for hospitalization among individuals aged 65 and above, with a 50% mortality rate within five years of diagnosis. The lack of unanimous diagnostic criteria for HF has led to the proposal of bioimpedance (BI) analysis as a technique to detect changes in body volume due to edema. This research presents Volum, a portable device capable of real-time BI measurements in a cost-effective and non-invasive manner, aimed at improving patient monitoring at home for timely interventions.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Computer Science, Information Systems
Santiago Fernendez Scagliusi, Pablo Perez-Garcia, Andreea M. Oprescu, Daniel Martin Fernandez, Alberto Olmo, Gloria Huertas Sanchez, Alberto Yufera
Summary: This paper presents a low-power continuous patient monitoring method that improves clinical practice and patient outcomes by collecting biological impedance measurements. The method tracks the activity level and body states of patients, providing new insights into their status and assisting in remote monitoring of heart failure patients.
Proceedings Paper
Materials Science, Characterization & Testing
Santiago F. Scagliusi, Maggie Delano, Pablo Perez, Daniel Martin, Gloria Huertas, Alberto Olmo, Alberto Yufera
Summary: This study introduces Volum, a wearable device that measures calf bioimpedance to remotely monitor the progression of edema in patients with congestive heart failure. The device has shown high accuracy and potential for noninvasive monitoring of bioimpedance evolution. However, more research is needed to validate its measurements and explore its potential for improving patient outcomes.
2023 INTERNATIONAL WORKSHOP ON IMPEDANCE SPECTROSCOPY, IWIS
(2023)