Review
Cell Biology
Luise Mueller, Antje Tunger, Manja Wobus, Malte von Bonin, Russell Towers, Martin Bornhaeuser, Francesco Dazzi, Rebekka Wehner, Marc Schmitz
Summary: Mesenchymal stromal cells (MSCs) have the ability to regulate and inhibit the function of immune cells, playing a crucial role in the pathogenesis of inflammatory disorders and showing promise in the treatment of conditions such as acute graft-versus-host disease, graft rejection, and autoimmune diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Medicine, General & Internal
Suncica Kapor, Juan F. Santibanez
Summary: This review discusses the main features of MDSCs and MSCs in myeloid malignancies, revealing that MDSCs are elevated in numbers and exhibit strong immunosuppressive capabilities, while MSCs not only have immunosuppressive properties but also regulate the growth, apoptosis, and chemotherapy resistance of myeloid leukemia cells.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Oncology
Lilian van Vlerken-Ysla, Yulia Y. Tyurina, Valerian E. Kagan, Dmitry I. Gabrilovich
Summary: This review discusses the classification of myeloid cells using single-cell omics technologies in the tumor microenvironment (TME). It suggests that the biology of myeloid cells is primarily determined by a limited number of functional states, including classical and pathological activation states. The article also explores the relationship between lipid peroxidation of myeloid cells and their activation state in the TME, highlighting its potential as a therapeutic target.
Article
Biochemistry & Molecular Biology
Alexey Yu. Lupatov, Roza Yu. Saryglar, Valentina V. Vtorushina, Rimma A. Poltavtseva, Oxana A. Bystrykh, Vladimir D. Chuprynin, Lyubov V. Krechetova, Stanislav V. Pavlovich, Konstantin N. Yarygin, Gennady T. Sukhikh
Summary: A comparative analysis was conducted on the cell surface markers and immunological properties of cell cultures from normal endometrium and endometrioid heterotopias in women with extragenital endometriosis. Despite similar phenotypes, stromal cells from endometrioid heterotopias showed enhanced pro-inflammatory and immunosuppressive activities compared to those from eutopic endometrium. The results suggest that these differences may impact the pathogenesis and progression of the disease.
Article
Pharmacology & Pharmacy
Paula Laranjeira, Monia Pedrosa, Catia Duarte, Susana Pedreiro, Brigida Antunes, Tania Ribeiro, Francisco dos Santos, Antonio Martinho, Margarida Fardilha, M. Rosario Domingues, Manuel Abecasis, Jose Antonio Pereira da Silva, Artur Paiva
Summary: This study found that BM-MSC effectively reduced TNF-alpha, CD83, CCR7, and MIP-1 beta protein levels in mDCs and all monocyte subsets in RA patients. BM-MSC exhibited a suppressive action over antigen-presenting cells from RA patients, potentially affecting their ability to stimulate the immune adaptive response at different levels.
Review
Immunology
Ya-Jui Lin, Caren Yu-Ju Wu, Janet Yuling Wu, Michael Lim
Summary: Glioblastoma is a highly aggressive glioma with resistance to immunotherapy, primarily due to the unique immune environment. Myeloid cells play a significant role in the glioma microenvironment and reprogramming them has emerged as a revolutionary immunotherapy for glioma treatment. This article provides a detailed classification and analysis of myeloid cells in glioma TME, aiming to offer new insights and therapeutic approaches for improving treatment efficacy in glioma patients.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Kevin J. Paavola, Julie M. Roda, Vicky Y. Lin, Peirong Chen, Kyle P. O'Hollaren, Richard Ventura, Suzanne C. Crawley, Betty Li, Hung-I H. Chen, Seth Malmersjo, Nikolai A. Sharkov, Geoffrey Horner, Wei Guo, Alan K. Kutach, Kalyani Mondal, Zhen Zhang, Joshua S. Lichtman, Christina Song, Lee B. Rivera, Wenhui Liu, Jian Luo, Yan Wang, Mark J. Solloway, Bernard B. Allan, Avantika Kekatpure, Shelley R. Starck, Raj Haldankar, Bin Fan, Chun Chu, Jie Tang, Martina Molgora, Marco Colonna, Daniel D. Kaplan, Jer-Yuan Hsu
Summary: This study revealed that fibronectin interacting with ILT3 plays a key role in polarizing myeloid cells towards a suppressive phenotype. Blocking this interaction can reprogram tumor-associated myeloid cells towards a stimulatory phenotype and potentially enhance antitumor T-cell responses.
CANCER IMMUNOLOGY RESEARCH
(2021)
Article
Immunology
Haiping He, Atsuko Takahashi, Takeo Mukai, Akiko Hori, Miwako Narita, Arinobu Tojo, Tonghua Yang, Tokiko Nagamura-Inoue
Summary: Mesenchymal stromal cells (MSCs) were primed with Triptolide (TPL) to enhance their immunosuppressive abilities. TPL-primed UC-MSCs showed stronger anti-proliferative effects on activated T cells, potentially offering a novel immunosuppressive cell therapy. The upregulation of IDO-1 and induction of PD-L1 in TPL-primed UC-MSCs enhanced their immunosuppressive potency.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Lauren E. Hillers-Ziemer, Abbey E. Williams, Amanda Janquart, Caitlin Grogan, Victoria Thompson, Adriana Sanchez, Lisa M. Arendt
Summary: Obesity promotes rapid growth and metastases of mammary tumors in mice by increasing recruitment of myeloid lineage cells and deposition of collagen fibers within the lungs. Lung stromal cells isolated from obese mice show higher expression of extracellular matrix proteins and inflammatory cytokines, facilitating metastatic growth prior to tumor formation. Understanding these mechanisms may provide new therapeutic options for obese breast cancer patients.
Review
Cell Biology
Rodrigo Nalio Ramos, Samuel Campanelli Freitas Couto, Theo Gremen M. Oliveira, Paulo Klinger, Tarcio Teodoro Braga, Eduardo Magalhaes Rego, Jose Alexandre M. Barbuto, Vanderson Rocha
Summary: CAR engineering for T cells and NK cells shows promising results in hematologic cancers but has not proven effective for solid tumors. The scientific community is now looking into mononuclear phagocytes as potential candidates for CAR expression, due to their abilities to overcome barriers posed by solid tumors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jingshan Cai, Yudan Cui, Jun Yang, Shengjun Wang
Summary: MDSCs play a crucial role in tumor immunity, closely linked to tumor EMT and possessing unique functions in an immunosuppressive environment.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Article
Cell Biology
Jean-Pierre Bikorimana, Wael Saad, Jamilah Abusarah, Malak Lahrichi, Sebastien Talbot, Riam Shammaa, Moutih Rafei
Summary: This study demonstrates the suppressive properties of MSCs expressing high levels of CD146 and suggests the involvement of CD146-mediated efferocytosis in enhancing the therapeutic effects of MSCs.
Article
Biochemistry & Molecular Biology
Alvin Wen Choong Chua, Dianyang Guo, Jia Chi Tan, Frances Ting Wei Lim, Chee Tian Ong, Jeyakumar Masilamani, Tony Kiat Hon Lim, William Ying Khee Hwang, Ivor Jiun Lim, Jinmiao Chen, Toan Thang Phan, Xiubo Fan
Summary: This study investigated the therapeutic efficacy and working mechanisms of human umbilical cord lining mesenchymal stromal cells (CL-MSCs) in lupus-prone mice. The results showed that CL-MSCs improved the survival and kidney function of mice by reducing lupus nephritis activity and chronicity, and lymphocyte infiltration. Additionally, CL-MSCs reduced urinary albumin-to-creatinine ratio and the production of anti-dsDNA and isotype antibodies. Mechanistically, CL-MSCs suppressed splenic neutrophils and monocytes/macrophages, reduced proinflammatory cytokines, and modulated immunomodulatory pathways. These findings highlight the potential of CL-MSCs as a novel therapy for systemic lupus erythematosus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Candice Bohaud, Rafael Contreras-Lopez, Jholy de la Cruz, Claudia Terraza-Aguirre, Mingxing Wei, Farida Djouad, Christian Jorgensen
Summary: Osteoarthritis is a multifaceted degenerative and inflammatory joint disorder, with current therapies often only providing short-term benefits without complete structural and functional reconstitution. Identifying novel therapeutic targets and strategies is crucial to achieve long-term clinical benefits for OA patients.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Inaya Hayek, Valentin Schatz, Christian Bogdan, Jonathan Jantsch, Anja Luehrmann
Summary: The tissue microenvironment, including factors like oxygen concentration, plays a crucial role in host-pathogen interactions. Hypoxia and hypoxia-inducible factors (HIF) have been identified as important factors affecting both pathogens and hosts, influencing inflammatory and antimicrobial activities in myeloid cells, bacterial responses to low oxygen conditions, and the metabolism of immune cells. Additionally, the consequences of hypoxia and HIF alpha activation on various invading pathogens like Pseudomonas aeruginosa, Mycobacterium tuberculosis, Coxiella burnetii, Salmonella enterica, and Staphylococcus aureus, including how these pathogens sense and respond to hypoxic environments, are discussed.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Letter
Hematology
Alice Pievani, Samantha Donsante, Chiara Tomasoni, Alessandro Corsi, Francesco Dazzi, Andrea Biondi, Mara Riminucci, Marta Serafini
Editorial Material
Surgery
Alberto Sanchez-Fueyo, Francesco Dazzi
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Review
Cell Biology
Luise Mueller, Antje Tunger, Manja Wobus, Malte von Bonin, Russell Towers, Martin Bornhaeuser, Francesco Dazzi, Rebekka Wehner, Marc Schmitz
Summary: Mesenchymal stromal cells (MSCs) have the ability to regulate and inhibit the function of immune cells, playing a crucial role in the pathogenesis of inflammatory disorders and showing promise in the treatment of conditions such as acute graft-versus-host disease, graft rejection, and autoimmune diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Vipul Sheth, Victoria Potter, Hugues de Lavallade, Shreyans Gandhi, Austin Kulasekararaj, Pramila Krishnamurthy, Varun Mehra, Francesco Dazzi, Ghulam Mufti, Antonio Pagliuca, Donal Mclornan, Kavita Raj
Summary: The study found that pre-emptive DLI is an effective strategy in lowering the risk of relapse in patients with hematological malignancies. Patients who received DLI treatment had significantly improved overall survival, while those who did not receive treatment had poor outcomes.
BLOOD CANCER JOURNAL
(2021)
Review
Cell Biology
Ester Pfeifer, Joy M. Burchell, Francesco Dazzi, Debashis Sarker, Richard Beatson
Summary: PDAC is associated with poor prognosis due to advanced stage at diagnosis and aggressive tumor biology. Efforts to target CAF, a predominant cell type in the tumor microenvironment, have shown disappointing results in clinical trials.
Article
Multidisciplinary Sciences
James W. Opzoomer, Joanne E. Anstee, Isaac Dean, Emily J. Hill, Ihssane Bouybayoune, Jonathan Caron, Tamara Muliaditan, Peter Gordon, Dominika Sosnowska, Rosamond Nuamah, Sarah E. Pinder, Tony Ng, Francesco Dazzi, Shahram Kordasti, David R. Withers, Toby Lawrence, James N. Arnold
Summary: In this study, it was found that TAMs expressing Lyve-1 play a role in promoting tumor growth and maintaining a population of perivascular mesenchymal cells. Through controlling the expansion of the perivascular mesenchymal population, Lyve-1(+) TAMs create a proangiogenic niche in the tumor microenvironment.
Article
Cell Biology
Marco Romano, Raul Elgueta, Daniel McCluskey, Ana Maria Ortega-Prieto, Emilie Stolarczyk, Francesco Dazzi, Baltasar Lucendo-Villarin, Jose Meseguer-Ripolles, James Williams, Giorgia Fanelli, David C. Hay, Fiona M. Watt, Giovanna Lombardi
Summary: Regenerative medicine aims to replace damaged tissues by stimulating tissue repair or transplanting cells. Pluripotent stem cells have the potential for unlimited cell production, making them valuable for regenerative medicine. However, immune responses pose a challenge to success. This study investigates the immunological properties of human pluripotent stem cell-derived hepatocyte-like cells and shows their potential for in vitro immunoregulation.
Article
Biophysics
Alice Pievani, Valentina Granata, Giacomo Desantis, Laura Antolini, Sara Ornaghi, Antonio Galleu, Andrea Biondi, Bernhard Gentner, Francesco Dazzi, Marta Serafini
Summary: Monocytes are effective at improving engraftment in HSPC transplantation, particularly in cases of limited cell doses such as CB transplantation and HSC gene therapy. Co-culturing monocytes increases survival and stemness of CB-CD34(+) cells. Soluble factors and direct-cell contact interactions play critical roles in the HSC-monocyte crosstalk.
BONE MARROW TRANSPLANTATION
(2022)
Article
Biotechnology & Applied Microbiology
Sandhya Moise, Luigi Dolcetti, Francesco Dazzi, Paul Roach, Lee Buttery, Sheila MacNeil, Nick Medcalf
Summary: This study evaluated the immunosuppressive effect of alginate-encapsulated MSCs on proliferating mouse splenocytes. The results showed that MSCs significantly suppressed splenocyte proliferation via both direct and indirect mechanisms. Additionally, the study demonstrated for the first time that MSCs can sustain immunosuppressive activity when physically isolated from splenocytes.
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2022)
Article
Oncology
Karol Nowicki-Osuch, Lizhe Zhuang, Tik Shing Cheung, Emily L. Black, Neus Masque-Soler, Ginny Devonshire, Aisling M. Redmond, Adam Freeman, Massimilliano di Pietro, Nastazja Pilonis, Wladyslaw Januszewicz, Maria O'Donovan, Simon Tavare, Jacqueline D. Shields, Rebecca C. Fitzgerald
Summary: Intestinal metaplasia in the esophagus (BE-IM) and stomach (GIM) are precursors for esophageal and gastric adenocarcinoma. This study constructs a single-cell RNA-sequencing atlas of the gastrointestinal tract, showing that BE-IM and GIM share molecular features and exhibit phenotypic mosaicism. Additionally, the study reveals that E-GM resembles atrophic gastritis and has a lower mutational burden than BE-IM. The findings suggest that BE-IM and GIM can be considered similar entities, enabling shared detection and treatment strategies.
Article
Cell Biology
Joanne E. Anstee, Karen T. Feehan, James W. Opzoomer, Isaac Dean, Henrike P. Muller, Meriem Bahri, Tik Shing Cheung, Kifayathullah Liakath-Ali, Ziyan Liu, Desmond Choy, Jonathan Caron, Dominika Sosnowska, Richard Beatson, Tamara Muliaditan, Zhengwen An, Cheryl E. Gillett, Guocheng Lan, Xiangang Zou, Fiona M. Watt, Tony Ng, Joy M. Burchell, Shahram Kordasti, David R. Withers, Toby Lawrence, James N. Arnold
Summary: This study describes a population of LYVE-1-expressing TAMs that form complex multi-cellular nest structures and are distributed around blood vessels in breast cancer tumors. The researchers found that LYVE-1+ TAMs develop in response to IL-6, and their nest formation is guided by a CCR5-dependent signaling axis. Blocking the development of LYVE-1+ TAMs or their nest structures enhances the recruitment of CD8+ T cells to the tumor and improves response to chemotherapy.
DEVELOPMENTAL CELL
(2023)
Review
Immunology
Chiara Giacomini, Cecilia Graneli, Ryan Hicks, Francesco Dazzi
Summary: Mesenchymal stromal cells (MSCs) have shown good safety but mixed efficacy in the treatment of various clinical conditions. The heterogeneity of cell preparations, lack of mechanistic understanding, and poorly defined criteria for patient stratification have hindered informative clinical trials. However, studies have shown that apoptotic MSCs can be phagocytosed by anti-inflammatory cells, suggesting that the activation state of cytotoxic T or NK cells can be used as a biomarker for predicting clinical responses to MSC treatment. MSCs can orchestrate inflammatory responses through different mechanisms, including cell-contact and inflammatory cytokine-mediated licensing.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Meeting Abstract
Surgery
Caterina Lonati, Carlotta Lista, Michele Battistin, Alessandra Bertacco, Daniele Dondossola, Margherita Carbonaro, Umberto Cillo, Francesco Dazzi
TRANSPLANT INTERNATIONAL
(2021)
Meeting Abstract
Oncology
James W. Opzoomer, Joanne E. Anstee, Isaac Dean, Emily J. Hill, Ihssane Bouybayoune, Jonathan Caron, Tamara Muliaditan, Peter Gordon, Rosamond Nuamah, Sarah E. Pinder, Tony Ng, Francesco Dazzi, Shahram Kordasti, David R. Withers, Toby Lawrence, James N. Arnold
Meeting Abstract
Biophysics
Vipul Sheth, Victoria Potter, Donal McLornan, Hugues Delavallade, Shreyans Gandhi, Austin Kulasekararaj, Francesco Dazzi, Judith Marsh, Ghulam Mufti, Antonio Pagliuca, Kavita Raj
BONE MARROW TRANSPLANTATION
(2020)