Journal
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
Volume 21, Issue 4, Pages -Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JSTQE.2014.2382978
Keywords
Ultra-fast laser inscription; microfluidic/optofluidic imaging; high-throughput imaging; glass microfluidics
Categories
Funding
- UKIERI Project Femtosecond Laser-Micromachined Microfluidics
- Integrated Surface Enhanced Raman Scattering Sensors for Point-of-Care Diagnostics
- EPSRC [EP/J500227/1]
- Biotechnology Ignition Grant of BIRAC
- EPSRC [EP/G030227/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [1284010, EP/G030227/1] Funding Source: researchfish
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Microfluidic/optofluidic microscopy is a versatile modality for imaging and analyzing properties of cells/particles while they are in flow. In this paper, we demonstrate the integration of fused silica microfluidics fabricated using femtosecond laser machining into optofluidic imaging systems. By using glass for the sample stage of our microscope, we have exploited its superior optical quality for imaging and bio-compatibility. By integrating these glass microfluidic devices into a custom-built bright field microscope, we have been able to image red blood cells in flow with high-throughputs and good fidelity. In addition, we also demonstrate imaging as well as detection of fluorescent beads with these microfluidic devices.
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