Review
Biochemistry & Molecular Biology
Irma Virant-Klun, Senka Imamovic-Kumalic, Bojana Pinter
Summary: Exposure to endocrine-disrupting chemicals can lead to oxidative stress and disruption of the endocrine system, affecting human reproduction and development. This review examines the association between endocrine disruptors and oxidative stress-related male infertility. It finds that bisphenols and phthalates have detrimental effects on semen quality and sperm DNA integrity.
Article
Obstetrics & Gynecology
Weijian Zhu, Lei Jiang, Chengshuang Pan, Junhui Sun, Xuefeng Huang, Wuhua Ni
Summary: The study evaluated DNA methylation patterns in sperm from men with differential levels of sperm DNA fragmentation index (DFI). The results showed that high DFI in male sperm was associated with significantly altered methylation in specific CpG fragments corresponding to nine genes. This suggests that sperm DNA damage may influence DNA methylation patterns in human sperm.
FERTILITY AND STERILITY
(2021)
Article
Andrology
Kamil Gill, Tomasz Machalowski, Patryk Harasny, Marta Grabowska, Ewa Duchnik, Malgorzata Piasecka
Summary: This study aimed to investigate the distribution and risk of sperm DNA fragmentation (SDF) and oxidative stress in semen in asthenozoospermic men. The results showed that asthenozoospermic men had lower basic semen parameters compared to the control and nonasthenozoospermic groups. The prevalence and risk of high levels of DNA damage and oxidative stress were significantly higher in men with poor sperm motility. The risk of sperm DNA damage and oxidative stress in asthenozoospermic men was much higher than that in control subjects and nonasthenozoospermic men.
Article
Agriculture, Dairy & Animal Science
Eneiva Carla Carvalho Celeghini, Maira Bianchi Rodrigues Alves, Rubens Paes de Arruda, Gabriela Marques de Rezende, Shirley Andrea Florez-Rodriguez, Manoel Francisco de Sa Filho
Summary: This study investigated the efficiency of CellROX Deep Red (R) and Orange (R) probes in detecting ROS in bovine sperm cells, finding that higher sperm ROS levels detected by CellROX Red (R) might be associated with low fertility samples. The coefficients of determination for Red and Orange probes were 0.61 and 0.56, respectively, indicating their effectiveness in detecting ROS. Overall, both CellROX Red (R) and Orange (R) were shown to be efficient in detecting ROS in bovine spermatozoa.
ANIMAL BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Stefan Banas, Filip Benko, Michal Duracka, Norbert Lukac, Eva Tvrda
Summary: EPC has been shown to improve the quality parameters of cryopreserved bovine sperm, reduce the proportion of capacitated sperm, and decrease the production of reactive oxygen species. Additionally, EPC protects the integrity of the sperm membrane and membrane channels crucial for the sperm capacitation process. These findings suggest that EPC can enhance the structural integrity and functional activity of spermatozoa after cryopreservation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Haiyang Zhao, Tingting Zhao, Jihong Yang, Qianqian Huang, Hua Wu, Yueyun Pan, Hui Wang, Yun Qian
Summary: Oxidative stress is a major factor in male infertility, and oral administration of antioxidants has been proven to improve sperm quality. This study used Pex3-knockout mice as a model to compare the efficacy of three types of traditional Chinese medicine granules for rescuing male reproductive function, and found that Epimedium was the most effective. The study also explored the mechanism by which Epimedium improves dyszoospermia, and found that it partially corrects the associated phenotype by affecting P16 expression levels.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Usha Punjabi, Ilse Goovaerts, Kris Peeters, Diane De Neubourg
Summary: Antioxidant therapy should only be used for infertile patients showing signs of oxidative stress. However, there is no consensus on the measurement of the primary endpoint and the assay to be used. This study analyzed the formation of 8-hydroxy-2'-deoxyguanosine (8-OHdG), an early marker of sperm DNA oxidation (SDO), in male infertility patients. The results showed that SDO was associated with semen volume, sperm concentration, leucocytes and round cells, but not with age, body mass index, sperm DNA fragmentation or oxidative stress. Further validation and optimization of the methodology for SDO is needed before it can be used to monitor antioxidant therapy in male infertility.
Article
Nanoscience & Nanotechnology
Asmaa M. Shehata, Fatma M. S. Salem, Eiman M. El-Saied, Sahar S. Abd El-Rahman, Mohamed Y. Mahmoud, Peter A. Noshy
Summary: The study showed that exposure to Ag-NPs had adverse effects on the male reproductive system, affecting sperm motility, morphology, and concentration, inducing oxidative stress and lipid peroxidation in testicular tissue, and decreasing serum hormone levels. However, coadministration of Zn-NPs ameliorated most of the toxic effects of Ag-NPs through their antioxidative capacity.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Review
Obstetrics & Gynecology
Robert John Aitken
Summary: Spermatogonial stem cells have a low level of spontaneous mutation, which is heavily affected by paternal age and mechanisms related to aberrant DNA damage repair. Mutations affecting a key signal transduction pathway can greatly increase the frequency of de-novo mutations, leading to clonal expansion of mutant germ cells in the testes of older males. This model explains the origin of certain developmental disorders, but cannot explain the majority of de-novo mutations where no selective advantage is apparent.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Agriculture, Dairy & Animal Science
Michal Duracka, Kamila Husarcikova, Mikulas Jancov, Lucia Galovicova, Miroslava Kacaniova, Norbert Lukac, Eva Tvrda
Summary: This study simulated the effects of staphylococcal infection on bovine semen quality, finding that the presence of Staphylococcus can lead to oxidative stress, resulting in sperm DNA fragmentation, changes in mitochondrial membrane potential, and decreased sperm motility.
Article
Biochemistry & Molecular Biology
Elisa Hug, Pauline Villeneuve, Stephanie Bravard, Areski Chorfa, Christelle Damon-Soubeyrand, Stephen G. Somkuti, Aron Moazamian, R. John Aitken, Parviz Gharagozloo, Joel R. Drevet, Fabrice Saez
Summary: Routine exposure to endocrine-disrupting chemicals (EDCs), such as DBP and BPAF, can lead to decreased sperm quality and DNA damage, while not affecting sperm viability or testicular structures. The observed damage is likely caused by oxidative stress induced by the pro-oxidant properties of the EDCs, as it can be largely prevented by co-administering antioxidants. These findings highlight the importance of understanding the effects of EDCs on male reproductive health.
Article
Biochemistry & Molecular Biology
Costanza Calamai, Oumaima Ammar, Viktoria Rosta, Ginevra Farnetani, Salvatore Zimmitti, Lisa Giovannelli, Linda Vignozzi, Csilla Krausz, Monica Muratori
Summary: Cancer significantly increases sperm DNA fragmentation (sDF) and oxidative stress levels. The induction of viable oxidative stress is even higher in cancer patients compared to normozoospermic subfertile patients and healthy donors. There is a correlation between sDF and viable oxidative stress in all subjects, but not in cancer patients alone. Detecting oxidative stress could help manage the reproductive issues of cancer patients.
Article
Agriculture, Dairy & Animal Science
Zafer Dogu, Erdinc Sahinoz, Faruk Aral, Ismail Koyuncu, Ozgur Yuksekdag
Summary: This study found that increasing levels of inositol significantly improved sperm motility rate and duration, decreased oxidant status and DNA damage, and reduced the percentage of apoptotic sperm in Mesopotamian Catfish post-thaw process. These results indicate the positive effects of supplemental inositol on enhancing sperm cryopreservation efficiency in Silurus triostegus.
Review
Biochemistry & Molecular Biology
Francisca Felix, Catarina C. V. Oliveira, Elsa Cabrita
Summary: The study focuses on the effects of novel antioxidants in protecting fish cells, highlighting the importance of antioxidants in fish reproduction and their potential in improving gamete quality. The review discusses various types of antioxidants and their mechanisms of action, with a particular emphasis on the protective role of melatonin in preventing oxidative stress damage on fish spermatozoa.
Review
Obstetrics & Gynecology
Filipe Tenorio Lira Neto, Matheus Roque, Sandro C. Esteves
Summary: This study found that varicocelectomy can reduce sperm deoxyribonucleic acid fragmentation rates in infertile men with clinical varicocele. Men with elevated preoperative SDF levels showed a more significant treatment effect after the surgery.
FERTILITY AND STERILITY
(2021)
Article
Obstetrics & Gynecology
Myriam Ruiz, Esther Santamaria-Lopez, Victor Blasco, Maria Jose Hernaez, Cinzia Caligara, Antonio Pellicer, Manuel Fernandez-Sanchez, Nicolas Prados
REPRODUCTIVE SCIENCES
(2020)
Review
Medicine, General & Internal
Victor Blasco, Francisco M. Pinto, Cristina Gonzalez-Ravina, Esther Santamaria-Lopez, Luz Candenas, Manuel Fernandez-Sanchez
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Obstetrics & Gynecology
Carlos Simon, Carlos Gomez, Sergio Cabanillas, Iavor Vladimirov, Gemma Castillon, Juan Giles, Kubra Boynukalin, Necati Findikli, Mustafa Bahceci, Israel Ortega, Carmina Vidal, Miyako Funabiki, Alexandra Izquierdo, Lourdes Lopez, Susana Portela, Nilo Frantz, Marcos Kulmann, Sagiri Taguchi, Elena Labarta, Francisco Colucci, Shari Mackens, Xavier Santamaria, Elkin Munoz, Saul Barrera, Juan Antonio Garcia-Velasco, Manuel Fernandez, Marcos Ferrando, Maria Ruiz, Ben W. Mol, Diana Valbuena
REPRODUCTIVE BIOMEDICINE ONLINE
(2020)
Article
Obstetrics & Gynecology
Alessio Miceli, Manuel Fernandez-Sanchez, Jose-Luis Duenas-Diez
Summary: This study evaluated the efficacy and safety of long-term continuous use of a ring pessary, with most patients successfully using it for over 2 years. Some patients experienced minor adverse events during long-term use, but most issues were temporary in nature.
INTERNATIONAL UROGYNECOLOGY JOURNAL
(2021)
Article
Medicine, General & Internal
Julia Ramos, Cinzia Caligara, Esther Santamaria-Lopez, Cristina Gonzalez-Ravina, Nicolas Prados, Francisco Carranza, Victor Blasco, Manuel Fernandez-Sanchez
Summary: This study compared the diagnostic accuracy of HyFoSy and HSG in tubal patency testing, showing a moderate concordance of 72.6% between the two tests, with HyFoSy causing less pain. Therefore, for patients with low risk of tubal disease, HyFoSy could be the preferred diagnostic option.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biology
Thanh Huong Nguyen Ho-Bouldoires, Kevin Sollier, Laura Zamfirov, Florence Broders-Bondon, Demosthene Mitrossilis, Sebastian Bermeo, Coralie L. Guerin, Anna Chipont, Gabriel Champenois, Renaud Leclere, Nicolas Andre, Laurent Ranno, Aude Michel, Christine Menager, Didier Meseure, Charlie Demene, Mickael Tanter, Maria Elena Fernandez-Sanchez, Emmanuel Farge
Summary: The study found that high frequency pulsatile mechanical stresses maintain the physiological level of mice colon stem cells through the mechanosensitive Ret kinase. However, when stimulated by permanent tumor growth pressure, the stem cell levels increase pathologically and undergo hyperproliferation and tumorigenic transformation. Additionally, Ret activation in human colon cancer tissue is associated with tumor grade.
COMMUNICATIONS BIOLOGY
(2022)
Article
Cell Biology
Cristina Gonzalez-Ravina, Esther Santamaria-Lopez, Alberto Pacheco, Julia Ramos, Francisco Carranza, Lucia Murria, Ana Ortiz-Vallecillo, Manuel Fernandez-Sanchez
Summary: This study analyzed the clinical outcomes of magnetic-activated cell sorting (MACS) on donor intrauterine insemination (D-IUI) cycles. The results showed no significant differences in gestation, live birth, or miscarriage rates between the MACS group and the control group. Further studies with a larger sample size are needed to evaluate the application of MACS in fertility clinics.
Article
Obstetrics & Gynecology
Victor Blasco, Francisco M. Pinto, Ainhoa Fernandez-Atucha, Nicolas Prados Dodd, Manuel Fernandez-Sanchez, Luz Candenas
Summary: Neurokinin B and its receptors have a significant role in the regulation of female reproduction. Abnormal expression of these molecules may be associated with decreased fertility in women with infertility, providing new insights for diagnosis and treatment.
REPRODUCTIVE SCIENCES
(2023)
Article
Endocrinology & Metabolism
Manuel Fernandez Sanchez, Per Larsson, Marcos Ferrando Serrano, Ernesto Bosch, Juan Antonio Garcia Velasco, Esther Santamaria Lopez, Bernadette Mannaerts
Summary: This study aimed to explore the efficacy and safety of individualized follitropin delta dosing based on AMH concentration and body weight in a long GnRH agonist protocol. The results showed that the cumulative live-birth rate was high with individualized follitropin delta dosing.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Obstetrics & Gynecology
Julia Ramos, Nuria Pellicer, Manuel Fernandez-Sanchez
Summary: HyFoSy has emerged as a new option for easier Fallopian tube assessment, with several advantages over other techniques. Despite this, professionals still prefer the traditional X-ray hysterosalpingography instead of indicating HyFoSy as a first-choice diagnostic tool.
REPRODUCTIVE BIOMEDICINE ONLINE
(2022)
Article
Reproductive Biology
Patrycja Kurowska, Ewa Mlyczynska, Julia Wajda, Konrad Krol, Karolina Pich, Patrycja Guzman, Aleksandra Greggio, Oliwia Szkraba, Malgorzata Opydo, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression of PNX-14 and GPR173 in ovarian tissue and the effects of PNX-14 on granulosa cell function. The results showed that PNX-14 can influence ovarian follicle growth by promoting granulosa cell proliferation, hormone secretion, and cell cycle progression.
REPRODUCTIVE BIOLOGY
(2024)
Article
Reproductive Biology
Sonia Srivastava, Swarnima Mishra, Gyan Babu, Banalata Mohanty
Summary: This study aimed to investigate the efficacy of PD149163 in preventing/ameliorating LPS-induced inflammation in the female reproductive system of mice. The results showed that PD149163 reduced/prevented the inflammation in the ovary and uterine horn, restored the balance of inflammatory cytokines and oxidative stress, and recovered the levels of reproductive hormones and corticosterone.
REPRODUCTIVE BIOLOGY
(2024)
Article
Reproductive Biology
Chen Zhang, Hongyan Cheng, Xue Ye, Heng Cui, Yi Li, Honglan Zhu, Xiaohong Chang
Summary: Extracellular matrix protein 1 (ECM1) is significantly upregulated in endometriosis tissues. Blocking ECM1 attenuates cell migration and invasion. ECM1 regulates cell migration, invasion, and cytoskeleton reorganization via the RhoC/ROCK1 signaling pathway.
REPRODUCTIVE BIOLOGY
(2024)