Journal
SCIENCE OF THE TOTAL ENVIRONMENT
Volume 636, Issue -, Pages 1355-1361Publisher
ELSEVIER
DOI: 10.1016/j.scitotenv.2018.04.229
Keywords
CadR; Chromosomal surface display; Pseudomonas aeruginosa; Cadmium; Bioremediation
Categories
Funding
- National Natural Science Foundation of China [51521006, 51679084, 51378190, 51579096, 51508177, 51709101]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT-13R17]
Ask authors/readers for more resources
Genetically engineered bacteria for pollution control of heavy metal have been widely studied, however, using Pseudomonas aeruginosa (P. aeruginosa) that can adapt to various circumstances to remediate heavy metal pollution is rarely reported. In this study, we employed CadR, a cadmium (Cd)-specific binding protein, displaying on the surface of P. aeruginosa with chromosomal expression. The genetically engineered (GE) P. aeruginosa still flourished in the 30th generation in the LB broth which contained 100 mu M Cd(II), exhibiting an excellent genetic stability. Chromosomally expressed P. aeruginosa showed an adsorption capacity of up to 131.9 mu mol/g of Cd(II). In addition, the low concentration of the coexisting two valence ions has no significant effect on adsorption capacity of Cd(II). This study provides a direction for application of P. aeruginosa in environment remediation. (C) 2018 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available