Review
Biotechnology & Applied Microbiology
Dielle Pierotti Procopio, Emanuele Kendrick, Rosana Goldbeck, Andre Ricardo de Lima Damasio, Telma Teixeira Franco, David J. Leak, Yong-Su Jin, Thiago Olitta Basso
Summary: The engineering of xylo-oligosaccharide-consuming Saccharomyces cerevisiae strains is a promising approach for the effective utilization of lignocellulosic biomass and development of economic industrial fermentation processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yixuan Zhu, Jingtao Zhang, Lang Zhu, Zefang Jia, Qi Li, Wei Xiao, Limin Cao
Summary: Efficient xylose fermentation in budding yeast was achieved through rational promoter elements engineering, with HXT7 showing the best performance among surveyed promoters. The redox balance of the xylose utilization pathway was optimized to achieve a balanced xylose metabolism toward ethanol formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lulu Liu, Mingjie Jin, Mingtao Huang, Yixuan Zhu, Wenjie Yuan, Yingqian Kang, Meilin Kong, Sajid Ali, Zefang Jia, Zhaoxian Xu, Wei Xiao, Limin Cao
Summary: The reported haploid Saccharomyces cerevisiae strain F106 has the ability to utilize xylose for ethanol production, with the XR-K270R mutant showing the best performance after a series of mutations. The study found that the yeast chromosomal copy number is positively correlated with increased ethanol production under the experimental conditions, indicating the potential for improving ethanol yield through genetic modifications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Gabriel de Souza Colombo, Isis Viana Mendes, Betulia de Morais Souto, Cristine Chaves Barreto, Luana Assis Serra, Eliane Ferreira Noronha, Nadia Skorupa Parachin, Joao Ricardo Moreira de Almeida, Betania Ferraz Quirino
Summary: The current climate crisis calls for the replacement of fossil energy sources with sustainable alternatives. This study presents a new bacterial XI enzyme that has been successfully expressed in Saccharomyces cerevisiae, allowing efficient consumption and metabolism of xylose.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Warasirin Sornlek, Kittapong Sae-Tang, Akaraphol Watcharawipas, Sriwan Wongwisansri, Sutipa Tanapongpipat, Lily Eurwilaichtr, Verawat Champreda, Weerawat Runguphan, Peter J. Schaap, Vitor A. P. Martins Dos Santos
Summary: In this study, the production of D-lactic acid (D-LA) was improved in Saccharomyces cerevisiae by gene editing and conventional breeding. By deleting specific genes and crossbreeding with acid-tolerant yeast strains, the production and yield of D-LA were significantly increased, while the production of glycerol and ethanol were minimized.
Review
Biotechnology & Applied Microbiology
Jian Zha, Miaomiao Yuwen, Weidong Qian, Xia Wu
Summary: Xylose is the second most abundant sugar in lignocellulosic hydrolysates, and utilizing yeast strains for transforming xylose into valuable chemicals is a feasible and sustainable approach. Metabolically engineered yeasts have shown significant progress in producing natural products from xylose, such as aromatics, terpenoids, and flavonoids. Challenges and future perspectives in yeast engineering for commercial production of natural products using xylose as feedstocks are discussed.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Kanghee Han, Hyunjun Lee, Tae-Gu Kang, Jihyun Lee, Sun-Ki Kim
Summary: This study explored the feasibility of using engineered Saccharomyces cerevisiae strains to eliminate ethyl carbamate (EC). By introducing the urethanase gene that can directly convert EC into ethanol and ammonia, the degradation of EC was significantly enhanced. Moreover, displaying the enzyme on the yeast cell surface further improved the degradation activity of EC.
Article
Biotechnology & Applied Microbiology
Sae-Byuk Lee, Mary Tremaine, Michael Place, Lisa Liu, Austin Pier, David J. Krause, Dan Xie, Yaoping Zhang, Robert Landick, Audrey P. Gasch, Chris Todd Hittinger, Trey K. Sato
Summary: The efficient conversion of xylose into biofuels has been limited by bottlenecks, but a new method involving engineered yeast strains has shown promise in aerobically fermenting xylose to ethanol. Through gene duplications and deletions, the rates and yields of xylose fermentation were increased, potentially enabling sustainable production of industrial biofuels. This research offers insights into how key regulatory mutations can prime yeast for aerobic xylose fermentation, leading to increased xylose flux and redirection into fermentation products.
METABOLIC ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Jung-Hoon Bae, Mi-Jin Kim, Bong Hyun Sung, Yong-Su Jin, Jung-Hoon Sohn
Summary: The extracellular conversion of xylose to xylulose before uptake has been found to be effective in xylose fermentation or glucose/xylose co-fermentation, reducing the competition between glucose and xylose for cellular uptake. The engineered XI secretion system developed in this study may enable the simultaneous utilization of C5/C6 sugars from sustainable lignocellulosic biomass.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Multidisciplinary Sciences
Felix H. Lam, Burcu Turanli-Yildiz, Dany Liu, Michael G. Resch, Gerald R. Fink, Gregory Stephanopoulos
Summary: By engineering Saccharomyces cerevisiae, near-parity production can be achieved between inhibitor-laden and inhibitor-free feedstocks, and a single strain can be enhanced to tolerate diverse highly toxified feedstocks. Moreover, a lightweight design allows for seamless transferability to existing metabolically engineered chassis strains, demonstrating the potential for cost-effective, at-scale biomass utilization for cellulosic fuel and nonfuel products.
Article
Agricultural Engineering
Meilin Kong, Xiaowei Li, Tongtong Li, Xuebing Zhao, Mingjie Jin, Xin Zhou, Hanqi Gu, Vladimir Mrsa, Wei Xiao, Limin Cao
Summary: CCW12(OE) is a Saccharomyces cerevisiae strain constructed by overexpressing CCW12, which shows high ethanol production efficiency in various hydrolysates and exhibits better stress resistance and fermentation performance compared to the control strain WXY70.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jana Fees, Jonas J. Christ, Sabine Willbold, Lars M. Blank
Summary: A biotechnological process using Saccharomyces cerevisiae to upgrade phosphate-containing wastewater into pure sodium polyphosphate was developed in this study. The quality of polyphosphate was independent of the composition of the wash water and can be reused in various applications, contributing to the sustainable utilization of phosphate and reducing the need for phosphate rock.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Review
Biochemical Research Methods
Liang Sun, Yong-Su Jin
Summary: The microbial conversion of plant biomass into fuels and chemicals using engineered yeast has shown progress in efficiently converting xylose and co-utilizing it with glucose. Research investments have facilitated the simultaneous fermentation of xylose and glucose, with attention now turning towards improving xylose-utilizing efficiency and achieving robust co-fermentation under industrial conditions. Harnessing the advantageous traits of yeast xylose metabolism is expected to lead to the production of diverse fuels and chemicals.
BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemical Research Methods
Nam Kyu Kang, Jae Won Lee, Donald R. Ort, Yong-Su Jin
Summary: In this study, engineered Saccharomyces cerevisiae was utilized to produce malic acid from xylose, with increased malic acid yield and productivity achieved through a low-level xylose fed-batch strategy. This work suggests effective strategies for using xylose in the biological production of value-added products.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Sriram Vijayraghavan, Latarsha Porcher, Piotr A. Mieczkowski, Natalie Saini
Summary: Acetaldehyde is a by-product of ethanol metabolism and is acutely toxic and carcinogenic. In vitro studies have shown its mutagenic effects on DNA, but there is variability in reporting its mutagenicity in vivo. This study used a yeast genetic reporter system and found that acetaldehyde treatment is highly mutagenic and leads to specific mutations on single stranded DNA.
NUCLEIC ACIDS RESEARCH
(2022)