Article
Nanoscience & Nanotechnology
Chun Cao, Xiaoming Shen, Shixiong Chen, Minfei He, Hongqing Wang, Chenliang Ding, Dazhao Zhu, Jianjie Dong, Hongzheng Chen, Ning Huang, Cuifang Kuang, Ming Jin, Xu Liu
Summary: Two-photon polymerization based direct laser writing (DLW) is a promising micronano 3D fabrication technology, and the use of liquid two-photon initiators (TPIs) enhances its efficiency. The liquid TPIs have higher solubility and absorption cross section than traditional TPIs, resulting in improved polymerization and the ability to fabricate high-quality microstructures.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Multidisciplinary
Jaepil Jeong, Xiaolei Hu, Hironobu Murata, Grzegorz Szczepaniak, Marta Rachwalak, Anna Kietrys, Subha R. R. Das, Krzysztof Matyjaszewski
Summary: Combining synthetic polymers with RNA allows for creating RNA-based materials with unique functions. By developing an acylation reagent, we achieved the direct incorporation of an atom transfer radical polymerization (ATRP) initiator into both synthetic oligoribonucleotides and natural biomass RNA. This approach enables the grafting of polymer chains from RNA and the synthesis of RNA-polymer hybrids with narrow molecular weight distributions, including RNA bottlebrushes, hydrogels, and stimuli-responsive materials.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biochemistry & Molecular Biology
Irina A. Paun, Bogdan S. Calin, Cosmin C. Mustaciosu, Eugenia Tanasa, Antoniu Moldovan, Agata Niemczyk, Maria Dinescu
Summary: Hierarchical structures designed and fabricated via two-photon polymerization by laser direct writing demonstrate cell-repellency capability, with NMP structures more effective in preventing cell attachment and influencing cell shape compared to MMP structures, primarily governed by the structures' topography rather than wettability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Atsushi Nakayama, Yasuaki Kumamoto, Masafumi Minoshima, Kazuya Kikuchi, Atsushi Taguchi, Katsumasa Fujita
Summary: This study demonstrates the use of photopolymerization without photoinitiators to fabricate 3D structures using biocompatible materials. The material properties of poly(ethylene glycol) diacrylate (PEGda) and collagen are preserved, making them suitable for applications with live cells.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Wen Gao, Hao Chao, Yong-Chao Zheng, Wei-Cai Zhang, Jie Liu, Feng Jin, Xian-Zi Dong, Yan-Hong Liu, Shu-Jing Li, Mei-Ling Zheng
Summary: A new kind of ionic carbazole water-soluble photoinitiator was developed for the fabrication of a 3D hydrogel structure in aqueous phase. Through the use of a CB7/BMVMC complex as the photoinitiator, a low laser threshold and high resolution for two-photon polymerization in aqueous phase were achieved. The biocompatibility of the BMVMC-CB7 complex and photoresist was demonstrated through culturing with living cells, showing potential for further application in tissue engineering.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Polymer Science
Xing Huang, Yuxi Zhang, Mengquan Shi, Li-Peng Zhang, Yunlong Zhang, Yuxia Zhao
Summary: A highly biocompatible bio-ink containing a hydrophilic photoinitiator and modified gelatin was used to fabricate 3D hydrogel scaffolds with mouse fibroblast cells via two-photon polymerization. The cells showed vigorous proliferation within the scaffolds after four days of incubation, suggesting great potential for 3D bioprinting.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Jian-Yu Wang, Feng Jin, Xian-Zi Dong, Jie Liu, Ming-Xia Zhou, Teng Li, Mei-Ling Zheng
Summary: This study demonstrates a dual-stimuli cooperative responsive hydrogel microactuator by asymmetric fabrication via femtosecond laser direct writing. The dual response of the hydrogel microstructure is achieved through the use of responsive hydrogel with functional monomer 2-(dimethylamino)ethyl methacrylate. Raman spectra of the hydrogel microstructures suggest that the pH and temperature response of the hydrogel is generated by the changes in tertiary amine groups and hydrogen bonds, respectively. The asymmetric hydrogel microstructures show opposite bending direction when being heated to high temperature or exposed to acid solution, and can independently accomplish the grasp of polystyrene microspheres.
Article
Chemistry, Physical
Purnima N. Manghnani, Valentina Di Francesco, Carlo Panella La Capria, Michele Schlich, Marco Elvino Miali, Thomas Lee Moore, Alessandro Zunino, Marti Duocastella, Paolo Decuzzi
Summary: By utilizing two-photon polymerization in microfluidic channels, anisotropic PEGDA micro-hydrogels in the 1-10 μm size-range can be fabricated, carrying payloads of various nature. This approach could support the fabrication of micro-hydrogels with well-defined morphology, stiffness, and surface properties for sustained release of therapeutic agents.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Engineering, Manufacturing
Caroline Arnoux, Luis A. Perez-Covarrubias, Alexandre Khaldi, Quentin Carlier, Patrice L. Baldeck, Kevin Heggarty, Akos Banyasz, Cyrille Monnereau
Summary: This paper investigates the proximity effects in additive manufacturing and proposes a parallel write approach to overcome the limitations caused by these effects. Through experiments and computer simulations, the authors successfully model the effects and demonstrate the effectiveness of the parallel write approach in fabricating structures with submicronic inter-object distances.
ADDITIVE MANUFACTURING
(2022)
Article
Chemistry, Multidisciplinary
Mehdi Belqat, Xingyu Wu, Jason Morris, Karine Mougin, Tatiana Petithory, Laurent Pieuchot, Yohann Guillaneuf, Didier Gigmes, Jean-Louis Clement, Arnaud Spangenberg
Summary: Photoactivated Reversible Deactivation Radical Polymerization (RDRP) technologies have recently emerged in the field of 3D printing systems, specifically in vat-photopolymerization-based processes such as digital light processing (DLP). Unlike conventional free radical photopolymerization, photoRDRP in 3D printing allows for post-modification of 3D objects. This study develops a photoresist suitable for 3D direct laser writing (3D DLW) based on nitroxide mediated photopolymerization (NMP2) and demonstrates highly tunable, precise, and successive surface patterning of 2D and 3D multi-material microstructures.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Colm Delaney, Jing Qian, Xia Zhang, Radislav Potyrailo, A. Louise Bradley, Larisa Florea
Summary: Arrays of optically responsive ionogel structures were fabricated using direct laser writing, showing visible color changes in the presence of different solvent vapours to demonstrate their responsive nature. This represents a new departure in photonic structural colouration, with the fabricating structure exhibiting programmable, controllable, and dynamic stimuli response.
JOURNAL OF MATERIALS CHEMISTRY C
(2021)
Article
Multidisciplinary Sciences
Jung Y. Han, Sarah Warshawsky, Don L. DeVoe
Summary: The new method of in situ photografting during direct laser writing using two-photon polymerization offers a powerful approach to forming covalent bonds between 3D photoresist structures and thermoplastic surfaces. When applied to microfluidic device fabrication, it can create exceptionally strong adhesion and robust bond interfaces capable of withstanding pressure gradients as high as 7 MPa through proper channel design, photoinitiator selection, and processing conditions.
SCIENTIFIC REPORTS
(2021)
Article
Polymer Science
Martyna Durko-Maciag, Gilles Ulrich, Julien Massue, Jaroslaw Mysliwiec, Konrad Cyprych
Summary: This work demonstrates the successful application of Excited-State Intramolecular Proton Transfer (ESIPT) fluorophores as photoinitiators for microfabrication. The influence of chemical structure on this application was investigated, followed by analysis of two-photon polymerization. The study determined the quality of fabricated structures and polymerization thresholds, with one of the chromophores having an impressively low value of 11 nJ.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Jiaxi Song, Christos Michas, Christopher S. Chen, Alice E. White, Mark W. Grinstaff
Summary: DLW-fabricated hydrogel microstructures with alternating soft and stiff regions enable precise control over cell alignment and selective adhesion of human mesenchymal stem cells. Additionally, DLW patterning allows cell alignment on flat surfaces as well as irregular and curved 3D microstructures, which are typically challenging to pattern with cells.
MACROMOLECULAR BIOSCIENCE
(2021)
Article
Engineering, Biomedical
Teng Li, Jie Liu, Min Guo, Fan-Chun Bin, Jian-Yu Wang, Atsushi Nakayama, Wei-Cai Zhang, Feng Jin, Xian-Zi Dong, Katsumasa Fujita, Mei-Ling Zheng
Summary: This study fabricated protein hydrogels with excellent mechanical properties, high precision, and 3D architecture using modified bovine serum albumin (BSA) and a water-soluble free radical type I photoinitiator. The BSA-GMA hydrogel structure showed excellent autofluorescence imaging, pH responsiveness, and biocompatibility, providing new avenues for potential applications in tissue engineering and biomedical fields to meet specific biological requirements.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Physics, Applied
Rabia Ahmad, M. Shahid Rafique, Ammar Ahmed, Ali Ajami, Pavla Nekvindova, Blanka Svecova, Shazia Bashir, Saman Iqbal
LASER AND PARTICLE BEAMS
(2019)
Article
Nuclear Science & Technology
Lenka Prochazkova, Iveta Terezie Pelikanova, Eva Mihokova, Roman Dedic, Vaclav Cuba
RADIATION MEASUREMENTS
(2019)
Article
Materials Science, Multidisciplinary
Wolfgang Steiger, Peter Gruber, Dominik Theiner, Agnes Dobos, Markus Lunzer, Jasper Van Hoorick, Sandra Van Vlierberghe, Robert Liska, Aleksandr Ovsianikov
OPTICAL MATERIALS EXPRESS
(2019)
Article
Materials Science, Multidisciplinary
Somayeh Najafi, Atoosa Sadat Arabanian, Reza Massudi, Aliasghar Ajami, Wolfgang Husinsky
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
(2020)
Article
Optics
Shazia Bashir, Muhammad Shahid Rafique, Chandra Sekher Nathala, Ali Asghar Ajami, Wolfgang Husinsky, K. Whitmore
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA B-OPTICAL PHYSICS
(2020)
Article
Engineering, Biomedical
Agnes Dobos, Franziska Gantner, Marica Markovic, Jasper Van Hoorick, Liesbeth Tytgat, Sandra Van Vlierberghe, Aleksandr Ovsianikov
Summary: The integration of 3D cell culture techniques with microfluidic approaches in organ-on-chip devices has the potential to overcome the limitations of classical 2D platforms. The use of two-photon polymerization (2PP) allows for high-definition bioprinting of microvascular structures directly on-chip, addressing the challenge of vascularization in fabricated 3D models. The optimization of 2PP printing parameters enables high-throughput production of microfluidic vessel-on-chip platforms with well-defined channels of small diameters.
Article
Polymer Science
Markus Lunzer, Boris Maryasin, Tommaso Zandrini, Stefan Baudis, Aleksandr Ovsianikov, Robert Liska
Summary: Photolabile groups are important components in photo-responsive polymers. Disulfides can be used for facile crosslinking and localized cleavage by two-photon irradiation, but their formation is challenging. The study identifies a reactive lead compound and investigates the effects of the molecular linker structure on hydrogel stability.
Article
Engineering, Biomedical
Olivier Guillaume, Oliver Kopinski-Gruenwald, Gregor Weisgrab, Theresia Baumgartner, Aysu Arslan, Karin Whitmore, Sandra Van Vlierberghe, Aleksandr Ovsianikov
Summary: Tissue engineering and regenerative medicine (TERM) has previously relied on scaffold-based or scaffold-free strategies, but a new approach called the third TERM strategy, involving highly porous microscaffolds and single spheroids, has the potential to overcome limitations of previous options. A systematic study comparing microscaffold-based tissue units with conventional spheroids demonstrated that the microscaffold did not impede spheroid formation or impact viability. Additionally, the fusion and differentiation potential necessary for TERM applications were preserved. Microscaffold-based tissue units offer benefits such as enhanced cell retention, decreased compaction, and better size control for tissue constructs formed through self-assembly.
ACTA BIOMATERIALIA
(2023)
Review
Biotechnology & Applied Microbiology
Tommaso Zandrini, Sammy Florczak, Riccardo Levato, Aleksandr Ovsianikov
Summary: Bioprinting aims to create 3D structures with embedded cells that mimic the native extracellular matrix, but limited spatial resolution has been a hindrance. This opinion article reviews the emergence and discusses the most promising high-definition (HD) bioprinting techniques to overcome this limitation and enable the fabrication of more complex tissue and organ models in tissue engineering.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Gregor Weisgrab, Olivier Guillaume, Zhengchao Guo, Patrick Heimel, Paul Slezak, Andre Poot, Dirk Grijpma, Aleksandr Ovsianikov
Article
Engineering, Biomedical
Tanchen Ren, Wolfgang Steiger, Pu Chen, Aleksandr Ovsianikov, Utkan Demirci
Review
Engineering, Biomedical
Jasper Van Hoorick, Liesbeth Tytgat, Agnes Dobos, Heidi Ottevaere, Jurgen Van Erps, Hugo Thienpont, Aleksandr Ovsianikov, Peter Dubruel, Sandra Van Vlierberghe
ACTA BIOMATERIALIA
(2019)
Article
Materials Science, Biomaterials
Liesbeth Tytgat, Marica Markovic, Taimoor H. Qazi, Maxime Vagenende, Fabrice Bray, Jose C. Martins, Christian Rolando, Hugo Thienpont, Heidi Ottevaere, Aleksandr Ovsianikov, Peter Dubruel, Sandra Van Vlierberghe
JOURNAL OF MATERIALS CHEMISTRY B
(2019)
Article
Chemistry, Physical
Brigitte Holzer, Markus Lunzer, Arnulf Rosspeintner, Giuseppe Licari, Maximilian Tromayer, Sergej Naumov, Daniel Lumpi, Ernst Horkel, Christian Hametner, Aleksandr Ovsianikov, Robert Liska, Eric Vauthey, Johannes Froehlich
MOLECULAR SYSTEMS DESIGN & ENGINEERING
(2019)
Article
Chemistry, Analytical
Agnes Dobos, Wolfgang Steiger, Dominik Theiner, Peter Gruber, Markus Lunzer, Jasper Van Hoorick, Sandra Van Vlierberghe, Aleksandr Ovsianikov
Article
Polymer Science
Paige A. Shaw, Maxime Klausen, Mark Bradley
Summary: A light-responsive polymer capable of controlled release of camptothecin and generation of reactive oxygen species (ROS) was developed, which exhibited high cancer cell killing activity through both camptothecin release and ROS generation.
Article
Polymer Science
Donghui Zhang, Shiqi Liu, Zhihao Cai, Weizhe Luo, Guojian Liu, Runhui Liu
Summary: A straightforward and efficient method for synthesizing NPC monomers has been developed in this study, which enhances the availability of NPC monomers and facilitates the synthesis of polypeptides.