Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 115, Issue 38, Pages 9432-9437Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.1712792115
Keywords
estradiol; DNA nanotechnology; eNOS; cell signaling; live imaging
Categories
Funding
- Human Frontier Science Program Research Grant [RGP0029/2014]
- National Center for Advancing Translational Sciences of the National Institutes of Health [UL1 TR000430]
- Materials Research Science and Engineering Center Grant [DMR-1420709]
- France and Chicago Collaborating in the Sciences
- University of Chicago Women's Board
- UChicago start-up funds
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Membrane-initiated steroid signaling (MISS) is a recently discovered aspect of steroidal control over cell function that has proved highly challenging to study due to its rapidity and ultrasensitivity to the steroid trigger [Chow RWY, Handelsman DJ, Ng MKC (2010) Endocrinology 151:2411-2422]. Fundamental aspects underlying MISS, such as receptor binding, kinetics of ion-channel opening, and production of downstream effector molecules remain obscure because a pristine molecular technology that could trigger the release of signaling steroids was not available. We have recently described a prototype DNA nanocapsule which can be programmed to release small molecules upon photoirradiation [Veetil AT, et al. (2017) Nat Nanotechnol 12:1183-1189]. Here we show that this DNA-based molecular technology can now be programmed to chemically trigger MISS, significantly expanding its applicability to systems that are refractory to photoirradiation.
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