Article
Virology
Mingxia Li, Liping Zhang, Li Pan, Peng Zhou, Ruiming Yu, Zhongwang Zhang, Jianliang Lv, Huichen Guo, Yonglu Wang, Sa Xiao, Xinsheng Liu
Summary: In this study, the researchers found that nicotinamide (NAM) effectively inhibits the replication of PEDV and PDCoV by primarily targeting their RNA and protein synthesis processes. The activation of the ERK1/2/MAPK pathway and downregulation of transcription factors also contribute to the inhibition of virus replication. This suggests that NAM could be a promising antiviral candidate not only for swine intestinal coronaviruses but also for other human and animal coronaviruses.
Article
Veterinary Sciences
Jianpeng Chen, Rongchao Liu, Huaicheng Liu, Jing Chen, Xiaohan Li, Jianfeng Zhang, Bin Zhou
Summary: Multiplex quantitative PCR (qPCR) assay can detect and differentiate PEDV, TGEV, and PDCoV, providing a tool for active prevention and control of swine enteric coronaviruses. The positive rates for PEDV, TGEV, and PDCoV were 19.70%, 0.87%, and 10.17% respectively, with mixed infection rates ranging from 3.25% to 23.16%.
VETERINARY SCIENCES
(2023)
Article
Microbiology
Zhiwei Li, Zhiqian Ma, Linfang Dong, Ting Yang, Yang Li, Dian Jiao, Weiguo Han, Haixue Zheng, Shuqi Xiao
Summary: This study confirms that the S gene is the decisive gene for PEDV adaptability to monkey Vero cells and porcine LLC-PK1 cells. The exchange of S1 and S2 subunits in adaptive strains does not enable PEDV to adapt to cells, and the 803L and 976H residues in the S2 subunit are critical for rCH/SX/2016-S1(HNXP)+S3(HNXP) adaptation to Vero cells. These findings highlight the crucial role of the PEDV S protein in cell tropism and suggest the potential role of coronaviruses S protein in cross-species transmission. Additionally, this study provides unique insights into identifying PEDV functional receptors and developing PEDV and other coronaviruses vaccines.
Article
Veterinary Sciences
Pei-Hua Wang, Ya-Qian Li, Yuan-Qing Pan, Yan-Yan Guo, Fan Guo, Rui-Zhu Shi, Li Xing
Summary: This study analyzed full-length spike genes of PEDVs isolated in China from 2007 to 2019 and identified 28 potential recombination events, leading to the emergence of a new evolutionary branch GI-c during 2016-2018. Amino acid variations in the spike protein could affect 20 potential linear B cell epitopes, demonstrating dramatic antigen drift in PEDVs circulating in China.
VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Yan Li, Jia-Wei Niu, Xia Zhou, Pin-Pin Chu, Kun-Li Zhang, Hong-Chao Gou, Dong-Xia Yang, Jian-Feng Zhang, Chun-Ling Li, Ming Liao, Shao-Lun Zhai
Summary: A TaqMan probe-based multiplex real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) method was developed to simultaneously detect PEDV, TGEV, and PDCoV in pigs. The method showed high sensitivity and specificity with a detection limit of 2.95 x 10(0) copies/mu l for each virus. The assay of 160 clinical samples revealed positive rates of 38.13%, 1.88%, and 5.00% for PEDV, TGEV, and PDCoV, respectively. The multiplex qRT-PCR method had a 100% positive coincidence rate compared to single-reaction qRT-PCR. This method is important for clinical monitoring and control of porcine enteric diarrhea viruses.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Veterinary Sciences
Hejie Wang, Yibin Qin, Wu Zhao, Tingting Yuan, Chunjie Yang, Xue Mi, Ping Zhao, Ying Lu, Bingxia Lu, Zhongwei Chen, Ying He, Cui Yang, Xianfeng Yi, Zhuyue Wu, Ying Chen, Zuzhang Wei, Weijian Huang, Kang Ouyang
Summary: A novel porcine deltacoronavirus (PDCoV) strain, CHN/GX/1468B/2017, isolated in China, is closely related to the Southeast Asia strain with distinct genetic characteristics and pathogenicity, enriching the international information on the genetic diversity of PDCoV.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Xiaoliang Hu, Yuexiao Lian, Yucan He, Xiangxiao Liu, Zhige Tian, Yi Dai, Mengyuan Liu, Huayan Fan, Yue Shi, Feng Cong
Summary: A variant strain of PEDV, SC-YB73, was successfully isolated and identified in this study. Sequence analysis revealed a six-nucleotide insertion in the E gene, which has not been previously detected in PEDV strains. Phylogenetic analysis classified SC-YB73 in the widely prevalent GII-a variant subgroup in the Chinese pig population. Recombination analysis indicated that SC-YB73 originated from the recombination of GDS47, US PEDV prototype-like strains TW/Yunlin550/2018, and COL/Cundinamarca/2014.
Article
Virology
Claudia Kristen-Burmann, Peter Rogger, Ines Berenguer Veiga, Stefanie Riebesehl, Julie Rappe, Nadine Ebert, Carmen A. A. Sautter, Jenna N. N. Kelly, Hanspeter Stalder, Rosina Ehmann, Michael Huber, Horst Posthaus, Nicolas Ruggli, Volker Thiel, Gergely Tekes
Summary: In this study, a reverse genetics system was developed for a highly virulent PEDV strain, which can be used for studying pathogenesis and generating vaccines. The spike gene of PEDV was found to be an important factor in virulence, and recombination between different coronaviruses can lead to the emergence of similar viruses.
JOURNAL OF VIROLOGY
(2023)
Article
Immunology
Jieshi Yu, Chithra Sreenivasan, Tirth Uprety, Rongyuan Gao, Chen Huang, Ella J. Lee, Steven Lawson, Julie Nelson, Jane Christopher-Hennings, Radhey S. Kaushik, Eric Nelson, Diego G. Diel, Ben M. Hause, Feng Li, Dan Wang
Summary: Immunization with a vaccine containing PEDV spike protein induced high levels of neutralizing antibodies in mice and piglets, but resulted in worsened symptoms and increased virus replication in piglets. These findings highlight a previously unrecognized challenge in PEDV vaccine research with implications for coronavirus vaccine development.
Article
Biochemical Research Methods
Gen Li, Miaoli Wu, Jinhui Li, Weiyou Cai, Yongsheng Xie, Guangbing Si, Li Xiao, Feng Cong, Dongsheng He
Summary: The study aimed to establish a rapid detection method for PEDV and PDCoV using duplex RT-RPA, which showed high specificity and sensitivity for the viruses, providing an effective alternative for their detection.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Infectious Diseases
Honglei Zhang, Fangfang Han, Xiaoguang Yan, Lintao Liu, Xiangli Shu, Hui Hu
Summary: Research on PEDV strains in Henan, China revealed high infection rates, particularly affecting suckling piglets, and potentially caused by different PEDV variants. The S gene of PEDV Henan strains showed amino acid mutations that could alter virus antigenicity, leading to poor efficacy of traditional vaccines.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Agriculture, Dairy & Animal Science
Jiaguo Bai, Chen Du, Ying Lu, Ruomu Wang, Xueli Su, Kechen Yu, Qiuying Qin, Ying Chen, Zuzhang Wei, Weijian Huang, Kang Ouyang
Summary: This study collected and detected 673 diarrhea samples from 143 pig farms in Guangxi during 2017-2022 for PEDV. The results showed an increased number of strains in the G2c subgroup from 2019 onwards. Bayesian analysis revealed that Guigang may have been the epicenter of PEDVs in Guangxi. Guigang was identified as the primary hub from which PEDVs spread via two routes, namely Guigang-Wuzhou and Guigang-Laibin. Moreover, two strains originated from intra-genogroup recombination. This study provides new insights into the prevalence, genetic characteristics and evolutionary profiles of PEDV strains in China.
Article
Virology
Zhe Jiao, Jixiang Liang, Yilin Yang, Yang Li, Zhishan Yan, Guangli Hu, Changqin Gu, Xueying Hu, Guofu Cheng, Guiqing Peng, Wanpo Zhang
Summary: The coinfection of porcine deltacoronavirus and porcine epidemic diarrhea virus can alter the tropism of PEDV and potentially impact the outcome of the disease in piglets. This animal model provides a valuable tool for studying the pathogenesis and vaccination of viral coinfection in piglets in the future.
Review
Virology
Pan Tang, Enhui Cui, Yihong Song, Ruoqian Yan, Jingyu Wang
Summary: Porcine deltacoronavirus (PDCoV) is a significant enteropathogenic pathogen causing economic losses to the global pork industry. This review updates the current information on PDCoV characteristics, epidemiology, geographical distribution, and vaccine development, with a focus on China's prevalence and vaccine research. The comprehensive understanding of PDCoV infection provided in this review will aid in establishing new strategies for global virus control.
ARCHIVES OF VIROLOGY
(2021)
Article
Microbiology
Dongliang Li, Yongtao Li, Yunchao Liu, Yumei Chen, Wenqiang Jiao, Hua Feng, Qiang Wei, Jucai Wang, Yuhang Zhang, Gaiping Zhang
Summary: Porcine epidemic diarrhea virus (PEDV) is a major pathogen causing diarrhea and high mortality in newborn piglets, with recombination and mutation being the main driving forces of its genetic evolution and diversity. A recent study identified a novel PEDV isolate (HNAY) with unique insertions and mutations in its S1 gene, suggesting potential differences in pathogenicity compared to other strains. The findings contribute to a better understanding of PEDV genetic evolution and molecular pathogenesis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Chang Liu, Yunchao Liu, Hongying Chen, Hua Feng, Yumei Chen, Yinbiao Wang, Juan Wang, Dongmin Liu, Ruiguang Deng, Gaiping Zhang
ARCHIVES OF VIROLOGY
(2018)
Article
Biochemistry & Molecular Biology
Yapeng Song, Qiang Wei, Yunchao Liu, Hua Feng, Yumei Chen, Yanwei Wang, Yilin Bai, Guangxu Xing, Ruiguang Deng, Gaiping Zhang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Biochemistry & Molecular Biology
Chang Liu, Hua Feng, Yunchao Liu, Yumei Chen, Suzhen Yang, Ruiguang Deng, Gaiping Zhang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Veterinary Sciences
Guanmin Zheng, Qingxia Lu, Fangyu Wang, Guangxu Xing, Hua Feng, Qianyue Jin, Zhenhua Guo, Man Teng, Huifang Hao, Dongliang Li, Xin Wei, Yuhang Zhang, Ruiguang Deng, Gaiping Zhang
BMC VETERINARY RESEARCH
(2020)
Article
Immunology
Teng Zhang, Yunchao Liu, Yumei Chen, Aiping Wang, Hua Feng, Qiang Wei, Enmin Zhou, Gaiping Zhang
Article
Veterinary Sciences
Chang Liu, Yunchao Liu, Hua Feng, Baolei Zhao, Yumei Chen, Huimin Huang, Pan Wang, Ruiguang Deng, Gaiping Zhang
BMC VETERINARY RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Teng Zhang, Yunchao Liu, Yumei Chen, Jucai Wang, Hua Feng, Qiang Wei, Shuangshuang Zhao, Suzhen Yang, Hongfang Ma, Dongmin Liu, Gaiping Zhang
Summary: The study identified a novel type I interferon IFN-88 that exhibited significant antiviral effects against pseudorabies virus infection and increased the expression of IFN-stimulated genes to resist viral invasion. This highlights the potential of IFN-88 as an antiviral agent for preventing PRV infection and expanding the function of IFN antiviral drugs in the future.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Aiping Wang, Jinran Du, Hua Feng, Jingming Zhou, Yumei Chen, Yankai Liu, Min Jiang, Rui Jia, Yuanyuan Tian, Gaiping Zhang
Summary: This study successfully cloned the BTV1 VP2 gene and expressed the protein in insect cells, leading to the production of effective vaccines. Three anti-BTV1 VP2 monoclonal antibodies were generated, and a novel B-cell epitope on the VP2 protein was identified, laying a foundation for the development of diagnostic and prophylactic methods for bluetongue disease.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Dongliang Li, Yongtao Li, Yunchao Liu, Yumei Chen, Wenqiang Jiao, Hua Feng, Qiang Wei, Jucai Wang, Yuhang Zhang, Gaiping Zhang
Summary: Porcine epidemic diarrhea virus (PEDV) is a major pathogen causing diarrhea and high mortality in newborn piglets, with recombination and mutation being the main driving forces of its genetic evolution and diversity. A recent study identified a novel PEDV isolate (HNAY) with unique insertions and mutations in its S1 gene, suggesting potential differences in pathogenicity compared to other strains. The findings contribute to a better understanding of PEDV genetic evolution and molecular pathogenesis.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Virology
Hua Feng, Joaquim Segales, Fangyu Wang, Qianyue Jin, Aiping Wang, Gaiping Zhang, Giovanni Franzo
Summary: An overall analysis of codon usage bias for Chinese porcine circoviruses (PCVs) was performed, revealing the codon usage patterns and their relationship with the host, as well as the involvement of natural selection and mutation pressure in shaping these patterns. The study also showed that PCV-4 may be more adapted to its host compared to other PCVs. These insights contribute to a better understanding of the molecular evolution of PCVs.
Article
Microbiology
Rui Jia, Gaiping Zhang, Yilin Bai, Hongliang Liu, Yumei Chen, Peiyang Ding, Jingming Zhou, Hua Feng, Mingyang Li, Yuanyuan Tian, Aiping Wang
Summary: In this study, monoclonal antibodies against the ASFV CD2V protein were developed, and B cell epitopes were identified. This provides efficient epitopes for the development of ASFV subunit vaccines.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Qian Xu, Fangyu Wang, Wenqiang Jiao, Mengting Zhang, Guangxu Xing, Hua Feng, Xuefeng Sun, Man Hu, Gaiping Zhang
Summary: In this study, affinity peptides specifically recognizing the PEDV S1 C-terminal domain (CTD) protein were screened using molecular docking technology for the first time. Experimental results showed that the affinity peptides had significant antiviral effects and were non-toxic to cells. This study provides a new method for the development of antiviral drugs for PEDV.
Article
Biochemistry & Molecular Biology
Yunchao Liu, Jucai Wang, Yumei Chen, Aiping Wang, Qiang Wei, Suzhen Yang, Hua Feng, Shujun Chai, Dongmin Liu, Gaiping Zhang
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Chemistry, Multidisciplinary
Suzhen Yang, Yaning Sun, Jifei Yang, Yunchao Liu, Hua Feng, Gaiping Zhang
Article
Veterinary Sciences
R. Mischke, K. Rumstedt, S. O. Hungerbuehler, K. Rohn, M. Schmicke
Summary: Contrast-enhanced ultrasound (CEUS) can provide an additional tool for the diagnosis of hypothyroidism in dogs and support its differentiation from non-thyroidal illness. Sedation has limited impact on CEUS results.
RESEARCH IN VETERINARY SCIENCE
(2024)
Review
Veterinary Sciences
Lucas Petitemberte de Souza, William Borges Domingues, Eduardo Bierhals Blodorn, Leandro da Silva Nunes, Hadassa Gabriela Ortiz, Eliza Rossi Komninou, Vinicius Farias Campos
Summary: This study performed a systematic review of the differential expression of sperm microRNAs in bulls related to fertility. A total of 182 unique differentially expressed miRNAs were identified, among which 49 miRNAs were found in common between at least two studies. The results suggest that miRNAs such as miR-10a, -10b, -103, -15b, -122, -125b, -126-5p, -151-5p, -193a-5p, -196a, -27a-5p, and -99b may serve as potential universal biomarkers for assessing the reproductive potential of males.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Wonhee Cha, Katinca Fungbrant, Giulio Grandi, Ylva Persson
Summary: This study investigated the prevalence and development of hair loss in outdoor cattle in Sweden. It found a relatively high hair loss rate, with the occurrence of hair loss increasing over time during winters, suggesting a contagious etiology. The study also identified preventative delousing before November, keeping animals clean, and maintaining small group sizes as effective measures to reduce the risk of hair loss, while older age and access to bedding materials increased the risk.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Sheeba Thomas, David Francis Lappin, David Bennett, Christopher Nile, Marcello Pasquale Riggio
Summary: This study found that Feline odontoclastic resorptive lesion (FORL) can be divided into two subgroups, with FORL-1 subgroup having a less diverse oral microbiome and higher levels of proinflammatory cytokines and chemokines, which may be associated with the severity and activity of the disease.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
S. L. Gayathri, M. Bhakat, T. K. Mohanty
Summary: This study used infrared thermography (IRT) to identify mastitis in Sahiwal cows. The results showed significant differences in the mean values of udder and teat surface temperature between healthy, subclinical mastitis (SCM), and clinical mastitis (CM) cases during different seasons. IRT is an efficient tool for the early identification of subclinical mastitis.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Zeynep Ozdemir Kutahya, Busra Aslan Akyol, Kubra Deliklitas, Cengiz Gokbulut
Summary: In this study, the pharmacokinetic behavior of Eprinomectin was studied in two different goat breeds. The results showed no significant differences in plasma concentration between the breeds, but the clearance from the body may vary. High concentration of Eprinomectin in feces is beneficial against gastrointestinal parasites but poses an ecotoxicological risk to the environment.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Mohammad Ashrafzadeh-Shiraz, Mousa Tavassoli, Bahram Dalir-Naghadeh, Alireza Sazmand
Summary: This study evaluated the efficacy of fenbendazole and ivermectin on strongyles and Parascaris sp. infecting adult riding horses in different regions of Iran. The results demonstrated varying degrees of resistance of these parasites to both anthelminthics. It emphasizes the urgency of educating horse owners about the importance of a test-and-treatment strategy and suggests evaluating the efficacy of different anthelminthics to choose the most effective treatment in each region.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Moises Gonzalvez, Clara Munoz-Hernandez
Summary: The study evaluated the use of R software in research articles addressing the study of wildlife, showing an increasing trend in the number of publications over time and a wide geographical distribution globally.
RESEARCH IN VETERINARY SCIENCE
(2024)
