Article
Reproductive Biology
Guo Chen, Li Ren, Zhanglin Chang, Yuting Zhao, Yanwen Zhang, Dong Xia, Ruqian Zhao, Bin He
Summary: High glucose concentration-induced lysine acetylation participates in the regulation of boar spermatozoa motility and acrosome integrity during preservation.
Article
Cell Biology
Yongjie Xu, Qiu Han, Chaofeng Ma, Yaling Wang, Pengpeng Zhang, Cencen Li, Xiaofang Cheng, Haixia Xu
Summary: Comprehensive proteomics analysis of sperm from Yorkshire and Duroc boars revealed differential expression of proteins and phosphorylated proteins related to crucial physiological functions such as sperm development, motility, and energy metabolism. Proteins involved in axonemal dynein complex assembly and sperm-egg recognition were found to be significantly higher in Duroc spermatozoa, indicating potential differences in sperm function between the two boar breeds.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Josue Calderon-Calderon, Francisco Sevilla, Eduardo R. S. Roldan, Vinicio Barquero, Anthony Valverde
Summary: This study aimed to evaluate the effect of fat-soluble vitamin supplementation on the seminal quality of boars. The results showed that fat-soluble vitamin supplementation increased semen volume, motility, progressive motility, and velocity variables. Overall, dietary fat-soluble vitamin supplementation significantly improved the semen quality of boar ejaculates.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Bin Zhang, Yan Wang, Caihong Wu, Shulei Qiu, Xiaolan Chen, Bingyan Cai, Huimei Xie
Summary: In this study, the quality of boar spermatozoa at different steps of cryopreservation was evaluated, revealing that excessive ROS during freeze-thaw cycling can negatively affect sperm motility, plasma membrane integrity, mitochondrial activity, sperm chromatin structure, and apoptosis. Addition of the antioxidant NAC reversed these negative effects, highlighting the importance of ROS downregulation for successful frozen pig sperm production and livestock propagation.
BMC VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Elisa Giaretta, Beatrice Mislei, Felipe Martinez-Pastor, Salvatore Nesci, Marcella Spinaci, Giovanna Galeati, Chiara Nerozzi, Gaetano Mari, Carlo Tamanini, Diego Bucci
Summary: This study aimed to investigate the role of mitochondria in horse sperm motility and ROS production by incubating spermatozoa with specific inhibitors. The results showed that disruption of mitochondrial integrity led to an increase in mitochondrial ROS production, which negatively affected sperm function and survival.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Reproductive Biology
Hai Thanh Nguyen, Son Quang Do, Hiroshi Kobayashi, Takuya Wakai, Hiroaki Funahashi
Summary: The purpose of this study was to investigate the relationship between mitochondrial content of commercial frozen-thawed bull spermatozoa and motility. The results showed that MDCN and MC of frozen spermatozoa differ among sires, and are negatively correlated with HMMP and sperm motility parameters. It should be noted that the MDCN and MC of bull spermatozoa may not vary overall with the age of the sire, whereas those changes with age in some individuals and may affect sperm motility.
Article
Biochemistry & Molecular Biology
David F. Carrageta, Lais Freire-Brito, Barbara Guerra-Carvalho, Joao C. Ribeiro, Bruno S. Monteiro, Alberto Barros, Pedro F. Oliveira, Mariana P. Monteiro, Marco G. Alves
Summary: UCPs play a crucial role in regulating mitochondrial activity and ROS production. The expression and function of UCPs in human spermatozoa have been investigated, providing valuable insights into the mechanisms of male infertility.
Article
Multidisciplinary Sciences
Heiko Henning, Anne-Marie Luther, Lisa Hoefner-Schmiing, Dagmar Waberski
Summary: This single cell study found that semen samples from boars with a higher incidence of sperm with a retained cytoplasmic droplet (CD) had fewer sperm with CD but a higher total sperm count. Morphologically normal sperm from semen samples with and without CD had similar responses to motility stimulators and viability rates. The presence of CD in sperm did not affect sperm viability.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Nathalie Le Foll, Jean-Christophe Pont, Audrey L'Hostis, Thomas Guilbert, Frederic Bouillaud, Jean-Philippe Wolf, Ahmed Ziyyat
Summary: cFEE improves gamete interaction in humans by enhancing sperm movement parameters and increasing the percentage of hyperactivated sperm. However, it does not significantly impact the analyzed mitochondrial bioenergetic parameters, indicating that it may act through a more efficient use of energy resources.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Maria Serrano Albal, Marie Claire Aquilina, Louisa J. Zak, Peter J. Ellis, Darren K. Griffin
Summary: Vitrification of boar sperm by direct contact with liquid nitrogen is increasingly popular. Comparing the pearl and mini-straw methods of vitrification, the pearl method was found to better preserve the viability and motility of the sperm samples.
Article
Veterinary Sciences
Patricia Marini, Luciana Fernandez Beato, Fernando Cane, Juan Manuel Teijeiro
Summary: The addition of 2 mM ZnCl2 to diluted boar sperm did not affect sperm viability, motility, mitochondrial activity, DNA integrity or ROS levels. However, ZnCl2 improved acrosomal integrity and sperm kinematics.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Multidisciplinary Sciences
Akila Cooray, Mee Ree Chae, Tharaka Darshana Wijerathne, Dae Geun Kim, Jeongsook Kim, Chul Young Kim, Sung Won Lee, Kyu Pil Lee
Summary: This study aimed to investigate the effects of PJE on boar spermatozoa and potential underlying mechanisms. We found that PJE significantly increased motility, intracellular calcium concentration, and capacitation of the sperm. These results suggest that PJE has the potential to improve sperm quality.
Article
Genetics & Heredity
Anna A. A. Kapitonova, Vladimir I. I. Muronetz, Denis V. V. Pozdyshev
Summary: Sperm sexing is a technique commonly used in the dairy industry to sort sperm cells based on X or Y chromosomes. This study analyzed the characteristics of sexed semen compared to conventional semen and found that the enzymatic activity of GAPDHS was significantly higher in sexed samples. This enzyme plays a significant role in sperm motility and may contribute to enhancing the fertility characteristics of sexed semen.
Article
Reproductive Biology
Pawel Brym, Karolina Wasilewska-Sakowska, Marzena Mogielnicka-Brzozowska, Anna Mankowska, Lukasz Paukszto, Chandra S. Pareek, Wladyslaw Kordan, Stanislaw Kondracki, Leyland Fraser
Summary: This study identified polymorphic variants in the 5'-flanking regulatory regions of genes expressed in boar spermatozoa, which were found to be associated with semen freezability. These variants can influence the interactions of transcription factors with gene promoters, thereby affecting gene expression levels.
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Francesca Mancuso, Nunziata Barone, Iva Arato, Cinzia Lilli, Catia Bellucci, Marco Musmeci, Giovanni Luca, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: This study evaluated the expression of the follicle-stimulating hormone receptor (FSHR) in human spermatozoa and its effects on sperm function. The results showed that FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa, with no difference in localization between patients and controls. Incubation with FSH increased FSHR expression in patients. Furthermore, FSH incubation had a negative effect on sperm motility and mitochondrial function in both patients and controls, but more severely in controls. FSH also activated the AKT(473) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Mercedes Torres-Badia, Soraya Solar-Malaga, David Martin-Hidalgo, Ana Hurtado de Llera, Andrea Gomez-Candelo, Luis J. Garcia-Marin, Lauro Gonzalez-Fernandez, Maria J. Bragado
Summary: The study reveals that low concentrations of Roundup herbicide cause impaired sperm motility in pigs without affecting sperm viability. This adverse effect is likely due to a detrimental effect in the plasma membrane lipid organization and inhibition of phosphorylation of GSK3 beta and specific PKA substrates.
Article
Agriculture, Dairy & Animal Science
David Martin-Hidalgo, Beatriz Macias-Garcia, Lauro Gonzalez-Fernandez
Summary: Increasing sperm concentration stimulates capacitation and acrosome reaction in boars, accompanied by plasma membrane lipid disorganization and increased sperm viability.
JOURNAL OF REPRODUCTION AND DEVELOPMENT
(2022)
Article
Veterinary Sciences
Pablo Fernandez-Hernandez, Andres Domingo, Jesus Garcia-Marin, Lauro Gonzalez-Fernandez, Beatriz Macias-Garcia, Luis Jesus Garcia-Marin, Maria Julia Bragado
Summary: This study tested the effects of different metabolites found in equine oviductal fluid on the quality of frozen-thawed spermatozoa. The results showed that the addition of these metabolites did not have any effect on sperm parameters. Further research is needed to determine if the metabolites in the oviductal fluid have a significant impact on in vitro equine sperm capacitation.
JOURNAL OF EQUINE VETERINARY SCIENCE
(2022)
Review
Biology
Rebeca Serrano, Luis J. Garcia-Marin, Maria J. Bragado
Summary: Approximately 24% of men undergoing assisted reproductive technology (ART) have idiopathic male infertility, which cannot be accurately diagnosed by current standard semen analysis. Proteomics, especially phosphoproteomics, has provided valuable insights into the regulation of sperm functions, such as motility, and male fertility. Further studies combining proteomic techniques with other omics technologies could enhance our understanding of the molecular mechanisms underlying sperm physiology and quality.
Article
Obstetrics & Gynecology
David Martin-Hidalgo, Lauro Gonzalez-Fernandez, M. Julia Bragado, Luis J. Garcia-Marin, Marco G. Alves, Pedro F. Oliveira
Summary: YK 3-237 induces capacitation-related events in human spermatozoa, such as an increase in tyrosine phosphorylation levels and acrosome-reacted spermatozoa after the ionophore challenge. These effects are independent of protein lysine acetylation but depend on bicarbonate and calcium levels.
REPRODUCTIVE BIOMEDICINE ONLINE
(2023)
Article
Multidisciplinary Sciences
Mercedes Torres-Badia, Soraya Solar-Malaga, Rebeca Serrano, Luis J. Garcia-Marin, M. Julia Bragado
Summary: This study investigates the effects of the widely used herbicide Roundup on human spermatozoa. The results show that Roundup disrupts the lipid organization and integrity of sperm plasma membrane, reducing motility without affecting viability or functional parameters. Importantly, these adverse effects are caused by the surfactant in Roundup rather than glyphosate.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Mercedes Torres-Badia, David Martin-Hidalgo, Rebeca Serrano, Luis J. Garcia-Marin, Maria J. Bragado
Summary: This study compares the effects of BPA and its analog BPS on pig sperm, focusing on motility, intracellular signaling pathways, and functional parameters. The results show that BPS has a smaller and slower impact on sperm compared to BPA, but significantly affects mitochondrial activity at a concentration of 100 mu M. However, BPA leads to adverse effects on sperm motility, mitochondrial function, and intracellular oxidant production. These findings help explain the reduced sperm motility caused by BPA and suggest that the mechanisms triggered by BPS are different.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Veterinary Sciences
David Martin-Hidalgo, Soraya Solar-Malaga, Lauro Gonzalez-Fernandez, Jose Zamorano, Luis Jesus Garcia-Marin, Maria Julia Bragado
Summary: During sperm capacitation, SIRT1 activator YK 3-237 can promote pig sperm activation by increasing protein tyrosine phosphorylation, inducing acrosome reaction, and elevating intracellular pH and calcium levels.
VETERINARY RESEARCH COMMUNICATIONS
(2023)
Article
Cell Biology
Ana Romarowski, Jasna Fejzo, Saman Nayyab, David Martin-Hidalgo, Maria G. Gervasi, Melanie Balbach, Sara Violante, Ana M. Salicioni, Justin Cross, Lonny R. Levin, Jochen Buck, Pablo E. Visconti
Summary: We developed a new method for treating sperm in vitro, resulting in higher fertilization and embryo development rates. Energy-restricted sperm showed altered metabolite levels, and upon re-addition of glucose and capacitation, most metabolites recovered. Both control and energy-restricted sperm showed increased glycolysis and oxidative phosphorylation under capacitating conditions. Exogenously supplied glucose was converted into citrate, suggesting that glycolytic products can be converted into Krebs cycle metabolites in sperm cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
