Article
Biochemistry & Molecular Biology
Yu Wei, Juan Du, Zhangwu Zhao
Summary: Sleep is crucial for animal health and survival, and the study found that the 14-3-3 epsilon protein regulates Drosophila sleep by activating specific genes, with involvement of dopamine and octopamine receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
William P. Pederson, Jaime M. Cyphert-Daly, Robert M. Tighe, Loretta G. Que, Julie G. Ledford
Summary: The study suggests that expression of a glutamine (Q) at position 223 in SP-A2 is more protective than lysine (K) in acute exposures to ozone. SP-A2 223Q variant mice showed lower levels of inflammation markers post-O3 exposure, indicating a reduced impact on respiratory diseases.
Article
Pharmacology & Pharmacy
Claire C. Munier, Christian Ottmann, Matthew W. D. Perry
Summary: The 14-3-3 proteins play crucial roles in regulating the inflammatory response at genetic, molecular, and cellular levels. They affect key components of the immune response and can lead to clinical syndromes when their recognition processes are disrupted. Abnormal levels of 14-3-3 contribute to undesirable immune responses and chronic inflammatory conditions.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Bente A. Somsen, Fenna W. B. Craenmehr, Wei-Hong W. Liu, Auke A. Koops, Marloes A. M. Pennings, Emira J. Visser, Christian Ottmann, Peter J. Cossar, Luc Brunsveld
Summary: Molecular glues represent a novel approach in drug discovery, however, the targeted stabilization of protein complexes is still challenging due to a lack of drug design rules. This study demonstrates the successful development of a peptide-based molecular glue that selectively stabilizes the 14-3-3/ChREBP protein-protein interaction by utilizing the functional mapping of hotspots.
Article
Oncology
Manlan Guo, Minyi He, Yi Zhang, Weiwen Liu, Min Qi, Zhifeng Liu, Guozhong Yi, Shengze Deng, Yaomin Li, Xuegang Sun, Liang Zhao, Tengxiang Chen, Yawei Liu
Summary: The translocation of 14-3-3 protein epsilon (14-3-3e) is involved in Triptolide (Tp)-induced inhibition of colorectal cancer (CRC) cell proliferation. 14-3-3e translocation induces cell death and mediates the nucleo-cytoplasmic shuttling of heterogeneous nuclear ribonucleoproteins C1/C2 (hnRNP C), leading to proliferation inhibition, cell cycle arrest, and autophagic processes in CRC cells treated with Tp.
CANCER BIOLOGY & THERAPY
(2023)
Article
Microbiology
Qiufan Xu, Jianpeng Liang, Jiaqi Jin, Wanyan Wu, Jinlian Ren, Jiayu Ruan, Lei Fan, Weifeng Yuan, Juncheng Cai, Qiuyan Lin, Bin Xiang, Chan Ding, Tao Ren, Libin Chen
Summary: This study investigates the relationship between 14-3-3 epsilon and Newcastle disease virus (NDV) infection, revealing that NDV infection leads to the degradation of 14-3-3 epsilon and that 14-3-3 epsilon inhibits the replication of NDV.
VETERINARY MICROBIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Veronika Obsilova, Tomas Obsil
Summary: Signal transduction cascades efficiently transmit chemical and/or physical signals from the extracellular environment to intracellular compartments, thereby eliciting an appropriate cellular response. 14-3-3 proteins, as a family of highly conserved scaffolding molecules, play a crucial role in modulating the function of other proteins primarily through phosphorylation-dependent mechanisms. They participate in key cellular processes such as cell-cycle control, apoptosis, signal transduction, energy metabolism, and protein trafficking. However, our understanding of the molecular mechanisms by which 14-3-3 proteins regulate their binding partners remains insufficient, despite intensive research into their protein-protein interactions. This review article aims to provide an overview of recent structural studies of 14-3-3 protein complexes in order to further explore the regulatory mechanisms of these proteins.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Plant Sciences
Liyun Yang, Jing Gao, Mengze Gao, Lingyan Jiang, Lijuan Luo
Summary: Plant plasma membranes (PM) play a crucial role in maintaining intracellular stability and exchanging information with the external environment. This study developed a simplified method to enrich PM proteins in Stylosanthes plants, which can be used to study the dynamics of PM proteome in response to various stresses.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Immunology
Dave Francisco, Ying Wang, Craig Marshall, Michelle Conway, Kenneth J. Addison, Dean Billheimer, Hiroki Kimura, Mari Numata, Hong W. Chu, Dennis R. Voelker, Monica Kraft, Julie G. Ledford
Summary: Surfactant Protein-A (SP-A) is an innate immune modulator that regulates various pulmonary host defense functions. Dysfunctional SP-A is found in asthma, which may be linked to genetic heterogeneity. SP-A 223Q offers better protection against inflammation and mucin production compared to SP-A 223K, and individuals with two copies of the major 223Q allele experience improved lung function and asthma control. Short peptides of SP-A2 retain anti-inflammatory properties similar to the endogenous protein.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Kun Fan, Kaihua Zhu, Jiwen Wang, Xiaojian Ni, Sheng Shen, Zijun Gong, Xi Cheng, Cheng Zhang, Han Liu, Tao Suo, Xiaoling Ni, Houbao Liu
Summary: This study identified the K50 acetylation of 14-3-3 epsilon protein and investigated its roles and mechanism in cholangiocarcinoma progression. The acetylation of K50 regulated the activation of YAP1 in cholangiocarcinoma by inhibiting the combination of 14-3-3 epsilon with phosphorylated YAP1. The findings provide potential targets for cholangiocarcinoma therapy.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Cell Biology
Jia-Hua Qu, Kirill Tarasov, Khalid Chakir, Yelena S. Tarasova, Daniel R. Riordon, Edward G. Lakatta
Summary: This study investigated the structure and function of the cardiac 14-3-3 protein interactome, revealing that the proteins bound to 14-3-3 are mainly related to mitochondria and play a crucial role in regulating cardiac metabolism and proteostasis.
Article
Plant Sciences
Xiaohui Li, Peipei Zhu, Yen-Ju Chen, Lei Huang, Diwen Wang, David T. Newton, Chuan-Chih Hsu, Guang Lin, W. Andy Tao, Christopher J. J. Staiger, Chunhua Zhang
Summary: To sustain normal growth and responses to environmental cues, plants regulate the composition of plasma membrane proteins through mechanisms such as delivery, stability, and internalization. The exocyst complex, a conserved cellular process in eukaryotes, contributes to exocytosis by tethering secretory vesicles and is also involved in membrane recycling and autophagy. By using a small molecule inhibitor and quantitative proteomic analysis, the study found that the exocyst complex mediates the transport of specific subsets of plasma membrane proteins involved in various cellular functions. Live-cell imaging also confirmed the effect of the inhibitor on the spatial distribution of exocyst complex subunits.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Heidi Pertl-Obermeyer, Ana Gimeno, Verena Kuchler, Evrim Servili, Shuai Huang, Han Fang, Veronika Lang, Katharina Sydow, Magdalena Poeckl, Waltraud X. Schulze, Gerhard Obermeyer
Summary: Pollen tube growth and fertilization are precisely regulated processes involving cellular and molecular events, such as the activity and phosphorylation regulation of the plasma membrane H+ ATPase. Phosphorylation and pH modulation significantly influence the activity of H+ ATPase, while the metabolic components have minimal effects. Local regulation of H+ ATPase activity generates localized H+ influxes and effluxes, as well as pH gradients in the pollen tube tip, instead of heterogeneous localized distribution in the plasma membrane.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Wei Jiang, Jing He, Mohammad Babla, Ting Wu, Tao Tong, Adeel Riaz, Fanrong Zeng, Yuan Qin, Guang Chen, Fenglin Deng, Zhong-Hua Chen
Summary: This review comprehensively explores the roles of 14-3-3 proteins and H+-ATPases in plant cell signaling and their potential in enhancing plant stress tolerance. The molecular evolution of these proteins in green plants is analyzed, and their stress-specific expression in different plant species is discussed.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Oncology
Markus Winter, Matjaz Rokavec, Heiko Hermeking
Summary: Downregulation of 14-3-3 sigma in colorectal cancer is associated with metastasis and poor survival of patients, and its inactivation in a murine tumor model drives intestinal tumor formation and epithelial-mesenchymal transition.
Article
Respiratory System
Raphael Borie, Caroline Kannengiesser, Katerina Antoniou, Francesco Bonella, Bruno Crestani, Aurelie Fabre, Antoine Froidure, Liam Galvin, Matthias Griese, Jan C. Grutters, Maria Molina-Molina, Venerino Poletti, Antje Prasse, Elisabetta Renzoni, Jasper van der Smagt, Coline H. M. van Moorsel
Summary: The discovery of gene mutations that cause pulmonary fibrosis confirms the genetic predisposition to this condition. While genetic sequencing is already part of routine clinical practice for familial pulmonary fibrosis cases in some countries, it has not been widely incorporated into interstitial lung disease services and lacks international consensus.
EUROPEAN RESPIRATORY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Lorenza Vantaggiato, Enxhi Shaba, Paolo Cameli, Laura Bergantini, Miriana d'Alessandro, Alfonso Carleo, Giusy Montuori, Luca Bini, Elena Bargagli, Claudia Landi
Summary: Idiopathic pulmonary fibrosis (IPF) is a chronic and irreversible fibrosing interstitial pneumonia of unknown cause. IPF increases the risk of lung cancer (LC), and the two diseases share similar risk factors and pathogenic pathways. A proteomic study on bronchoalveolar lavage fluid (BALF) samples identified dysregulated proteins related to inflammation, immune response, lipid metabolism, and cell adhesion in LC associated with IPF (LC-IPF) patients. These potential biomarkers could potentially improve the management of LC-IPF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Allergy
Bas Smits, Sigune Goldacker, Suranjith Seneviratne, Marion Malphettes, Hilary Longhurst, Omar E. Mohamed, Carla Witt-Rautenberg, Lucy Leeman, Eva Schwaneck, Isabelle Raymond, Kilifa Meghit, Annette Uhlmann, Christine Winterhalter, Joris van Montfrans, Marion Klima, Sarita Workman, Claire Fieschi, Lorena Lorenzo, Sonja Boyle, Shamin Onyango-Odera, Suzanne Price, Marc Schmalzing, Valerie Aurillac, Antje Prasse, Ieneke Hartmann, Jennifer J. Meerburg, Mariette Kemner-van de Corput, Harm Tiddens, Bodo Grimbacher, Peter Kelleher, Smita Y. Patel, Anne-Sophie Korganow, Jean-Francois Viallard, Hans-Peter Tony, Claire Bethune, Hendrik Schulze-Koops, Torsten Witte, Aarnoud Huissoon, Helen Baxendale, Sofia Grigoriadou, Eric Oksenhendler, Siobhan O. Burns, Klaus Warnatz
Summary: This study analyzed the effect of high-dose corticosteroids on granulomatous and lymphocytic interstitial lung disease (gl-ILD) using high-resolution computed tomography (HRCT) scans and pulmonary function tests (PFT). The results showed that high-dose corticosteroid treatment significantly improved HRCT scores and lung function, achieving long-term remission in 42% of patients with gl-ILD. However, low-dose maintenance therapy was not beneficial and had poor efficacy in relapsing disease.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Denise Bonente, Laura Bianchi, Rossana De Salvo, Claudio Nicoletti, Elena De Benedetto, Tommaso Bacci, Luca Bini, Giovanni Inzalaco, Lorenzo Franci, Mario Chiariello, Gian Marco Tosi, Eugenio Bertelli, Virginia Barone
Summary: Epiretinal membranes (ERMs) are pathological tissues that form in the vitreoretinal interface, leading to vision loss. Understanding the molecular dysfunctions that contribute to ERM development is crucial. Our bioinformatics study identified CD44 as a central regulator of ERM dynamics, and its interaction with PDPN could promote migration in epithelial cells. PDPN, overexpressed in various cancers, plays a relevant role in fibrotic and inflammatory pathologies, modulating signaling pathways involved in ERM formation. Understanding the role of PDPN may open new therapeutic options for fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Toby M. Maher, Paul Ford, Kevin K. Brown, Ulrich Costabel, Vincent Cottin, Sonye K. Danoff, Irene Groenveld, Eric Helmer, R. Gisli Jenkins, Julie Milner, Geert Molenberghs, Bjorn Penninckx, Matthew J. Randall, Bernt Van den Blink, Ann Fieuw, Charlotte Vandenrijn, Sanda Rocak, Ineke Seghers, Lixin Shao, Amit Taneja, Garrit Jentsch, Timothy R. Watkins, Wim A. Wuyts, Michael Kreuter, Nadia Verbruggen, Niyati Prasad, Marlies S. Wijsenbeek
Summary: This study aimed to assess the efficacy and safety of the autotaxin inhibitor ziritaxestat in patients with idiopathic pulmonary fibrosis (IPF). The study found that ziritaxestat did not improve clinical outcomes compared with placebo in patients with IPF.
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
(2023)
Article
Rheumatology
Martin Aringer, Dirk Koschel, Thomas Doerner, Philipp Sewerin, Antje Prasse, Torsten Witte
Summary: Sjogren's syndrome is a potential autoimmune cause of interstitial lung disease. The diagnostic pathway for this syndrome is not well-defined compared to other autoimmune diseases. Subjective sicca symptoms and specific autoantibodies have limitations in their sensitivity and/or specificity as screening tests. To address this, a consensus has been developed to include additional antibodies and objective measures in the diagnosis.
ZEITSCHRIFT FUR RHEUMATOLOGIE
(2023)
Article
Respiratory System
Juergen Behr, Francesco Bonella, Andreas Guenther, Dirk Koschel, Antje Prasse, David Pittrow, Jens Klotsche, Michael Kreuter
Summary: This study is a long-term registry aimed to describe the characteristics, management, and course of newly diagnosed patients with fibrosing interstitial lung disease (fILD) in Germany. So far, 360 patients have been enrolled, and a total of 900 patients are planned to be included over three years. The registry will provide important data on patient characteristics, management, and trajectories, and allow for comparisons with other countries.
BMC PULMONARY MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Tommaso Pianigiani, Lorenzo Alderighi, Martina Meocci, Maddalena Messina, Beatrice Perea, Simona Luzzi, Laura Bergantini, Miriana D'Alessandro, Rosa Metella Refini, Elena Bargagli, Paolo Cameli
Summary: Through a systematic review of published articles, it was found that FeNO plays an important role as a biomarker in severe asthmatic patients undergoing biologic treatment. Higher baseline FeNO levels are associated with better clinical control and lower exacerbation rates. FeNO during biologic treatment can help predict clinical worsening and differentiate eosinophilic from non-eosinophilic exacerbations.
Letter
Critical Care Medicine
Jonas C. Schupp, Edward P. Manning, Maurizio Chioccioli, Jan C. Kamp, Leonard Christian, Changwan Ryu, Erica Herzog, Mark P. Kuehnel, Antje Prasse, Naftali Kaminski, Danny D. Jonigk, Robert J. Homer
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Editorial Material
Medicine, General & Internal
Jannik Ruwisch, Tobias Welte, Antje Prasse
DEUTSCHE MEDIZINISCHE WOCHENSCHRIFT
(2023)
Article
Biochemistry & Molecular Biology
Miriana d'Alessandro, Laura Bergantini, Sara Gangi, Paolo Cameli, Martina Armati, Matteo Fanetti, Fabrizio Mezzasalma, Stefano Baglioni, Elena Bargagli
Summary: This study aimed to evaluate and compare T, B, and NK cell subsets in the alveolar compartment, lymph nodes, and bloodstream of sarcoidosis patients. The results showed enrichment of certain cell populations in the bloodstream, alveolar compartment, and lymph nodes. This study further supports the multisystemic nature of sarcoidosis and highlights the low level of immune cells in the peripheral blood of patients. Overall, the study has significant importance in understanding the immune responses associated with sarcoidosis development and progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laura Bergantini, Miriana d'Alessandro, Sara Gangi, Francesco Bianchi, Paolo Cameli, Beatrice Perea, Martina Meocci, Gaia Fabbri, Sofia Marrucci, Moftah Ederbali, Elena Bargagli
Summary: Coronavirus disease 2019 (COVID-19) may lead to a chronic syndrome in some patients even after the infection is resolved. Persistent cytokine dysregulation can cause long-lasting inflammation and tissue damage, resulting in a variety of debilitating symptoms. This study evaluated the value of serum biomarkers in predicting severity, prognosis, and development of post-COVID lung sequelae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Sandra Freitag-Wolf, Jonas C. Schupp, Bjoern C. Frye, Annegret Fischer, Raihanatul Anwar, Robert Kieszko, Violeta Mihailovic-Vucinic, Janusz Milanowski, Dragana Jovanovic, Gernot Zissel, Elena Bargagli, Paola Rottoli, Dragos Bumbacea, Rene Jonkers, Ling-Pei Ho, Karoline I. Gaede, Anna Dubaniewicz, Ben G. Marshall, Andreas Guenther, Martin Petrek, Michael P. Keane, Sigridur O. Haraldsdottir, Francesco Bonella, Christian Grah, Tatjana Peros-Golubicic, Zamir Kadija, Stefan Pabst, Christian Grohe, Janos Strausz, Martina Safrankova, Ann Millar, Jiri Homolka, Wim A. Wuyts, Lisa G. Spencer, Michael Pfeifer, Dominique Valeyre, Venerino Poletti, Hubertus Wirtz, Antje Prasse, Stefan Schreiber, Astrid Dempfle, Joachim Mueller-Quernheim
Summary: This study investigated the association of genetic markers with different phenotypes using meta-analysis. The results showed no significant genetic association in the overall cohort, but confirmed the association of acute onset with TNF and reported a new association with HLA polymorphism in the Serbian sub-cohort. The study also suggested that gene-environment interactions may influence the clinical phenotype and different geographic regions may have distinct genetic links and environmental factors.
FRONTIERS IN MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Laura Bianchi, Isabella Damiani, Silvia Castiglioni, Alfonso Carleo, Rossana De Salvo, Clara Rossi, Alberto Corsini, Stefano Bellosta
Summary: Cigarette smoke affects the smooth muscle cells in the aorta, leading to inflammation and reduced contraction. Mass spectrometry analysis revealed significant changes in protein abundance in human smooth muscle cells exposed to cigarette smoke, involving pathways related to inflammatory cytokines and interferon. Gene expression and protein abundance data identified KLF4 and PKR as the most relevant factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
