dPCR: A Technology Review

Digital quantification of DNA via isothermal amplification on a self-driven microfluidic chip featuring hydrophilic film-coated polydimethylsiloxane

(2018) Yu-Dong Ma et al.   BIOSENSORS & BIOELECTRONICS

Centrifugal micro-channel array droplet generation for highly parallel digital PCR

(2017) Zitian Chen et al.   LAB ON A CHIP

Staggered trap arrays for robust microfluidic sample digitization

(2017) A. J. Sposito et al.   LAB ON A CHIP

Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples

(2017) Nathan G. Schoepp et al.   Science Translational Medicine

Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets: from variable nonsense to publication quality data

(2017) Sean C. Taylor et al.   Scientific Reports

Digital droplet LAMP as a microfluidic app on standard laboratory devices

(2016) Friedrich Schuler et al.   Analytical Methods

RT-qPCR and RT-Digital PCR: A Comparison of Different Platforms for the Evaluation of Residual Disease in Chronic Myeloid Leukemia

(2016) Mary Alikian et al.   CLINICAL CHEMISTRY

Comparison of Droplet Digital PCR and Quantitative PCR Assays for Quantitative Detection of Xanthomonas citri Subsp. citri

(2016) Yun Zhao et al.   PLoS One

A localized temporary negative pressure assisted microfluidic device for detecting keratin 19 in A549 lung carcinoma cells with digital PCR

(2015) Qingchang Tian et al.   Analytical Methods

Real-time PCR detection chemistry

(2015) E. Navarro et al.   CLINICA CHIMICA ACTA

Real-Time PCR and Droplet Digital PCR: two techniques for detection of theJAK2V617Fmutation in Philadelphia-negative chronic myeloproliferative neoplasms

(2015) G. Fontanelli et al.   International Journal of Laboratory Hematology

Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR: Clinical implications for quantification of Oseltamivir-resistant subpopulations

(2015) Sean C. Taylor et al.   JOURNAL OF VIROLOGICAL METHODS

Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection

(2015) Kaixiang Zhang et al.   LAB ON A CHIP

Centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet RPA

(2015) Friedrich Schuler et al.   LAB ON A CHIP

Microreactor Array Device

(2015) Peter Wiktor et al.   Scientific Reports

Comparison of four digital PCR platforms for accurate quantification of DNA copy number of a certified plasmid DNA reference material

(2015) Lianhua Dong et al.   Scientific Reports

Comparative Study of Sensitivity, Linearity, and Resistance to Inhibition of Digital and Nondigital Polymerase Chain Reaction and Loop Mediated Isothermal Amplification Assays for Quantification of Human Cytomegalovirus

(2014) Gavin Nixon et al.   ANALYTICAL CHEMISTRY

Self-Digitization Microfluidic Chip for Absolute Quantification of mRNA in Single Cells

(2014) Alison M. Thompson et al.   ANALYTICAL CHEMISTRY

One in a Million: Flow Cytometric Sorting of Single Cell-Lysate Assays in Monodisperse Picolitre Double Emulsion Droplets for Directed Evolution

(2014) Anastasia Zinchenko et al.   ANALYTICAL CHEMISTRY

Considerations for Digital PCR as an Accurate Molecular Diagnostic Tool

(2014) J. F. Huggett et al.   CLINICAL CHEMISTRY

Quantitative Cell-Free Circulating BRAFV600E Mutation Analysis by Use of Droplet Digital PCR in the Follow-up of Patients with Melanoma Being Treated with BRAF Inhibitors

(2014) M. F. Sanmamed et al.   CLINICAL CHEMISTRY

A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples

(2014) Ruth Hall Sedlak et al.   DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE

Critical appraisal of quantitative PCR results in colorectal cancer research: Can we rely on published qPCR results?

(2014) J.R. Dijkstra et al.   Molecular Oncology

Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples

(2014) Nejc Rački et al.   Plant Methods

Comparison of Droplet Digital PCR and Seminested Real-Time PCR for Quantification of Cell-Associated HIV-1 RNA

(2014) Maja Kiselinova et al.   PLoS One

Discriminating cellular heterogeneity using microwell-based RNA cytometry

(2014) Ivan K. Dimov et al.   Nature Communications

Rapid detection of single bacteria in unprocessed blood using Integrated Comprehensive Droplet Digital Detection

(2014) Dong-Ku Kang et al.   Nature Communications

Killer-cell Immunoglobulin-like Receptor gene linkage and copy number variation analysis by droplet digital PCR

(2014) Chrissy h Roberts et al.   Genome Medicine

Self-Digitization of Samples into a High-Density Microfluidic Bottom-Well Array

(2013) Thomas Schneider et al.   ANALYTICAL CHEMISTRY

Mechanistic Evaluation of the Pros and Cons of Digital RT-LAMP for HIV-1 Viral Load Quantification on a Microfluidic Device and Improved Efficiency via a Two-Step Digital Protocol

(2013) Bing Sun et al.   ANALYTICAL CHEMISTRY

Multiplexed Target Detection Using DNA-Binding Dye Chemistry in Droplet Digital PCR

(2013) Geoffrey P. McDermott et al.   ANALYTICAL CHEMISTRY

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

(2013) J. F. Huggett et al.   CLINICAL CHEMISTRY

Tolerance of Droplet-Digital PCR vs Real-Time Quantitative PCR to Inhibitory Substances

(2013) T. C. Dingle et al.   CLINICAL CHEMISTRY

Multiplex Picodroplet Digital PCR to Detect KRAS Mutations in Circulating DNA from the Plasma of Colorectal Cancer Patients

(2013) V. Taly et al.   CLINICAL CHEMISTRY

The need for transparency and good practices in the qPCR literature

(2013) Stephen A Bustin et al.   NATURE METHODS

Absolute quantification by droplet digital PCR versus analog real-time PCR

(2013) Christopher M Hindson et al.   NATURE METHODS

Methods for Applying Accurate Digital PCR Analysis on Low Copy DNA Samples

(2013) Alexandra S. Whale et al.   PLoS One

Highly Precise Measurement of HIV DNA by Droplet Digital PCR

(2013) Matthew C. Strain et al.   PLoS One

Droplet microfluidics driven by gradients of confinement

(2013) R. Dangla et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Single-molecule emulsion PCR in microfluidic droplets

(2012) Zhi Zhu et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Massively Parallel Single-Molecule and Single-Cell Emulsion Reverse Transcription Polymerase Chain Reaction Using Agarose Droplet Microfluidics

(2012) Huifa Zhang et al.   ANALYTICAL CHEMISTRY

Comparison of Droplet Digital PCR to Real-Time PCR for Quantitative Detection of Cytomegalovirus

(2012) R. T. Hayden et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Low-level detection and quantitation of cellular HIV-1 DNA and 2-LTR circles using droplet digital PCR

(2012) Timothy J. Henrich et al.   JOURNAL OF VIROLOGICAL METHODS

Digital LAMP in a sample self-digitization (SD) chip

(2012) Alexander Gansen et al.   LAB ON A CHIP

Self-priming compartmentalization digital LAMP for point-of-care

(2012) Qiangyuan Zhu et al.   LAB ON A CHIP

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation

(2012) Alexandra S. Whale et al.   NUCLEIC ACIDS RESEARCH

Molecular quantification of environmental DNA using microfluidics and digital PCR

(2012) Tatsuhiko Hoshino et al.   SYSTEMATIC AND APPLIED MICROBIOLOGY

Detecting biomarkers with microdroplet technology

(2012) Valérie Taly et al.   TRENDS IN MOLECULAR MEDICINE

Advances in multiplex PCR: balancing primer efficiencies and improving detection success

(2012) Daniela Sint et al.   Methods in Ecology and Evolution

Digital Isothermal Quantification of Nucleic Acids via Simultaneous Chemical Initiation of Recombinase Polymerase Amplification Reactions on SlipChip

(2011) Feng Shen et al.   ANALYTICAL CHEMISTRY

Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification

(2011) Leonardo B. Pinheiro et al.   ANALYTICAL CHEMISTRY

Evaluation of Digital PCR for Absolute DNA Quantification

(2011) Rebecca Sanders et al.   ANALYTICAL CHEMISTRY

Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR

(2011) Jason E. Kreutz et al.   ANALYTICAL CHEMISTRY

High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number

(2011) Benjamin J. Hindson et al.   ANALYTICAL CHEMISTRY

Quantitative polymerase chain reaction: a framework for improving the quality of results and estimating uncertainty of measurement

(2011) Kate R. Griffiths et al.   Analytical Methods

Multiplexed Quantification of Nucleic Acids with Large Dynamic Range Using Multivolume Digital RT-PCR on a Rotational SlipChip Tested with HIV and Hepatitis C Viral Load

(2011) Feng Shen et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Phaseguides: a paradigm shift in microfluidic priming and emptying

(2011) Paul Vulto et al.   LAB ON A CHIP

Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR

(2011) Qun Zhong et al.   LAB ON A CHIP

1-Million droplet array with wide-field fluorescence imaging for digital PCR

(2011) Andrew C. Hatch et al.   LAB ON A CHIP

Sensitive quantitation of minimal residual disease in chronic myeloid leukemia using nanofluidic digital polymerase chain reaction assay

(2011) Hyun-Gyung Goh et al.   LEUKEMIA & LYMPHOMA

Megapixel digital PCR

(2011) Kevin A Heyries et al.   NATURE METHODS

Effect of sustained elevated temperature prior to amplification on template copy number estimation using digital polymerase chain reaction

(2010) Somanath Bhat et al.   ANALYST

Self-Digitization of Sample Volumes

(2010) Dawn E. Cohen et al.   ANALYTICAL CHEMISTRY

Spinning Disk Platform for Microfluidic Digital Polymerase Chain Reaction

(2010) Scott O. Sundberg et al.   ANALYTICAL CHEMISTRY

Ultrasensitive Detection of microRNAs by Exponential Isothermal Amplification

(2010) Hongxia Jia et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Digital PCR on a SlipChip

(2010) Feng Shen et al.   LAB ON A CHIP

Digital MDA for enumeration of total nucleic acid contamination

(2010) P. C. Blainey et al.   NUCLEIC ACIDS RESEARCH

Single molecule detection in nanofluidic digital array enables accurate measurement of DNA copy number

(2009) Somanath Bhat et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Controlling the Retention of Small Molecules in Emulsion Microdroplets for Use in Cell-Based Assays

(2009) Fabienne Courtois et al.   ANALYTICAL CHEMISTRY

Droplet-Based Microfluidic Systems for High-Throughput Single DNA Molecule Isothermal Amplification and Analysis

(2009) Linas Mazutis et al.   ANALYTICAL CHEMISTRY

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

SlipChip

(2009) Wenbin Du et al.   LAB ON A CHIP

On-Chip Single-Copy Real-Time Reverse-Transcription PCR in Isolated Picoliter Droplets

(2008) N. Reginald Beer et al.   ANALYTICAL CHEMISTRY

High-Throughput Single Copy DNA Amplification and Cell Analysis in Engineered Nanoliter Droplets

(2008) Palani Kumaresan et al.   ANALYTICAL CHEMISTRY

High-Throughput Quantitative Polymerase Chain Reaction in Picoliter Droplets

(2008) Margaret Macris Kiss et al.   ANALYTICAL CHEMISTRY

Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets

(2008) Yolanda Schaerli et al.   ANALYTICAL CHEMISTRY

Biocompatible surfactants for water-in-fluorocarbon emulsions

(2008) C. Holtze et al.   LAB ON A CHIP

Simple, robust storage of drops and fluids in a microfluidic device

(2008) Hakim Boukellal et al.   LAB ON A CHIP

Mathematical Analysis of Copy Number Variation in a DNA Sample Using Digital PCR on a Nanofluidic Device

(2008) Simant Dube et al.   PLoS One