Article
Chemistry, Physical
Baptiste Arbez, Marion Gardette, Christophe Gantzer, Neus Vila, Isabelle Bertrand, Sofiane El-Kirat-Chatel
Summary: This study investigates the adhesion of phage 187 on living Staphylococcus aureus cells using atomic force microscopy-based single-particle force spectroscopy. The addition of N-acetyl-D-glucosamine was found to decrease phage adhesion, suggesting its important role in phage 187 infection. The research also successfully identifies a major receptor required for phage 187 infection of S. aureus.
Article
Engineering, Environmental
Jing-Hua Tzeng, Chih-Huang Weng, Chun-Chieh Wang, Mon-Shu Ho, Li-Ting Yen, Jian-Yu Chen, Gulomjon Gaybullaev, Chakkrit Poonpakdee, Yao-Tung Lin
Summary: The research revealed that cell adhesion and deformations in cellular structure are the main mechanisms of bacterial damage under visible-light-responsive N-TiO2 inactivation. Penetration of NTiO2 particles through the cell membrane leads to outer cellular destruction, resulting in leakage of cellular K+ and lipid peroxidation. AFM and TXM provide valuable insights into nanoscale interactions between single cell and nano-materials for early diagnosis of cell damage.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Junso Fujita, Shogo Sugiyama, Haruna Terakado, Maho Miyazaki, Mayuki Ozawa, Nanami Ueda, Natsuko Kuroda, Shun-ichi Tanaka, Takuya Yoshizawa, Takayuki Uchihashi, Hiroyoshi Matsumura
Summary: FtsZ is a key protein in bacterial cell division, and high-speed AFM allows visualization of its filament formation and dissociation process. Analysis shows that FtsZt filaments have different growth and dissociation rates under different conditions, and prefer straight shapes compared to FtsZf filaments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Ping-Lian Ko, Chien-Kai Wang, Heng-Hua Hsu, Tse-Ang Lee, Yi-Chung Tung
Summary: This paper develops an integrated approach to characterize the anisotropic elasticity of endothelium under physiological-level fluid shear stress. The results show that the transverse elasticity of the endothelium in the direction parallel to the flow direction is about 70% higher than that in the perpendicular direction, and the transverse elasticities are estimated to be approximately 120 times larger than the vertical one. These results provide insights into the effects of fluid shear stress on the endothelium's elasticity and anisotropy.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Physical
Mohd Aidy Faizal Johari, Asmawan Mohd Sarman, Saiful Amri Mazlan, U. Ubaidillah, Nur Azmah Nordin, Siti Aishah Abdul Aziz, Norhasnidawani Johari, Nurhazimah Nazmi, Shahir Mohd Yusuf
Summary: The study focuses on the behavior of shear bands in magnetorheological (MR) solids, utilizing tools such as atomic force microscopy (AFM) to explore the characteristics of shear bands during stress relaxation and demonstrate the presence of localized affected zones. These findings provide important insights into understanding the formation and evolution of shear bands.
Article
Hematology
Oscar Negron, Miranda Weggeman, Jos Grimbergen, Emily G. Clark, Sara Abrahams, Woosuk S. Hur, Jaap Koopman, Matthew J. Flick
Summary: This study found that changing the ratio of naturally occurring fibrinogen variants in blood could offer significant therapeutic potential against bacterial infection and potentially other diseases.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Immunology
Jyotirmayee Dey, Soumya Ranjan Mahapatra, Pratima Singh, Swadheena Patro, Gajraj Singh Kushwaha, Namrata Misra, Mrutyunjay Suar
Summary: Staphylococcus aureus infection poses a global threat due to its highly debilitating nature and resistance to antimicrobial medicines. The study identified potential vaccine candidates from the Clumping factor surface proteins and designed a multi-epitope vaccine chimera. Molecular dynamics simulations confirmed the stability of the structure for further analysis.
MICROBIAL PATHOGENESIS
(2021)
Article
Environmental Sciences
Laurynas Vaskevicius, Vilius Malunavicius, Marija Jankunec, Egle Lastauskiene, Martynas Talaikis, Lina Mikoliunaite, Andrius Maneikis, Renata Gudiukaite
Summary: Microbially induced calcite precipitation (MICP) is an efficient and eco-friendly technique that has been applied in various fields. This study aimed to analyze the MICP process of Sporosarcina pasteurii and Staphylococcus sp. H6 and showed the possibility of using Staphylococcus sp. H6 for MICP. The results demonstrated that Staphylococcus sp. H6 precipitated a lower amount of Ca2+ ions compared to S. pasteurii, but both strains showed bioconsolidation of sand particles and reduced water permeability.
ENVIRONMENTAL RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Chong Wang, Huixian Liu, Jiangcai Wang, Yishu Han, Zejun Sun, Haowen Xu, Huan Liu, Dameng Liu, Jianbin Luo
Summary: This paper studies the relationship between non-contact friction and distance on the graphite surface. It is found that energy dissipation increases when the distance is less than 2 nm, showing phonon dissipation. However, when the distance is further decreased, a large friction energy dissipation peak occurs due to hysteretic behavior between surface atom vibrations and tip oscillations.
NANOSCALE ADVANCES
(2022)
Article
Biophysics
Sofiane EL-Kirat-Chatel, Mihayl Varbanov, Chloe Retourney, Elsa Salles, Arnaud Risler, Jean-Michel Brunel, Audrey Beaussart
Summary: This study used atomic force microscopy (AFM) to analyze the morphological changes of Staphylococcus epidermidis after squalamine treatment, revealing the peptidoglycan structure on the bacterial surface. Single-molecule force spectroscopy demonstrated that squalamine binds to the cell surface via the spermidine motif, likely through electrostatic interactions. The study also found the involvement of accumulation-associated protein (Aap) in the initial binding of squalamine to the bacterial cell wall. This work highlights the value of combining AFM with microbiological assays to understand the molecular mechanisms of squalamine's antibacterial activity.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Microbiology
Sheila Thomas, Srishtee Arora, Wen Liu, Kelly Churion, You Wu, Magnus Hook
Summary: Staphylococcus aureus can target various tissues and cause life-threatening infections by spreading in the vascular system throughout the body. The microorganism survives in blood by coating itself with a protective fibrinogen (Fg)/fibrin shield, assembled by Fg-binding bacterial proteins. A novel protein, vWbp homologous protein (vhp), with significant sequence identity to vWbp, was identified in this study, suggesting potential roles in shield assembly. The presence of redundant Fg-binding proteins and the discovery of a common Fg-binding motif highlight the complexity of the interaction between S. aureus and the host's blood coagulation system.
Article
Nanoscience & Nanotechnology
Keivan Asadi, Junghoon Yeom, Hanna Cho
Summary: Investigating internal resonance (IR) mechanisms in micro/nanoresonators reveals that intermodal coupling between second and third flexural modes in asymmetric structures provides an optimal condition for strong IR, with high energy transfer to the resonated mode. This study introduces design strategies that can be easily integrated into typical micro/nanoelectromechanical systems, offering potential for paradigm-shifting applications in micro/nanosystems.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Chemistry, Physical
Mert Efe, Bharat Gwalani, Jinhui Tao, Miao Song, Tiffany C. Kaspar, Arun Devaraj, Aashish Rohatgi
Summary: The study demonstrates the practical application of shear deformation at the nanoscale using atomic force microscopy (AFM) for nanomechanical scratching in single-crystal copper. The results show the ability of AFM to induce local shear deformation and study its effects on the microstructure, with the material removal process through cutting mode enabling the use of stress and strain models developed for bulk machining.
APPLIED SURFACE SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
D. P. Wang, H. T. Zhang, P. Y. Guo, B. A. Sun, Y. X. Wang
Summary: In a Cu50Zr50 metallic glass, local energy dissipation near the shear band plane was characterized using an amplitude-modulation atomic force microscope. The shear band plane exhibited higher energy dissipation compared to the undeformed metallic glass surface. Energy dissipation along the propagating direction of shear bands was periodically distributed due to density changes and discontinuous growth of shear offset during the sliding process.
SCRIPTA MATERIALIA
(2021)
Article
Microbiology
Sven C. J. van Dun, Marielle Verheul, Bart G. C. W. Pijls, Joffrey van Prehn, Henk Scheper, Federica Galli, Peter H. H. Nibbering, Mark G. J. de Boer
Summary: A study investigated the topography of biofilms on different implant materials and their correlation with susceptibility to antimicrobial treatment. The results showed that the surface parameters of MRSA biofilms were determined by the characteristics of the implant materials. There were no significant differences in antibiotic susceptibility among MRSA biofilms on different implant materials.
FRONTIERS IN MICROBIOLOGY
(2023)
Review
Microbiology
Peter N. Lipke, Stephen A. Klotz, Yves F. Dufrene, Desmond N. Jackson, Melissa C. Garcia-Sherman
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS
(2018)
Article
Chemistry, Multidisciplinary
Valeria Prystopiuk, Cecile Feuillie, Philippe Herman-Bausier, Felipe Viela, David Alsteens, Giampiero Pietrocola, Pietro Speziale, Yves F. Dufrene
Article
Medicine, General & Internal
T. Bjarnsholt, K. Buhlin, Y. F. Dufrene, M. Gomelsky, A. Moroni, M. Ramstedt, K. P. Rumbaugh, T. Schulte, L. Sun, B. Akerlund, U. Romling
JOURNAL OF INTERNAL MEDICINE
(2018)
Article
Biochemistry & Molecular Biology
Victoria Vassen, Claire Valotteau, Cecile Feuillie, Cecile Formosa-Dague, Yves F. Dufrene, Xavier De Bolle
Article
Biochemistry & Molecular Biology
Giampiero Pietrocola, Giulia Nobile, Mariangela J. Alfeo, Timothy J. Foster, Joan A. Geoghegan, Vincenzo De Filippis, Pietro Speziale
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Microbiology
Keenan A. Lacey, Michelle E. Mulcahy, Aisling M. Towell, Joan A. Geoghegan, Rachel M. McLoughlin
Article
Microbiology
Amy C. Pickering, Pauline Vitry, Valeriia Prystopiuk, Brandon Garcia, Magnus Hook, Jeffrey Schoenebeck, Joan A. Geoghegan, Yves F. Dufrene, J. Ross Fitzgerald
Article
Multidisciplinary Sciences
Samir Olatunji, Xiaoxiao Yu, Jonathan Bailey, Chia-Ying Huang, Marta Zapotoczna, Katherine Bowen, Maja Remskar, Rolf Mueller, Eoin M. Scanlan, Joan A. Geoghegan, Vincent Olieric, Martin Caffrey
NATURE COMMUNICATIONS
(2020)
Editorial Material
Microbiology
Charles J. Dorman, Joan Geoghegan
CURRENT OPINION IN MICROBIOLOGY
(2020)
Article
Multidisciplinary Sciences
Aisling M. Towell, Cecile Feuillie, Pauline Vitry, Thaina M. Costa, Marion Mathelie-Guinlet, Sanja Kezic, Orla M. Fleury, Maeve A. McAleer, Yves F. Dufrene, Alan D. Irvine, Joan A. Geoghegan
Summary: Staphylococcus aureus interacts with the host protein corneodesmosin on atopic dermatitis corneocytes, taking advantage of the aberrant display of corneodesmosin caused by low natural moisturizing factor levels. The bacterial proteins FnBPB and ClfB bind to the N-terminal region of corneodesmosin, facilitating strong adherence of S. aureus to atopic dermatitis corneocytes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Thaina M. da Costa, Albertus Viljoen, Aisling M. Towell, Yves F. Dufrene, Joan A. Geoghegan
Summary: This study identifies a novel loricrin-binding protein, FnBPB, in Staphylococcus aureus and demonstrates through single-molecule force spectroscopy that FnBPB strengthens the adhesion of S. aureus to human corneocytes through its strong binding to loricrin.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Filippo Prencipe, Aishah Alsibaee, Zainab Khaddem, Padraig Norton, Aisling M. Towell, Afnan F. M. Ali, Gerard Reid, Orla M. Fleury, Timothy J. Foster, Joan A. Geoghegan, Isabel Rozas, Marian P. Brennan
Summary: Colonization of S. aureus can lead to systemic infections, which are difficult to treat due to antibiotic resistance. We have identified a small molecule inhibitor of the S. aureus clumping factors that has potential as a colonization inhibitor.
MICROBIOLOGY SPECTRUM
(2022)
Article
Nanoscience & Nanotechnology
Telmo O. Paiva, Albertus Viljoen, Thaina M. da Costa, Joan A. Geoghegan, Yves F. Dufrene
Summary: Attachment of Staphylococcus aureus to human skin corneocyte cells is mediated by bacterial cell-surface protein adhesins, including fibronectin-binding protein B (FnBPB). Using single-molecule experiments, it is demonstrated that FnBPB binds to corneodesmosin (CDSN) on atopic dermatitis patient corneocytes through a sophisticated two-site mechanism.
ACS NANOSCIENCE AU
(2023)
Article
Materials Science, Biomaterials
Marina Martinez-Carmona, Carmela Cela, Vera A. Kuznetsova, Joan A. Geoghegan, Yurii K. Gun'ko
Summary: The research demonstrates that chirality significantly affects the interaction of mesoporous silica nanoparticles with different cells and bacteria at the cellular level. Understanding the role of chirality in nanoparticles is crucial for the advancement of nano-biotechnology.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)