Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 612, Issue -, Pages 788-798Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.scitotenv.2017.08.295
Keywords
Anaerobic digestion; Sewage sludge; Antibiotic resistance gene; Thermophilic digester; Microbial community
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Funding
- National Natural Science Foundation of China (NSFC) [51378189, 51578223, 51521006]
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Spread of antibiotic resistance genes (ARGs) originating from sewage sludge is highlighted as an eminent health threat. This study established a thermophilic anaerobic digester using one-step startup strategy to quickly remove tetracycline and sulfonamides resistance genes from sewage sludge. At least 20 days were saved in the startup period from mesophilic to thermophilic condition. Based on the results of 16S rDNA amplicons sequencing and predicted metagenomic method, the successful startup largely relied on the fast colonization of core thermophilic microbial population (e.g. Firmicutes, Proteobacteria, Actinobacteria). Microbial metabolic gene pathways for substrate degradation and methane production was also increased by one-step mode. In addition, real-time quantitative PCR approach revealed that most targeted tetracycline and sulfonamides resistance genes ARGs (sulI, tetA, tetO, tetX) were substantially removed during thermophilic digestion (removal efficiency > 80%). Network analysis showed that the elimination of ARGs was attributed to the decline of their horizontal (intI1 item) and vertical (potential hosts) transfer-related elements under high-temperature. This research demonstrated that rapid startup thermophilic anaerobic digestion of waste water solids would be a suitable technology for reducing quantities of various ARGs. (C) 2017 Elsevier B.V. All rights reserved.
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