Journal
PLOS ONE
Volume 13, Issue 1, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pone.0186782
Keywords
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Categories
Funding
- National Institute of Allergy and Infectious Diseases, National Institutes of Health [1R21AI111120-01A1]
- National Science Foundation [CBET-1511789]
- Cancer Prevention & Research Institute of Texas [RP150343]
- Fundacao para Ciencia e Tecnologia (FCT) [PD/BD/105883/2014, SFRH/BD/91099/2012, IF/00048/2014/CP1214/CT0010]
- FCT [UID/BIO/04565/2013]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/91099/2012, IF/00048/2014/CP1214/CT0010, PD/BD/105883/2014] Funding Source: FCT
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Lateral flow assays (LFAs) are a widely-used point-of care diagnostic format, but suffer from limited analytical sensitivity, especially when read by eye. It has recently been reported that LFA performance can be improved by using magnetic reporter particles and an external magnetic field applied at the test line. The mechanism of sensitivity/performance enhancement was suggested to be concentration/retardation of reporter particles at the test line. Here we demonstrate an additional mechanism of particle relocation where reporter particles from the lower depths of the translucent LFA strip relocate to more-visible locations nearer to the top surface, producing a more visible signal. With a magnetic field we observed an improvement in sensitivity of human chorionic gonadotropin (hCG) detection from 1.25 ng/mL to 0.31 ng/mL. We also observed an increase of the color intensity per particle in test lines when the magnetic field was present.
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